Zhumeria majdae is a medicinal plant and endemic of Iran that is being threatened to extinction. The present investigation to preserve this relict species, was conducted to study of: 1) different treatments effect on ...Zhumeria majdae is a medicinal plant and endemic of Iran that is being threatened to extinction. The present investigation to preserve this relict species, was conducted to study of: 1) different treatments effect on seeds germination: a) in vivo, b) in vitro were subjected to 5 treatments, 2) callus induction and organogenesis from leaf, root and stem of explants by tissue culture on MS medium supplemented with 7 different concentration and combinations of auxin and cytokinin. Results indicated the germination rate were promoted by seeds mucilage, and were inhibited under cold condition. The best callus induction were observed from stem and leaf explants in the MS + B(IAA(0.1) + Kin(1) mg·L-10). The maximum leaf formation of leaf explants were produced in the MS + E(IAA(0.1) + BA(2) mg·L-10).展开更多
Objective:To evaluate the effects of Zhumeria majdae essential oil(ZMEO)on morphine dependence and tolerance in mice.Methods:ZMEO(10,20,and 40 mg/kg)and clonidine(0.1 mg/kg)as the positive control were injected intrap...Objective:To evaluate the effects of Zhumeria majdae essential oil(ZMEO)on morphine dependence and tolerance in mice.Methods:ZMEO(10,20,and 40 mg/kg)and clonidine(0.1 mg/kg)as the positive control were injected intraperitoneally(i.p.).The effect of ZMEO and clonidine on the dependence were evaluated by counting the number of jumps induced by naloxone(5 mg/kg)while the tolerance was evaluated by the tail-flick test.Results:ZMEO at the dose of 10 mg/kg during the development period led to a significant inhibition of morphine tolerance(P<0.01),while it led to reduced morphine dependence with the doses of 20 and 40 mg/kg.ZMEO at two dose levels of 20 and 40 mg/kg indicated significant antinociceptive activity(P<0.01),and significantly reduced the withdrawal signs(number of jumps)of mice(P<0.01).Conclusions:ZMEO had significant effects on morphine tolerance and dependence.The linalool rich essential oil of Zmajdae plays a major role in the reduction of tolerance and dependence induced by morphine.展开更多
文摘Zhumeria majdae is a medicinal plant and endemic of Iran that is being threatened to extinction. The present investigation to preserve this relict species, was conducted to study of: 1) different treatments effect on seeds germination: a) in vivo, b) in vitro were subjected to 5 treatments, 2) callus induction and organogenesis from leaf, root and stem of explants by tissue culture on MS medium supplemented with 7 different concentration and combinations of auxin and cytokinin. Results indicated the germination rate were promoted by seeds mucilage, and were inhibited under cold condition. The best callus induction were observed from stem and leaf explants in the MS + B(IAA(0.1) + Kin(1) mg·L-10). The maximum leaf formation of leaf explants were produced in the MS + E(IAA(0.1) + BA(2) mg·L-10).
文摘Objective:To evaluate the effects of Zhumeria majdae essential oil(ZMEO)on morphine dependence and tolerance in mice.Methods:ZMEO(10,20,and 40 mg/kg)and clonidine(0.1 mg/kg)as the positive control were injected intraperitoneally(i.p.).The effect of ZMEO and clonidine on the dependence were evaluated by counting the number of jumps induced by naloxone(5 mg/kg)while the tolerance was evaluated by the tail-flick test.Results:ZMEO at the dose of 10 mg/kg during the development period led to a significant inhibition of morphine tolerance(P<0.01),while it led to reduced morphine dependence with the doses of 20 and 40 mg/kg.ZMEO at two dose levels of 20 and 40 mg/kg indicated significant antinociceptive activity(P<0.01),and significantly reduced the withdrawal signs(number of jumps)of mice(P<0.01).Conclusions:ZMEO had significant effects on morphine tolerance and dependence.The linalool rich essential oil of Zmajdae plays a major role in the reduction of tolerance and dependence induced by morphine.
