Information regarding immunocompetence of the adaptive immune system(AIS) in zebrafish Danio rerio remains limited.Here,we stimulated an immune response in fish embryos,larvae and adults using lipopolysaccharide(LPS) ...Information regarding immunocompetence of the adaptive immune system(AIS) in zebrafish Danio rerio remains limited.Here,we stimulated an immune response in fish embryos,larvae and adults using lipopolysaccharide(LPS) and measured the upregulation of a number of AIS-related genes(Rag2,AID,TCRAC,IgLC-1,mIg,sIg,IgZ and DAB) 3 and 18 h later.We found that all of the genes evaluated were strongly induced following LPS stimulation,with most of them responding at 8 d post fertilization.This confirms that a functional adaptive immune response is present in D.rerio larvae,and provides a window for further functional analyses.展开更多
Cadmium (Cd),a ubiquitous environmental hazardous heavy metal,poses a significant threat to the health of aquatic organisms,including teleosts.Although the toxic profile of Cd is well recognized,little is known regard...Cadmium (Cd),a ubiquitous environmental hazardous heavy metal,poses a significant threat to the health of aquatic organisms,including teleosts.Although the toxic profile of Cd is well recognized,little is known regarding the overall view of toxic responses to varying aquatic environmental parameters (e.g.,water hardness) at an individual level.Herein,differences in water hardness were partially mimicked by adjusting Ca^(2+)levels in E3 medium.As an in vivo model,zebrafish embryos were exposed to variable Ca^(2+)levels (NV,normal Ca^(2+);LV,low Ca^(2+);HV,high Ca^(2+)) alone or combined with 30.7μg/L Cd^(2+)(NC,LC,and HC,respectively) until 144 hr post-fertilization.The genome-wide transcriptome revealed differentially expressed genes between groups.Functional enrichment analysis found that biological processes related to metabolism,particularly lipid metabolism,were significantly disrupted in NC and LC treatments,while a remission was observed in the HC group.Biochemical assays confirmed that the decrease in Ca^(2+)enhanced synthesis,inhibited mobilization and increased the storage of lipids in Cd^(2+)treatments.This study suggests that the toxic effect of Cd on biological pathways will be influenced by Ca^(2+),which will improve the toxicological understanding and facilitate accurate assessment of Cd.展开更多
The use of exogenous carbon monoxide releasing molecules（CORMs）provides promise for clinical application;however,the hazard potential of CORMs in vivo remains poorly understood.The developmental toxicity of CORM-3 w...The use of exogenous carbon monoxide releasing molecules（CORMs）provides promise for clinical application;however,the hazard potential of CORMs in vivo remains poorly understood.The developmental toxicity of CORM-3 was investigated by exposure to concentrations ranging from 6.25 to400μmol/L during 4-144 h post fertilization.Toxicity endpoints of mortality,spontaneous movement,heart rate,hatching rate,malformation,body length,and larval behavior were measured.展开更多
OBJECTIVE:To evaluate the effects of Rongchang capsule and Xifeng capsule on pentylenetetrazoleinduced epilepsy in zebrafish larvae and to explore the possible mechanisms behind their actions.METHODS:We utilized a tra...OBJECTIVE:To evaluate the effects of Rongchang capsule and Xifeng capsule on pentylenetetrazoleinduced epilepsy in zebrafish larvae and to explore the possible mechanisms behind their actions.METHODS:We utilized a trajectory tracking system to monitor seizures in zebrafish larva to confirm that certain concentrations of Rongchang capsule and Xifeng capsule produce antiepileptic effects.c-fos expression was assessed by quantitative reverse transcription-polymerase chain reaction to validate the efficacy of the capsules.Rest/wake behavior and correlation analysis predicted the targets of Rongchang capsule and Xifeng capsule.RESULTS:Larval movement times and total distances traveled by zebrafish larvae experiencing pentylenetetrazole(PTZ)-induced seizures were decreasedbyvalproatetreatment.Rongchang(500μg/m L)and Xifeng(200μg/m L)rescued the epileptic behaviors and down-regulated c-fos expression in the brains of larvae,which indicated antiepileptic effects.The rest/wake behavioral profiles showed that Rongchang and Xifeng differentially decreased rest time at night and increased larval locomotor activities during the day.Based on correlation between the actions of the two capsules and known compounds,we predicted that they might change rest/wake behaviors by affecting serotonin,GABAergic and histamine signaling pathways.CONCLUSION:The efficacy of Rongchang capsule and Xifeng capsule in alleviating epilepsy-like behaviors and molecular responses was confirmed.Our study provides insight into the capsules'effect on epilepsy.展开更多
基金Supported by the Ministry of Science and Technology (MOST) of China (No. 2008AA09Z409)
文摘Information regarding immunocompetence of the adaptive immune system(AIS) in zebrafish Danio rerio remains limited.Here,we stimulated an immune response in fish embryos,larvae and adults using lipopolysaccharide(LPS) and measured the upregulation of a number of AIS-related genes(Rag2,AID,TCRAC,IgLC-1,mIg,sIg,IgZ and DAB) 3 and 18 h later.We found that all of the genes evaluated were strongly induced following LPS stimulation,with most of them responding at 8 d post fertilization.This confirms that a functional adaptive immune response is present in D.rerio larvae,and provides a window for further functional analyses.
基金supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (No. XDA23040401)the National Natural Science Foundation of China (No. 21806091)the Natural Science Foundation of Hubei Province (No. 2020CFB154)。
文摘Cadmium (Cd),a ubiquitous environmental hazardous heavy metal,poses a significant threat to the health of aquatic organisms,including teleosts.Although the toxic profile of Cd is well recognized,little is known regarding the overall view of toxic responses to varying aquatic environmental parameters (e.g.,water hardness) at an individual level.Herein,differences in water hardness were partially mimicked by adjusting Ca^(2+)levels in E3 medium.As an in vivo model,zebrafish embryos were exposed to variable Ca^(2+)levels (NV,normal Ca^(2+);LV,low Ca^(2+);HV,high Ca^(2+)) alone or combined with 30.7μg/L Cd^(2+)(NC,LC,and HC,respectively) until 144 hr post-fertilization.The genome-wide transcriptome revealed differentially expressed genes between groups.Functional enrichment analysis found that biological processes related to metabolism,particularly lipid metabolism,were significantly disrupted in NC and LC treatments,while a remission was observed in the HC group.Biochemical assays confirmed that the decrease in Ca^(2+)enhanced synthesis,inhibited mobilization and increased the storage of lipids in Cd^(2+)treatments.This study suggests that the toxic effect of Cd on biological pathways will be influenced by Ca^(2+),which will improve the toxicological understanding and facilitate accurate assessment of Cd.
基金supported by National Basic Research Program of China(2010CB834202)the National Natural Science Foundation of China(11305226,11175222)+1 种基金the Scientific Technology Research Projects of Lanzhou City(2015-3-75)the Western Talent Program of Chinese Academy of Sciences(Y460030XB0)
文摘The use of exogenous carbon monoxide releasing molecules（CORMs）provides promise for clinical application;however,the hazard potential of CORMs in vivo remains poorly understood.The developmental toxicity of CORM-3 was investigated by exposure to concentrations ranging from 6.25 to400μmol/L during 4-144 h post fertilization.Toxicity endpoints of mortality,spontaneous movement,heart rate,hatching rate,malformation,body length,and larval behavior were measured.
基金Supported by the Chinese National Natural Science Foundation of China:In situ microscopic Analysis and manipulator for Life Science(No.61327802)the Chinese National Natural Science Foundation of China:Functional Nanoparticle Drug Vector for Zebrafish Development and Biocompatible Assessmen(No.81501589)+3 种基金the Chinese National Natural Science Foundation of China:Development of a cross-scale fast AFM system for life science(No.61127006)the Chinese National Natural Science Foundation of China:Study on the Method of Automatic Nano-manipulation for Cell-Oriented Accurate Displacement(No.61633012)the Tianjin Science Technology Research Funds of China:the function of Rab23 gene in zebrafish development(No.14JCQNJC09600)the National Basic Research Program of China:The Basic and Frontier of the New Topological Structure of the Molecular-based Functional Carbon Materials(No.2015CB856500)
文摘OBJECTIVE:To evaluate the effects of Rongchang capsule and Xifeng capsule on pentylenetetrazoleinduced epilepsy in zebrafish larvae and to explore the possible mechanisms behind their actions.METHODS:We utilized a trajectory tracking system to monitor seizures in zebrafish larva to confirm that certain concentrations of Rongchang capsule and Xifeng capsule produce antiepileptic effects.c-fos expression was assessed by quantitative reverse transcription-polymerase chain reaction to validate the efficacy of the capsules.Rest/wake behavior and correlation analysis predicted the targets of Rongchang capsule and Xifeng capsule.RESULTS:Larval movement times and total distances traveled by zebrafish larvae experiencing pentylenetetrazole(PTZ)-induced seizures were decreasedbyvalproatetreatment.Rongchang(500μg/m L)and Xifeng(200μg/m L)rescued the epileptic behaviors and down-regulated c-fos expression in the brains of larvae,which indicated antiepileptic effects.The rest/wake behavioral profiles showed that Rongchang and Xifeng differentially decreased rest time at night and increased larval locomotor activities during the day.Based on correlation between the actions of the two capsules and known compounds,we predicted that they might change rest/wake behaviors by affecting serotonin,GABAergic and histamine signaling pathways.CONCLUSION:The efficacy of Rongchang capsule and Xifeng capsule in alleviating epilepsy-like behaviors and molecular responses was confirmed.Our study provides insight into the capsules'effect on epilepsy.
基金Acknowledgments The authors thank the National Natural Science Foundation of China （81302646）, Natural Science Foundation of Zhejiang Province （LQ13H300002）, Science Technology Department of Zhejiang Province （2015F50015） and Health and Family Planning commission of Zhejiang Province （XKQ-010-001 and 2013KYB070） for financial support.
