OBJECTIVE:To observe the clinical efficacy on improving the quality of meibum in patients suffer from dry eye disease(DED)due to meibomian gland dysfunction(MGD)with hyperactivity of Yang due to Yin deficiency pattern...OBJECTIVE:To observe the clinical efficacy on improving the quality of meibum in patients suffer from dry eye disease(DED)due to meibomian gland dysfunction(MGD)with hyperactivity of Yang due to Yin deficiency pattern after being treated with Pinggan Yuyin Qingre formula(平肝育阴清热方,PGYYQR).METHODS:Totally 120 patients who met the inclusion criteria were included and stratified into three levels according to the level of MGD(1-3),and patients in each level was randomly allocated into the treatment group and control group according to a 1∶1 ratio.Both groups were treated with sodium hyaluronate eye drops,and the treatment group was also given PGYYQR granules.Both groups were treated continuously for eight weeks.The score of the properties of meibomian gland(MG)secretion,the score of the palpebral margins,the average noninvasive tear breakup time(NITBUTav),lipid layer thickness(LLT),and Traditional Chinese Medicine(TCM)syndrome efficacy were compared between the two groups after treatment.RESULTS:A total of 116 cases were included in the statistical analysis.The differences were statistically significant in the score of the properties of MG secretion,the score of the palpebral margins,and NITBUTav between the two groups after treatment,the treatment group was superior to the control group;there was no evidence of a difference in LLT.In terms of TCM syndrome efficacy,the total effective rate was 84.7%in the treatment group and 50.9%in the control group,with the statistically significant difference.None of the included cases had adverse reactions.CONCLUSIONS:PGYYQR is effective in improving the quality of meibum,and the tear film stability which thereby relieving the ocular symptoms in MGD-related DED patients with hyperactivity of Yang due to Yin deficiency pattern.展开更多
Objective: To study the effect of Yuyin recipe (愈银方, YYR), a Ch inese composite recipe, on cultured human epithelial cell proliferation in vitro .Methods: YYR in different concentrations was added separately in ...Objective: To study the effect of Yuyin recipe (愈银方, YYR), a Ch inese composite recipe, on cultured human epithelial cell proliferation in vitro .Methods: YYR in different concentrations was added separately in to the keratinocyte CoLo-16 line, after the latter had been cultured 24 hrs, to be co-cultured for different periods of time (24-72 hrs). A blank contro l group was set up in the meantime. Effect of YYR with different concentrations and different acting time on keratinocyte proliferation was observed by MTT meth od.Results: YYR in different concentrations showed significant inhib itory effect on keratinocyte CoLo-16 line in concentration-dependent and a cting-time-dependent manner.Conclusion: Inhibition on over proliferation of epithelial cells may be one of the mechanisms of YYR in curing psoriasis.展开更多
Objective:The objective of this study was to observe the effect of Bushen Qingre Yuyin Decoction on the spleen index,submandibular gland index,salivary secretion,submandibular gland morphology,and aquaporin 5(AQP5)exp...Objective:The objective of this study was to observe the effect of Bushen Qingre Yuyin Decoction on the spleen index,submandibular gland index,salivary secretion,submandibular gland morphology,and aquaporin 5(AQP5)expression levels in the submandibular gland.Materials and Methods:Fifty 8-week-old nonobese diabetic(NOD)female mice were randomly divided into the following five groups:model;hydroxychloroquine;and high-,medium-,and low-dose Bushen Qingre Yuyin Decoction prescription.The blank group consisted of ten 8-week-old Balb/C rats.Bushen Yuyin Qingre Decoction was administered by gavage at 20,40,and 80 g/kg in the low-,medium-,and high-dose groups,respectively.The hydroxychloroquine group was administered hydroxychloroquine at a dose of 80 mg/kg by gavage.The normal and model groups were administered the same amount of saline.After the different administrations,the amount of salivary secretion in the mice was regularly observed.After 12 weeks,the mice were sacrificed,and their submandibular gland tissues were excised,stained with hematoxylin-eosin,and pathologically scored.AQP5 expression levels in the tissues were detected using real-time polymerase chain reaction.Results:The spleen index of mice in the high-dose group was higher than that in the model group(P<0.05),and the submandibular gland index of the hydroxychloroquine group and all the Bushen Qingre Yuyin Decoction prescription groups was higher than that in the model group(P<0.05).After 6 weeks,the salivary volume of the hydroxychloroquine group and all the Bushen Qingre Yuyin Decoction prescription groups was significantly higher than that of the model group(P<0.05);the amount of lymphocyte infiltration in mice among each administration group was significantly reduced(P<0.05);and the AQP5 expression levels in the submandibular glands of mice had significantly increased in the hydroxychloroquine and high-dose groups(P<0.05),with the high-dose group showing the best effect.Conclusion:Bushen Qingre Yuyin Decoction can effectively increase the spleen and submandibular gland indexes of NOD mice,increase the amount of salivary secretion,reduce lymphocyte infiltration in submandibular gland tissue,and significantly increase AQP5 expression levels,which may be positively correlated with the administered dose.展开更多
基金Capital Health Development Research Project:Clinical Efficacy of the Pinggan Yuyin Qingre Formula in Treating Evaporative Dry Eye Disease Causing by Meibomian Gland Dysfunction(No.2016-2-2055)Zhi Nan National Famous and Senior Chinese Medicine Experts Inheritance Studio Construction Project[No.75(2022),the Personnel and Education Department of the National Administration of Traditional Chinese Medicine promulgated]the Sixth Batch of Beijing Municipal Academic Experience Inheritance Project for Traditional Chinese Medicine Experts[No.160(2021),Beijing Administration of Traditional Chinese Medicine promulgated]。
文摘OBJECTIVE:To observe the clinical efficacy on improving the quality of meibum in patients suffer from dry eye disease(DED)due to meibomian gland dysfunction(MGD)with hyperactivity of Yang due to Yin deficiency pattern after being treated with Pinggan Yuyin Qingre formula(平肝育阴清热方,PGYYQR).METHODS:Totally 120 patients who met the inclusion criteria were included and stratified into three levels according to the level of MGD(1-3),and patients in each level was randomly allocated into the treatment group and control group according to a 1∶1 ratio.Both groups were treated with sodium hyaluronate eye drops,and the treatment group was also given PGYYQR granules.Both groups were treated continuously for eight weeks.The score of the properties of meibomian gland(MG)secretion,the score of the palpebral margins,the average noninvasive tear breakup time(NITBUTav),lipid layer thickness(LLT),and Traditional Chinese Medicine(TCM)syndrome efficacy were compared between the two groups after treatment.RESULTS:A total of 116 cases were included in the statistical analysis.The differences were statistically significant in the score of the properties of MG secretion,the score of the palpebral margins,and NITBUTav between the two groups after treatment,the treatment group was superior to the control group;there was no evidence of a difference in LLT.In terms of TCM syndrome efficacy,the total effective rate was 84.7%in the treatment group and 50.9%in the control group,with the statistically significant difference.None of the included cases had adverse reactions.CONCLUSIONS:PGYYQR is effective in improving the quality of meibum,and the tear film stability which thereby relieving the ocular symptoms in MGD-related DED patients with hyperactivity of Yang due to Yin deficiency pattern.
文摘Objective: To study the effect of Yuyin recipe (愈银方, YYR), a Ch inese composite recipe, on cultured human epithelial cell proliferation in vitro .Methods: YYR in different concentrations was added separately in to the keratinocyte CoLo-16 line, after the latter had been cultured 24 hrs, to be co-cultured for different periods of time (24-72 hrs). A blank contro l group was set up in the meantime. Effect of YYR with different concentrations and different acting time on keratinocyte proliferation was observed by MTT meth od.Results: YYR in different concentrations showed significant inhib itory effect on keratinocyte CoLo-16 line in concentration-dependent and a cting-time-dependent manner.Conclusion: Inhibition on over proliferation of epithelial cells may be one of the mechanisms of YYR in curing psoriasis.
基金The Famous Doctor Inherits Workstation of“3+3 Inheritance Program”of Beijing Administration of Traditional Chinese Medicine
文摘Objective:The objective of this study was to observe the effect of Bushen Qingre Yuyin Decoction on the spleen index,submandibular gland index,salivary secretion,submandibular gland morphology,and aquaporin 5(AQP5)expression levels in the submandibular gland.Materials and Methods:Fifty 8-week-old nonobese diabetic(NOD)female mice were randomly divided into the following five groups:model;hydroxychloroquine;and high-,medium-,and low-dose Bushen Qingre Yuyin Decoction prescription.The blank group consisted of ten 8-week-old Balb/C rats.Bushen Yuyin Qingre Decoction was administered by gavage at 20,40,and 80 g/kg in the low-,medium-,and high-dose groups,respectively.The hydroxychloroquine group was administered hydroxychloroquine at a dose of 80 mg/kg by gavage.The normal and model groups were administered the same amount of saline.After the different administrations,the amount of salivary secretion in the mice was regularly observed.After 12 weeks,the mice were sacrificed,and their submandibular gland tissues were excised,stained with hematoxylin-eosin,and pathologically scored.AQP5 expression levels in the tissues were detected using real-time polymerase chain reaction.Results:The spleen index of mice in the high-dose group was higher than that in the model group(P<0.05),and the submandibular gland index of the hydroxychloroquine group and all the Bushen Qingre Yuyin Decoction prescription groups was higher than that in the model group(P<0.05).After 6 weeks,the salivary volume of the hydroxychloroquine group and all the Bushen Qingre Yuyin Decoction prescription groups was significantly higher than that of the model group(P<0.05);the amount of lymphocyte infiltration in mice among each administration group was significantly reduced(P<0.05);and the AQP5 expression levels in the submandibular glands of mice had significantly increased in the hydroxychloroquine and high-dose groups(P<0.05),with the high-dose group showing the best effect.Conclusion:Bushen Qingre Yuyin Decoction can effectively increase the spleen and submandibular gland indexes of NOD mice,increase the amount of salivary secretion,reduce lymphocyte infiltration in submandibular gland tissue,and significantly increase AQP5 expression levels,which may be positively correlated with the administered dose.
文摘目的 观察潜阳育阴颗粒对阴虚阳亢型高血压早期肾损害患者的临床疗效。方法 将60例阴虚阳亢型老年高血压早期肾损害病例,随机分为对照组(30例)和观察组(30例),对照组常规使用西药氨氯地平(或者联合缬沙坦)降压,观察组在对照组降压治疗维持达标的基础上服用中药潜阳育阴颗粒。24周后比较2组患者治疗前后的临床疗效、单光子发射计算机化体层显像术(SPECT)检测肾小球滤过率(GFR)和胱抑素C(Cyst-C)、尿β2微球蛋白(β2-MG)以及尿N 乙酰β-D葡萄糖苷酶(NAG)等早期肾损害指标的变化情况。结果 治疗后观察组和对照组的有效率分别为96%和50%,2组比较有显著性差异, P < 0.01;2组肾损害指标Cyst-C、β2-MG水平均有显著改善( P < 0.05 ),观察组较对照组改善效果更为显著( P < 0.05 );观察组肾损害指标(GFR、NAG)有显著改善( P < 0.05 );观察组优于对照组( P < 0.05 )。结论 潜阳育阴颗粒在降压达标基础上能够有效减少高血压患者的早期肾脏损害,改善临床症状。
