Liver fibrosis in mice treated with Yue-Ju-Bao-He pills

Background:The aim of this study was to investigate the protective effect and antioxidant mechanism of Yue-Ju-Bao-He pills(YJBH)on liver fibrosis induced by carbon tetrachloride in mice.Methods:Thirty C57BL/6 mice were randomly divided into cntrol group,model group,and YJBH group.The control group received 0.2 mL olive oil intraperitoneally injected twice per week for six weeks.Four weeks after injection,the control group received oral treatment of 0.2 mL normal saline once per day for three weeks.The model and YJBH groups received 20%carbon tetrachloride olive oil solution(2 mL/kg)intraperitoneal injections twice per week for 6 weeks to induce liver fibrosis.After 4 weeks of carbon tetrachloride injections,the model and YJBH groups received oral treatment of either 0.2 mL normal saline or YJBH(0.9 mg/kg)once per day for 3 weeks,respectively.After three weeks of treatment,liver sections from mice were used for hematoxylin-eosin staining and Masson’s Trichrome staining to observe the status of the liver tissue.The therapeutic effect of YJBH on liver fibrosis was studied by determining alanine transaminase and aspartate aminotransferase and pathological changes in livers.Determining the malondialdehydelevel,superoxide dismutase,and glutathione peroxidase activities in liver homogenates were done to observe antioxidant mechanisms.Results:Compared with the control group,the body weight of mice in the model group decreased significantly and the liver index increased.After YJBH treatment,the body weight of mice increased and the liver index decreased compared with the model group.In addition,liver function indexes aspartate aminotransferase and alanine transaminase were significantly improved.The level of malondialdehyde in liver tissue was significantly decreased,and the expression of glutathione peroxidase and superoxide dismutase were increased.The pathological examination showed that liver cell injury of YJBH group was significantly reduced by the infiltration of inflammatory cells into collagen fibers.Conclusion:YJBH can effectively delay the progress of liver fibrosis induced by carbon tetrachloride,and the mechanism may be related to oxidative stress.

越鞠保和丸联合辛伐他汀对动脉粥样硬化小鼠心肌纤维化的作用

目的:观察越鞠保和丸联用辛伐他汀能否增强对动脉粥样硬化引起的心肌纤维化的逆转作用。方法:采用高脂饲料饲养ApoE-/-小鼠建立动脉粥样硬化斑块模型和心肌纤维化模型,越鞠保和丸和辛伐他汀联用和单用对ApoE-/-小鼠进行干预,同时设立高脂饲料饲养的模型对照组和普通饲料饲养的正常对照组,Elisa法检测小鼠血清血脂、血管紧张素Ⅱ(AngⅡ)、基质金属蛋白酶-1(MMP-1)含量,心肌组织masson染色定量分析胶原容积分数(CVF),高通量转录组测序分析各组心肌组织差异表达基因。结果:越鞠保和丸和辛伐他汀联用相比单用能更有效的降低总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL-C)、AngⅡ和升高高密度脂蛋白(HDL-C)、MMP-1,并且masson染色CVF值也明显降低,从高通量转录组测序差异基因分析结果来看,越鞠保和丸联合辛伐他汀与正常对照组相比,差异基因表达主要集中在脂肪酸代谢、延长及降解等相关通路上,而与模型对照组相比,差异基因表达主要集中在原发性免疫缺陷和肠道免疫网络的IgA的产生等相关通路上。结论:越鞠保和丸联合辛伐他汀可以更有效的逆转动脉粥样硬化导致的心肌纤维化,其机制与调控免疫炎性反应有关。