Objective:To investigate the effect of Wei’s triple nine needling therapy on the N2-P2 wave of the flash visual evoked potential(FVEP)in rats of the of the transverse directional pulling model.Methods:Thirty-six Wist...Objective:To investigate the effect of Wei’s triple nine needling therapy on the N2-P2 wave of the flash visual evoked potential(FVEP)in rats of the of the transverse directional pulling model.Methods:Thirty-six Wistar rats were randomly grouped,nine were in normal control group without any treatment,and the remaining 27 were surgically modelled in the right eyes.Eighteen of these rats were randomly divided into a Wei’s triple nine needling therapy group and a model group of nine rats each after the TON model was made using the transverse quantitative retraction method.The other 9 rats were sham-operated,and only the optic nerve was exposed without retraction.On the next day of modelling,the Wei’s triple nine needling therapy group was treated with Wei’s triple nine needling therapy for 20 minutes/1 time/1 day for 14 days.The model group,sham-operated group and normal control group were not intervened.Wei's triple nine acupoints were represented as follows:1st link:"Jingming"(BL1)and"Chengqi"(ST1);2nd link:"Sizhukong"(SJ23)penetrating"Taiyang"(EX-HN5);and the third:"Fengchi"(GB 20)and"Taichong"(LV3).The FVEP of each group was observed on 1d,7d and 14d.The FVEP of each group was observed on 1d,7d and 14d.Results:Compared with the model group,the N2 wave latency and P2 wave latency were shortened in the Wei’s triple nine needling therapy on 1d(P<0.05,P<0.01);on 7d,their N2 wave latency was shortened(P<0.01)and the N2-P2 wave amplitude was increased compared with the model group(P<0.05);on 14d,their P2 wave latency was shortened(P<0.05)and the N2-P2 wave amplitude was increased.There was no statistical difference in N2 wave latency,P2 wave latency and N2-P2 wave amplitude in the sham-operated group on 1d,7d and 14d(P>0.05).The delayed N2 and P2 wave latencies in the model group did not improve from 1d to 14d(P>0.05)and the amplitude decreased throughout,showing a significant difference on 14d compared to 1d(P<0.05).In contrast,the N2 wave latency in Wei’s triple nine needling therapy group was not significantly different until 7d to 14d(P<0.05);its P2 wave latency,although significantly delayed from 1d to 7d(P<0.05),recovered on 14d and reached a level that was not statistically different from 1d and 7d(P>0.05).There was also no significant decrease in N2-P2 wave amplitude between 1d and 14d in Wei’s triple nine needling therapy group(P>0.05).Conclusion:In this experiment,the TON rat model was successfully established by the transverse quantitative retraction method,and the treatment of TON rats with Wei’s triple nine needling therapy reduced the P2 wave delay of the FVEP electrophysiological signal and increased the N2-P2 amplitude,which had a certain positive effect on the repair of optic nerve injury,probably related to its effect of improving the conduction function of the optic nerve and protecting the retinal ganglion cells that had not been degenerated and necrosed.展开更多
＇Warming-dredging needling technique＇ is one of the specific needling techniques created by Prof. Zheng Kui-shan for treating intractable and difficult diseases. Developed from ＇Shao Shah Huo （Mountain-burning Fir...＇Warming-dredging needling technique＇ is one of the specific needling techniques created by Prof. Zheng Kui-shan for treating intractable and difficult diseases. Developed from ＇Shao Shah Huo （Mountain-burning Fire）＇ and ＇warming- reinforcing method＇, this method displays the effects of ＇warming＂ ＇dredging＇ and ＇reinforcing＇. The ＇scapula-penetrating heat＇ and ＇eye-transmitting heat＇ belong to the representative needling techniques of ＇warming-dredging needling technique＇. In order to promote the clinical application of ＇warming-dredging needling technique＂ its theoretical foundation and clinical experience are summarized.展开更多
He＇s clinical suited acupuncture manipulation is summarized by the famous tradi onal Chinese medical master Prof. HE Pu-ren in his clinical prac ces for almost 70 years,and it is highly favored as ＂flying needle man...He＇s clinical suited acupuncture manipulation is summarized by the famous tradi onal Chinese medical master Prof. HE Pu-ren in his clinical prac ces for almost 70 years,and it is highly favored as ＂flying needle manipula on＂. This manipula on has high requirements on finger strength; it is characterized from techniques for holding needles to needling inser on,and it is painless during needle insertion and the needling responses are rapid; the operations are simple,with high prac cability in clinic,and it can improve working effi ciency and clinical effi cacy.展开更多
基金National Natural Science Foundation of China(No.81904258)。
文摘Objective:To investigate the effect of Wei’s triple nine needling therapy on the N2-P2 wave of the flash visual evoked potential(FVEP)in rats of the of the transverse directional pulling model.Methods:Thirty-six Wistar rats were randomly grouped,nine were in normal control group without any treatment,and the remaining 27 were surgically modelled in the right eyes.Eighteen of these rats were randomly divided into a Wei’s triple nine needling therapy group and a model group of nine rats each after the TON model was made using the transverse quantitative retraction method.The other 9 rats were sham-operated,and only the optic nerve was exposed without retraction.On the next day of modelling,the Wei’s triple nine needling therapy group was treated with Wei’s triple nine needling therapy for 20 minutes/1 time/1 day for 14 days.The model group,sham-operated group and normal control group were not intervened.Wei's triple nine acupoints were represented as follows:1st link:"Jingming"(BL1)and"Chengqi"(ST1);2nd link:"Sizhukong"(SJ23)penetrating"Taiyang"(EX-HN5);and the third:"Fengchi"(GB 20)and"Taichong"(LV3).The FVEP of each group was observed on 1d,7d and 14d.The FVEP of each group was observed on 1d,7d and 14d.Results:Compared with the model group,the N2 wave latency and P2 wave latency were shortened in the Wei’s triple nine needling therapy on 1d(P<0.05,P<0.01);on 7d,their N2 wave latency was shortened(P<0.01)and the N2-P2 wave amplitude was increased compared with the model group(P<0.05);on 14d,their P2 wave latency was shortened(P<0.05)and the N2-P2 wave amplitude was increased.There was no statistical difference in N2 wave latency,P2 wave latency and N2-P2 wave amplitude in the sham-operated group on 1d,7d and 14d(P>0.05).The delayed N2 and P2 wave latencies in the model group did not improve from 1d to 14d(P>0.05)and the amplitude decreased throughout,showing a significant difference on 14d compared to 1d(P<0.05).In contrast,the N2 wave latency in Wei’s triple nine needling therapy group was not significantly different until 7d to 14d(P<0.05);its P2 wave latency,although significantly delayed from 1d to 7d(P<0.05),recovered on 14d and reached a level that was not statistically different from 1d and 7d(P>0.05).There was also no significant decrease in N2-P2 wave amplitude between 1d and 14d in Wei’s triple nine needling therapy group(P>0.05).Conclusion:In this experiment,the TON rat model was successfully established by the transverse quantitative retraction method,and the treatment of TON rats with Wei’s triple nine needling therapy reduced the P2 wave delay of the FVEP electrophysiological signal and increased the N2-P2 amplitude,which had a certain positive effect on the repair of optic nerve injury,probably related to its effect of improving the conduction function of the optic nerve and protecting the retinal ganglion cells that had not been degenerated and necrosed.
基金supported by Open Fund Projects of Zheng’s Acupuncture Manipulation Academic Schools Heritage Work Room Gansu ProvinceState Administration of Traditional Chinese Medicine(No.LP0128060-kfjj01,No.LP0128060-kfjj02)~~
文摘＇Warming-dredging needling technique＇ is one of the specific needling techniques created by Prof. Zheng Kui-shan for treating intractable and difficult diseases. Developed from ＇Shao Shah Huo （Mountain-burning Fire）＇ and ＇warming- reinforcing method＇, this method displays the effects of ＇warming＂ ＇dredging＇ and ＇reinforcing＇. The ＇scapula-penetrating heat＇ and ＇eye-transmitting heat＇ belong to the representative needling techniques of ＇warming-dredging needling technique＇. In order to promote the clinical application of ＇warming-dredging needling technique＂ its theoretical foundation and clinical experience are summarized.
文摘He＇s clinical suited acupuncture manipulation is summarized by the famous tradi onal Chinese medical master Prof. HE Pu-ren in his clinical prac ces for almost 70 years,and it is highly favored as ＂flying needle manipula on＂. This manipula on has high requirements on finger strength; it is characterized from techniques for holding needles to needling inser on,and it is painless during needle insertion and the needling responses are rapid; the operations are simple,with high prac cability in clinic,and it can improve working effi ciency and clinical effi cacy.
