To investigate immunoregulatory mechanisms of Sertoli cells in the testis in vitro and in vivo, we utilized our well-characterized Ureaplasma Urealyticum （UU）-induced model. We investigated the expressions of IL-1α...To investigate immunoregulatory mechanisms of Sertoli cells in the testis in vitro and in vivo, we utilized our well-characterized Ureaplasma Urealyticum （UU）-induced model. We investigated the expressions of IL-1α, IL-6, TGF-β, FasL and ZNF265 at the first, second and third weeks post-infection. During recovery from inflammation and with the help of negative regulators TGF-β and FasL, the high levels of IL-1α and IL-6 expressions were observed in the early stages of the infection, and decreased gradually in the later weeks both in vitro and in vivo. The trend of varied expression of ZNF265 was similar to those of TGF-β and FasL in vitro and in vivo for Sertoli cells infected with UU.展开更多
基金This study was financially supported by the key innovative project of the ShangHai educational committee （No. 09ZZ117） and Shanghai institute of Immunology Science Foundation （No. 08A05） We thank Professor Ping Xu （Institute of Life Science, Fu Dan University） for the gift of primary antibody, rabbit anti-rat ZNF265.
文摘To investigate immunoregulatory mechanisms of Sertoli cells in the testis in vitro and in vivo, we utilized our well-characterized Ureaplasma Urealyticum （UU）-induced model. We investigated the expressions of IL-1α, IL-6, TGF-β, FasL and ZNF265 at the first, second and third weeks post-infection. During recovery from inflammation and with the help of negative regulators TGF-β and FasL, the high levels of IL-1α and IL-6 expressions were observed in the early stages of the infection, and decreased gradually in the later weeks both in vitro and in vivo. The trend of varied expression of ZNF265 was similar to those of TGF-β and FasL in vitro and in vivo for Sertoli cells infected with UU.
