Yi-Qi-Huo-Xue Decoction(YQHX)is the recombination of Dang-Gui-Bu-Xue Decoction(DBD),which is one of the well-known traditional Chinese Medicine(TCM)prescription,and has long been shown to have significant protective e...Yi-Qi-Huo-Xue Decoction(YQHX)is the recombination of Dang-Gui-Bu-Xue Decoction(DBD),which is one of the well-known traditional Chinese Medicine(TCM)prescription,and has long been shown to have significant protective effects against myocardial ischemic injury.In previous studies,we found that YQHX could regulate lipid and glucose metabolism,promote angiogenesis,attenuate inflammatory response,and ameliorate left ventricular function in myocardial ischemia rat models.However,the underlying mechanism of how YQHX involves in lipid metabolism remains unclear so far.In this study,the underlying mechanism of YQHX in lipid metabolism disorders was elucidated in a myocardial ischemia rat model and a hypoxia-induced H9 c2 cell injury model.YQHX(8.2 g·kg-1)and positive-control drug trimetazidine(10 mg·kg-1)were administered daily on the second day after left anterior descending(LAD)operation.At 7 days and 28 days after surgery,changes of cardiac morphology,structure,and function were evaluated by H&E staining and echocardiography,respectively.The plasma lipid levels and mitochondrial ATP content were also evaluated.Western blot and RT-PCR were used to determine the protein and mRNA expressions of AMPK,PGC-1α,CPT-1α,and PPARα.YQHX improved cardiac function and ameliorated lipid metabolism disorders.Furthermore,YQHX increased the expression of p-AMPK,PGC-1α,and CPT-1αwithout changing PPARαin ischemic rat myocardium.In vitro,YQHX activated the protein and mRNA expression of PGC-1α,CPT-1α,and PPARαin hypoxia-induced H9 c2 cells injury,whereas AMPK inhibitor Compound c blocked the effects of YQHX.Taken together,the results suggest that YQHX reduces lipid metabolism disorders in myocardial ischemia via the AMPK-dependent signaling pathway.展开更多
目的:通过观察佛手益气活血汤对血栓闭塞性脉管炎(TAO)模型大鼠血栓素B2(TXB2)、6-酮-前列腺素F1α(6-Keto-PGF1α)、血管内皮生长因子(VEGF)表达的影响,探讨佛手益气活血汤对大鼠TAO的治疗作用及其机制。方法:将Wistar大鼠60只随机分...目的:通过观察佛手益气活血汤对血栓闭塞性脉管炎(TAO)模型大鼠血栓素B2(TXB2)、6-酮-前列腺素F1α(6-Keto-PGF1α)、血管内皮生长因子(VEGF)表达的影响,探讨佛手益气活血汤对大鼠TAO的治疗作用及其机制。方法:将Wistar大鼠60只随机分为假手术组,模型对照组,佛手益气活血汤高、中、低剂量组,每组12只,假手术组及模型对照组大鼠分别灌胃10 m L/kg生理盐水,佛手益气活血汤高、中、低剂量组大鼠分别灌胃佛手益气活血汤30.0、15.0、7.5 g/kg;连续灌胃14天后检测大鼠血浆TXB2、6-Keto-PGF1α及VEGF表达水平。结果:佛手益气活血汤对模型大鼠血浆6-Keto-PGF1α水平具有升高作用,对TXB2及肌肉VEGF表达具有降低作用,与模型对照组比较差异具有统计学意义(P<0.05)。结论:佛手益气活血汤对血栓闭塞性脉管炎模型大鼠病变程度具有一定的改善作用,可能通过调节血浆TXB2及6-K-PGF1α平衡,降低组织VEGF表达而发挥作用。展开更多
目的:探讨益气温阳、活血利水方药对充血性心力衰竭(CHF)大鼠模型胰岛素抵抗(IR)及心肌组织病理学改变的影响。方法:通过对Wistar雄性大鼠尾静脉注射盐酸阿霉素制备充血性心力衰竭大鼠模型,造模成功后将Wistar雄性大鼠随机分成模型组、...目的:探讨益气温阳、活血利水方药对充血性心力衰竭(CHF)大鼠模型胰岛素抵抗(IR)及心肌组织病理学改变的影响。方法:通过对Wistar雄性大鼠尾静脉注射盐酸阿霉素制备充血性心力衰竭大鼠模型,造模成功后将Wistar雄性大鼠随机分成模型组、西药组、方药低剂量组、方药中剂量组、方药高剂量组,连续灌胃4周后,取内眦静脉窦静脉血3 m L,测血糖及血清胰岛素,计算IR。最后处死大鼠,取出心脏,切片HE染色,观察心肌组织病理学改变。结果:与模型组比较,益气温阳、活血利水方药能明显改善CHF大鼠IR水平(P<0.05),其中高、中剂量组较为明显;同时益气温阳、活血利水方药能改善CHF大鼠心肌细胞的损伤,高剂量组心肌细胞形态学的改变接近地高辛组。结论:益气温阳、活血利水方药能一定程度逆转大鼠CHF的IR并改善心室重构。展开更多
基金the National Natural Science Foundation of China(No.81473552)the China Postdoctoral Science Foundation(No.2019TQ0043)。
文摘Yi-Qi-Huo-Xue Decoction(YQHX)is the recombination of Dang-Gui-Bu-Xue Decoction(DBD),which is one of the well-known traditional Chinese Medicine(TCM)prescription,and has long been shown to have significant protective effects against myocardial ischemic injury.In previous studies,we found that YQHX could regulate lipid and glucose metabolism,promote angiogenesis,attenuate inflammatory response,and ameliorate left ventricular function in myocardial ischemia rat models.However,the underlying mechanism of how YQHX involves in lipid metabolism remains unclear so far.In this study,the underlying mechanism of YQHX in lipid metabolism disorders was elucidated in a myocardial ischemia rat model and a hypoxia-induced H9 c2 cell injury model.YQHX(8.2 g·kg-1)and positive-control drug trimetazidine(10 mg·kg-1)were administered daily on the second day after left anterior descending(LAD)operation.At 7 days and 28 days after surgery,changes of cardiac morphology,structure,and function were evaluated by H&E staining and echocardiography,respectively.The plasma lipid levels and mitochondrial ATP content were also evaluated.Western blot and RT-PCR were used to determine the protein and mRNA expressions of AMPK,PGC-1α,CPT-1α,and PPARα.YQHX improved cardiac function and ameliorated lipid metabolism disorders.Furthermore,YQHX increased the expression of p-AMPK,PGC-1α,and CPT-1αwithout changing PPARαin ischemic rat myocardium.In vitro,YQHX activated the protein and mRNA expression of PGC-1α,CPT-1α,and PPARαin hypoxia-induced H9 c2 cells injury,whereas AMPK inhibitor Compound c blocked the effects of YQHX.Taken together,the results suggest that YQHX reduces lipid metabolism disorders in myocardial ischemia via the AMPK-dependent signaling pathway.
文摘目的:通过观察佛手益气活血汤对血栓闭塞性脉管炎(TAO)模型大鼠血栓素B2(TXB2)、6-酮-前列腺素F1α(6-Keto-PGF1α)、血管内皮生长因子(VEGF)表达的影响,探讨佛手益气活血汤对大鼠TAO的治疗作用及其机制。方法:将Wistar大鼠60只随机分为假手术组,模型对照组,佛手益气活血汤高、中、低剂量组,每组12只,假手术组及模型对照组大鼠分别灌胃10 m L/kg生理盐水,佛手益气活血汤高、中、低剂量组大鼠分别灌胃佛手益气活血汤30.0、15.0、7.5 g/kg;连续灌胃14天后检测大鼠血浆TXB2、6-Keto-PGF1α及VEGF表达水平。结果:佛手益气活血汤对模型大鼠血浆6-Keto-PGF1α水平具有升高作用,对TXB2及肌肉VEGF表达具有降低作用,与模型对照组比较差异具有统计学意义(P<0.05)。结论:佛手益气活血汤对血栓闭塞性脉管炎模型大鼠病变程度具有一定的改善作用,可能通过调节血浆TXB2及6-K-PGF1α平衡,降低组织VEGF表达而发挥作用。
文摘目的:探讨益气温阳、活血利水方药对充血性心力衰竭(CHF)大鼠模型胰岛素抵抗(IR)及心肌组织病理学改变的影响。方法:通过对Wistar雄性大鼠尾静脉注射盐酸阿霉素制备充血性心力衰竭大鼠模型,造模成功后将Wistar雄性大鼠随机分成模型组、西药组、方药低剂量组、方药中剂量组、方药高剂量组,连续灌胃4周后,取内眦静脉窦静脉血3 m L,测血糖及血清胰岛素,计算IR。最后处死大鼠,取出心脏,切片HE染色,观察心肌组织病理学改变。结果:与模型组比较,益气温阳、活血利水方药能明显改善CHF大鼠IR水平(P<0.05),其中高、中剂量组较为明显;同时益气温阳、活血利水方药能改善CHF大鼠心肌细胞的损伤,高剂量组心肌细胞形态学的改变接近地高辛组。结论:益气温阳、活血利水方药能一定程度逆转大鼠CHF的IR并改善心室重构。
