The calculation theories and analysis methods of thermodynamic stability of embankment engineering in cold regions are systematically summarized.The engineering theories and methods taken to control frost heave and th...The calculation theories and analysis methods of thermodynamic stability of embankment engineering in cold regions are systematically summarized.The engineering theories and methods taken to control frost heave and thaw settlement in seasonal frozen soil regions may not be applicable in permafrost regions.The active cooling technology of roadbed should be utilized to limit the effects caused by both climate changes and human engineering activities.The paper mainly discussed the calculation theories and analysis methods of four kinds of embankment structures,i.e.,the crushed-rock embankment,duct-ventilated embankment,thermosyphon embankment,and composite embankment.It is expected that a scientific basis could be provided for the theory,design,and application of embankment constructions in cold regions.展开更多
以甜菜红苷(Bt)为对象,采用-荧光光谱、傅里叶变换红外光谱以及圆二色谱技术分析乳清蛋白(WP)和牛血清白蛋白(BSA)与Bt作用生成的复合物中乳蛋白组分对Bt热稳定性的影响。结果表明:添加WP和BSA能有效抑制甜菜红苷的热降解作用,其中WP对...以甜菜红苷(Bt)为对象,采用-荧光光谱、傅里叶变换红外光谱以及圆二色谱技术分析乳清蛋白(WP)和牛血清白蛋白(BSA)与Bt作用生成的复合物中乳蛋白组分对Bt热稳定性的影响。结果表明:添加WP和BSA能有效抑制甜菜红苷的热降解作用,其中WP对甜菜红苷的保护作用优于BSA。同时,体系环境的pH值变化也明显影响甜菜红苷的热降解,在p H 5.0时甜菜红苷复合物的半衰期最长,其中Bt-WP复合物的半衰期达2.99 h。荧光光谱、傅里叶变换红外光谱以及圆二色谱分析表明,复合物中Bt组分的作用导致WP和BSA发生静态荧光猝灭,WP和BSA色氨酸残基和酪氨酸残基所处微环境的疏水性减弱,极性变强。此外,添加Bt使两种蛋白质的构象结构发生改变,酰胺Ⅰ带和酰胺Ⅱ带的特征峰位置改变,二级结构中各类构象组成发生不同程度的变化。展开更多
基金supported by the Western Project Program of the Chinese Academy of Sciences(KZCX2-XB3-19)the National Natural Science Foundation of China(41230630)+2 种基金the National Basic Research Program of China(2012CB026102)the Knowledge Innovation Program of the Chinese Academy of Sciences(KZCX2-EW-QN301)the Project for Incubation of Specialists inGlaciology and Geocryology of the National Natural Science Foundation of China(J1210003/J0109)
文摘The calculation theories and analysis methods of thermodynamic stability of embankment engineering in cold regions are systematically summarized.The engineering theories and methods taken to control frost heave and thaw settlement in seasonal frozen soil regions may not be applicable in permafrost regions.The active cooling technology of roadbed should be utilized to limit the effects caused by both climate changes and human engineering activities.The paper mainly discussed the calculation theories and analysis methods of four kinds of embankment structures,i.e.,the crushed-rock embankment,duct-ventilated embankment,thermosyphon embankment,and composite embankment.It is expected that a scientific basis could be provided for the theory,design,and application of embankment constructions in cold regions.
文摘以甜菜红苷(Bt)为对象,采用-荧光光谱、傅里叶变换红外光谱以及圆二色谱技术分析乳清蛋白(WP)和牛血清白蛋白(BSA)与Bt作用生成的复合物中乳蛋白组分对Bt热稳定性的影响。结果表明:添加WP和BSA能有效抑制甜菜红苷的热降解作用,其中WP对甜菜红苷的保护作用优于BSA。同时,体系环境的pH值变化也明显影响甜菜红苷的热降解,在p H 5.0时甜菜红苷复合物的半衰期最长,其中Bt-WP复合物的半衰期达2.99 h。荧光光谱、傅里叶变换红外光谱以及圆二色谱分析表明,复合物中Bt组分的作用导致WP和BSA发生静态荧光猝灭,WP和BSA色氨酸残基和酪氨酸残基所处微环境的疏水性减弱,极性变强。此外,添加Bt使两种蛋白质的构象结构发生改变,酰胺Ⅰ带和酰胺Ⅱ带的特征峰位置改变,二级结构中各类构象组成发生不同程度的变化。
