Objective： To investigate the effect of the Chinese medical formula Qubi Zhentong Recipe （祛痹镇痛方 QZR） on the synovial gene expression profile in collagen-induced arthritis （CIA） rats. Methods： Ten rats were ...Objective： To investigate the effect of the Chinese medical formula Qubi Zhentong Recipe （祛痹镇痛方 QZR） on the synovial gene expression profile in collagen-induced arthritis （CIA） rats. Methods： Ten rats were randomly chosen from 60 rats as the control group, and the other 50 rats were used for the CIA models. The CIA model group was constructed by bovine injection of type Ⅱ collagen through the rats＇ neck and tail. Twenty rats were randomly chosen from 34 successful CIA models and randomly assigned into two groups： the model group （n=10） and the QZR group （n=10）. The QZR group was fed intragastrically with QZR 22.9 g/（kg.d） （10 times the clinical adult dose）, and the CIA model group was given the same dose of normal saline. Both model and QZR groups were administered treatment once a day. Total RNA was collected from the knee joint synovium after 30 days. The change in gene expression profile was analyzed by a whole gene chip. Results： A total of 76 genes showed a difference in expression between CIA model group and the control group; 35 genes were down-regulated and 41 were up-regulated. A total of 67 genes showed a difference in expression between the model group and the QZR group; 48 genes were down-regulated and 19 were up- regulated. Conclusions： QZR may affect CIA by stimulating multiple genes and targets, which are related to oncogenes, apoptosis, metabolism, the immune system, ion channels, and transport proteins.展开更多
目的:基于网络药理学及分子对接挖掘散结镇痛胶囊治疗子宫内膜异位症的有效成分、作用靶点、信号通路以探索其分子生物学机制。方法:通过TCMSP数据库检索散结镇痛胶囊的有效成分;检索子宫内膜异位症疾病相关靶点,得到散结镇痛胶囊和子...目的:基于网络药理学及分子对接挖掘散结镇痛胶囊治疗子宫内膜异位症的有效成分、作用靶点、信号通路以探索其分子生物学机制。方法:通过TCMSP数据库检索散结镇痛胶囊的有效成分;检索子宫内膜异位症疾病相关靶点,得到散结镇痛胶囊和子宫内膜异位症的交集靶点;利用Cytoscape、STRING数据库构建蛋白互作用网络(protein-protein interaction,PPI)筛选核心蛋白;通过David数据库完成基因本体(gene ontology,GO)和京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)富集分析;利用AutoDock完成关键成分与靶点分子对接验证。结果:筛选出散结镇痛胶囊54个化合物和943个靶点,内膜异位症靶点1088个,获得两者交集基因231个,筛选获得VEGFA、ALB、Akt1、MAPK3、SRC等45个核心靶点。分子对接显示β-谷甾醇与AKT1、SRC,紫檀芪与Akt1、MAPK3分子结合更稳定。结论:散结镇痛胶囊可通过多成分、多靶点、多通路协同作用治疗子宫内膜异位症,机制可能为有效成分及靶点作用于HIF-1信号通路、雌激素信号通路、TNF信号通路有关。展开更多
基金Supported by the Zhejiang Provincial Project of Traditional Chinese Medicine Science and Technology Plan(No.2010ZB114)Hangzhou Financing Projects of Scientific Technology Development Program(No.20100733Q21)
文摘Objective： To investigate the effect of the Chinese medical formula Qubi Zhentong Recipe （祛痹镇痛方 QZR） on the synovial gene expression profile in collagen-induced arthritis （CIA） rats. Methods： Ten rats were randomly chosen from 60 rats as the control group, and the other 50 rats were used for the CIA models. The CIA model group was constructed by bovine injection of type Ⅱ collagen through the rats＇ neck and tail. Twenty rats were randomly chosen from 34 successful CIA models and randomly assigned into two groups： the model group （n=10） and the QZR group （n=10）. The QZR group was fed intragastrically with QZR 22.9 g/（kg.d） （10 times the clinical adult dose）, and the CIA model group was given the same dose of normal saline. Both model and QZR groups were administered treatment once a day. Total RNA was collected from the knee joint synovium after 30 days. The change in gene expression profile was analyzed by a whole gene chip. Results： A total of 76 genes showed a difference in expression between CIA model group and the control group; 35 genes were down-regulated and 41 were up-regulated. A total of 67 genes showed a difference in expression between the model group and the QZR group; 48 genes were down-regulated and 19 were up- regulated. Conclusions： QZR may affect CIA by stimulating multiple genes and targets, which are related to oncogenes, apoptosis, metabolism, the immune system, ion channels, and transport proteins.
文摘目的:基于网络药理学及分子对接挖掘散结镇痛胶囊治疗子宫内膜异位症的有效成分、作用靶点、信号通路以探索其分子生物学机制。方法:通过TCMSP数据库检索散结镇痛胶囊的有效成分;检索子宫内膜异位症疾病相关靶点,得到散结镇痛胶囊和子宫内膜异位症的交集靶点;利用Cytoscape、STRING数据库构建蛋白互作用网络(protein-protein interaction,PPI)筛选核心蛋白;通过David数据库完成基因本体(gene ontology,GO)和京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)富集分析;利用AutoDock完成关键成分与靶点分子对接验证。结果:筛选出散结镇痛胶囊54个化合物和943个靶点,内膜异位症靶点1088个,获得两者交集基因231个,筛选获得VEGFA、ALB、Akt1、MAPK3、SRC等45个核心靶点。分子对接显示β-谷甾醇与AKT1、SRC,紫檀芪与Akt1、MAPK3分子结合更稳定。结论:散结镇痛胶囊可通过多成分、多靶点、多通路协同作用治疗子宫内膜异位症,机制可能为有效成分及靶点作用于HIF-1信号通路、雌激素信号通路、TNF信号通路有关。
