This paper reports the histological observation of larvae ofZethenia rufescentaria Motsch. after infection by ZrNPV. Histopathologic study revealed that ZrNPV were multiplied within the nuclear of fat body, epidermis ...This paper reports the histological observation of larvae ofZethenia rufescentaria Motsch. after infection by ZrNPV. Histopathologic study revealed that ZrNPV were multiplied within the nuclear of fat body, epidermis cell, midgut cell, tracheal matrix and blood cell. These cells showed obvious cytopathic effects. The nucleus of infected cells underwent swelled. Under electron microscope, virus and polyhedral of ZrNPV were clearly observed in these nucleus of infected cells. The nucleus of susceptible tissues were fulfilled with polyhedra after 70–140 h.展开更多
Zethenia rufescentaria Motsch. has outbroken successively in the larch plantation of Jixi region and seriously damaged stands since 1990. Chemical control was studied for controlling the occurrence of the pest. The r...Zethenia rufescentaria Motsch. has outbroken successively in the larch plantation of Jixi region and seriously damaged stands since 1990. Chemical control was studied for controlling the occurrence of the pest. The results showed as follows: When the third or the forth instar larvae was controlled with smoke agent of 5% Lindane, the most suitable time was July 10-13. The area controlled was 2000 hm2. The dosage was 7.5 kg/hm2 and the mortality of the pest reached 93.5%. 25%dimilin Ⅲ (flowable formulation) was used to prevent the third instar larvae. The best time was about July 5. The dosage was 150g/hm2. The mortality of the pest reached 89.85%. 3% Hexyclan dusting was also used to control adults of the pest. The best time was before the peak of their emergence (June 3). the dosage was 11.3 kg/hm2. The mortality of the pests reached 89.6%.展开更多
文摘This paper reports the histological observation of larvae ofZethenia rufescentaria Motsch. after infection by ZrNPV. Histopathologic study revealed that ZrNPV were multiplied within the nuclear of fat body, epidermis cell, midgut cell, tracheal matrix and blood cell. These cells showed obvious cytopathic effects. The nucleus of infected cells underwent swelled. Under electron microscope, virus and polyhedral of ZrNPV were clearly observed in these nucleus of infected cells. The nucleus of susceptible tissues were fulfilled with polyhedra after 70–140 h.
文摘Zethenia rufescentaria Motsch. has outbroken successively in the larch plantation of Jixi region and seriously damaged stands since 1990. Chemical control was studied for controlling the occurrence of the pest. The results showed as follows: When the third or the forth instar larvae was controlled with smoke agent of 5% Lindane, the most suitable time was July 10-13. The area controlled was 2000 hm2. The dosage was 7.5 kg/hm2 and the mortality of the pest reached 93.5%. 25%dimilin Ⅲ (flowable formulation) was used to prevent the third instar larvae. The best time was about July 5. The dosage was 150g/hm2. The mortality of the pest reached 89.85%. 3% Hexyclan dusting was also used to control adults of the pest. The best time was before the peak of their emergence (June 3). the dosage was 11.3 kg/hm2. The mortality of the pests reached 89.6%.
文摘通过对接种 ZrNPV 后落叶松绶尺蠖幼虫的各种组织进行光学显微镜和电子显微镜观察、发现落叶松绶尺蠖核型多角体病毒在脂肪体,表皮细胞,中肠细胞,气管基质和血细胞内复制。上述细胞受感染后均出现明显的病理变化,细胞核增大。在电镜下可观察到受感染组织的细胞核内出现病毒粒子和多角体,70～140 h 所有受感染组织核内充满多角体。
