INHIBITION OF PROLIFERATION OF HUMAN BREAST CANCER MCF-7 CELLS BY SMALL INTERFERENCE RNA AGAINST LRP16 GENE

Objective: Our previous studies have firstly demonstrated that 17β -E2 up-regulates LRP16 gene expression in human breast cancer MCF-7 cells, and ectopic expression of the LRP16 gene promotes MCF-7 cells proliferation. Here, the effects of the LRP16 gene expression on growth of MCF-7 human breast cancer cells and the mechanism were further studied by establishing two stably LRP16-inhibitory MCR-7 cell lines. Methods: Hairpin small interference RNA (siRNA) strategy, by which hairpin siRNA was released by U6 promoter and was mediated by pLPC-based retroviral vector, was adopted to knockdown endogenous LRP16 level in MCF-7 cells. And the hairpin siRNA against green fluorescence protein (GFP) was used as the negative control. The suppressant efficiency of the LRP16 gene expression was confirmed by Nothern blot. Cell proliferation assay and soft agar colony formation assay were used to determine the status of the cells proliferation. Cell cycle checkpoints including cyclin E and cyclin D1 were examined by Western blot. Results: The results from cell proliferation assays suggested that down-regulation of LRP16 gene expression is capable of inhibiting MCF-7 breast cancer cell growth and down-regulation of the LRP16 gene expression is able to inhibit anchorage-independent growth of breast cancer cells in soft agar. We also demonstrated that cyclin E and cyclin D1 proteins were much lower in the LRP16-inhibitory cells than in the control cells. Conclusion: These data suggest that LRP16 gene play an important role in MCF-7 cells proliferation by regulating the pathway of the G1/S transition and may function as an important modulator in regulating the process of tumorigenesis in human breast.

F-interference law generation and its feature recognition

By using two-directional S-rough sets, the concepts of （f, f）-interference generation and （f, f）- interference law generation of knowledge, F-interference generation and F-interference law generation of two-directional S-rough sets are proposed. Based on the concepts above, the relation theoreras between F-interference loss and F-interference degree, the relation theorems between F-interference loss law and F-interference degree law, the dis- cernibility theorems between F-interference and F-interference law are presented. At last, the recognition criterion of F-interference law and its application are given.

Second-order interference of two independent and tunable single-mode continuous-wave lasers

The second-order temporal interference of two independent single-mode continuous-wave lasers is discussed by em- ploying two-photon interference in Feynman＇s path integral theory. It is concluded that whether the second-order temporal interference pattern can or cannot be retrieved via two-photon coincidence counting rate is dependent on the resolution time of the detection system and the frequency difference between these two lasers. Two identical and tunable single-mode continuous-wave diode lasers are employed to verify the predictions. These studies are helpful to understand the physics of two-photon interference with photons of different spectra.

50 Gbit/s前传光模块关键技术研究与应用展望

随着全球5G网络的规模化商用及移动互联网流量的迅猛增长,更为丰富的无线频谱资源得以释放,高速率光模块在无线前传网络中的需求日益凸显。聚焦于50 Gbit/s前传光模块的关键技术,深入剖析了前传网络的两种主要组网方式,分析了前传链路的典型传输距离及链路损耗情况,探讨了10 km场景下粗波分复用(coarse wavelength division multiplex,CWDM)中各波长的最大色散值和最小色散值,并研究了相应的色散补偿策略,通过实验验证了不同多径干扰(multi-path interference,MPI)强度下系统光功率代价的变化,详细分析了多径干扰噪声对系统误码性能的潜在影响,为前传光模块参数的优化提供了重要的理论依据。此外,深入研究了50 Gbit/s前传光模块的光电芯片技术方案,对多种不同类型光模块的参数性能进行了严格的测试,提出了针对50 Gbit/s前传光模块光电接口参数的标准化建议,详细介绍了当前50 Gbit/s前传光模块在国内外的标准现状,并展望了未来应用的发展趋势。

Interference Experiment with a Transparent Mask Rejects Wave Models of Light

Young’s double-slit experiment shows characteristics of light that are modeled as indicating a wave nature of light. Other experiments suggest a particle model of light. An experiment is performed with the Fraunhofer pattern from a first mask impinging on a transparent second mask with a slit. The screen pattern is an interference pattern such as produced in Young’s Experiment. An opaque strip between the first and second mask blocks the light of the center maxima from the first mask. The screen interference fringes remained. Moving the glass mask so the slit is removed from light shows the necessity of a slit in the second mask. This suggests a Newtonian type of light model. This experiment rejects the wave models of light.

Second-order temporal interference of two independent light beams at an asymmetrical beam splitter

The second-order temporal interference of classical and nonclassical light at an asymmetrical beam splitter is discussed based on two-photon interference in Feynman＇s path integral theory. The visibility of the second-order interference pattern is determined by the properties of the superposed light beams, the ratio between the intensities of these two light beams, and the reflectivity of the asymmetrical beam splitter. Some requirements about the asymmetrical beam splitter have to be satisfied in order to ensure that the visibility of the second-order interference pattern of nonclassical light beams exceeds the classical limit. The visibility of the second-order interference pattern of photons emitted by two independent single-photon sources is independent of the ratio between the intensities. These conclusions are important for the researches and applications in quantum optics and quantum information when an asymmetrical beam splitter is employed.

S-rough Laws and Its Dependent Separation by F-Interference

By employing function S-rough sets,the concepts of f-interference generation and separation of law,and the F-interference generation and separation of S-rough laws were proposed. Base on the concepts above,the F-interference relation theorems,the F-interference discernibility theorem,the dependent separation theorem by interfereence,and the dependent separation principle by interference were presented; finally the application was given. By employing F-interference dependence and separation of S-rough laws,the changes of characteristic of dynamic systemic regularities could be recognized.

Failure to Recover from Proactive Semantic Interference Differentiates Amnestic Mild Cognitive Impairment and PreMCI from Normal Aging after Adjusting for Initial Learning Ability

Background: There is increasing evidence that the failure to recover from proactive semantic interference (frPSI) may be an early cognitive marker of preclinical Alzheimer’s disease (AD). However, it is unclear whether frPSI effects reflect deficiencies in an individual’s initial learning capacity versus the actual inability to learn new semantically related targets. Objective: The current study was designed to adjust for learning capacity and then to examine the extent to which frPSI, proactive semantic interference (PSI) and retroactive semantic interference (RSI) effects could differentiate between older adults who were cognitively normal (CN), and those diagnosed with either Pre-Mild Cognitive Impairment (PreMCI) or amnestic MCI (aMCI). Methods: We employed the LASSI-L cognitive stress test to examine frPSI, PSI and RSI effects while simultaneously controlling for the participant’s initial learning capacity among 50 CN, 35 aMCI, and 16 PreMCI participants who received an extensive diagnostic work-up. Results: aMCI and PreMCI participants showed greater frPSI deficits (50% and 43.8% respectively) compared to only 14% of CNparticipants. PSI effects were observed for aMCI but not PreMCI participants relative to their CN counterparts. RSI failed to differentiate between any of the study groups. Conclusion: By using participants as their own controls and adjusting for overall learning and memory, it is clear that frPSI deficits occur with much greater frequency in individuals at higher risk for Alzheimer’s disease (AD), and likely reflect a failure of brain compensatory mechanisms.

Interference Mitigation in Satellite Personal Communication Networks Using Adaptive Antenna Arrays and Filtering Technique

We address the problem of interference as related to Satellite Personal Communication Networks (S-PCNs). Basic low Earth orbit (LEO) constellation is considered. The paper uses combined adaptive antenna arrays and adaptive filtering technique. This hybrid linear adaptive technique provides improved performance eliminating interference, particularly uncorrelated signals residing in the antenna sidelobes.

Kimberly S.Young关于网络成瘾的15年研究述评

美国匹兹堡大学的Kimberly S.Young作为系统研究网瘾的先驱之一,她对于人类网络成瘾奥秘的追问与探究在过去的15年(1996-2011)里从未间断。本文追踪评述了Young对于网络成瘾的界定与分类,关于网络成瘾产生机理的研究,两度开发的网络成瘾诊断问卷和治疗网瘾的认知行为疗法。

Wagnild-Young心理弹性量表(RS-11)中文版的信效度检验

目的对Wagnild-Young心理弹性量表(RS-11)进行初步的修订和信效度检验。方法根据量表修订的方法,选取某大学在校生3363名进行测试,分析中文版的信、效度。结果项目分析结果表明RS-11各项目的项目鉴别力良好(r=0.344～0.700);内部一致性系数为0.807,分半信度为0.865,量表的重测信度为0.617;与CD-RISC-25关联效度为0.725;与大五人格和SCL-90关联效度理想。结论修订的Wagnild-Young心理弹性量表(RS-11)具有较高的信度和效度。

关于Young’s不等式证明的一个注记

给出Youngs不等式的新证明和一个注记.

多晶Cu中Young's模量和硬度与晶体取向的关系

采用纳米压入(nanoindentation)和电子背散射衍射(EBSD)技术对多晶Cu样品多个晶粒进行了微观力学性能表征和晶体取向分析.结果表明,Young's模量随晶粒表面法向方向<hkl>与<111>和<001>最小夹角的变化有明显的规律:<hkl>越接近<111>,其Young's模量越大;<hkl>越接近<001>,其Young's模量越小;而Young's模量随<hkl>与<110>最小夹角的变化无明显规律.硬度随<hkl>与<111>,<110>和<001>最小夹角的变化均无明显规律.通过理论计算讨论了上述规律性.

A method to determine Young's modulus of soft gels for cell adhesion

A convenient technique is reported in this note for measuring elastic modulus of extremely soft material for cellular adhesion. Specimens of bending cylinder under gravity are used to avoid contact problem between testing device and sample, and a beam model is presented for evaluating the curvatures of gel beams with large elastic deformation. A self-adaptive algorithm is also proposed to search for the best estimation of gels＇ elastic moduli by comparing the experimental bending curvatures with those computed from the beam model with preestimated moduli. Application to the measurement of the property of polyacrylamide gels indi- cates that the material compliance varies with the concentrations of bis-acrylamide, and the gels become softer after being immersed in a culture medium for a period of time, no matter to what extent they are polymerized.

Prediction and optimization of process variables to maximize the Young’s modulus of plasma sprayed alumina coatings on AZ31B magnesium alloy

Like other manufacturing techniques,plasma spraying has also a non-linear behavior because of the contribution of many coating variables.This characteristic results in finding optimal factor combination difficult.Subsequently,the issue can be solved through effective and strategic statistical procedures integrated with systematic experimental data.Plasma spray parameters such as power,stand-off distance and powder feed rate have significant influence on coating characteristics like Young’s modulus.This paper presents the use of statistical techniques in specifically response surface methodology(RSM),analysis of variance,and regression analysis to develop empirical relationship to predict Young’s modulus of plasma-sprayed alumina coatings.The developed empirical relationships can be effectively used to predict Young’s modulus of plasma-sprayed alumina coatings at 95%confidence level.Response graphs and contour plots were constructed to identify the optimum plasma spray parameters to attain maximum Young’s modulus in alumina coatings.A linear regression relationship was established between porosity and Young’s modulus of the alumina coatings.

导叶翼型优化对水泵水轮机“S”特性的影响

为了改善水泵水轮机“S”特性,本文以某水泵水轮机模型机为研究对象,采用SST k-ω湍流模型开展水泵水轮机全流道三维数值计算。通过优化活动导叶翼型,得出n 11-Q 11模拟曲线和机组“S”特性改善情况,并对优化活动导叶翼型后的机组的无叶区进行了压力脉动分析。结果表明:优化活动导叶翼型对机组的“S”特性存在一定改善效果;翼型优化后机组的效率虽然有小幅度下降,但是整体效率依然保持在90%以上;无叶区压力脉动主要是由活动导叶区域出流,转轮叶片工作面发生冲击导致的分流以及转轮叶片与活动导叶组成的动静叶栅干涉效应引起的。改型后的活动导叶降低了无叶区的压力脉动幅值,在一定程度上提升了水泵水轮机的安全系数。

Experimental Study on Young′s Modulus of Concrete

Young′s Modulus of concrete is studied on the basis of triaxial compressive experiments. The authors proposed two empirical equations to calculate its static Young′s modulus and dynamic Young′s modulus when dynamic Poisson ratio μ d varies nearby 0.20.P wave velocity and elastic modulus have the same varying tendency as letter N. μ,μ d decrease with the increase of loading rate and μ d has a great effect on the parameters E d and E D.

Approximate expression of Young's equation and molecular dynamics simulation for its applicability

In 1805, Thomas Young was the first to propose an equation(Young's equation) to predict the value of the equilibrium contact angle of a liquid on a solid. On the basis of our predecessors, we further clarify that the contact angle in Young's equation refers to the super-nano contact angle. Whether the equation is applicable to nanoscale systems remains an open question. Zhu et al. [College Phys. 4 7(1985)] obtained the most simple and convenient approximate formula, known as the Zhu–Qian approximate formula of Young's equation. Here, using molecular dynamics simulation, we test its applicability for nanodrops. Molecular dynamics simulations are performed on argon liquid cylinders placed on a solid surface under a temperature of 90 K, using Lennard–Jones potentials for the interaction between liquid molecules and between a liquid molecule and a solid molecule with the variable coefficient of strength a. Eight values of a between 0.650 and 0.825 are used. By comparison of the super-nano contact angles obtained from molecular dynamics simulation and the Zhu–Qian approximate formula of Young's equation, we find that it is qualitatively applicable for nanoscale systems.

ELECTRIC FIELD EFFECTS ON YOUNG'S MOLULUS OF NANOWIRES

As an important component of nanodevices and nanomachine constructions, the mechanical performance of nanowires （NWs） has been a subject of intense research efforts due to gaining relevance in controlling functionality of nanoelectromechanical systems （NEMS）; meanwhile, one of the characteristics of the NEMS is the dependence of the functionality of the systems upon the applied electric field. The study of the electric effects on the Young＇s modulus of nanostructures is of certain usefulness in the design of NEMS and the precise measurement of mechanical properties of one-dimensional nanostructures. This paper reviews the origin of the size-dependence of the elastic property of NWs and the factors influencing the discrepancies and inconsistencies in the measured values of the Young＇s modulus for the NW, besides the surface effects, nonlinear effects, the electromechanical coupling effects as a possible effect responsible for the differences in quantitative and qualitative performance of the measured Young＇s modulus for the NWs versus the diameter are clarified.

CALCULATION OF THE YOUNG'S MODULUS OF AN ADSORBED POLYMER LAYER

Polymer layers adsorbed to a surface or in a confined environment often change their mechanical properties. There is even the possibility of solidification of the confined layer. To judge the stiffness of such a layer, we used the Hertz model to calculate the Young＇s modulus of the polymer layer in the confinement of AFM experiments with silicon nitride tip with a radius of curvature ofR ≈ 50 nm and a glass sphere attached to the cantilever R =5 μm. Since there is no visible indentation of the layer in the AFM experiments, the layer is either penetrated very easily, or the indentation is too small to be seen in a force curve. The latter would be the case for a polymer layer with a Young＇s modulus above 4 × 10^8 Pa in case of an experiment with a silicon nitride tip and 4×10^5 Pa in case of a glass sphere.