Objective To assess the effects of Yi Qi Jie Du Formula(YQJDF)combined with salinomycin(SAL)on nasopharyngeal carcinoma stem cells(NPC-SCs)and investigate the underlying molecular mechanisms.Methods Cell counting meth...Objective To assess the effects of Yi Qi Jie Du Formula(YQJDF)combined with salinomycin(SAL)on nasopharyngeal carcinoma stem cells(NPC-SCs)and investigate the underlying molecular mechanisms.Methods Cell counting methods,the CCK-8 assay,transwell migration assay and JC-1 staining,were used to observe the effects of the combination on the proliferation,migration and apoptosis of NPC stem cells,respectively.Western blot was used to detect the levels of protein in NPC-SCs.Results YQJDF combined with SAL had a synergistic effect on the inhibition of proliferation and migration and induction of NPCSC apoptosis.Mechanistically,YQJDF combined with SAL synergistically upregulated the levels of apoptotic proteins,including cleaved Caspase-3,cleaved Caspase-7 and cleaved Caspase-9.Moreover,YQJDF combined with SAL synergistically decreased the levels of CD44,p-c-Src,Ras,p-PKCδ,p-MEK,p-c-Raf,p-ERK1/2 and p-AKT proteins.Conclusions The combination of YQJDF and SAL has a synergistic effect on the inhibition of NPC-SC proliferation and migration and induction of apoptosis,which may be closely related to the downregulation of the CD44/Ras signaling pathway.展开更多
Objective To assess the effects of Yi Qi Jie Du Decoction(YQJDD)on nasopharyngeal carcinoma stem cells(NPC-SCs)and investigate the underlying mechanism.Methods NPC-SCs were collected in serum-free culture system and i...Objective To assess the effects of Yi Qi Jie Du Decoction(YQJDD)on nasopharyngeal carcinoma stem cells(NPC-SCs)and investigate the underlying mechanism.Methods NPC-SCs were collected in serum-free culture system and identified by the sphere formation assay.The effect of YQJDD on the proliferation of NPC-SCs was detected using the Cell Counting Kit-8 assay.The change of mitochondrial membrane potential(ΔΨm)in NPC-SCs following treatment with YQJDD was investigated by JC-1 staining.The levels of apoptosisassociated proteins were examined using western blot analysis.Results YQJDD significantly inhibited the proliferation of NPCSCs in dose-and time-dependent manners.JC-1 staining revealed that YQJDD lowered theΔΨm of NPC-SCs in a timedependent manner.After treatment with YQJDD,the expression of cleaved caspase-3,-7 and-9,cleaved poly-ADP ribose polymerase,P21 and P53 were increased,while the expression of Survivin in NPC-SCs was decreased.However,the expression of cleaved caspase-8 remained nearly unchanged.Conclusions YQJDD inhibits the growth and induces apoptosis of NPC-SCs via an intrinsic apoptosis pathway.展开更多
目的观察针刺配合通络益气汤治疗脑小血管病致认知功能障碍的临床疗效及其对患者脑微循环、步态平衡和血清神经元PAS结构域蛋白4(neuronal PAS domain protein 4,NPAS4)、P-选择素(CD62P)表达的影响。方法将98例脑小血管病致认知功能障...目的观察针刺配合通络益气汤治疗脑小血管病致认知功能障碍的临床疗效及其对患者脑微循环、步态平衡和血清神经元PAS结构域蛋白4(neuronal PAS domain protein 4,NPAS4)、P-选择素(CD62P)表达的影响。方法将98例脑小血管病致认知功能障碍患者随机分为治疗组和对照组,每组49例。治疗组采用针刺配合通络益气汤治疗,对照组采用单纯通络益气汤治疗。观察两组治疗前后各项实验室指标[谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、晚期糖基化终末产物(advanced glycation end products,AGEs)、NPAS4、CD62P、一氧化氮(NO)、亲环素A(cyclophilin A,CyPA)、脂蛋白相关磷脂酶A2(lipoprotein-associated phospholipase A2,Lp-PLA2)、血管性血友病因子(von Willebrand factor,vFW)水平]、脑微循环指标[血脑屏障通透性、血管差压、临界压力(critical pressure,CP)、脑血管动态阻力(dynamic resistance,DR)]及各量表[Berg平衡量表(Berg balance scale,BBS)、Tinetti平衡与步态量表、蒙特利尔认知评估量表(Montreal cognitive assessment,MoCA]评分的变化情况,并比较两组临床疗效。结果两组治疗后GSH-Px、NO水平及CP、各量表评分均较同组治疗前显著上升,AGEs、NPAS4、CD62P、CyPA、Lp-PLA2、vFW水平及血脑屏障通透性、血管差压、DR显著下降,差异均具有统计学意义(P<0.05)。治疗组治疗后GSH-Px、NO水平及CP、各量表评分均明显高于对照组,AGEs、NPAS4、CD62P、CyPA、Lp-PLA2、vFW水平及血脑屏障通透性、血管差压、DR均明显低于对照组,两组比较差异均具有统计学意义(P<0.05)。治疗组总有效率为98.0%,明显高于对照组的87.8%,两组比较差异具有统计学意义(P<0.05)。结论针刺配合通络益气汤治疗脑小血管病致认知功能障碍疗效确切,可减少氧化应激反应及炎症反应,降低NPAS4、CD62P水平,减少血管内皮功能及认知功能损伤,改善血脑屏障通透性、脑微循环及步态平衡。展开更多
基金We thank for the funding support from the National Natural Science Foundation of China(No.81874408,No.81973914 and No.81573721)the Natural Science Foundation of Hunan Province,China(No.2019JJ40216)Study Fundation of Hunan Provincial Education Department(No.19B439).
文摘Objective To assess the effects of Yi Qi Jie Du Formula(YQJDF)combined with salinomycin(SAL)on nasopharyngeal carcinoma stem cells(NPC-SCs)and investigate the underlying molecular mechanisms.Methods Cell counting methods,the CCK-8 assay,transwell migration assay and JC-1 staining,were used to observe the effects of the combination on the proliferation,migration and apoptosis of NPC stem cells,respectively.Western blot was used to detect the levels of protein in NPC-SCs.Results YQJDF combined with SAL had a synergistic effect on the inhibition of proliferation and migration and induction of NPCSC apoptosis.Mechanistically,YQJDF combined with SAL synergistically upregulated the levels of apoptotic proteins,including cleaved Caspase-3,cleaved Caspase-7 and cleaved Caspase-9.Moreover,YQJDF combined with SAL synergistically decreased the levels of CD44,p-c-Src,Ras,p-PKCδ,p-MEK,p-c-Raf,p-ERK1/2 and p-AKT proteins.Conclusions The combination of YQJDF and SAL has a synergistic effect on the inhibition of NPC-SC proliferation and migration and induction of apoptosis,which may be closely related to the downregulation of the CD44/Ras signaling pathway.
基金funding support from the National Natural Science Foundation of China (No. 81573721, No. 81874408 and No. 81973914)the Natural Science Foundation of Hunan (No. 2017JJ3246 and No. 2019JJ40216)Excellent Youth Fund of Hunan Provincial Education Department (No. 19B439)
文摘Objective To assess the effects of Yi Qi Jie Du Decoction(YQJDD)on nasopharyngeal carcinoma stem cells(NPC-SCs)and investigate the underlying mechanism.Methods NPC-SCs were collected in serum-free culture system and identified by the sphere formation assay.The effect of YQJDD on the proliferation of NPC-SCs was detected using the Cell Counting Kit-8 assay.The change of mitochondrial membrane potential(ΔΨm)in NPC-SCs following treatment with YQJDD was investigated by JC-1 staining.The levels of apoptosisassociated proteins were examined using western blot analysis.Results YQJDD significantly inhibited the proliferation of NPCSCs in dose-and time-dependent manners.JC-1 staining revealed that YQJDD lowered theΔΨm of NPC-SCs in a timedependent manner.After treatment with YQJDD,the expression of cleaved caspase-3,-7 and-9,cleaved poly-ADP ribose polymerase,P21 and P53 were increased,while the expression of Survivin in NPC-SCs was decreased.However,the expression of cleaved caspase-8 remained nearly unchanged.Conclusions YQJDD inhibits the growth and induces apoptosis of NPC-SCs via an intrinsic apoptosis pathway.
