Studies on the Fingerprint of Yubaizhi by High-Performance Liquid Chromato Graphy

The investigation of fingerprint of Yubaizhi was performed by high-performance liquid chromatography （HPLC）. Chromatographic conditions were： the mobile phase was the mixture of acetonitrile-water （43：57, v/v）; the flow rate was 1 mL/min; the column temperature was 40℃ ; the detection wavelength was 254 nm. The stability, precision and reproducibility were performed according to ＂Specification of Fingerprint of Injection of Chinese Traditional Medicine （temporarily）＂ regulated by State Food and Drug Administration（SFDA）. The HPLC fingerprints of Yubaizhi from various locations were well constant.

禹白芷中香豆素成分的HPLC-MS/MS分析

The main constituents of the extract from Yubaizi (the roots of Angelica dahurica)were analyzed by HPLC-MS/MS method,and a total of six cumarins were unequivocally determined.The fragmentation patterns of the cumarins were found useful for their characterization,and the relative contents of the six compounds were also calculated.

禹白芷根脂溶性化学成分研究

该文研究禹白芷Angelica dahurica cv.Yubaizhi根脂溶性化学成分。采用硅胶和高效液相等柱色谱方法进行分离纯化,通过化合物的谱学数据鉴定其结构。共分离得到33个化合物,分别鉴定为异欧前胡素(1)、欧前胡素(2)、豆甾醇(3)、异氧化前胡内酯(4)、栓翅芹烯醇(5)、补骨脂素(6)、香柑内酯(7)、异去甲基呋喃羽叶芸香素(8)、珊瑚菜内酯(9)、欧芹酚(10)、别欧前胡素(11)、花椒毒素(12)、花椒毒酚(13)、异茴芹内酯(14)、别异欧前胡素(15)、β-谷甾醇(16)、氧化别欧前胡素(17)、牧草栓翅芹酮(18)、5-羟基-8-甲氧基补骨脂素(19)、二氢欧山芹醇(20)、独活醇(21)、异栓翅芹烯醇(22)、2"R-新白当归脑(23)、白当归素乙醚(24)、白当归素(25)、水合氧化前胡内酯(26)、尿嘧啶(27)、伞形花内酯(28)、佛手酚(29)、去甲基呋喃羽叶芸香素(30)、异白当归脑(31)、氧化前胡内酯乙醇醚(32)和独活属醇(33)。其中,化合物8,10,17,21和30为首次从该种植物中分离得到。

禹白芷香豆素成分在人源Caco-2细胞单层模型上的吸收转运研究

吸收是口服药物发挥疗效的关键,小肠是药物吸收的主要场所。该文采用国际公认的可以模拟小肠上皮细胞的人结肠腺癌细胞系Caco-2细胞单层模型,分别用6孔和12孔Transwell板,对禹白芷总香豆素及主要香豆素单体化合物进行了肠吸收转运对比研究,探索禹白芷中香豆素成分在香豆素单体给药及提取物给药情况下,吸收转运是否存在差异。该文研究发现水合氧化前胡内酯、比克白芷素和珊瑚菜内酯单体单独给药,其表观渗透系数(P_(app))皆在1×10^(-5) cm·s^(-1)数量级,判断均为吸收良好的化合物;双向转运的P_(app)比值接近1,推测在Caco-2细胞单层模型的吸收过程中均无载体转运参与,为单纯的透细胞被动转运;欧前胡素、异欧前胡素和香柑内酯也有类似的转运趋势。禹白芷总香豆素给药,其中的水合氧化前胡内酯、比克白芷素和香柑内酯的P_(app)皆在1×10^(-5) cm·s^(-1)数量级,为吸收良好的化合物,这与单体化合物单独给药结果一致;欧前胡素、珊瑚菜内酯和异欧前胡素相对于单体给药,其P_(app)降低。虽然双向转运的P_(app)比值皆在0.5~1.5,但是相对于单体给药数值也存在波动,推测总香豆素中化合物之间可能存在相互作用。该文结果为研究含有禹白芷的复方中药的禹白芷香豆素的肠吸收研究奠定了基础。