Objective Osteogenesis is vitally important for bone defect repair,and Zuo Gui Wan(ZGW)is a classic prescription in traditional Chinese medicine(TCM)for strengthening bones.However,the specific mechanism by which ZGW ...Objective Osteogenesis is vitally important for bone defect repair,and Zuo Gui Wan(ZGW)is a classic prescription in traditional Chinese medicine(TCM)for strengthening bones.However,the specific mechanism by which ZGW regulates osteogenesis is still unclear.The current study is based on a network pharmacology analysis to explore the potential mechanism of ZGW in promoting osteogenesis.Methods A network pharmacology analysis followed by experimental validation was applied to explore the potential mechanisms of ZGW in promoting the osteogenesis of bone marrow mesenchymal stem cells(BMSCs).Results In total,487 no-repeat targets corresponding to the bioactive components of ZGW were screened,and 175 target genes in the intersection of ZGW and osteogenesis were obtained.And 28 core target genes were then obtained from a PPI network analysis.A GO functional enrichment analysis showed that the relevant biological processes mainly involve the cellular response to chemical stress,metal ions,and lipopolysaccharide.Additionally,KEGG pathway enrichment analysis revealed that multiple signaling pathways,including the phosphatidylinositol-3-kinase/protein kinase B(PI3K/AKT)signaling pathway,were associated with ZGW-promoted osteogensis.Further experimental validation showed that ZGW could increase alkaline phosphatase(ALP)activity as well as the mRNA and protein levels of ALP,osteocalcin(OCN),and runt related transcription factor 2(Runx 2).What’s more,Western blot analysis results showed that ZGW significantly increased the protein levels of p-PI3K and p-AKT,and the increases of these protein levels significantly receded after the addition of the PI3K inhibitor LY294002.Finally,the upregulated osteogenic-related indicators were also suppressed by the addition of LY294002.Conclusion ZGW promotes the osteogenesis of BMSCs via PI3K/AKT signaling pathway.展开更多
名词短语作为重要词语组合之一,在神经科学、心理学、计算机科学等领域受到广泛关注,在语言学研究领域更是持续的热点,国外相关研究从1945年以来形成了气候,但是却没有相关研究综述。因此,以Web of Science核心集为数据来源,运用文献计...名词短语作为重要词语组合之一,在神经科学、心理学、计算机科学等领域受到广泛关注,在语言学研究领域更是持续的热点,国外相关研究从1945年以来形成了气候,但是却没有相关研究综述。因此,以Web of Science核心集为数据来源,运用文献计量工具CiteSpace,从文献作者、研究机构、研究热点和前沿等方面,对1945-2022年期间关于名词短语研究的文献进行了可视化分析。研究表明:(1)国外名词短语研究广泛且分散,发文国家多,仅形成美国、英国和法国三个高中介中心型国家。中国起步晚,但发展迅速,已成为发文量前五国家之一;(2) Schriefers、Caramazza和Costa发文量多且已形成合作网络。中国作者中香港城市大学兰舸形成了一定的合作网络。Chomsky和Levelt文献影响力最大;(3)名词短语生成、词序、一致性、搭配、名词短语复杂性是名词短语研究的热门话题。高被引文献主题集中在名词短语生成和名词短语复杂性两个方面,代表名词短语研究前沿。展开更多
文摘Objective Osteogenesis is vitally important for bone defect repair,and Zuo Gui Wan(ZGW)is a classic prescription in traditional Chinese medicine(TCM)for strengthening bones.However,the specific mechanism by which ZGW regulates osteogenesis is still unclear.The current study is based on a network pharmacology analysis to explore the potential mechanism of ZGW in promoting osteogenesis.Methods A network pharmacology analysis followed by experimental validation was applied to explore the potential mechanisms of ZGW in promoting the osteogenesis of bone marrow mesenchymal stem cells(BMSCs).Results In total,487 no-repeat targets corresponding to the bioactive components of ZGW were screened,and 175 target genes in the intersection of ZGW and osteogenesis were obtained.And 28 core target genes were then obtained from a PPI network analysis.A GO functional enrichment analysis showed that the relevant biological processes mainly involve the cellular response to chemical stress,metal ions,and lipopolysaccharide.Additionally,KEGG pathway enrichment analysis revealed that multiple signaling pathways,including the phosphatidylinositol-3-kinase/protein kinase B(PI3K/AKT)signaling pathway,were associated with ZGW-promoted osteogensis.Further experimental validation showed that ZGW could increase alkaline phosphatase(ALP)activity as well as the mRNA and protein levels of ALP,osteocalcin(OCN),and runt related transcription factor 2(Runx 2).What’s more,Western blot analysis results showed that ZGW significantly increased the protein levels of p-PI3K and p-AKT,and the increases of these protein levels significantly receded after the addition of the PI3K inhibitor LY294002.Finally,the upregulated osteogenic-related indicators were also suppressed by the addition of LY294002.Conclusion ZGW promotes the osteogenesis of BMSCs via PI3K/AKT signaling pathway.
文摘名词短语作为重要词语组合之一,在神经科学、心理学、计算机科学等领域受到广泛关注,在语言学研究领域更是持续的热点,国外相关研究从1945年以来形成了气候,但是却没有相关研究综述。因此,以Web of Science核心集为数据来源,运用文献计量工具CiteSpace,从文献作者、研究机构、研究热点和前沿等方面,对1945-2022年期间关于名词短语研究的文献进行了可视化分析。研究表明:(1)国外名词短语研究广泛且分散,发文国家多,仅形成美国、英国和法国三个高中介中心型国家。中国起步晚,但发展迅速,已成为发文量前五国家之一;(2) Schriefers、Caramazza和Costa发文量多且已形成合作网络。中国作者中香港城市大学兰舸形成了一定的合作网络。Chomsky和Levelt文献影响力最大;(3)名词短语生成、词序、一致性、搭配、名词短语复杂性是名词短语研究的热门话题。高被引文献主题集中在名词短语生成和名词短语复杂性两个方面,代表名词短语研究前沿。
