Objective： To study the role and mechanism of the Yi medicine, Yi Bu A Jie （以布阿节） extract, in topical treatment of diabetic skin ulcers, with a view to finding a breakthrough natural drug for the prevention and...Objective： To study the role and mechanism of the Yi medicine, Yi Bu A Jie （以布阿节） extract, in topical treatment of diabetic skin ulcers, with a view to finding a breakthrough natural drug for the prevention and treatment of diabetic skin ulcers. Methods： A model of diabetic skin ulcers in Kunming mice was developed. Yi Bu A Jie was extracted in a Soxhlet extractor. Two different concentrations of the extract （0.005 mg/mL and 0.01 mg/mL） were applied to the wound of diabetic skin ulcers once every 3 days, and local skin appearance and histopathological changes were observed. Resalts： The shortest healing time was 25.25 ±2.06 day with a low concentration （P=0.0037 compared with the high concentration group, 33.14＋2.21 day; P=0.0082 compared with control group, 28.21 ± 2.14 days）. The longest healing time was in the high concentration group （P=0.0025 compared with the control group）. In both groups, a large number of inflammatory neutrophil cells were exuded dudng the experimental period. In the low concentration group, capillary-rich granulation tissue and actively growing fibroblasts appeared in the wound, while there was much necrotic tissue in the high concentration group. Conclusion： Yi Bu A Jie extract has an inhibitory effect on diabetic skin ulcers in mice, and the low concentration is more suitable.展开更多
基金Supported by the Project of Shanghai Natural Science Foundation(No.08JC1417000)Research and Innovation Key Project of Shanghai Municipal Education Commission(No. 11ZZ120)
文摘Objective： To study the role and mechanism of the Yi medicine, Yi Bu A Jie （以布阿节） extract, in topical treatment of diabetic skin ulcers, with a view to finding a breakthrough natural drug for the prevention and treatment of diabetic skin ulcers. Methods： A model of diabetic skin ulcers in Kunming mice was developed. Yi Bu A Jie was extracted in a Soxhlet extractor. Two different concentrations of the extract （0.005 mg/mL and 0.01 mg/mL） were applied to the wound of diabetic skin ulcers once every 3 days, and local skin appearance and histopathological changes were observed. Resalts： The shortest healing time was 25.25 ±2.06 day with a low concentration （P=0.0037 compared with the high concentration group, 33.14＋2.21 day; P=0.0082 compared with control group, 28.21 ± 2.14 days）. The longest healing time was in the high concentration group （P=0.0025 compared with the control group）. In both groups, a large number of inflammatory neutrophil cells were exuded dudng the experimental period. In the low concentration group, capillary-rich granulation tissue and actively growing fibroblasts appeared in the wound, while there was much necrotic tissue in the high concentration group. Conclusion： Yi Bu A Jie extract has an inhibitory effect on diabetic skin ulcers in mice, and the low concentration is more suitable.
