[Objective] The paper was to identify stain ZY-19-2 with inhibitory effect against tobacco black shank （Phytophtora parasitica var.nicotianae Tucker）, and study the fermentation condition of the strain. [Method]A st...[Objective] The paper was to identify stain ZY-19-2 with inhibitory effect against tobacco black shank （Phytophtora parasitica var.nicotianae Tucker）, and study the fermentation condition of the strain. [Method]A strain ZY-19-2 with strong inhibitory effect against P. parasitica were isolated and screened from tobacco rhizosphere soil samples, and identified according to its morphological characteristics. The chitinase production activity of the strain under different culture conditions was also studied. [Result] For stain ZY-19-2 Paecilomyces lilacinus, the optimal fermentation conditions were as follows： 1.2% colloidal chitin as carbon source, 1% peptone as nitrogen source, 0.1% Tween 80 as surfactant, initial pH of fermentation broth at 6.0, the fermentation time of 60 h, inoculum amount at 1%, shaker speed at 120 r/min. The highest enzyme activity reached 0.216 U/ml. [Conclusion]The optimization of fermentation condition of strain ZY-19-2 lay foundation for large-scale production of cheap and efficient chitinase and chitin oligosaccharides, as well as application of the strain for control of tobacco black shank.展开更多
China successfully put its earth resource satellite, the third of ZY-2, into the orbit aboard a LM-4B launch vehicle that blasted off at 11:10 am on Nov. 6, 2004 from Taiyuan Satellite Launch Center in Shanxi Province.
Block Adjustment(BA)is one of the essential techniques for producing high-precision geospatial 3D data products with optical stereo satellite imagery.For block adjustment with few ground-control points or without grou...Block Adjustment(BA)is one of the essential techniques for producing high-precision geospatial 3D data products with optical stereo satellite imagery.For block adjustment with few ground-control points or without ground control,the vertical error of the model is the decisive factor that constrains the accuracy of 3D data products.The elevation data obtained by spaceborne laser altimeter have the advantages of short update periods,high positioning precision,and low acquisition cost,providing sufficient data support for improving the elevation accuracy of stereo models through the combined BA.This paper proposes a geometric positioning model based on the integration of Optical Satellite Stereo Imagery(OSSI)and spaceborne laser altimeter data.Firstly,we elaborate the principle and necessity of this work through a literature review of existing methods.Then,the framework of our geo-positioning models.Secondly,four key technologies of the proposed model are expounded in order,including the acquisition and management of global Laser Control Points,the association of LCPs and OSSI,the block adjustment model combining LCPs with OSSI,and the accuracy estimation and quality control of the combined BA.Next,the combined BA experiment using Ziyuan-3(ZY-3)OSSI and ICESat-2 laser data was carried out at the testing site in Shandong Province,China.Experimental results prove that our method can automatically select LCPs with high accuracy.The elevation deviation of the combined BA eventually achieved the Mean Error(ME)of 0.06 m and the Root Mean Square Error(RMSE)of 1.18 m,much lower than the ME of 13.20 m and the RMSE of 3.88 m before the block adjustment.A further research direction will be how to perform more adequate accuracy analysis and quality control using massive laser points as checkpoints.展开更多
基金Supported by Technology Development Project of Zhengzhou Tobacco Research Institute of CNTC"Isolation,Application and Research of Disease-resistant Endophyte"(122009CZ0420)~~
文摘[Objective] The paper was to identify stain ZY-19-2 with inhibitory effect against tobacco black shank （Phytophtora parasitica var.nicotianae Tucker）, and study the fermentation condition of the strain. [Method]A strain ZY-19-2 with strong inhibitory effect against P. parasitica were isolated and screened from tobacco rhizosphere soil samples, and identified according to its morphological characteristics. The chitinase production activity of the strain under different culture conditions was also studied. [Result] For stain ZY-19-2 Paecilomyces lilacinus, the optimal fermentation conditions were as follows： 1.2% colloidal chitin as carbon source, 1% peptone as nitrogen source, 0.1% Tween 80 as surfactant, initial pH of fermentation broth at 6.0, the fermentation time of 60 h, inoculum amount at 1%, shaker speed at 120 r/min. The highest enzyme activity reached 0.216 U/ml. [Conclusion]The optimization of fermentation condition of strain ZY-19-2 lay foundation for large-scale production of cheap and efficient chitinase and chitin oligosaccharides, as well as application of the strain for control of tobacco black shank.
文摘China successfully put its earth resource satellite, the third of ZY-2, into the orbit aboard a LM-4B launch vehicle that blasted off at 11:10 am on Nov. 6, 2004 from Taiyuan Satellite Launch Center in Shanxi Province.
基金supported by the National Science Fund for Distinguished Young Scholars[grant number 61825103]the Fundamental Research Funds for The Central Universities[grant number 2042022kf1002].
文摘Block Adjustment(BA)is one of the essential techniques for producing high-precision geospatial 3D data products with optical stereo satellite imagery.For block adjustment with few ground-control points or without ground control,the vertical error of the model is the decisive factor that constrains the accuracy of 3D data products.The elevation data obtained by spaceborne laser altimeter have the advantages of short update periods,high positioning precision,and low acquisition cost,providing sufficient data support for improving the elevation accuracy of stereo models through the combined BA.This paper proposes a geometric positioning model based on the integration of Optical Satellite Stereo Imagery(OSSI)and spaceborne laser altimeter data.Firstly,we elaborate the principle and necessity of this work through a literature review of existing methods.Then,the framework of our geo-positioning models.Secondly,four key technologies of the proposed model are expounded in order,including the acquisition and management of global Laser Control Points,the association of LCPs and OSSI,the block adjustment model combining LCPs with OSSI,and the accuracy estimation and quality control of the combined BA.Next,the combined BA experiment using Ziyuan-3(ZY-3)OSSI and ICESat-2 laser data was carried out at the testing site in Shandong Province,China.Experimental results prove that our method can automatically select LCPs with high accuracy.The elevation deviation of the combined BA eventually achieved the Mean Error(ME)of 0.06 m and the Root Mean Square Error(RMSE)of 1.18 m,much lower than the ME of 13.20 m and the RMSE of 3.88 m before the block adjustment.A further research direction will be how to perform more adequate accuracy analysis and quality control using massive laser points as checkpoints.
