[Objectives]To establish the TLC identification methods of Llicis Rotundae Cortex and Mahoniae Caulis and the determination method of syringin content in Zhuang Medicine Xiaoyan Zhiyang Lotion.[Methods]Thin layer chro...[Objectives]To establish the TLC identification methods of Llicis Rotundae Cortex and Mahoniae Caulis and the determination method of syringin content in Zhuang Medicine Xiaoyan Zhiyang Lotion.[Methods]Thin layer chromatography(TLC)was used to qualitatively identify Llicis Rotundae Cortex and Mahoniae Caulis in Zhuang Medicine Xiaoyan Zhiyang Lotion.The quantitative analysis of syringin in Zhuang Medicine Xiaoyan Zhiyang Lotion was carried out by high-performance liquid chromatography(HPLC).Chromatographic conditions are as follows:Luna 5μm C_(18)((2)100 A(250 mm×4.6 mm,5μm)column was adopted;acetonitrile(10%)-water(90%)was as the mobile phase,and the flow rate was 1.0 mL/min;the detection wavelength was 265 nm;the sample size was 5μL;the column temperature was 30℃.[Results]Syringin had a good linear relationship with its peak area in the range of 0.190-2.856μg(R^(2)=0.9995).The RSD values of precision,stability and repeatability experiments were all lower than 2.5%,and the average sampling recovery rate(n=6)was 100.37%(RSD=-0.88%).[Conclusions]The method had the advantages of simple operation,strong specificity,good repeatability and stability,and can be used for the quality control of Zhuang Medicine Xiaoyan Zhiyang Lotion.展开更多
目的采用超高效液相色谱（UPLC）方法,同时测定止痒洗剂中盐酸小檗碱、芍药苷和大黄素的含量。方法采用Aglient ZORBAX Eclipse Plus C18色谱柱（2.1×50 mm,1.8μm）,流动相为甲醇-0.1%磷酸水溶液,进行梯度洗脱,流速为0.4 m L·...目的采用超高效液相色谱（UPLC）方法,同时测定止痒洗剂中盐酸小檗碱、芍药苷和大黄素的含量。方法采用Aglient ZORBAX Eclipse Plus C18色谱柱（2.1×50 mm,1.8μm）,流动相为甲醇-0.1%磷酸水溶液,进行梯度洗脱,流速为0.4 m L·min-1,检测波长为240 nm,柱温为30℃。结果盐酸小檗碱、芍药苷和大黄素分别在0.0346-0.4152μg,0.3190-3.8280μg,0.0250-0.3000μg范围内呈良好的线性关系;平均回收率分别为96.44%,95.62%和96.48%。结论该方法简便、准确、重复性好,可为制定止痒洗剂质量标准及产品质量控制提供依据。展开更多
基金Supported by the Key R&D Project of Guangxi Science and Technology Department(Guike AB21196057)Construction Project for High-level Key Discipline of Traditional Chinese Medicine(Zhuang Pharmacy)of the National Administration of Traditional Chinese Medicine([2022]No.226)+3 种基金Scientific Research Project of Guangxi Medical Products Administration(Name:Safety Evaluation of Zhuang Medicine Anti-inflammation and Antipruritic Prescription for the Treatment of Skin Diseases Such as Acute Eczema and Contact Dermatitis)Interdisciplinary Innovation Team Project of Traditional Chinese Medicine in Guangxi(GZKJ2309)Natural Science Foundation Project of Guangxi University of Chinese Medicine in 2021(2021MS045)"High-level Talent Cultivation Innovation Team"Funding Project of Guangxi University of Chinese Medicine(2022A008).
文摘[Objectives]To establish the TLC identification methods of Llicis Rotundae Cortex and Mahoniae Caulis and the determination method of syringin content in Zhuang Medicine Xiaoyan Zhiyang Lotion.[Methods]Thin layer chromatography(TLC)was used to qualitatively identify Llicis Rotundae Cortex and Mahoniae Caulis in Zhuang Medicine Xiaoyan Zhiyang Lotion.The quantitative analysis of syringin in Zhuang Medicine Xiaoyan Zhiyang Lotion was carried out by high-performance liquid chromatography(HPLC).Chromatographic conditions are as follows:Luna 5μm C_(18)((2)100 A(250 mm×4.6 mm,5μm)column was adopted;acetonitrile(10%)-water(90%)was as the mobile phase,and the flow rate was 1.0 mL/min;the detection wavelength was 265 nm;the sample size was 5μL;the column temperature was 30℃.[Results]Syringin had a good linear relationship with its peak area in the range of 0.190-2.856μg(R^(2)=0.9995).The RSD values of precision,stability and repeatability experiments were all lower than 2.5%,and the average sampling recovery rate(n=6)was 100.37%(RSD=-0.88%).[Conclusions]The method had the advantages of simple operation,strong specificity,good repeatability and stability,and can be used for the quality control of Zhuang Medicine Xiaoyan Zhiyang Lotion.
文摘目的采用超高效液相色谱（UPLC）方法,同时测定止痒洗剂中盐酸小檗碱、芍药苷和大黄素的含量。方法采用Aglient ZORBAX Eclipse Plus C18色谱柱（2.1×50 mm,1.8μm）,流动相为甲醇-0.1%磷酸水溶液,进行梯度洗脱,流速为0.4 m L·min-1,检测波长为240 nm,柱温为30℃。结果盐酸小檗碱、芍药苷和大黄素分别在0.0346-0.4152μg,0.3190-3.8280μg,0.0250-0.3000μg范围内呈良好的线性关系;平均回收率分别为96.44%,95.62%和96.48%。结论该方法简便、准确、重复性好,可为制定止痒洗剂质量标准及产品质量控制提供依据。
