WTFZ] The interaction of adenosine triphosphate with divalent metal ions is important in biochemical functions. The effects of pH and metal ions Mg 2+ , Ca 2+ , Zn 2+ , Mn 2+ , and Co 2+ on the c...WTFZ] The interaction of adenosine triphosphate with divalent metal ions is important in biochemical functions. The effects of pH and metal ions Mg 2+ , Ca 2+ , Zn 2+ , Mn 2+ , and Co 2+ on the chemical shift of the phosphate group of ATP have been studied using Nuclear Magnetic Resonance. The chemical shift of the β-phosphate of ATP is the most sensitive to pH. Ca 2+ and Mg 2+ bind with the α- and β-phosphate groups of ATP. Zn 2+ binds to the adenosine ring hydrogen as well as to phosphate. The paramagnetic ions Mn 2+ and Co 2+ do not cause chemical shifts of the phosphate or proton peak. Mn 2+ and Co 2+ broaden the resonance peak only.[展开更多
文摘WTFZ] The interaction of adenosine triphosphate with divalent metal ions is important in biochemical functions. The effects of pH and metal ions Mg 2+ , Ca 2+ , Zn 2+ , Mn 2+ , and Co 2+ on the chemical shift of the phosphate group of ATP have been studied using Nuclear Magnetic Resonance. The chemical shift of the β-phosphate of ATP is the most sensitive to pH. Ca 2+ and Mg 2+ bind with the α- and β-phosphate groups of ATP. Zn 2+ binds to the adenosine ring hydrogen as well as to phosphate. The paramagnetic ions Mn 2+ and Co 2+ do not cause chemical shifts of the phosphate or proton peak. Mn 2+ and Co 2+ broaden the resonance peak only.[
