为探究干酪乳酪杆菌Zhang(Lacticaseibacillus casei Zhang)在酸和低温胁迫下能否形成活的非可培养(viable but non-culturable,VBNC)状态。该实验将L.casei Zhang置于不同pH值(2.5、3、4、5、6)的MRS液体培养基中,在4℃条件下进行酸和...为探究干酪乳酪杆菌Zhang(Lacticaseibacillus casei Zhang)在酸和低温胁迫下能否形成活的非可培养(viable but non-culturable,VBNC)状态。该实验将L.casei Zhang置于不同pH值(2.5、3、4、5、6)的MRS液体培养基中,在4℃条件下进行酸和低温胁迫,不同时间点取样,然后采用平板计数法、荧光显微镜和流式细胞术3种方法对胁迫后的菌体进行活性测定。实验结果显示,低温条件下L.casei Zhang在pH值为2.5、3、5、6胁迫下未获得VBNC态;L.casei Zhang在pH 4的MRS液体培养基低温胁迫120 d获得了VBNC态,革兰氏染色观察到VBNC态细胞长度变短变弯曲。该研究为L.casei Zhang VBNC态的机理研究提供了一定的参考依据。展开更多
OVER 180 years ago, a craftsman named Zhang Mingshan came to prominence in north China＇s Tian- jin for his colorful clay figurines. Zhang＇s work became so celebrated that he gained favor from the royal fam- ily of t...OVER 180 years ago, a craftsman named Zhang Mingshan came to prominence in north China＇s Tian- jin for his colorful clay figurines. Zhang＇s work became so celebrated that he gained favor from the royal fam- ily of the Qing Dynasty （1644-1911）. who decided to decorate the royal palace with his work. At the time, the best craftsmen in the country were sponsored by the stateand separated from the business world, so throughout his life most of Zhang＇s pieces went to the palace or to friends; few were sold for profit.展开更多
本试验旨在通过优化培养条件提高植物乳杆菌Zhang-LL发酵培养液中的菌体浓度,为获得高活菌数的益生菌产品奠定基础。对植物乳杆菌的发酵培养基中的氮源进行单因素筛选,选取501油菜籽蛋白胨作为MRS培养基的替代氮源。基于优化的培养基成...本试验旨在通过优化培养条件提高植物乳杆菌Zhang-LL发酵培养液中的菌体浓度,为获得高活菌数的益生菌产品奠定基础。对植物乳杆菌的发酵培养基中的氮源进行单因素筛选,选取501油菜籽蛋白胨作为MRS培养基的替代氮源。基于优化的培养基成分,采用单因素及4因素3水平L9(34)正交试验优化发酵条件,确定最优发酵条件为;501油菜籽蛋白胨含量为3.0%、温度34℃、p H 6.5、接种量7%,发酵罐流加15%Na2CO3,转速为150 r/min;在此优化条件下,发酵液活菌数可达1.07×1010CFU/m L。展开更多
The Zhang Paper Workshop preserving the complete traditional papermaking process has been praised by experts as “the living fossil of Chinese papermaking”. Due to the impact of industrial papermaking, however, the t...The Zhang Paper Workshop preserving the complete traditional papermaking process has been praised by experts as “the living fossil of Chinese papermaking”. Due to the impact of industrial papermaking, however, the traditional papermaking has gradually lost its market advantage, and the Zhang Paper Workshop prevailing for a time is already on the decline. In order to retain this precious cultural heritage, for more than 10 years, the Gleaners has spared no effort to provide various efforts to papermakers and has found a way out for the manual papermaking, and results have been achieved. How to protect and inherit the traditional papermaking process under the premise of conserving the ecological environment and maintaining the integrity of the papermaking process, and establish the Zhang Paper Workshop as a place for spreading the concept of nature conservation and cultural heritage protection is an urgent problem to be solved. This requires the support of policies, the investment of funds and the upgrading of technology, as well as the promotion of understanding and the cultivation of feelings.展开更多
文摘OVER 180 years ago, a craftsman named Zhang Mingshan came to prominence in north China＇s Tian- jin for his colorful clay figurines. Zhang＇s work became so celebrated that he gained favor from the royal fam- ily of the Qing Dynasty （1644-1911）. who decided to decorate the royal palace with his work. At the time, the best craftsmen in the country were sponsored by the stateand separated from the business world, so throughout his life most of Zhang＇s pieces went to the palace or to friends; few were sold for profit.
文摘本试验旨在通过优化培养条件提高植物乳杆菌Zhang-LL发酵培养液中的菌体浓度,为获得高活菌数的益生菌产品奠定基础。对植物乳杆菌的发酵培养基中的氮源进行单因素筛选,选取501油菜籽蛋白胨作为MRS培养基的替代氮源。基于优化的培养基成分,采用单因素及4因素3水平L9(34)正交试验优化发酵条件,确定最优发酵条件为;501油菜籽蛋白胨含量为3.0%、温度34℃、p H 6.5、接种量7%,发酵罐流加15%Na2CO3,转速为150 r/min;在此优化条件下,发酵液活菌数可达1.07×1010CFU/m L。
基金Sponsored by the National Social Science Fund of China(11CGL112)
文摘The Zhang Paper Workshop preserving the complete traditional papermaking process has been praised by experts as “the living fossil of Chinese papermaking”. Due to the impact of industrial papermaking, however, the traditional papermaking has gradually lost its market advantage, and the Zhang Paper Workshop prevailing for a time is already on the decline. In order to retain this precious cultural heritage, for more than 10 years, the Gleaners has spared no effort to provide various efforts to papermakers and has found a way out for the manual papermaking, and results have been achieved. How to protect and inherit the traditional papermaking process under the premise of conserving the ecological environment and maintaining the integrity of the papermaking process, and establish the Zhang Paper Workshop as a place for spreading the concept of nature conservation and cultural heritage protection is an urgent problem to be solved. This requires the support of policies, the investment of funds and the upgrading of technology, as well as the promotion of understanding and the cultivation of feelings.
