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Point Charge between Two Grounded and Conducting Planes Forming a 60°Angle and for Yukawa’s Potential and Coulomb’s Potential
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作者 Miguel Angel López-Mariño Omar Olmos López José Manuel Pardo Regueiro 《Journal of Electromagnetic Analysis and Applications》 2021年第4期57-66,共10页
<p> For a point charge between two grounded conductor planes forming a 60<span style="white-space:nowrap;">°</span> angle, the potential and electric field generated by point charge fo... <p> For a point charge between two grounded conductor planes forming a 60<span style="white-space:nowrap;">°</span> angle, the potential and electric field generated by point charge for Yukawa’s potential (<span style="white-space:nowrap;">e<sup><span style="white-space:nowrap;">-</span><em>μr</em></sup>/<em>r</em></span>) and Coulomb’s potential (1/<em>r</em>) are modeled and simulated. The expression for the potential that generalizes the cases discussed in López-Mari<span style="white-space:nowrap;"><span style="white-space:nowrap;">&#241;</span></span>o, M. and Trujillo Caballero, J. (2017) Point Charges and Conducting Planes for Yukawa’s Potential and Coulomb’s Potential. <em>Journal of Electromagnetic Analysis and Applications</em>, 9, 135-146. </p> <p> <a href="https://doi.org/10.4236/jemaa.2017.910012" target="_blank">https://doi.org/10.4236/jemaa.2017.910012</a> is presented. Graphs for the potential and electric field for both cases are showed using Maple, that of Coulomb and that of Yukawa for different values of <span style="white-space:nowrap;"><em>μ</em></span> . The purpose of this work is to offer students a practical guide for problem analysis of electrostatics using Maple’s capabilities as a computational tool. </p> 展开更多
关键词 yukawa’s potential Coulomb’s potential Method of Images
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Point Charges and Conducting Planes for Yukawa’s Potential and Coulomb’s Potential 被引量:1
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作者 M.A.Lopez-Marino Juan C.Trujillo Caballero 《Journal of Electromagnetic Analysis and Applications》 2017年第10期135-146,共12页
In this work, we modeled and simulated the electric potential generated by point charges in the region of grounded conductor planes for Yukawa potential (e&minus;μ/r) and Coulomb potential (1/r). We show the symb... In this work, we modeled and simulated the electric potential generated by point charges in the region of grounded conductor planes for Yukawa potential (e&minus;μ/r) and Coulomb potential (1/r). We show the symbolic expression for the electric potential and some graphs for it and for the electric field with different values of μ. We observe that the electric potential decreases as the value of μ increases and that does not allow all the charge to be distributed on the surface of the conductor. 展开更多
关键词 yukawa potential Coulomb potential Method of Images
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Dirac equation for the Hulthén potential within the Yukawa-type tensor interaction
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作者 Oktay Aydogdu Elham Maghsoodi Hassan Hassanabadi 《Chinese Physics B》 SCIE EI CAS CSCD 2013年第1期68-76,共9页
Using the Nikiforov-Uvarov (NU) method, pseudospin and spin symmetric solutions of the Dirac equation for the scalar and vector Hulthen potentials with the Yukawa-type tensor potential are obtained for an arbitrary ... Using the Nikiforov-Uvarov (NU) method, pseudospin and spin symmetric solutions of the Dirac equation for the scalar and vector Hulthen potentials with the Yukawa-type tensor potential are obtained for an arbitrary spin-orbit coupling quantum number K. We deduce the energy eigenvalue equations and corresponding upper- and lower-spinor wave functions in both the pseudospin and spin symmetry cases. Numerical results of the energy eigenvalue equations and the upper- and lower-spinor wave functions are presented to show the effects of the external potential and particle mass parameters as well as pseudospin and spin symmetric constants on the bound-state energies and wave functions in the absence and presence of the tensor interaction. 展开更多
关键词 Dirac equation Hulthen potential and yukawa potential pseudospin and spin symmetry tensor interaction
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The Yukawa potential in semirelativistic formulation via supersymmetry quantum mechanics
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作者 S.Hassanabadi M.Ghominejad +1 位作者 S.Zarrinkamar H.Hassanabadi 《Chinese Physics B》 SCIE EI CAS CSCD 2013年第6期171-174,共4页
We apply an approximation to the centrifugal term and solve the two-body spinless-Salpeter equation (SSE) with the Yukawa potential via the supersymmetric quantum mechanics (SUSYQM) for arbitrary quantum numbers. ... We apply an approximation to the centrifugal term and solve the two-body spinless-Salpeter equation (SSE) with the Yukawa potential via the supersymmetric quantum mechanics (SUSYQM) for arbitrary quantum numbers. Useful figures and tables are also included. 展开更多
关键词 spinless-salpeter equation two-body-system supersymmetry quantum mechanics yukawa potential
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Approximate solution of the spin-one Duffin-Kemmer-Petiau(DKP) equation under a non-minimal vector Yukawa potential in(1+1)-dimensions
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作者 H.Hassanabadi Z.Molaee 《Chinese Physics B》 SCIE EI CAS CSCD 2012年第12期74-77,共4页
We solve the Duffin-Kemmer-Petiau (DKP) equation with a non-minimal vector Yukawa potential in (1+1)- dimensional spa^e-time for spin-1 particles. The Nikiforov Uvarov method is used in the calculations, and the ... We solve the Duffin-Kemmer-Petiau (DKP) equation with a non-minimal vector Yukawa potential in (1+1)- dimensional spa^e-time for spin-1 particles. The Nikiforov Uvarov method is used in the calculations, and the eigen- functions as well as the energy eigenvalues are obtained in a proper Pekeris-type approximation. 展开更多
关键词 DKP equation non-minimal vector yukawa potential Nikiforov-Uvarov method
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Spinless particles in the field of unequal scalar vector Yukawa potentials
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作者 M.Hamzavi S.M.Ikhdair K.E.Thylwe 《Chinese Physics B》 SCIE EI CAS CSCD 2013年第4期81-86,共6页
We present analytical bound state solutions of the spin-zero Klein–Gordon (KG) particles in the field of unequal mix-ture of scalar and vector Yukawa potentials within the framework of the approximation scheme to t... We present analytical bound state solutions of the spin-zero Klein–Gordon (KG) particles in the field of unequal mix-ture of scalar and vector Yukawa potentials within the framework of the approximation scheme to the centrifugal potential term for any arbitrary l-state. The approximate energy eigenvalues and unnormalized wave functions are obtained in closed forms using a simple shortcut of the Nikiforov–Uvarov (NU) method. Further, we solve the KG–Yukawa problem for its exact numerical energy eigenvalues via the amplitude phase (AP) method to test the accuracy of the present solutions found by using the NU method. Our numerical tests using energy calculations demonstrate the existence of inter-dimensional degeneracy amongst the energy states of the KG–Yukawa problem. The dependence of the energy on the dimension D is numerically discussed for spatial dimensions D = 2–6. 展开更多
关键词 Klein–Gordon equation yukawa potential D-dimensional space Nikiforov–Uvarov and amplitude phase methods
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Approximate Analytical Solution of the Yukawa Potential with Arbitrary Angular Momenta
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作者 M.Hamzavi M.Movahedi +1 位作者 K.-E.Thylwe A.A.Rajabi 《Chinese Physics Letters》 SCIE CAS CSCD 2012年第8期16-19,共4页
The Yukawa potential is often used to compute bound-state normalizations and energy levels of neutral atoms.By using the generalized parametric Nikiforov–Uvarov method,we obtain approximate analytical solutions of th... The Yukawa potential is often used to compute bound-state normalizations and energy levels of neutral atoms.By using the generalized parametric Nikiforov–Uvarov method,we obtain approximate analytical solutions of the radial Schrödinger equation for the Yukawa potential.The energy eigenvalues and the corresponding eigenfunctions are calculated in closed forms.Some numerical results are presented and show that these results are in good agreement with those obtained previously by other methods.Also,we find the energy levels of the familiar pure Coulomb potential energy levels when the screening parameter of the Yukawa potential goes to zero. 展开更多
关键词 potential METHODs yukawa
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Non-Relativistic Bound State Solutions of Modified Quadratic Yukawa plus <i>q</i>-Deformed Eckart Potential
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作者 Akaninyene D. Antia Ituen B. Okon +1 位作者 Akaninyene O. Akankpo Jonah B. Usanga 《Journal of Applied Mathematics and Physics》 2020年第4期660-671,共12页
We have obtained approximate bound state solutions of Schr&#246;dinger wave equation with modified quadratic Yukawa plus q-deformed Eckart potential Using Parametric Nikiforov-Uvarov (NU) method. However, we obtai... We have obtained approximate bound state solutions of Schr&#246;dinger wave equation with modified quadratic Yukawa plus q-deformed Eckart potential Using Parametric Nikiforov-Uvarov (NU) method. However, we obtained numerical energy eigenvalues and un-normalized wave function using confluent hypergeometric function (Jacobi polynomial). With some modifications, our potential reduces to a well-known potential such as Poschl-Teller and exponential inversely quadratic potential. Numerical bound state energies were carried out using a well-designed Matlab algorithm while the plots were obtained using origin software. The result obtained is in agreement with that of the existing literature. 展开更多
关键词 schrodinger Equation Nikiforov-Uvarov Method Modified QUADRATIC yukawa PLUs q-Deformed Eckart potential
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Comments on“Non-Relativistic Treatment of a Generalized Inverse Quadratic Yukawa Potential”[Chin. Phys.Lett.34(2017)110301]
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作者 R.C.Woods 《Chinese Physics Letters》 SCIE CAS CSCD 2020年第8期200-200,共1页
Some problems with the article by Oluwadare and Oyewumi[Chin.Phys.Lett.34(2017)110301]are discussed.The previously proposed solution of the Schrödinger wave equation in the generalized inverse quadratic Yukawa po... Some problems with the article by Oluwadare and Oyewumi[Chin.Phys.Lett.34(2017)110301]are discussed.The previously proposed solution of the Schrödinger wave equation in the generalized inverse quadratic Yukawa potential is unsatisfactory for a number of reasons. 展开更多
关键词 yukawa s. inverse
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Non-Relativistic Treatment of a Generalized Inverse Quadratic Yukawa Potential
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作者 Oluwatimilehin Oluwadare Kayode Oyewumi 《Chinese Physics Letters》 SCIE CAS CSCD 2017年第11期1-5,共5页
A bound state solution is a quantum state solution of a particle subjected to a potential such that the particle's energy is less than the potential at both negative and positive infinity. The particle's energy may ... A bound state solution is a quantum state solution of a particle subjected to a potential such that the particle's energy is less than the potential at both negative and positive infinity. The particle's energy may also be negative as the potential approaches zero at infinity. It is characterized by the discretized eigenvalues and eigenfunctions, which contain all the necessary information regarding the quantum systems under consideration. The bound state problems need to be extended using a more precise method and approximation scheme. This study focuses on the non-relativistic bound state solutions to the generalized inverse quadratic Yukawa potential. The expression for the non-relativistic energy eigenvalues and radial eigenfunctions are derived using proper quantization rule and formula method, respectively. The results reveal that both the ground and first excited energy eigenvalues depend largely on the angular momentum numbers, screening parameters, reduced mass, and the potential depth. The energy eigenvalues, angular momentum numbers, screening parameters, reduced mass, and the potential depth or potential coupling strength determine the nature of bound state of quantum particles. The explored model is also suitable for explaining both the bound and continuum states of quantum systems. 展开更多
关键词 In Non-Relativistic Treatment of a Generalized Inverse Quadratic yukawa potential
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SHUBIN REGULARITY FOR THE RADIALLY SYMMETRIC SPATIALLY HOMOGENEOUS BOLTZMANN EQUATION WITH DEBYE-YUKAWA POTENTIAL
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作者 Leo GLANGETAS Haoguang LI 《Acta Mathematica Scientia》 SCIE CSCD 2019年第6期1487-1507,共21页
In this work, we study the Cauchy problem for the radially symmetric spatially homogeneous Boltzmann equation with Debye-Yukawa potential. We prove that this Cauchy problem enjoys the same smoothing effect as the Cauc... In this work, we study the Cauchy problem for the radially symmetric spatially homogeneous Boltzmann equation with Debye-Yukawa potential. We prove that this Cauchy problem enjoys the same smoothing effect as the Cauchy problem defined by the evolution equation associated to a fractional logarithmic harmonic oscillator. To be specific, we can prove the solution of the Cauchy problem belongs to Shubin spaces. 展开更多
关键词 BOLTZMANN equation shubin REGULARITY spectral decomposition Debye-yukawa potential
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China’s Potential Economic Growths during 2015-2025 under Different Scenarios~ 被引量:1
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作者 李平 娄峰 《China Economist》 2015年第2期82-99,共18页
China has entered a crucial period of further growth and restructuring that will last until 2025. Based on a review of the major factors affecting China's potential economic growth, this paper has created an integrat... China has entered a crucial period of further growth and restructuring that will last until 2025. Based on a review of the major factors affecting China's potential economic growth, this paper has created an integrated economic system model consisting of system dynamics, econometrics, and input-output for the forecast of China's economic size and structure by 2025. Analysis shows that prior to 2025, China will be able to maintain a potential annual economic growth rate of 5.7%-7.2%. Faced with an international environment of a possible slowdown of advanced economies and diminishing demographic dividends, China needs to further expedite its urbanization process, enhance R&D and education spending, increase total factor productivity (TFP), vigorously develop the tertiary sector, and expand consumption in order to achieve the optimistically estimated growth of 6.6%-7.4% during the period of 2015-2025. Economic growth should be accompanied by the upgrade of industry structure and improvement of investment and consumption structures. 展开更多
关键词 China's economy structural transition potential economic growth rate
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Size dependency and potential field influence on deriving mechanical properties of carbon nanotubes using molecular dynamics 被引量:3
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作者 K.G.S.Dilrukshi M.A.N.Dewapriya U.G.A.Puswewala 《Theoretical & Applied Mechanics Letters》 CAS CSCD 2015年第4期167-172,共6页
A thorough understanding on the mechanical properties of carbon nanotube (CNT) is essential in extending the advanced applications of CNT based systems. However, conducting experiments to estimate mechanical propert... A thorough understanding on the mechanical properties of carbon nanotube (CNT) is essential in extending the advanced applications of CNT based systems. However, conducting experiments to estimate mechanical properties at this scale is extremely challenging. Therefore, development of mechanistic models to estimate the mechanical properties of CNTs along with the integration of existing continuum mechanics concepts is critically important. This paper presents a comprehensive molecular dynamics simulation study on the size dependency and potential function influence of mechanical properties of CNT. Commonly used reactive bond order (REBO) and adaptive intermolecular reactive bond order (A1REBO) potential functions were considered in this regard. Young's modulus and shear modulus of CNTs are derived by integrating classical continuum mechanics concepts with molecular dynamics simulations. The results indicate that the potential function has a significant influence on the estimated mechanical properties of CNTs, and the influence of potential field is much higher when studying the torsional behaviour of CNTs than the tensile behaviour. 展开更多
关键词 Carbon nanotubes Molecular dynamics potential field shear modulus Poisson's ratio
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Momentum Eigensolutions of Feinberg-Horodecki Equation with Time-Dependent Screened Kratzer-Hellmann Potential 被引量:1
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作者 Mahmoud Farout Sameer M. Ikhdair 《Journal of Applied Mathematics and Physics》 2020年第7期1207-1221,共15页
We obtain an approximate value of the quantized momentum eigenvalues, <em>P<sub>n</sub></em>, together with the space-like coherent eigenvectors for the space-like counterpart of the Schr<sp... We obtain an approximate value of the quantized momentum eigenvalues, <em>P<sub>n</sub></em>, together with the space-like coherent eigenvectors for the space-like counterpart of the Schr<span style="white-space:nowrap;">&#246;</span>dinger equation, the Feinberg-Horodecki equation, with a screened Kratzer-Hellmann potential which is constructed by the temporal counterpart of the spatial form of this potential. In addition, we got exact eigenvalues of the momentum and the eigenstates by solving Feinberg-Horodecki equation with Kratzer potential. The present work is illustrated with three special cases of the screened Kratzer-Hellman potential: the time-dependent screened Kratzer potential, time-dependent Hellmann potential and, the time-dependent screened Coulomb potential. 展开更多
关键词 Coherent states Feinberg-Horodecki Equation Time-Dependent screened Kratzer-Hellmann potential Time-Dependent Kratzer potential Time-Dependent yukawa potential Time-Dependent Hellmann potential Time-Dependent Coulomb potential
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Daikenchuto (Da-Jian-Zhong-Tang) ameliorates intestinal fibrosis by activating myofibroblast transient receptor potential ankyrin 1 channel 被引量:3
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作者 Keizo Hiraishi Lin-Hai Kurahara +8 位作者 Miho Sumiyoshi Yao-Peng Hu Kaori Koga Miki Onitsuka Daibo Kojima Lixia Yue Hidetoshi Takedatsu Yu-Wen Jian Ryuji Inoue 《World Journal of Gastroenterology》 SCIE CAS 2018年第35期4036-4053,共18页
AIM To investigate the anti-fibrotic effects of the traditional oriental herbal medicine Daikenchuto(DKT) associated with transient receptor potential ankyrin 1(TRPA1) channels in intestinal myofibroblasts. METHODS In... AIM To investigate the anti-fibrotic effects of the traditional oriental herbal medicine Daikenchuto(DKT) associated with transient receptor potential ankyrin 1(TRPA1) channels in intestinal myofibroblasts. METHODS Inflammatory and fibrotic changes were detected in a2,4,6-trinitrobenzenesulfonic acid(TNBS) chronic colitis model of wild-type and TRPA1-knockout(TRPA1-KO) mice via pathological staining and immunoblotting analysis.Ca^(2+) imaging experiments examined the effects of DKT and its components/ingredients on intestinal myofibroblast(In Myo Fib) cell TRPA1 channel function.Profibrotic factors and transforming growth factor (TGF) -β1-associated signaling were tested in an In Myo Fib cell line by q PCR and immunoblotting experiments.Samples from non-stenotic and stenotic regions of the intestines of patients with Crohn’s disease (CD) were used for pathological analysis. RESULTS Chronic treatment with TNBS caused more severe inflammation and fibrotic changes in TRPA1-KO than in wild-type mice.A one-week enema administration of DKT reduced fibrotic lesions in wild-type but not in TRPA1-KO mice.The active ingredients of DKT,i.e.,hydroxyα-sanshool and 6-shogaol,induced Ca^(2+) influxes in In Myo Fib,and this was antagonized by co-treatment with a selective TRPA1 channel blocker,HC-030031.DKT counteracted TGF-β1-induced expression of TypeⅠcollagen andα-smooth muscle actin (α-SMA) ,which were accompanied by a reduction in the phosphorylation of Smad-2 and p38-mitogen-activated protein kinase (p38-MAPK) and the expression of myocardin.Importantly,24-h incubation with a DKT active component Japanese Pepper increased the m RNA and protein expression levels of TRPA1 in In Myo Fibs,which in turn negatively regulated collagen synthesis.In the stenotic regions of the intestines of CD patients,TRPA1 expression was significantly enhanced.CONCLUSION The effects of DKT on the expression and activation of the TRPA1 channel could be advantageous for suppressing intestinal fibrosis,and benefit inflammatory bowel disease treatment. 展开更多
关键词 Intestinal fibrosis MYOFIBROBLAsT Transient receptor potential ANKYRIN 1 Crohn’s disease Collagen α-smooth muscle ACTIN
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The therapeutic potential of mesenchymal stem cells in Alzheimer's disease:converging mechanisms 被引量:3
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作者 Gadi Turgeman 《Neural Regeneration Research》 SCIE CAS CSCD 2015年第5期698-699,共2页
Mesenchymal stem cells (MSCS) are pluripotent stem cells isolated from various tissues, but mostly from bone marrow, adipose tissue, and umbilical cord blood. Well known for their mesenchymal lineages differentiati... Mesenchymal stem cells (MSCS) are pluripotent stem cells isolated from various tissues, but mostly from bone marrow, adipose tissue, and umbilical cord blood. Well known for their mesenchymal lineages differentiation (e.g., bone, cartilage and fat tissues), it was suggested that MSCs possess plasticity prop- erties enabling them to differentiate into non-mesenchymal lineages. Indeed, several protocols claimed for differentiating MSCs to neurons in vitro, but concern was raised for the ef- fectiveness and in vivo relevance of such differentiation. Thus, though their neurogenic differentiation properties are still in debate, they were nevertheless, suggested as candidates for treat- ing neurodegenerative disorders such as Parkinson's diseases, multiple sclerosis and Alzheimer's disease (AD). 展开更多
关键词 CELL sTEM The therapeutic potential of mesenchymal stem cells in Alzheimer’s disease MsCs
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MULTIPLE SOLUTIONS FOR NONHOMOGENEOUS SCHRDINGER-POISSON EQUATIONS WITH SIGN-CHANGING POTENTIAL 被引量:1
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作者 王丽霞 马世旺 许娜 《Acta Mathematica Scientia》 SCIE CSCD 2017年第2期555-572,共18页
In this article, we study the following nonhomogeneous Schrodinger-Poissone quations{-△u+λV(x)u+K(x)Фu=f(x,u)+g(x),x∈R^3,-△Ф=k(x)u^2, x∈R^3}where λ 〉 0 is a parameter. Under some suitable assumpt... In this article, we study the following nonhomogeneous Schrodinger-Poissone quations{-△u+λV(x)u+K(x)Фu=f(x,u)+g(x),x∈R^3,-△Ф=k(x)u^2, x∈R^3}where λ 〉 0 is a parameter. Under some suitable assumptions on 11, K, f and g, the existence of multiple solutions is proved by using the Ekeland's variational principle and the Mountain Pass Theorem in critical point theory. In particular, the potential V is allowed to be signchanging. 展开更多
关键词 NONHOMOGENEOUs sign-changing potential schrOdinger-Poisson equations Eke-land's variational principle Mountain Pass Theorem
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From the Green's Function in Tight-Binding Representation to Interatomic Many-body Potentials 被引量:1
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作者 Shaojun LIU(Department of physics, Beijing Normal University, Beijing 100875, China)Xiaobo WEI(Department of Physics, Liuzhou College of Education, Liuzhou 545005, China)Zhaodou CHEN Jun CAI and Benkun MA(Department of Physics, Beijing Normal University, 《Journal of Materials Science & Technology》 SCIE EI CAS CSCD 1999年第4期315-319,共5页
In this paper, we deduce the analytical form of many-body interatomic potentials based on the Green's function in tight-binding representation. The many-body potentials are expressed as the functions of the hoppin... In this paper, we deduce the analytical form of many-body interatomic potentials based on the Green's function in tight-binding representation. The many-body potentials are expressed as the functions of the hopping integrals which are the physical origin of cohesion of atoms. For thesimple case of s-valent system, the inversion of the many-body potentials are discussed in detail by using the lattice inversion method. 展开更多
关键词 Rev Li CHEN From the Green’s Function in Tight-Binding Representation to Interatomic Many-body potentials body
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Bacterial melanin in rat models of Parkinson's disease: a potential neuroprotective strategy 被引量:1
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作者 Tigran Petrosyan 《Neural Regeneration Research》 SCIE CAS CSCD 2015年第2期211-212,共2页
Melanins are widely used in medicine, pharmacology and cosmetics. Different technologies have been used to obtain melanin including: chemical synthesis based on oxidation of tyrosine and its derivatives; extraction f... Melanins are widely used in medicine, pharmacology and cosmetics. Different technologies have been used to obtain melanin including: chemical synthesis based on oxidation of tyrosine and its derivatives; extraction from animal materials; alkaline extraction from plant material; and microbiological synthesis. A few number of works have been published that were focused on purification of water insoluble 3,4-dihy- droxy-phenylalanine-melanins (Kukulianskaia et al., 2002). The majority of synthetic and natural melanins are insoluble in wa- ter that significantly complicates preparation of pharmacolog- ical and cosmetic preparations. Obtaining of low-cost soluble biotechnological melanin can speed up application of melanin in medicine and other fields. For the first time, melanin-syn-thesizing strain with high level of pigment synthesis - Bacillus thuringiensis was obtained. The ecologically safe technology of biosynthesis, isolation and purification of the bacterial melanin has been elaborated. 展开更多
关键词 a potential neuroprotective strategy Bacterial melanin in rat models of Parkinson’s disease
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JTE-522-induced apoptosis in human gastric adenocarinoma cell line AGS cells by caspase activation accompanying cytochrome C release,membrane translocation of Bax and loss of mitochondrial membrane potential 被引量:16
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作者 Hong-Liang Li Xiao-Hong Li Jun-Hua Lü Xian-Da Ren,Department of Pharmacology,Jinan University Pharmacy College,Guangzhou 510632,Guangdong Province,China Dan-Dan Chen,Department of Cardiology,First Affiliated Hospital,Zhongshan University,Guangzhou 510089,Guangdong Province,China Hai-Wei Zhang,Department of Pathology,Jinan University Medical College,Guangzhou 510632,Guangdong Province,China Cun-Chuan Wang,Department of laparoscopic surgery,First Affiliated Hospital,Jinan University Medical College,Guangzhou 510632,Guangdong Province,China 《World Journal of Gastroenterology》 SCIE CAS CSCD 2002年第2期217-223,共7页
AIM: To investigate the role of the mitochondrial pathway in JTE-522-induced apoptosis and to investigate the relationship between cytochrome C release, caspase activity and loss of mitochondrial membrane potential (D... AIM: To investigate the role of the mitochondrial pathway in JTE-522-induced apoptosis and to investigate the relationship between cytochrome C release, caspase activity and loss of mitochondrial membrane potential (Deltapsim). METHODS: Cell culture, cell counting, ELISA assay, TUNEL, flow cytometry, Western blot and fluorometric assay were employed to investigate the effect of JTE-522 on cell proliferation and apoptosis in AGS cells and related molecular mechanism. RESULTS: JTE-522 inhibited the growth of AGS cells and induced the apoptosis. Caspases 8 and 9 were activated during apoptosis as judged by the appearance of cleavage products from procaspase and the caspase activities to cleave specific fluorogenic substrates. To elucidate whether the activation of caspases 8 and 9 was required for the apoptosis induction, we examined the effect of caspase-specific inhibitors on apoptosis. The results showed that caspase inhibitors significantly inhibited the apoptosis induced by JTE-522. In addition, the membrane translocation of Bax and cytosolic release of cytochrome C accompanying with the decrease of the uptake of Rhodamin 123, were detected at an early stage of apoptosis. Furthermore, Bax translocation, cytochrome C release, and caspase 9 activation were blocked by Z-VAD.fmk and Z-IETD-CHO. CONCLUSION: The present data indicate a crucial association between activation of caspases 8, 9, cytochrome C release, membrane translocation of Bax, loss of Deltapsim and JTE-522-induced apoptosis in AGS cells. 展开更多
关键词 Adenocarcinoma stomach Neoplasms Amino Acid Chloromethyl Ketones Anti-Inflammatory Agents Non-steroidal Apoptosis BENZENEsULFONATEs CAsPAsEs inhibitors Cyclooxygenase Inhibitors Cysteine Proteinase Inhibitors Cytochrome c Group Enzyme Activation Humans In situ Nick-End Labeling Membrane potentials Mitochondria OXAZOLEs Proto-Oncogene Proteins Proto-Oncogene Proteins c-bcl-2 Research support Non-U.s. Gov't Tumor Cells Cultured bcl-2-Associated X Protein
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