Objective:To evaluate the effect of hydroxysafflor yellow A(HSYA)on thioacetamide-induced liver fibrosis.Methods:Thioacetamide was administered to rats intraperitoneally in doses of 200 mg/kg twice a week for 12 weeks...Objective:To evaluate the effect of hydroxysafflor yellow A(HSYA)on thioacetamide-induced liver fibrosis.Methods:Thioacetamide was administered to rats intraperitoneally in doses of 200 mg/kg twice a week for 12 weeks.Thioacetamide-intoxicated rats were given silymarin(50 mg/kg)or HSYA(5 mg/kg)orally every day for 8 weeks.Liver enzymes,fibrosis markers,histological changes as well as immunohistochemistry of TNF-α,IL-6,p21,α-SMA,and caspase-3 were examined.The effect of HSYA on HSC-T6 activation/proliferation and apoptosis was also determined in vitro.Results:HSYA decreased liver enzymes,TNF-α,IL-6,and p21 expressions,hepatic PDGF-B,TIMP-1,TGF-β1,and hydroxyproline levels,as well as fibrosis score(S2 vs.S4)compared to the thioacetamide group.HSYA also downregulatedα-SMA while increasing caspase-3 expression.Surprisingly,at 500μg/mL,HSYA had only a slightly suppressive effect on HSC proliferation,with a 9.5%reduction.However,it significantly reduced TGF-β1,inhibitedα-SMA expression,induced caspase-3 expression,and promoted cell senescence.Conclusions:HSYA may be a potential therapeutic agent for delaying and reversing the progression of liver fibrosis.More research on HSYA at higher doses and for a longer period is warranted.展开更多
An investigation was carried out on living coccolithophores （LCs） distribution in the Yellow Sea and the East China Sea from October 17 to November 24, 2011. A total of 223 samples from different depths were col-lec...An investigation was carried out on living coccolithophores （LCs） distribution in the Yellow Sea and the East China Sea from October 17 to November 24, 2011. A total of 223 samples from different depths were col-lected at 48 stations. Totally 18 taxa belonging to coccolithophyceae were identified using a polarized micro-scope at the 1 000× magnification. The maximum species abundance was found at the outside of Transect P. The dominated species wereGephyrocapsa oceanica, Emiliania huxleyi,Helicosphaera carteri,andAlgiros-phaera robusta.The abundance of coccoliths and cells ranged 0-2 965.73 coccoliths/mL, and 0-119.16 cells/mL, with the average values of 471.00 coccoliths/mL and 23.42 cells/mL, respectively. The LCs in surface layer were mainly observed on the coastal belt and middle part of the survey area. The comparison among Transects A, F, P and E indicated lower species diversity and less abundance in the Yellow Sea than those of the East China Sea. The highest abundance of LCs was found in transect F and P. The coccolith abundance increased slightly from surface to bottom in the water column, but the highest value of the cell abundance was observed in the depth of 10-30 m. Temperature, depth and nutrient concentration were suggested as the major environmental factors controlling the distribution and species composition of LCs in the studying area based on canonical correspondence analysis （CCA）.展开更多
Degradable plastic mulch is being used to overcome the negative environmental impacts of burning and landfilling agricultural plastic waste. In this study P. ostreatus was used to model the capacity of a vegetal speci...Degradable plastic mulch is being used to overcome the negative environmental impacts of burning and landfilling agricultural plastic waste. In this study P. ostreatus was used to model the capacity of a vegetal species to degrade conventional and degradable plastic films. Plastics studied were oxo-degradable polyethylene (OXO-PE), UV-irradiated oxo-degradable polyethylene (UV-OXO-PE), polylactic acid (PLA) and conventional polyethylene (C-PE). The cultivation of P. ostreatus resulted in a reduction in the median of weight (78.2% - 80.2%) and volume (56.1% - 60.1%) of the substrate (wheat straw). Degradation of the plastics embodied was evidenced by a reduction in the median of the elongation at break (OXO-PE 475% to 109%, UV-OXO-PE 23% to 8%, PLA 596% to 398% and C-PE 505% to 304%) and an increase in the median of the carbonyl index (OXO-PE 0.062 to 0.114, UV-OXO-PE 0.098 to 0.145 and PLA 0.024 to 0.034). The Kruskal-Wallis test found no statistical difference (p = 0.384) between the medians of the biological efficiency for substrates containing plastics and the substrate without plastic. In conclusion, plastics embodied in the substrates used for cultivation of P. ostreatus are degraded and the degradation of these plastics does not affect the short term growth of P. ostreatus.展开更多
The relationship between P (spring tidal prism) and A (throat area below mean sea level) is statistically analysed in terms of 29 tidal inlets or bays along the Huanghai Sea (Yellow Sea) and Bohai Sea coasts. For 15 o...The relationship between P (spring tidal prism) and A (throat area below mean sea level) is statistically analysed in terms of 29 tidal inlets or bays along the Huanghai Sea (Yellow Sea) and Bohai Sea coasts. For 15 of these tidal inlets, the best regression equation is A(km2) = 0.845 />(km3)1.20. The analysis shows that C and n are little different from those in the P-A relationship for the inlets of the South China Sea and East China Sea coasts. It is noted that the relationship between P and A is unstable because of the difference in sediment abundance. The study shows that a united P-A relationship can be obtained for the tidal inlets of lagoon type and bay-drowned-valley type, not containing some half-circle shape bays which confront deep water. These half-circle bays do not belong to tidal inlets because they do not have enough sediment abundance and are fairly open.展开更多
Study of horizontal and vertical distributions of the N/P (nitrogen versus phosphate) atom ratio in the northern South Yellow Sea showed that the ratio varied greatly in upper waters of the investigated area and was a...Study of horizontal and vertical distributions of the N/P (nitrogen versus phosphate) atom ratio in the northern South Yellow Sea showed that the ratio varied greatly in upper waters of the investigated area and was always much greater than the theoretical Redfield ratio of 16:1 found below the thermocline zone. It was in general higher near the coast and lower in the central part. With increasing depth, the ratio became smaller and smaller. This distribution pattern is attributed to: 1) the anthropogenic influence of the surface N and P which makes the N/P ratio differ from the normal value; 2) the easy adsorption of P on particles hinders P transport to the central part; 3) below the thermocline zone, the N and P mainly come from the remineralization of the sedimented phytoplankton residues which have almost the theoretical Redfield value and; 4) the existence of the Yellow Sea Bottom Cold Water which inhibits the vertical exchange of the water.展开更多
[ Objectivel The study aims to discuss the effects of metal ions on the activity of Pleurotus ostreatus and Pleurotus eryngii cellulose. [ Method] 0.5 mmol/L FeSO4, CuSO4, MnSO4 and ZnSO4 solutions were added to the b...[ Objectivel The study aims to discuss the effects of metal ions on the activity of Pleurotus ostreatus and Pleurotus eryngii cellulose. [ Method] 0.5 mmol/L FeSO4, CuSO4, MnSO4 and ZnSO4 solutions were added to the basal culture media containing silkworm excrement and rice husk, and the effects of metal ions on activity of FPA and Cx enzyme produced by P. ostreatus and P. eryngiiwere analyzed. [ Resultl The addi- tion of Zn2. and Mn~＊ to the basal culture media containing silkworm excrement and rice husk was beneficial to the improvement of the activity of FPA and Cx enzyme produced by P. ostreatus and P. eryngii, while the addition of Fe2＋ and Cu2. inhibited the activity of the cellulase. EConclu- sion I The research could provide theoretical references for improvement of utilization rate of cellulase in silkworm excrement and rice husk.展开更多
Soil phosphorus release to the water bodies in the upland fields of yellow soil areas and impacting factor was studied in Guizhou province. The results showed that the content of dissolved active P of surface runoff f...Soil phosphorus release to the water bodies in the upland fields of yellow soil areas and impacting factor was studied in Guizhou province. The results showed that the content of dissolved active P of surface runoff from various upland fields of yellow-soil were significantly different, which the concentrations of dissolved active P of runoff correlated with the contents of available-P, amorphous oxides of A1, and organic matter in the soils. The amount of soil phosphorus release to the water bodies affected by the level of applying P fertilizer and the process of corn growth, which with fertilizing from 150 to 900 kg P205 ha^-1 in the soil with high P level, the average contents of dissolved active P in the permeability-water of the soil increased from 0.020 mg L^-1 to 0.137 mg L^-1. The amount of soil phosphorus release to the water bodies also affected by environmental factor, which the amount of soil phosphorus release significantly increased under the conditions that temperature is 30℃-35℃, water/soil ratios is 15：1-25：1, submergence-time by water is 12-18 h and pH value of acid rains is 3.82-3.73.展开更多
基金funded by Theodore Bilharz Research Institute (grant number:ID-MS-99/A,Principal investigator:Naglaa M.El-Lakkany).
文摘Objective:To evaluate the effect of hydroxysafflor yellow A(HSYA)on thioacetamide-induced liver fibrosis.Methods:Thioacetamide was administered to rats intraperitoneally in doses of 200 mg/kg twice a week for 12 weeks.Thioacetamide-intoxicated rats were given silymarin(50 mg/kg)or HSYA(5 mg/kg)orally every day for 8 weeks.Liver enzymes,fibrosis markers,histological changes as well as immunohistochemistry of TNF-α,IL-6,p21,α-SMA,and caspase-3 were examined.The effect of HSYA on HSC-T6 activation/proliferation and apoptosis was also determined in vitro.Results:HSYA decreased liver enzymes,TNF-α,IL-6,and p21 expressions,hepatic PDGF-B,TIMP-1,TGF-β1,and hydroxyproline levels,as well as fibrosis score(S2 vs.S4)compared to the thioacetamide group.HSYA also downregulatedα-SMA while increasing caspase-3 expression.Surprisingly,at 500μg/mL,HSYA had only a slightly suppressive effect on HSC proliferation,with a 9.5%reduction.However,it significantly reduced TGF-β1,inhibitedα-SMA expression,induced caspase-3 expression,and promoted cell senescence.Conclusions:HSYA may be a potential therapeutic agent for delaying and reversing the progression of liver fibrosis.More research on HSYA at higher doses and for a longer period is warranted.
基金The National Program on Key Basic Research Project of China under contract Nos 2010CB428900 and 2009CB421202the National Natural Science Foundation of China under contract Nos 41176136,40776093,41276124,41050110436 and 41306118
文摘An investigation was carried out on living coccolithophores （LCs） distribution in the Yellow Sea and the East China Sea from October 17 to November 24, 2011. A total of 223 samples from different depths were col-lected at 48 stations. Totally 18 taxa belonging to coccolithophyceae were identified using a polarized micro-scope at the 1 000× magnification. The maximum species abundance was found at the outside of Transect P. The dominated species wereGephyrocapsa oceanica, Emiliania huxleyi,Helicosphaera carteri,andAlgiros-phaera robusta.The abundance of coccoliths and cells ranged 0-2 965.73 coccoliths/mL, and 0-119.16 cells/mL, with the average values of 471.00 coccoliths/mL and 23.42 cells/mL, respectively. The LCs in surface layer were mainly observed on the coastal belt and middle part of the survey area. The comparison among Transects A, F, P and E indicated lower species diversity and less abundance in the Yellow Sea than those of the East China Sea. The highest abundance of LCs was found in transect F and P. The coccolith abundance increased slightly from surface to bottom in the water column, but the highest value of the cell abundance was observed in the depth of 10-30 m. Temperature, depth and nutrient concentration were suggested as the major environmental factors controlling the distribution and species composition of LCs in the studying area based on canonical correspondence analysis （CCA）.
文摘Degradable plastic mulch is being used to overcome the negative environmental impacts of burning and landfilling agricultural plastic waste. In this study P. ostreatus was used to model the capacity of a vegetal species to degrade conventional and degradable plastic films. Plastics studied were oxo-degradable polyethylene (OXO-PE), UV-irradiated oxo-degradable polyethylene (UV-OXO-PE), polylactic acid (PLA) and conventional polyethylene (C-PE). The cultivation of P. ostreatus resulted in a reduction in the median of weight (78.2% - 80.2%) and volume (56.1% - 60.1%) of the substrate (wheat straw). Degradation of the plastics embodied was evidenced by a reduction in the median of the elongation at break (OXO-PE 475% to 109%, UV-OXO-PE 23% to 8%, PLA 596% to 398% and C-PE 505% to 304%) and an increase in the median of the carbonyl index (OXO-PE 0.062 to 0.114, UV-OXO-PE 0.098 to 0.145 and PLA 0.024 to 0.034). The Kruskal-Wallis test found no statistical difference (p = 0.384) between the medians of the biological efficiency for substrates containing plastics and the substrate without plastic. In conclusion, plastics embodied in the substrates used for cultivation of P. ostreatus are degraded and the degradation of these plastics does not affect the short term growth of P. ostreatus.
基金This work is financially supported by the National Nature Science Fundation of China
文摘The relationship between P (spring tidal prism) and A (throat area below mean sea level) is statistically analysed in terms of 29 tidal inlets or bays along the Huanghai Sea (Yellow Sea) and Bohai Sea coasts. For 15 of these tidal inlets, the best regression equation is A(km2) = 0.845 />(km3)1.20. The analysis shows that C and n are little different from those in the P-A relationship for the inlets of the South China Sea and East China Sea coasts. It is noted that the relationship between P and A is unstable because of the difference in sediment abundance. The study shows that a united P-A relationship can be obtained for the tidal inlets of lagoon type and bay-drowned-valley type, not containing some half-circle shape bays which confront deep water. These half-circle bays do not belong to tidal inlets because they do not have enough sediment abundance and are fairly open.
文摘Study of horizontal and vertical distributions of the N/P (nitrogen versus phosphate) atom ratio in the northern South Yellow Sea showed that the ratio varied greatly in upper waters of the investigated area and was always much greater than the theoretical Redfield ratio of 16:1 found below the thermocline zone. It was in general higher near the coast and lower in the central part. With increasing depth, the ratio became smaller and smaller. This distribution pattern is attributed to: 1) the anthropogenic influence of the surface N and P which makes the N/P ratio differ from the normal value; 2) the easy adsorption of P on particles hinders P transport to the central part; 3) below the thermocline zone, the N and P mainly come from the remineralization of the sedimented phytoplankton residues which have almost the theoretical Redfield value and; 4) the existence of the Yellow Sea Bottom Cold Water which inhibits the vertical exchange of the water.
文摘[ Objectivel The study aims to discuss the effects of metal ions on the activity of Pleurotus ostreatus and Pleurotus eryngii cellulose. [ Method] 0.5 mmol/L FeSO4, CuSO4, MnSO4 and ZnSO4 solutions were added to the basal culture media containing silkworm excrement and rice husk, and the effects of metal ions on activity of FPA and Cx enzyme produced by P. ostreatus and P. eryngiiwere analyzed. [ Resultl The addi- tion of Zn2. and Mn~＊ to the basal culture media containing silkworm excrement and rice husk was beneficial to the improvement of the activity of FPA and Cx enzyme produced by P. ostreatus and P. eryngii, while the addition of Fe2＋ and Cu2. inhibited the activity of the cellulase. EConclu- sion I The research could provide theoretical references for improvement of utilization rate of cellulase in silkworm excrement and rice husk.
文摘Soil phosphorus release to the water bodies in the upland fields of yellow soil areas and impacting factor was studied in Guizhou province. The results showed that the content of dissolved active P of surface runoff from various upland fields of yellow-soil were significantly different, which the concentrations of dissolved active P of runoff correlated with the contents of available-P, amorphous oxides of A1, and organic matter in the soils. The amount of soil phosphorus release to the water bodies affected by the level of applying P fertilizer and the process of corn growth, which with fertilizing from 150 to 900 kg P205 ha^-1 in the soil with high P level, the average contents of dissolved active P in the permeability-water of the soil increased from 0.020 mg L^-1 to 0.137 mg L^-1. The amount of soil phosphorus release to the water bodies also affected by environmental factor, which the amount of soil phosphorus release significantly increased under the conditions that temperature is 30℃-35℃, water/soil ratios is 15：1-25：1, submergence-time by water is 12-18 h and pH value of acid rains is 3.82-3.73.
