Decaleside II is the novel trisaccharide isolated from the edible roots of Decalepis hamiltonii that belong to a new class of natural insecticides. In the present study we have investigated grain protection potential ...Decaleside II is the novel trisaccharide isolated from the edible roots of Decalepis hamiltonii that belong to a new class of natural insecticides. In the present study we have investigated grain protection potential and persistence activity of Decaleside II against stored-product pests such as Rhyzopertha dominica, Sitophilus oryzae, Tribolium castaneum and Callosobruchus chinensis. Decaleside II usually increased parental mortality and reduced F1 progeny in residual toxicity bioassays. At 7 days of the storage period of treated grains and pulses, the mortality was 100% of all four stored product insects. Both wheat and green gram treated with Decaleside II were totally free from infestation for the longer period of storage up to 210 d. Decaleside II being a natural trisaccharide, does not pose any toxic hazard from the treated grain and the lack of toxicity is attributed to the 1, 4 α linkage of the sugars which are easily hydrolyzed by the digestive enzymes such as glucosidases. Therefore, the insect selectivity and mammalian safety of Decaleside II make them highly suitable for use as novel grain protectants of natural origin.展开更多
Freeze drying has a deleterious effect on the viability of microorganisms. In front of this difficulty, the present study adopts response surface methodology to optimize the chemical compositions of protective agents ...Freeze drying has a deleterious effect on the viability of microorganisms. In front of this difficulty, the present study adopts response surface methodology to optimize the chemical compositions of protective agents to seek for maximum viability of Bifidobacterium longum BIOMA 5920 during freeze-drying. Through the compara- tive analysis of single protectant, the complex protective agents show better effect on the Bifidobacterium viability. Human-like collagen (HLC), trehalose and glycerol are confirmed as significant factors by Box-Behnken Design. The optimized formula for these three variables is tested as follows: HLC 1.23%, trehalose 11.50% and glycerol 4.65%. Under this formula, the viability is 88.23%, 39.67% higher in comparison to the control. The viable count is 1.07×10 9 cfu·g-1 , greatly exceeding the minimum viable count requirement (10 6 cfu·g-1 ).展开更多
A coacervation method with double emulsion strategy （w/o/w） was used to prepare immobilized Lactobacillus E1. Diatomite was chosen as the carrier for bacteria. Sodium alginate, dextrin and gelatin were used as prote...A coacervation method with double emulsion strategy （w/o/w） was used to prepare immobilized Lactobacillus E1. Diatomite was chosen as the carrier for bacteria. Sodium alginate, dextrin and gelatin were used as protective solutes for the preservation of Lactobacillus E1 and their effects on the storage viability during storage were discussed. The influence of storage temperature on the storage viability was also examined. The results show that high bacteria viable count over 109 cfu/g for an extended shelf life of 37 d can be achieved with 2% sodium alginate, 5% dextrin and 4% gelatin as protective solutes, at 10 ℃ of the storage temperature. This immobilized Lactobacillus E1 has potential use as functional food ingredient for both human dairy food and animal feedstuff.展开更多
Four varieties of winter wheat with different return green rates were used to analyze the plasma membrane stability and the factors that affect winter wheat in the frigid region during winter. The removal of reactive ...Four varieties of winter wheat with different return green rates were used to analyze the plasma membrane stability and the factors that affect winter wheat in the frigid region during winter. The removal of reactive oxygen species, the degree of plasma membrane impairment, water composition and content, and the changes in cell viability in the crowns, which contain the growing point, were studied during the period from cold acclimation to the deep freezing. The results showed that electrical conductivity which reflects the degree of plasma membrane damaged under low temperature was significantly correlated with the free water and the total water content. The malondialdehyde （MDA） content, which reflects the degree of membrane peroxidation, was very significantly correlated with superoxide dismutase （SOD）, peroxidase, and ascorbic acid. During the deep freezing period, the SOD activity and glutathione （GSH） content of the winter wheat varieties were relative to their cold resistance. During this period, the MDA stability, SOD, GSH, and the total water and the free water content might be used to identify the cold resistance of winter wheat varieties.展开更多
THE giant panda is a kind of endangered rare animal,the number of which is less than onethousand at present,specially distributed in China.According to the protective scheme issuedby The International Association of N...THE giant panda is a kind of endangered rare animal,the number of which is less than onethousand at present,specially distributed in China.According to the protective scheme issuedby The International Association of Nature Protection,stock population in captivity must展开更多
文摘Decaleside II is the novel trisaccharide isolated from the edible roots of Decalepis hamiltonii that belong to a new class of natural insecticides. In the present study we have investigated grain protection potential and persistence activity of Decaleside II against stored-product pests such as Rhyzopertha dominica, Sitophilus oryzae, Tribolium castaneum and Callosobruchus chinensis. Decaleside II usually increased parental mortality and reduced F1 progeny in residual toxicity bioassays. At 7 days of the storage period of treated grains and pulses, the mortality was 100% of all four stored product insects. Both wheat and green gram treated with Decaleside II were totally free from infestation for the longer period of storage up to 210 d. Decaleside II being a natural trisaccharide, does not pose any toxic hazard from the treated grain and the lack of toxicity is attributed to the 1, 4 α linkage of the sugars which are easily hydrolyzed by the digestive enzymes such as glucosidases. Therefore, the insect selectivity and mammalian safety of Decaleside II make them highly suitable for use as novel grain protectants of natural origin.
基金Supported by the National High Technology Research and Development Program of China (2007AA03Z456)the National Natural Science Foundation of China (20776119, 21076169 and 31000019)+5 种基金the Xi’an Research and Development Program(NC08005, YF07078)the Scientific Research Program of Shaanxi Provincial Department of Education,China(08JK452,08JK453,JG08181,2010JC21,2010JS107,2010JS108, 2010JK876 and 2010JS109)Shaanxi Provincial Scientific Technology Research and Development Program (2007K06-03, 2010JQ2012, SJ08B03)the Specialized Research Fund for the Doctoral Program of Higher Education of China (20096101120023, 20096101110014)NWU Graduate Innovation and Creativity Funds (08YSY17)Shaanxi Key Subject Program, China
文摘Freeze drying has a deleterious effect on the viability of microorganisms. In front of this difficulty, the present study adopts response surface methodology to optimize the chemical compositions of protective agents to seek for maximum viability of Bifidobacterium longum BIOMA 5920 during freeze-drying. Through the compara- tive analysis of single protectant, the complex protective agents show better effect on the Bifidobacterium viability. Human-like collagen (HLC), trehalose and glycerol are confirmed as significant factors by Box-Behnken Design. The optimized formula for these three variables is tested as follows: HLC 1.23%, trehalose 11.50% and glycerol 4.65%. Under this formula, the viability is 88.23%, 39.67% higher in comparison to the control. The viable count is 1.07×10 9 cfu·g-1 , greatly exceeding the minimum viable count requirement (10 6 cfu·g-1 ).
基金Funded by the National Natural Science Foundation of China (No.30670566)the ISTCP (No. 2006DFA33150)
文摘A coacervation method with double emulsion strategy （w/o/w） was used to prepare immobilized Lactobacillus E1. Diatomite was chosen as the carrier for bacteria. Sodium alginate, dextrin and gelatin were used as protective solutes for the preservation of Lactobacillus E1 and their effects on the storage viability during storage were discussed. The influence of storage temperature on the storage viability was also examined. The results show that high bacteria viable count over 109 cfu/g for an extended shelf life of 37 d can be achieved with 2% sodium alginate, 5% dextrin and 4% gelatin as protective solutes, at 10 ℃ of the storage temperature. This immobilized Lactobacillus E1 has potential use as functional food ingredient for both human dairy food and animal feedstuff.
基金Supported by the Postdoctoral Science Foundation of Heilongjiang Province(2010RCB17)Doctoral Research Startup Foundation of Northeast Agricultural University(LBH-Z10266)+2 种基金New Rural Project of Heilongjiang Provincial Department of EducationProject of Education Department in Heilongjiang Province(12521035)Postgraduate Innovation Project of Education Department in Heilongjiang Province (YJSCX2012-036HLJ)
文摘Four varieties of winter wheat with different return green rates were used to analyze the plasma membrane stability and the factors that affect winter wheat in the frigid region during winter. The removal of reactive oxygen species, the degree of plasma membrane impairment, water composition and content, and the changes in cell viability in the crowns, which contain the growing point, were studied during the period from cold acclimation to the deep freezing. The results showed that electrical conductivity which reflects the degree of plasma membrane damaged under low temperature was significantly correlated with the free water and the total water content. The malondialdehyde （MDA） content, which reflects the degree of membrane peroxidation, was very significantly correlated with superoxide dismutase （SOD）, peroxidase, and ascorbic acid. During the deep freezing period, the SOD activity and glutathione （GSH） content of the winter wheat varieties were relative to their cold resistance. During this period, the MDA stability, SOD, GSH, and the total water and the free water content might be used to identify the cold resistance of winter wheat varieties.
文摘THE giant panda is a kind of endangered rare animal,the number of which is less than onethousand at present,specially distributed in China.According to the protective scheme issuedby The International Association of Nature Protection,stock population in captivity must
