[Objectives]This study was conducted to clarify the pathogens causing suspected sugarcane leaf scald symptoms in sugarcane plants found in the Xinping sugarcane area of Yunnan in 2019.[Methods]The XAF1/XAR1 specific p...[Objectives]This study was conducted to clarify the pathogens causing suspected sugarcane leaf scald symptoms in sugarcane plants found in the Xinping sugarcane area of Yunnan in 2019.[Methods]The XAF1/XAR1 specific primers of Xanthomonas albilineans were used to detect 8 suspected samples from Xinping County,Yunnan Province.[Results]The specific band 600 bp in size was detected.The sequencing results showed that the sequences detected were all 608 bp,and were 100%identical.The BLAST search results showed that the obtained sequence was 100%identical to the nucleotide sequence of X.albilineans GPE PC73 strain(GenBank accession number:FP565176)from France,and they were located in the same branch of the phylogenetic tree.According to the field symptom diagnosis and molecular identification results,the sugarcane disease in Xinping,Yunnan was confirmed to be the quarantine disease sugarcane leaf scald caused by X.allbilineasn.[Conclusions]The pathogens were identified by molecular biological identification methods,providing a scientific basis for the prevention and control of leaf scald disease in the future.展开更多
[Objective] This study aimed to select suitable varieties of maize for Ailao mountainous areas, [Method] A comparison test of eight varieties of maize was conducted in Shengli Village, Mosha Town, Xinping County, Yunn...[Objective] This study aimed to select suitable varieties of maize for Ailao mountainous areas, [Method] A comparison test of eight varieties of maize was conducted in Shengli Village, Mosha Town, Xinping County, Yunnan Province from May to October in 2016. [Result] The yield of Shenyu 10 was up to 66.3.4 kg/ 667 m^2 and there were extremely significant differences between Shenyu 10 and Xuanhuangdan 5 （CK）. Moreover, it had strong stress resistance. As Jingdan 12, Beiju 0913, Ante 8000, Yunrui 89 and Yayu 78 were planted in the region, their earing should not appear in rainy months. The inciderce of stem rot and ear rot of Yayu 891 was high, so Yayu 891 should not be planted in the region. [Conclusion] Shenyu 10 had a high yield, suffered few diseases and had strong stress resistance, so it can be popularized widely in the region.展开更多
基金Special Fund for Construction of Sugar Crop Research System(CARS-170303)Yunling Industrial Technology Leading Talent Training Project:Sugarcane Pest Control(2018LJRC56)Special Fund for the Construction of Modern Agricultural Industry Technology System in Yunnan Province.
文摘[Objectives]This study was conducted to clarify the pathogens causing suspected sugarcane leaf scald symptoms in sugarcane plants found in the Xinping sugarcane area of Yunnan in 2019.[Methods]The XAF1/XAR1 specific primers of Xanthomonas albilineans were used to detect 8 suspected samples from Xinping County,Yunnan Province.[Results]The specific band 600 bp in size was detected.The sequencing results showed that the sequences detected were all 608 bp,and were 100%identical.The BLAST search results showed that the obtained sequence was 100%identical to the nucleotide sequence of X.albilineans GPE PC73 strain(GenBank accession number:FP565176)from France,and they were located in the same branch of the phylogenetic tree.According to the field symptom diagnosis and molecular identification results,the sugarcane disease in Xinping,Yunnan was confirmed to be the quarantine disease sugarcane leaf scald caused by X.allbilineasn.[Conclusions]The pathogens were identified by molecular biological identification methods,providing a scientific basis for the prevention and control of leaf scald disease in the future.
文摘[Objective] This study aimed to select suitable varieties of maize for Ailao mountainous areas, [Method] A comparison test of eight varieties of maize was conducted in Shengli Village, Mosha Town, Xinping County, Yunnan Province from May to October in 2016. [Result] The yield of Shenyu 10 was up to 66.3.4 kg/ 667 m^2 and there were extremely significant differences between Shenyu 10 and Xuanhuangdan 5 （CK）. Moreover, it had strong stress resistance. As Jingdan 12, Beiju 0913, Ante 8000, Yunrui 89 and Yayu 78 were planted in the region, their earing should not appear in rainy months. The inciderce of stem rot and ear rot of Yayu 891 was high, so Yayu 891 should not be planted in the region. [Conclusion] Shenyu 10 had a high yield, suffered few diseases and had strong stress resistance, so it can be popularized widely in the region.
