EVERY Sunday morning, a group of young women head for the China Drama Institute located in the south of the city, where they practice, rehearse and discuss skills of Yueju opera. They are Huang Min and her friends, al...EVERY Sunday morning, a group of young women head for the China Drama Institute located in the south of the city, where they practice, rehearse and discuss skills of Yueju opera. They are Huang Min and her friends, all authentic Beijingers. Apart from their normal work and study, they have a common hobby:singing Yueju opera. It is the Yueju art that binds them. Talking to Huang Min and her friends,one immediately feels the passion展开更多
目的:通过分析越鞠丸的有效成分,研究其治疗股骨头坏死和慢性疼痛伴抑郁症异病同治的作用机制。方法:借助中药系统药理学数据库与分析平台(TCMSP)检索越鞠丸的化学成分和作用靶点,采用Uniprot查询与靶点对应的基因,应用GeneCards数据库...目的:通过分析越鞠丸的有效成分,研究其治疗股骨头坏死和慢性疼痛伴抑郁症异病同治的作用机制。方法:借助中药系统药理学数据库与分析平台(TCMSP)检索越鞠丸的化学成分和作用靶点,采用Uniprot查询与靶点对应的基因,应用GeneCards数据库查询与股骨头坏死及慢性疼痛伴抑郁症两种疾病相关的基因。将越鞠丸4味中药关键成分的靶点信息分别和股骨头坏死以及慢性疼痛伴抑郁症的靶点基因进行相互映射,进而运用CytoScape 3.9.1分别构建两疾病的化合物-靶点(基因)网络、蛋白质-蛋白质相互作用网络,筛选出两者的核心靶点。通过DAVID进行基因本体论(GO)功能富集分析和京都基因与基因组百科全书(KEGG)通路富集分析。最后进行靶点-通路网络构建以及分子对接分析,研究越鞠丸对两者的作用机制。结果:越鞠丸的4种关键化合物(槲皮素、山柰酚、豆甾醇、β-谷甾醇)均同时作用于股骨头坏死和慢性疼痛伴抑郁症的靶点基因,两疾病共有的核心基因为白细胞介素(Interleukin,IL)-6、IL-1B、肿瘤抑制蛋白p53(Tumor Protein p53,TP53)、基质金属蛋白酶(Matrix Metallopeptidase 9,MMP9),GO富集结果显示生物过程-基因表达的正向调节、分子功能-酶结合、细胞组分-细胞外空间和细胞外区域为两种疾病共有部分,KEGG通路分析后取交集发现晚期糖基化终末产物(Advanced Glycation End-product,AGE)与其受体(Receptor for Advanced Glycation End Products,RAGE)信号通路、脂质和动脉粥样硬化通路、流体剪切应力和动脉粥样硬化通路以及癌症通路是两种疾病共通的信号通路;分子对接结果显示,槲皮素、山柰酚与MMP9结合最优,豆甾醇、β-谷甾醇与TP53-结合蛋白1结合最优。结论:越鞠丸以多种活性成分、多个作用靶点和途径发挥其异病同治股骨头坏死和慢性疼痛伴抑郁症的作用,为越鞠丸的临证运用提供理论依据。展开更多
文摘EVERY Sunday morning, a group of young women head for the China Drama Institute located in the south of the city, where they practice, rehearse and discuss skills of Yueju opera. They are Huang Min and her friends, all authentic Beijingers. Apart from their normal work and study, they have a common hobby:singing Yueju opera. It is the Yueju art that binds them. Talking to Huang Min and her friends,one immediately feels the passion
文摘目的:通过分析越鞠丸的有效成分,研究其治疗股骨头坏死和慢性疼痛伴抑郁症异病同治的作用机制。方法:借助中药系统药理学数据库与分析平台(TCMSP)检索越鞠丸的化学成分和作用靶点,采用Uniprot查询与靶点对应的基因,应用GeneCards数据库查询与股骨头坏死及慢性疼痛伴抑郁症两种疾病相关的基因。将越鞠丸4味中药关键成分的靶点信息分别和股骨头坏死以及慢性疼痛伴抑郁症的靶点基因进行相互映射,进而运用CytoScape 3.9.1分别构建两疾病的化合物-靶点(基因)网络、蛋白质-蛋白质相互作用网络,筛选出两者的核心靶点。通过DAVID进行基因本体论(GO)功能富集分析和京都基因与基因组百科全书(KEGG)通路富集分析。最后进行靶点-通路网络构建以及分子对接分析,研究越鞠丸对两者的作用机制。结果:越鞠丸的4种关键化合物(槲皮素、山柰酚、豆甾醇、β-谷甾醇)均同时作用于股骨头坏死和慢性疼痛伴抑郁症的靶点基因,两疾病共有的核心基因为白细胞介素(Interleukin,IL)-6、IL-1B、肿瘤抑制蛋白p53(Tumor Protein p53,TP53)、基质金属蛋白酶(Matrix Metallopeptidase 9,MMP9),GO富集结果显示生物过程-基因表达的正向调节、分子功能-酶结合、细胞组分-细胞外空间和细胞外区域为两种疾病共有部分,KEGG通路分析后取交集发现晚期糖基化终末产物(Advanced Glycation End-product,AGE)与其受体(Receptor for Advanced Glycation End Products,RAGE)信号通路、脂质和动脉粥样硬化通路、流体剪切应力和动脉粥样硬化通路以及癌症通路是两种疾病共通的信号通路;分子对接结果显示,槲皮素、山柰酚与MMP9结合最优,豆甾醇、β-谷甾醇与TP53-结合蛋白1结合最优。结论:越鞠丸以多种活性成分、多个作用靶点和途径发挥其异病同治股骨头坏死和慢性疼痛伴抑郁症的作用,为越鞠丸的临证运用提供理论依据。
