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ZNF217 expression correlates with the biological behavior of human ovarian cancer cells 被引量:1
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作者 Lilin Hang Min Zhang +2 位作者 Fanliang Meng Mei Zhong Jing Li 《The Chinese-German Journal of Clinical Oncology》 CAS 2014年第11期539-544,共6页
The aim of the study was to investigate the correlation of zinc-finger protein 217 (ZNF217) gone ex- pression with the biological behavior of human ovarian cancer HO-8910 cells. Methods: The expression of ZNF217 in... The aim of the study was to investigate the correlation of zinc-finger protein 217 (ZNF217) gone ex- pression with the biological behavior of human ovarian cancer HO-8910 cells. Methods: The expression of ZNF217 in ovarian carcinoma cell line:s was detected by RT-PCR and Western blot, respectively. The biological behaviors of the transfectants were investigated by MTT, in vitro Boyden chamber and in vivo invasion assay, respectively. Results: RT-PCR and Western blotting revealed that transfection of ZNF217 into the HO-8910 cells significantly increased their proliferation along with mark- edly enhanced in vitro and in vivo invasion and metastatic abilities. MTT assay showed that the proliferation ability of pEGFP- N1-ZNF217/HO-8910 cells was significantly higher than that of pEGFP-N1/HO-8910 cells and HO-8910 cells (P 〈 0.001). The Boyden chamber assay showed that the numbers of migrating pEGFP-N1-ZNF217/HO-8910, pEGFP-N1/HO-8910 and HO-8910 cells were (141.25 ± 13.91) cells/200 x field, (82.50 ± 11.73) cells/200 × field and (81.75 ± 12.12) cells/200 x field, respectively, with a significant difference between them (F = 29.274, P 〈 0.001). The nude mouse experiment showed that the in vivo tumor formation ability of pEGFP-N1-ZNF217/HO-8910 cells was significantly higher than that of pEGFP-N1/HO-8910 cells (P 〈 0.001). Conclusion: Based on these clinical and laboratory observations, we conclude that ZNF217 may contribute to ovarian cancer invasion and metastasis, and associated with worse clinical outcomes. We evaluated ZNF217's role as a biomarker of ovarian carcinogenesis and tumor progression in patient samples and explored possible molecular mechanisms in promoting tumor growth and invasion. 展开更多
关键词 ovaran cancer zinc-finger protein 217 znf217 gene gene expression PROLIFERATION INVASION tumor metastasis
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Cloning of a novel human zinc finger protein(ZNF199)cDNA
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作者 Peirong Hu Long Yu +3 位作者 Min Zhang Xiaoke Wang Qiang Tu Shouyuan Zhao 《Chinese Science Bulletin》 SCIE EI CAS 1999年第3期287-290,共4页
ZINC finger proteins play an important role in the regulation of gene expression by binding to DNA/RNA in a sequence-specific manner. Now nearly 200 human zinc finger genes have been cloned. According to the differenc... ZINC finger proteins play an important role in the regulation of gene expression by binding to DNA/RNA in a sequence-specific manner. Now nearly 200 human zinc finger genes have been cloned. According to the difference of the conservative domain, the zinc finger proteins can be classified into several types.Most of zinc finger proteins belong to the C<sub>2</sub>H<sub>2</sub> type containing the consensus amino acid sequence YECX<sub>2</sub>CX<sub>3</sub>FX<sub>5</sub>LX<sub>2</sub>HX<sub>3</sub>HTGEKP, which is rather conservative especially in the link region between two zinc finger motifs (TGEKP) 展开更多
关键词 ZNF199)cDNA MORE Cloning of a novel human zinc finger protein GENE
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卵巢癌细胞系顺铂化疗敏感株与耐药株中ZNF217的表达 被引量:2
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作者 何春容 钟梅 孙爱民 《解放军医学杂志》 CAS CSCD 北大核心 2009年第8期980-982,共3页
目的探讨锌指蛋白(ZNF)217在卵巢癌细胞系顺铂敏感株与耐药株中表达的差异。方法选择6株卵巢癌细胞,其中A2780、SKOV-3和COC1为顺铂敏感株,A2780-DDP-R、SKOV-3-DDP-R和COC1-DDP-R为顺铂耐药株。ATP法检测细胞的相对发光单位(RLU),计算... 目的探讨锌指蛋白(ZNF)217在卵巢癌细胞系顺铂敏感株与耐药株中表达的差异。方法选择6株卵巢癌细胞,其中A2780、SKOV-3和COC1为顺铂敏感株,A2780-DDP-R、SKOV-3-DDP-R和COC1-DDP-R为顺铂耐药株。ATP法检测细胞的相对发光单位(RLU),计算顺铂对细胞的半效抑制浓度(IC50)和各耐药株的耐药指数(RI)。免疫荧光细胞化学法观察ZNF217在细胞内的定位,RT-PCR和Westernblotting法检测ZNF217mRNA和蛋白的表达水平。结果顺铂对A2780和A2780-DDP-R的IC50分别为18.1±2.3mg/L和47.9±3.8mg/L(P<0.01),对SKOV-3和SKOV-3-DDP-R为9.6±1.5mg/L和40.1±5.3mg/L(P<0.01),对COC1和COC1-DDP-R为4.8±0.9mg/L和15.3±2.8mg/L(P<0.01);A2780-DDP-R、SKOV-3-DDP-R、COC1-DDP-R对顺铂的RI分别为2.6、4.2和3.2。激光共聚焦显微镜可见,ZNF217蛋白在敏感株中主要分布于细胞质,在耐药株则主要分布于细胞核。耐药株中ZNF217mRNA和蛋白表达均明显高于敏感株。结论ZNF217的高表达和核转位可能与卵巢癌对顺铂耐药有关。 展开更多
关键词 znf217蛋白 卵巢肿瘤 顺铂 抗药性 肿瘤
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