We analyzed the fine-scale spatial genetic structure of the individuals of Zelkova schneideriana , which were classified by age using the spatial autocorrelation method, to quantify spatial patterns of genetic variati...We analyzed the fine-scale spatial genetic structure of the individuals of Zelkova schneideriana , which were classified by age using the spatial autocorrelation method, to quantify spatial patterns of genetic variation within the population and to explore potential mechanisms that determine genetic variation in population. The spatial autocorrelation coefficient （ r ） at 13 distance classes was determined on the basis of both geographical distance and genetic distance matrix which was derived from co-dominant SSR data using GenAlEx software. The results showed that all the individuals of Z. schneideriana exhibited significantly positive spatial genetic structure at distance less than 40 m （the X -intercept was 53.568）, indicating that the average length of the smallest genetic patch for the same genotype clustering of the Z. schneideriana Mailing population was about 50 m. Limited seed dispersal is the main factor that leads to the spatial genetic variation within populations. The individuals in age Class II showed significantly positive spatial genetic structure at distance less than 30 m （the X -intercept was 47.882）, while the individuals in age Class I and age Class III showed no significant spatial genetic structure in any of the spatial distance classes. Z. schneideriana is a long-lived perennial plant; the self-thinning resulted from the cohort competition between individuals in the growing process may lead to this certain spatial structure in age Class III of Z. schneideriana population.展开更多
应用ISSR(Inter S imp le Sequence Repeat)分子标记技术对云南邱北、易门、龙庆、双柏和浙江平湖5个种源(每种源19个样本)的榉树进行了遗传多样性的研究。由其18条ISSR引物扩增得到216个清晰的条带,其中多态性条带215个,多态性条带百分...应用ISSR(Inter S imp le Sequence Repeat)分子标记技术对云南邱北、易门、龙庆、双柏和浙江平湖5个种源(每种源19个样本)的榉树进行了遗传多样性的研究。由其18条ISSR引物扩增得到216个清晰的条带,其中多态性条带215个,多态性条带百分率(PPB)为99.54%。POPGENE软件分析结果表明,邱北种群的遗传多样性水平最高(PPB=88.43%,HE=0.260 5,HO=0.401 4),其次是易门种群,龙庆、双柏、平湖3个种群的遗传多样性水平相差较小。Ne i’s遗传多样性的分析表明,5个榉树种源在其总的遗传变异中有80.28%的存在于群体内,群体间的遗传变异仅占总变异的19.72%。由UPGMA聚类分析可知,易门和龙庆种群的亲缘关系最近,先聚合在一起,然后依次与邱北、双柏聚类,最后与亲缘关系最远的平湖种群聚合。其研究结果对榉树资源的遗传多样性保护具有指导作用。展开更多
基金Supported by the Scientific Research and Technological Development Project of Guangxi(1598025-42)the Guangxi Youth Fund Project(2013GXNSFBA019093)+1 种基金the National Forestry Public Welfare Industry Research Project(200904011)the Open Project for Guangxi Key Laboratory of Superior Timber Trees Resource Cultivation(15-B-03-01)
文摘We analyzed the fine-scale spatial genetic structure of the individuals of Zelkova schneideriana , which were classified by age using the spatial autocorrelation method, to quantify spatial patterns of genetic variation within the population and to explore potential mechanisms that determine genetic variation in population. The spatial autocorrelation coefficient （ r ） at 13 distance classes was determined on the basis of both geographical distance and genetic distance matrix which was derived from co-dominant SSR data using GenAlEx software. The results showed that all the individuals of Z. schneideriana exhibited significantly positive spatial genetic structure at distance less than 40 m （the X -intercept was 53.568）, indicating that the average length of the smallest genetic patch for the same genotype clustering of the Z. schneideriana Mailing population was about 50 m. Limited seed dispersal is the main factor that leads to the spatial genetic variation within populations. The individuals in age Class II showed significantly positive spatial genetic structure at distance less than 30 m （the X -intercept was 47.882）, while the individuals in age Class I and age Class III showed no significant spatial genetic structure in any of the spatial distance classes. Z. schneideriana is a long-lived perennial plant; the self-thinning resulted from the cohort competition between individuals in the growing process may lead to this certain spatial structure in age Class III of Z. schneideriana population.
文摘应用ISSR(Inter S imp le Sequence Repeat)分子标记技术对云南邱北、易门、龙庆、双柏和浙江平湖5个种源(每种源19个样本)的榉树进行了遗传多样性的研究。由其18条ISSR引物扩增得到216个清晰的条带,其中多态性条带215个,多态性条带百分率(PPB)为99.54%。POPGENE软件分析结果表明,邱北种群的遗传多样性水平最高(PPB=88.43%,HE=0.260 5,HO=0.401 4),其次是易门种群,龙庆、双柏、平湖3个种群的遗传多样性水平相差较小。Ne i’s遗传多样性的分析表明,5个榉树种源在其总的遗传变异中有80.28%的存在于群体内,群体间的遗传变异仅占总变异的19.72%。由UPGMA聚类分析可知,易门和龙庆种群的亲缘关系最近,先聚合在一起,然后依次与邱北、双柏聚类,最后与亲缘关系最远的平湖种群聚合。其研究结果对榉树资源的遗传多样性保护具有指导作用。
