Objective:To explore the molecular mechanism of buzhong yiqi decoction in the treatment of senile functional constipation based on network pharmacology.Methods:Chemical compositions and selected targets related to the...Objective:To explore the molecular mechanism of buzhong yiqi decoction in the treatment of senile functional constipation based on network pharmacology.Methods:Chemical compositions and selected targets related to the eight traditional Chinese medicine herbs were searched through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP).Through GeneCards database and OMIM database,disease targets of senile functional constipation were searched.R language was used to screen the common targets between drugs and disease,and then the interaction network diagram of the targets was constructed by String.Cytoscape3.7.0 was applied to construct the traditional Chinese medicines-chemical compositions-disease targets network.GO enrichment analysis and KEGG enrichment analysis of the targets were based on DAVID.Results:Five main chemical compositions including quercetin,kaempferol,nobiletin,naringenin and formononetin were screened,and five key targets including PTGS2,ESR1,AR,NOS2 and PPARG were identified.622 GO functional entries and 125 pathways were yielded by gene enrichment analysis.Conclusion:Buzhong yiqi decoction may play the role of anti-inflammatory and anti-oxidation through multi-component,multi-target and multi-channel,so as to improve senile functional constipation.展开更多
Objective： To investigate the influence of Yiqi Huatan Decoction （益气化痰方, YHD） on a model of depression in rats under different pathological conditions. Methods： Thirty-two male SD rats were randomly divided i...Objective： To investigate the influence of Yiqi Huatan Decoction （益气化痰方, YHD） on a model of depression in rats under different pathological conditions. Methods： Thirty-two male SD rats were randomly divided into 4 groups of 8： normal, model, YHD, and maprotiline. The model group, YHD group and maprotiline group used separate feeding and rats were exposed to chronic and unpredictable stress to build the depression model. From day 2, the YHD group and maprotiline group were respectively given YHD （7 g/kg） and maprotiline （10 mg/kg） by gastrogavage once daily. The normal and model groups were given the same volume of drinking water. The medication duration were 21 days. At the end of the experiment, the serum levels of copper and zinc were determined by atomic absorption spectroscopy, plasma concentrations of adrenocorticotropic hormone （ACTH） and cortisol （COR） were detected by radioimmunoassay, and levels of norepinephrine （NE）, dopamine （DA）, and 5-hydroxytryptamine （5-HT） in the hypothalamus were analysed by high performance liquid chromatography-eletricochemistry. Results： Compared with the content of copper and zinc in the serum of rats in the normal group, serum copper levels in model rats were significantly increased and zinc content was significantly reduced （both P〈0.05）. Plasma concentrations of ACTH and COR in the model group were significantly increased compared with those in the normal group （P〈0.05, P〈0.01）. The contents of NE, DA, and 5-HT in the hypothalamus of rats in the model group were significantly reduced compared with those of the normal group （P〈0.05 or P〈0.01）. Compared with those in the model group, the serum copper content and plasma concentrations of ACTH and COR were significantly decreased （all P〈0.05）; meanwhile, serum zinc content and hypothalamic contents of NE, DA, and 5-HT were significantly increased in rats of the YHD group （all P〈0.05）. The same effects were also shown in the maprotiline group except for 5-HT （all P〈0.05）. Conclusion： The pharmacological actions of YHD for depression might be related to improving trace-element anomalies, reversing endocrine dysfunction, and modulating the disorders of monoaminergic neurotransmitters.展开更多
OBJECTIVE:To explored the mechanism of Buzhong Yigi decoction(补中益气汤 BZYQD) in inhibiting prostatic cell proliferation effect.METHODS:The compounds of BZYQD consisted with eight herbs were searched in TCMSP databa...OBJECTIVE:To explored the mechanism of Buzhong Yigi decoction(补中益气汤 BZYQD) in inhibiting prostatic cell proliferation effect.METHODS:The compounds of BZYQD consisted with eight herbs were searched in TCMSP databases and the putative targets of BZYQD were collected in Drugbank database.Then,“Benign prostatic hyperplasia”(BPH) was used to find the targets based on the Gene Cards,Online Mendelian Inheritance in Man(OMIM) and Therapeutic Target Database(TTD) databases,and they were further used to collect further collect the intersection targets between BZYQD and BPH by counter-selection.Next,Herb-Compound-Target-Disease network was constructed by Cytoscape software and protein interaction network was built by Search tool for recurring instances of neighbouring genes(STRING) database.Gene Ontology(GO) enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment were analyzed by Database for Annotation,Visualization and Integrated Discovery(DAVID) database to predict the mechanism of the intersection targets.Mitogen activated protein kinase 8(MAPK8),interleukin 6(IL-6) and quercetin were chosen to perform molecular docking.Then 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-Htetrazolium bromide(MTT) assay was to detect the via bility of BPH-1(BPH epithelial cell line) by treated with quercetin at the concentrations of 15,30,60,120 μM for 12,24,48,72 h.The production of IL-6,tumor necrosis factor-α(TNF-α),IL-1β and were m RNA expression detected by enzyme-linked immunosorbent assay kit and quantitative real-time polymerase chain reaction.Western blot was used to detect the expression of phospho-p38 mitogen-activated protein kinase(p-P38) and matrix metalloprotein-9(MMP-9).RESULTS:A total 151 chemical ingredients of 8 herbs and 1756 targets in BZYQD,105 common targets of BZYQD and BPH which mainly involving with MAPK8,IL-6,and so on.GO enrichment analysis got 352 GO entries(P < 0.05) which included 208 entries of biological process,64 entries of cell component and 80 entries of molecular function.KEGG pathway Enrichment analyses got 20 significant pathways which mainly involved with MAPK signaling way.MTT assay indicated quercetin inhibited the viability of BPH-1 cells by time-and dosedependent manner.Quercetin decreased the IL-6,TNF-α and IL-1β production and m RNA expression,and the expression of p-P38 and MMP-9 were also obviously reduced after treated with quercetin.CONCLUSIONS:BZYQD inhibited BPH through suppressing inflammatory response which might involving with regulating the MAPK signaling way.展开更多
基金Construction project of studio for reputed physicians of TCM of 13th Five-years planning in Nanjing(No.ZXP-2019-NJ)Scientific research project of health care for cadres of Jiangsu province in 2018(No.BJ18001)
文摘Objective:To explore the molecular mechanism of buzhong yiqi decoction in the treatment of senile functional constipation based on network pharmacology.Methods:Chemical compositions and selected targets related to the eight traditional Chinese medicine herbs were searched through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP).Through GeneCards database and OMIM database,disease targets of senile functional constipation were searched.R language was used to screen the common targets between drugs and disease,and then the interaction network diagram of the targets was constructed by String.Cytoscape3.7.0 was applied to construct the traditional Chinese medicines-chemical compositions-disease targets network.GO enrichment analysis and KEGG enrichment analysis of the targets were based on DAVID.Results:Five main chemical compositions including quercetin,kaempferol,nobiletin,naringenin and formononetin were screened,and five key targets including PTGS2,ESR1,AR,NOS2 and PPARG were identified.622 GO functional entries and 125 pathways were yielded by gene enrichment analysis.Conclusion:Buzhong yiqi decoction may play the role of anti-inflammatory and anti-oxidation through multi-component,multi-target and multi-channel,so as to improve senile functional constipation.
基金Supported by the Science Foundation of Fujian Province(No.2010J01239)the Xiamen Science and Technology Key Program Grant(No.3502Z20100006)Hong Kong Healthy Walker Co.,Ltd(No.XDTH2009023A)
文摘Objective： To investigate the influence of Yiqi Huatan Decoction （益气化痰方, YHD） on a model of depression in rats under different pathological conditions. Methods： Thirty-two male SD rats were randomly divided into 4 groups of 8： normal, model, YHD, and maprotiline. The model group, YHD group and maprotiline group used separate feeding and rats were exposed to chronic and unpredictable stress to build the depression model. From day 2, the YHD group and maprotiline group were respectively given YHD （7 g/kg） and maprotiline （10 mg/kg） by gastrogavage once daily. The normal and model groups were given the same volume of drinking water. The medication duration were 21 days. At the end of the experiment, the serum levels of copper and zinc were determined by atomic absorption spectroscopy, plasma concentrations of adrenocorticotropic hormone （ACTH） and cortisol （COR） were detected by radioimmunoassay, and levels of norepinephrine （NE）, dopamine （DA）, and 5-hydroxytryptamine （5-HT） in the hypothalamus were analysed by high performance liquid chromatography-eletricochemistry. Results： Compared with the content of copper and zinc in the serum of rats in the normal group, serum copper levels in model rats were significantly increased and zinc content was significantly reduced （both P〈0.05）. Plasma concentrations of ACTH and COR in the model group were significantly increased compared with those in the normal group （P〈0.05, P〈0.01）. The contents of NE, DA, and 5-HT in the hypothalamus of rats in the model group were significantly reduced compared with those of the normal group （P〈0.05 or P〈0.01）. Compared with those in the model group, the serum copper content and plasma concentrations of ACTH and COR were significantly decreased （all P〈0.05）; meanwhile, serum zinc content and hypothalamic contents of NE, DA, and 5-HT were significantly increased in rats of the YHD group （all P〈0.05）. The same effects were also shown in the maprotiline group except for 5-HT （all P〈0.05）. Conclusion： The pharmacological actions of YHD for depression might be related to improving trace-element anomalies, reversing endocrine dysfunction, and modulating the disorders of monoaminergic neurotransmitters.
文摘OBJECTIVE:To explored the mechanism of Buzhong Yigi decoction(补中益气汤 BZYQD) in inhibiting prostatic cell proliferation effect.METHODS:The compounds of BZYQD consisted with eight herbs were searched in TCMSP databases and the putative targets of BZYQD were collected in Drugbank database.Then,“Benign prostatic hyperplasia”(BPH) was used to find the targets based on the Gene Cards,Online Mendelian Inheritance in Man(OMIM) and Therapeutic Target Database(TTD) databases,and they were further used to collect further collect the intersection targets between BZYQD and BPH by counter-selection.Next,Herb-Compound-Target-Disease network was constructed by Cytoscape software and protein interaction network was built by Search tool for recurring instances of neighbouring genes(STRING) database.Gene Ontology(GO) enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment were analyzed by Database for Annotation,Visualization and Integrated Discovery(DAVID) database to predict the mechanism of the intersection targets.Mitogen activated protein kinase 8(MAPK8),interleukin 6(IL-6) and quercetin were chosen to perform molecular docking.Then 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-Htetrazolium bromide(MTT) assay was to detect the via bility of BPH-1(BPH epithelial cell line) by treated with quercetin at the concentrations of 15,30,60,120 μM for 12,24,48,72 h.The production of IL-6,tumor necrosis factor-α(TNF-α),IL-1β and were m RNA expression detected by enzyme-linked immunosorbent assay kit and quantitative real-time polymerase chain reaction.Western blot was used to detect the expression of phospho-p38 mitogen-activated protein kinase(p-P38) and matrix metalloprotein-9(MMP-9).RESULTS:A total 151 chemical ingredients of 8 herbs and 1756 targets in BZYQD,105 common targets of BZYQD and BPH which mainly involving with MAPK8,IL-6,and so on.GO enrichment analysis got 352 GO entries(P < 0.05) which included 208 entries of biological process,64 entries of cell component and 80 entries of molecular function.KEGG pathway Enrichment analyses got 20 significant pathways which mainly involved with MAPK signaling way.MTT assay indicated quercetin inhibited the viability of BPH-1 cells by time-and dosedependent manner.Quercetin decreased the IL-6,TNF-α and IL-1β production and m RNA expression,and the expression of p-P38 and MMP-9 were also obviously reduced after treated with quercetin.CONCLUSIONS:BZYQD inhibited BPH through suppressing inflammatory response which might involving with regulating the MAPK signaling way.
