AIM： To investigate the role of vasoactive intestinal peptide（VIP） in form-deprivation myopia（FDM）.METHODS： FDM was created in three groups of eight chicks by placing a translucent diffuser on their right eyes.I...AIM： To investigate the role of vasoactive intestinal peptide（VIP） in form-deprivation myopia（FDM）.METHODS： FDM was created in three groups of eight chicks by placing a translucent diffuser on their right eyes.Intravitreal injections of saline and VIP were applied once a day into the occluded eyes of groups 2 and 3,respectively.Retinoscopy and axial length（AL） measurements were performed on the first and 8^th days of diffuser wear.The retina mR NA levels of the VIP receptors and the ZENK protein in right eyes of the three groups and left eyes of the first group on day 8 were determined using real time polymerase chain reaction（PCR）.RESULTS： The median final refraction（D） in right eyes were-13.75（-16.00,-12.00）,-11.50（-12.50,-7.50）,and-1.50（-4.75,-0.75） in groups 1,2,and 3,respectively（P〈0.001）.The median AL（mm） in right eyes were 10.65（10.00,11.10）,9.90（9.70,10.00）,and 9.20（9.15,9.25） in groups 1,2,and 3,respectively（P〈0.001）.The median delta-delta cycle threshold（CT） values for the VIP2 receptors were 1.07（0.82,1.43）,1.22（0.98,1.65）,0.29（0.22,0.45） in right eyes of groups 1,2,and 3,and 1.18（0.90,1.37） in left eyes of group 1,respectively（P=0.001）.The median delta-delta CT values for the ZENK protein were 1.07（0.63,5.03）,3.55（2.20,5.55）,undetectable in right eyes of groups 1,2,and 3 and 1.89（0.21,4.73） in left eyes of group 1,respectively（P=0.001）.CONCLUSION： VIP has potential inhibitory effects in the development of FDM.展开更多
The study of defense behavior development in pied flycatcher altricial nestlings has shown that preceding visual deprivation decreased the capability to freeze in the young. At the same time, auditory thresholds of de...The study of defense behavior development in pied flycatcher altricial nestlings has shown that preceding visual deprivation decreased the capability to freeze in the young. At the same time, auditory thresholds of deprived nestlings were lower than those of the nestlings developing in unaltered visual environment. Our goal in the present work was to study effects of visual input on the functional development of neural circuitry for flycatcher’s acoustically-guided defensive behavior. We compared the immunohistochemical response of transcription factors ZENK and c-Fos in the nestlings’ auditory telencephalic structures in visually deprived and non-deprived flycatcher nestlings to alarm calls emitted by adult birds. We have demonstrated that the development of flycatcher nestlings under the conditions of limited visual afferentation results in the decrease of induction of the immediate-early genes c-Fos and ZENK in auditory structures of 9-day-old nestlings performing auditory-guided defense behavior. The most marked decrease of induction of both genes is observed in Field L. Our data suggest that visual afferentation affects neuronal activations in higher auditory structures of nestlings’ brain that, in turn, may increase the efficiency of species-typical alarm call. Thus, the work demonstrated for the first time that visual affererntation facilitates the growth of efficiency of alarm call affecting the activity of neurons in auditory integrative structures.展开更多
基金Supported by the Eskisehir Osmangazi University Scientific Research Project(No.2011/11034)Commission
文摘AIM： To investigate the role of vasoactive intestinal peptide（VIP） in form-deprivation myopia（FDM）.METHODS： FDM was created in three groups of eight chicks by placing a translucent diffuser on their right eyes.Intravitreal injections of saline and VIP were applied once a day into the occluded eyes of groups 2 and 3,respectively.Retinoscopy and axial length（AL） measurements were performed on the first and 8^th days of diffuser wear.The retina mR NA levels of the VIP receptors and the ZENK protein in right eyes of the three groups and left eyes of the first group on day 8 were determined using real time polymerase chain reaction（PCR）.RESULTS： The median final refraction（D） in right eyes were-13.75（-16.00,-12.00）,-11.50（-12.50,-7.50）,and-1.50（-4.75,-0.75） in groups 1,2,and 3,respectively（P〈0.001）.The median AL（mm） in right eyes were 10.65（10.00,11.10）,9.90（9.70,10.00）,and 9.20（9.15,9.25） in groups 1,2,and 3,respectively（P〈0.001）.The median delta-delta cycle threshold（CT） values for the VIP2 receptors were 1.07（0.82,1.43）,1.22（0.98,1.65）,0.29（0.22,0.45） in right eyes of groups 1,2,and 3,and 1.18（0.90,1.37） in left eyes of group 1,respectively（P=0.001）.The median delta-delta CT values for the ZENK protein were 1.07（0.63,5.03）,3.55（2.20,5.55）,undetectable in right eyes of groups 1,2,and 3 and 1.89（0.21,4.73） in left eyes of group 1,respectively（P=0.001）.CONCLUSION： VIP has potential inhibitory effects in the development of FDM.
文摘The study of defense behavior development in pied flycatcher altricial nestlings has shown that preceding visual deprivation decreased the capability to freeze in the young. At the same time, auditory thresholds of deprived nestlings were lower than those of the nestlings developing in unaltered visual environment. Our goal in the present work was to study effects of visual input on the functional development of neural circuitry for flycatcher’s acoustically-guided defensive behavior. We compared the immunohistochemical response of transcription factors ZENK and c-Fos in the nestlings’ auditory telencephalic structures in visually deprived and non-deprived flycatcher nestlings to alarm calls emitted by adult birds. We have demonstrated that the development of flycatcher nestlings under the conditions of limited visual afferentation results in the decrease of induction of the immediate-early genes c-Fos and ZENK in auditory structures of 9-day-old nestlings performing auditory-guided defense behavior. The most marked decrease of induction of both genes is observed in Field L. Our data suggest that visual afferentation affects neuronal activations in higher auditory structures of nestlings’ brain that, in turn, may increase the efficiency of species-typical alarm call. Thus, the work demonstrated for the first time that visual affererntation facilitates the growth of efficiency of alarm call affecting the activity of neurons in auditory integrative structures.
