Vegetation restoration can alter carbon(C),nitrogen(N),and phosphorus(P)cycles in coastal wetlands affecting C:N:P stoichiometry.However,the effects of restoration age on soil C:N:P stoichiometry are unclear.In this s...Vegetation restoration can alter carbon(C),nitrogen(N),and phosphorus(P)cycles in coastal wetlands affecting C:N:P stoichiometry.However,the effects of restoration age on soil C:N:P stoichiometry are unclear.In this study,we examined the re-sponses of soil C,N,and P contents and their stoichiometric ratios to vegetation restoration age,focusing on below-ground processes and their relationships to aboveground vegetation community characteristics.We conducted an analysis of temporal gradients based on the'space for time'method to synthesize the effects of restoration age on soil C:N:P stoichiometry in the Yellow River Delta wetland of China.The findings suggest that the combined effects of restoration age and soil depth create complex patterns of shifting soil C:N:P stoichiometry.Specifically,restoration age significantly increased all topsoil C:N:P stoichiometries,except for soil total phosphorus(TP)and the C:N ratio,and slightly affected subsoil C:N:P stoichiometry.The effects of restoration age on the soil C:N ratio was well constrained owing to the coupled relationship between soil organic carbon(SOC)and total nitrogen(TN)contents,while soil TP con-tent was closely related to changes in plant species diversity.Importantly,we found that the topsoil C:N:P stoichiometry was signific-antly affected by plant species diversity,whereas the subsoil C:N:P stoichiometry was more easily regulated by pH and electric con-ductivity(EC).Overall,this study shows that vegetation restoration age elevated SOC and N contents and alleviated N limitation,which is useful for further assessing soil C:N:P stoichiometry in coastal restoration wetlands.展开更多
米用454高通量测序（454 high throughput sequencing）分析方法,研究不同覆被（光板地和柽柳群落以及白茅群落）类型下土壤真菌组成、多样性及与理化性质的关系.该研究共获得5门174属真菌,不同土样中真菌种类和多样性有所不同,其中,白...米用454高通量测序（454 high throughput sequencing）分析方法,研究不同覆被（光板地和柽柳群落以及白茅群落）类型下土壤真菌组成、多样性及与理化性质的关系.该研究共获得5门174属真菌,不同土样中真菌种类和多样性有所不同,其中,白茅群落真菌的种类较多,尤其是HZ3A（白茅0.20cm）中真菌属数量最多,其真菌属分别是HZ1A（光板地0.20cm）、HZ1B（光板地20.40cm）、HZ2A（柽柳0.20cm）、HZ2B（柽柳20.40cm）、HZ3B（白茅20.40cm）的2.23、1.74、1.24、2.18和1.38倍.此外,白茅群落真菌多样性高于柽柳群落和光板地.碱解氮对土壤真菌多样性和分布的影响最大,其与Kunfia、旋孢腔菌属（Cochliobo-lus）、毛霉属（Mwcor）、马拉色氏霉菌属（Malassezia）的相对丰度呈负相关,与其余真菌属的相对丰度呈正相关.展开更多
探究土壤真菌碳源代谢水平与盐生植被演替之间的关系,是理清生态系统的内部结构和功能变化的重要步骤。采用Biolog-FF微孔板培养法,结合土壤理化性质分析,研究黄河三角洲地区不同盐生植被下土壤真菌的碳源代谢活性特征。结果表明,在0~2...探究土壤真菌碳源代谢水平与盐生植被演替之间的关系,是理清生态系统的内部结构和功能变化的重要步骤。采用Biolog-FF微孔板培养法,结合土壤理化性质分析,研究黄河三角洲地区不同盐生植被下土壤真菌的碳源代谢活性特征。结果表明,在0~20cm土层,每孔平均吸光值(Average well color development,AWCD值)表现为白茅>罗布麻>柽柳>马绊草>光板地,20~40cm土层则表现为白茅>马绊草>柽柳>罗布麻>光板地,即随盐生植被正向演替,土壤真菌碳源代谢活性显著提高(P<0.05);各样地土壤真菌群落的香农-威尔指数H、丰富度指数S和辛普森指数D按演替顺序均有显著提高,同一样地不同土层中,除马绊草群落,其余样地的3种指数均表现为0~20 cm>20~40cm,说明盐生植被正向演替过程改善了土壤中真菌的多样性和丰富度,提高了群落中常见物种的优势度。土壤理化性质与土壤真菌吸光值、真菌多样性指数相关性分析结果表明,全氮、碱解氮、土壤有机质、磷酸酶和过氧化氢酶显著促进了土壤真菌的碳源代谢活性,土壤含盐量则显著抑制真菌的碳源代谢活性(P<0.05);土壤主成分分析表明,纤维醇、鸟氨酸和D-甘露醇等是0~20 cm土层下土壤真菌利用的主要碳源,D-山梨醇、丙三醇和L-天冬氨酸等则是20~40 cm土层下土壤真菌利用的主要碳源,表明糖类、醇类和酸类是控制土壤真菌碳源代谢发生差异的主要碳源。土壤真菌碳源代谢水平与盐生植被演替关系的结果为黄河三角洲的生态恢复和利用奠定了理论基础。展开更多
基金Under the auspices of Natural Science Foundation of China(No.U2106209,42071126)Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA23050202)International Science Partnership Program of the Chinese Academy of Sciences(No.121311KYSB20190029)。
文摘Vegetation restoration can alter carbon(C),nitrogen(N),and phosphorus(P)cycles in coastal wetlands affecting C:N:P stoichiometry.However,the effects of restoration age on soil C:N:P stoichiometry are unclear.In this study,we examined the re-sponses of soil C,N,and P contents and their stoichiometric ratios to vegetation restoration age,focusing on below-ground processes and their relationships to aboveground vegetation community characteristics.We conducted an analysis of temporal gradients based on the'space for time'method to synthesize the effects of restoration age on soil C:N:P stoichiometry in the Yellow River Delta wetland of China.The findings suggest that the combined effects of restoration age and soil depth create complex patterns of shifting soil C:N:P stoichiometry.Specifically,restoration age significantly increased all topsoil C:N:P stoichiometries,except for soil total phosphorus(TP)and the C:N ratio,and slightly affected subsoil C:N:P stoichiometry.The effects of restoration age on the soil C:N ratio was well constrained owing to the coupled relationship between soil organic carbon(SOC)and total nitrogen(TN)contents,while soil TP con-tent was closely related to changes in plant species diversity.Importantly,we found that the topsoil C:N:P stoichiometry was signific-antly affected by plant species diversity,whereas the subsoil C:N:P stoichiometry was more easily regulated by pH and electric con-ductivity(EC).Overall,this study shows that vegetation restoration age elevated SOC and N contents and alleviated N limitation,which is useful for further assessing soil C:N:P stoichiometry in coastal restoration wetlands.
文摘米用454高通量测序（454 high throughput sequencing）分析方法,研究不同覆被（光板地和柽柳群落以及白茅群落）类型下土壤真菌组成、多样性及与理化性质的关系.该研究共获得5门174属真菌,不同土样中真菌种类和多样性有所不同,其中,白茅群落真菌的种类较多,尤其是HZ3A（白茅0.20cm）中真菌属数量最多,其真菌属分别是HZ1A（光板地0.20cm）、HZ1B（光板地20.40cm）、HZ2A（柽柳0.20cm）、HZ2B（柽柳20.40cm）、HZ3B（白茅20.40cm）的2.23、1.74、1.24、2.18和1.38倍.此外,白茅群落真菌多样性高于柽柳群落和光板地.碱解氮对土壤真菌多样性和分布的影响最大,其与Kunfia、旋孢腔菌属（Cochliobo-lus）、毛霉属（Mwcor）、马拉色氏霉菌属（Malassezia）的相对丰度呈负相关,与其余真菌属的相对丰度呈正相关.
文摘探究土壤真菌碳源代谢水平与盐生植被演替之间的关系,是理清生态系统的内部结构和功能变化的重要步骤。采用Biolog-FF微孔板培养法,结合土壤理化性质分析,研究黄河三角洲地区不同盐生植被下土壤真菌的碳源代谢活性特征。结果表明,在0~20cm土层,每孔平均吸光值(Average well color development,AWCD值)表现为白茅>罗布麻>柽柳>马绊草>光板地,20~40cm土层则表现为白茅>马绊草>柽柳>罗布麻>光板地,即随盐生植被正向演替,土壤真菌碳源代谢活性显著提高(P<0.05);各样地土壤真菌群落的香农-威尔指数H、丰富度指数S和辛普森指数D按演替顺序均有显著提高,同一样地不同土层中,除马绊草群落,其余样地的3种指数均表现为0~20 cm>20~40cm,说明盐生植被正向演替过程改善了土壤中真菌的多样性和丰富度,提高了群落中常见物种的优势度。土壤理化性质与土壤真菌吸光值、真菌多样性指数相关性分析结果表明,全氮、碱解氮、土壤有机质、磷酸酶和过氧化氢酶显著促进了土壤真菌的碳源代谢活性,土壤含盐量则显著抑制真菌的碳源代谢活性(P<0.05);土壤主成分分析表明,纤维醇、鸟氨酸和D-甘露醇等是0~20 cm土层下土壤真菌利用的主要碳源,D-山梨醇、丙三醇和L-天冬氨酸等则是20~40 cm土层下土壤真菌利用的主要碳源,表明糖类、醇类和酸类是控制土壤真菌碳源代谢发生差异的主要碳源。土壤真菌碳源代谢水平与盐生植被演替关系的结果为黄河三角洲的生态恢复和利用奠定了理论基础。
