[Objective] The paper was to discuss regulation and protection effect of Yupingfeng polysaccharide （YPF-P） on immunosuppressive mice. [Method] Taking immunosuppressive mice induced by 80 mg/kg cyclophosphamide as te...[Objective] The paper was to discuss regulation and protection effect of Yupingfeng polysaccharide （YPF-P） on immunosuppressive mice. [Method] Taking immunosuppressive mice induced by 80 mg/kg cyclophosphamide as test animal model, effects of different doses （100,200 and 400 mg/kg） of YPF-P on spleen index, serum IL-2 and IFN-γ levels of mice were studied. [ Result] High dose of YPF-P （400 mg/kg） could improve the spleen index of immunosuppressive mice （P 〈0.05）. YPF-P could enhance serum IL-2 and IFN-γ levels of immunosuppressive mice, and the effects in middle-dose group and low-dose group were better. YPF-P could significantly increase serum IL-2 level of normal mice （ P 〈 0.01 ）, but it had no significant effect on serum IFN-γ level of normal mice. [ Conclusion] The research provides an experimental basis for clinical application of YPF-P.展开更多
目的:研究扶正祛邪双表法对流感病毒感染小鼠肺组织TLR4/NF-κB信号通路的调控作用。方法:将昆明种小鼠随机分为模型组、正常组、解表法代表方剂银翘散(YQS)组、固表法代表方剂玉屏风散(YPF)组、双表法代表方剂(YPS)组以及利巴韦林组。...目的:研究扶正祛邪双表法对流感病毒感染小鼠肺组织TLR4/NF-κB信号通路的调控作用。方法:将昆明种小鼠随机分为模型组、正常组、解表法代表方剂银翘散(YQS)组、固表法代表方剂玉屏风散(YPF)组、双表法代表方剂(YPS)组以及利巴韦林组。采用流感病毒滴鼻方法建立病毒感染模型,根据给药量制备相应浓度药物并灌胃给药,正常组和模型组给予同体积生理盐水。采用RT-PCR、免疫组化法检测给药后1、3、5、7天各时间点各组小鼠Toll样受体4(toll like receptors 4,TLR4)和核转录因子κB(nuclear factor-κB,NF-κB)蛋白及其mRNA动态表达情况。结果:YQS、YPF及YPS在不同时点均能不同程度阻断流感小鼠肺组织中TLR4、NF-κB蛋白及其mRNA的高表达,并且YPS组表达最低,差异有统计学意义(P<0.05)。结论:双表法对流感病毒感染小鼠的防治作用与抑制流感病毒感染小鼠肺组织TLR4/NF-κB信号通路过度活化有关。展开更多
基金Supported by Natural Science Fund of Guangdong Province(S2013010012191)Science and Technology Project of Guangdong Province(2009B020307004)
文摘[Objective] The paper was to discuss regulation and protection effect of Yupingfeng polysaccharide （YPF-P） on immunosuppressive mice. [Method] Taking immunosuppressive mice induced by 80 mg/kg cyclophosphamide as test animal model, effects of different doses （100,200 and 400 mg/kg） of YPF-P on spleen index, serum IL-2 and IFN-γ levels of mice were studied. [ Result] High dose of YPF-P （400 mg/kg） could improve the spleen index of immunosuppressive mice （P 〈0.05）. YPF-P could enhance serum IL-2 and IFN-γ levels of immunosuppressive mice, and the effects in middle-dose group and low-dose group were better. YPF-P could significantly increase serum IL-2 level of normal mice （ P 〈 0.01 ）, but it had no significant effect on serum IFN-γ level of normal mice. [ Conclusion] The research provides an experimental basis for clinical application of YPF-P.
文摘目的:探讨玉屏风散治疗非小细胞肺癌(Non-Small Cell Lung Cancer,NSCLC)的潜在靶点及作用机制。方法:检索中药系统药理学数据库与分析平台(TCMSP)V2.0获得玉屏风散的活性成分;运用TCMSP筛选活性成分靶点,利用Uniprot数据库对靶点进行标准化命名;通过GeneCards、TTD等数据库查询NSCLC相关靶点;利用VennDiagram得到“中药化合物-疾病”共同靶点。应用String数据库和Cytoscape软件构建蛋白质-蛋白质相互作用网络图。筛选核心作用靶点,进行基因本体论(GO)功能富集分析和京都基因与基因组百科全书(KEGG)通路富集分析。结果:获得玉屏风散防治NSCLC的活性成分44个,其中槲皮素、汉黄芩素等活性成分作用明显。筛选得到潜在作用靶点85个,包括信号传导及转录激活因子1(Signal Transducer and Activator of Transcription 1,STAT1)、半胱氨酸天冬氨酸酶(Caspase,CASP)3等。在GO分析中,玉屏风散防治NSCLC共涉及生物学过程1551个、细胞组分47个和分子功能146个,生物学过程主要涉及类固醇激素反应、细胞对化学压力的反应等;细胞组分主要涉及膜筏、膜微区等;分子功能主要涉及核受体活性、配体激活转录因子活性等。KEGG通路富集分析的结果显示,玉屏风散主要涉及细胞凋亡、磷脂酰肌醇-3-激酶(PI3K)/蛋白激酶B(Akt)信号通路等通路。结论:玉屏风散治疗NSCLC涉及多个活性成分、作用靶点和关键信号通路。
文摘目的:研究扶正祛邪双表法对流感病毒感染小鼠肺组织TLR4/NF-κB信号通路的调控作用。方法:将昆明种小鼠随机分为模型组、正常组、解表法代表方剂银翘散(YQS)组、固表法代表方剂玉屏风散(YPF)组、双表法代表方剂(YPS)组以及利巴韦林组。采用流感病毒滴鼻方法建立病毒感染模型,根据给药量制备相应浓度药物并灌胃给药,正常组和模型组给予同体积生理盐水。采用RT-PCR、免疫组化法检测给药后1、3、5、7天各时间点各组小鼠Toll样受体4(toll like receptors 4,TLR4)和核转录因子κB(nuclear factor-κB,NF-κB)蛋白及其mRNA动态表达情况。结果:YQS、YPF及YPS在不同时点均能不同程度阻断流感小鼠肺组织中TLR4、NF-κB蛋白及其mRNA的高表达,并且YPS组表达最低,差异有统计学意义(P<0.05)。结论:双表法对流感病毒感染小鼠的防治作用与抑制流感病毒感染小鼠肺组织TLR4/NF-κB信号通路过度活化有关。
