The water-soluble part（GS） of Ganoderma sinense Zhao, Xu et Zhang was divided into high molecular（GS-H） and low molecular（GS-L） parts by Cellulose Super Filtration, and GS was also fractionated into four fractio...The water-soluble part（GS） of Ganoderma sinense Zhao, Xu et Zhang was divided into high molecular（GS-H） and low molecular（GS-L） parts by Cellulose Super Filtration, and GS was also fractionated into four fractions, GS-1, 2, 3, and 4 by ethanol precipitation according to their molecular weights. Chemical analysis shows that GS and GS-1, 2, 3, 4 were complexes of polysaccharide and peptide. The fractions with molecular weights over 4000, GS-1, 2, 3, and GS-H show anti-tumor activities, however, the fractions with molecular weights lower than 4000, GS-4, and GS-L have no anti-tumor activity, indicating that the anti-tumor activity of Ganoderma Sinensis was caused by glucopeptides with molecular weight ranging from 4000 to 20000. Two purified glucopeptides, GS-6b and GS-7b were obtained from GS-H by ion-exchange and gel-permeation chromatography. Their molecular weights, glycosidic linkages, and configurations were detected by means of IR spectrum, sugar composition analysis, and methylation analysis. The polysaccharide parts of GS-6b and GS-7b had glucan backbone consisting of β-1→3 Glc, and side chain containing glucosyl, mannosyl, fucosyl, xylosyl, galactosyl, and glucuronic acid residues attached on 1-2, 1-4, 1-6 positions of the backbone of GS-6b, or 1-6, 1-4 positions of the backbone of GS-7b. The peptide parts in GS-6b and GS-7b were composed of 10 kinds of amino acids, including Asp, Ser, Arg, Gly, Thr, Pro, Ala, Val, Met, and Lys.展开更多
The World Journal of Gastrointestinal Oncology Editorial Board consists of 404 members,representing a team of worldwide experts in gastrointestinal oncology.They are from 41 countries,including Argentina(1),Australia(9),
The contributions of referees continue to be essential to the maintenance of the quality of the papers published in Geoscience Frontiers. Their critiques and hard work in reviewing, and sometimes, re-reviewing manuscr...The contributions of referees continue to be essential to the maintenance of the quality of the papers published in Geoscience Frontiers. Their critiques and hard work in reviewing, and sometimes, re-reviewing manuscripts submitted to the journal are highly appreciated by the Editors, GSF Editorial Office staff and Publisher. We wish to use this opportunity to thank all the following referees of manuscripts submitted in 2010 and 2011.展开更多
The World Journal of Radiology Editorial Board consists of 365 members,representing a team of worldwide experts in radiology.They are from 36 countries,including Afghanistan(1),Argentina(2),Australia(5),Austria(7),Bel...The World Journal of Radiology Editorial Board consists of 365 members,representing a team of worldwide experts in radiology.They are from 36 countries,including Afghanistan(1),Argentina(2),Australia(5),Austria(7),Belgium(2),Brazil(8),Canada(6),Chile(1),China(43),Croatia(1),Denmark(4),Egypt(6),France(5),Germany(22)。展开更多
文摘The water-soluble part（GS） of Ganoderma sinense Zhao, Xu et Zhang was divided into high molecular（GS-H） and low molecular（GS-L） parts by Cellulose Super Filtration, and GS was also fractionated into four fractions, GS-1, 2, 3, and 4 by ethanol precipitation according to their molecular weights. Chemical analysis shows that GS and GS-1, 2, 3, 4 were complexes of polysaccharide and peptide. The fractions with molecular weights over 4000, GS-1, 2, 3, and GS-H show anti-tumor activities, however, the fractions with molecular weights lower than 4000, GS-4, and GS-L have no anti-tumor activity, indicating that the anti-tumor activity of Ganoderma Sinensis was caused by glucopeptides with molecular weight ranging from 4000 to 20000. Two purified glucopeptides, GS-6b and GS-7b were obtained from GS-H by ion-exchange and gel-permeation chromatography. Their molecular weights, glycosidic linkages, and configurations were detected by means of IR spectrum, sugar composition analysis, and methylation analysis. The polysaccharide parts of GS-6b and GS-7b had glucan backbone consisting of β-1→3 Glc, and side chain containing glucosyl, mannosyl, fucosyl, xylosyl, galactosyl, and glucuronic acid residues attached on 1-2, 1-4, 1-6 positions of the backbone of GS-6b, or 1-6, 1-4 positions of the backbone of GS-7b. The peptide parts in GS-6b and GS-7b were composed of 10 kinds of amino acids, including Asp, Ser, Arg, Gly, Thr, Pro, Ala, Val, Met, and Lys.
文摘The World Journal of Gastrointestinal Oncology Editorial Board consists of 404 members,representing a team of worldwide experts in gastrointestinal oncology.They are from 41 countries,including Argentina(1),Australia(9),
文摘The contributions of referees continue to be essential to the maintenance of the quality of the papers published in Geoscience Frontiers. Their critiques and hard work in reviewing, and sometimes, re-reviewing manuscripts submitted to the journal are highly appreciated by the Editors, GSF Editorial Office staff and Publisher. We wish to use this opportunity to thank all the following referees of manuscripts submitted in 2010 and 2011.
文摘The World Journal of Radiology Editorial Board consists of 365 members,representing a team of worldwide experts in radiology.They are from 36 countries,including Afghanistan(1),Argentina(2),Australia(5),Austria(7),Belgium(2),Brazil(8),Canada(6),Chile(1),China(43),Croatia(1),Denmark(4),Egypt(6),France(5),Germany(22)。
