Objective:To investigate the effects of Sishen pill on the expression of type I interferon(IFN)and type III interferon and their receptors in colonic tissues of mice with acute ulcerative colitis(UC).Methods:Male C57B...Objective:To investigate the effects of Sishen pill on the expression of type I interferon(IFN)and type III interferon and their receptors in colonic tissues of mice with acute ulcerative colitis(UC).Methods:Male C57BL/6Cnc mice were randomly divided into control group,model group,sishenwan group and salazosulfapyridine group.The model was made with 0.2 mL 4%dextran sodium sulfate(DSS)for 5 days,and the control group was given 0.2mL normal saline by gavage.On the second day of modeling,sishen pill group was given 0.2mL 1.5 g·kg^(-1) sishen pill,and SASP group was given 0.2mL 0.25 g·kg^(-1) sulfasalazine,twice a day,for 7 days.During the administration period,the disease activity index(DAI)of mice was calculated every day.After administration,the histopathological changes of colon tissues of mice in each group were observed by hematoxylin eosin(HE)staining,and the histological scores were calculated.The expression of IFN-α,IFN-β,IFN-λ2 and IFN-λ3 mRNAs in colon tissues of mice in each group were detected by qRT-PCR.The expression levels of IFN-α,IFN-β,IFN-λ2 and IFN-λ3 in colon tissues of mice in each group were detected by ELISA.Western blot was used to detect the expression of interferon receptors IFNAR1,IFNAR2 and IFNLR1 in colon tissues of mice in each group.Results:Compared with the control group,the DAI of mice increased significantly(P<0.001)in the model group.The inflammatory cells in colonic tissues infiltrated heavily,lymph nodes enlarged,colonic mucosal structure destroyed,crypt structure lost,inflammation involved a wide range,and the histological score increased significantly(P<0.001).The levels of IFN-α,IFN-βand IFNλ2 mRNA were significantly decreased(P<0.05,P<0.05,P<0.01).The expression levels of IFN-α,IFN-β,IFN-λ2 and IFN-λ3 were significantly decreased(P<0.01,P<0.01,P<0.001,P<0.001).The levels of IFNAR1,IFNAR2 and IFNLR1 were significantly decreased(P<0.01,P<0.05,P<0.01).Compared with the model group,the DAI decreased significantly(P<0.001)in Sishen pill group,the infiltration of inflammatory cells in colon tissue were significantly reduced,the structural regeneration of colon mucosa was significantly recovered,the crypt structure was significantly recovered,the lymph nodes were significantly reduced,the range of inflammation involvement was reduced,and the histological score was significantly reduced(P<0.001).The levels of IFN-α,IFN-βand IFN-λ2 mRNA were significantly increased(P<0.01,P<0.001,P<0.001).The levels of IFN-α,IFN-β,IFN-λ2 and IFN-λ3 were significantly increased(P<0.01,P<0.001,P<0.01,P<0.001).The levels of IFNAR1,IFNAR2 and IFNLR1 were significantly increased(P<0.05,P<0.001,P<0.05).Conclusion:Sishen pill may alleviate the symptoms and signs of mice with acute ulcerative colitis by regulating the expression of type I and type III interferon and their receptors in colon tissues.展开更多
目的:建立同时测定党参固本丸中党参炔苷、苍术内酯Ⅲ、梓醇、毛蕊花糖苷、甲基麦冬烷酮A及甲基麦冬烷酮B 6种活性成分含量的测定方法,考察不同剂量60Co-γ射线辐照前后有效成分含量的变化。方法:采用Agilent Zorbax-C18色谱柱(250 mm&#...目的:建立同时测定党参固本丸中党参炔苷、苍术内酯Ⅲ、梓醇、毛蕊花糖苷、甲基麦冬烷酮A及甲基麦冬烷酮B 6种活性成分含量的测定方法,考察不同剂量60Co-γ射线辐照前后有效成分含量的变化。方法:采用Agilent Zorbax-C18色谱柱(250 mm×4.6 mm,5μm),以流动相为乙腈-0.4%磷酸溶液进行梯度洗脱,检测波长分别为210,330,220,296 nm,流速为1.0 ml·min-1,柱温为30℃,进样量为10μl。分别选择辐照剂量为2,5,5 k Gy对党参固本丸进行辐照,比较辐照前后党参固本丸中6种活性有效成分含量的变化,并经组间方差分析考察其显著情况。结果:党参炔苷、苍术内酯Ⅲ、梓醇、毛蕊花糖苷、甲基麦冬烷酮A及甲基麦冬烷酮B分别在0.462~18.464μg、1.006~40.250μg、1.794~71.754μg、0.981~39.236μg、0.477~19.072μg及0.450~18.016μg范围内有良好线性(r≥0.999 5)。经2,5,8 k Gy辐照后,党参固本丸所含的有效成分党参炔苷、苍术内酸Ⅲ、梓醇、毛蕊花糖苷、甲基麦冬烷酮A及甲基麦冬烷酮B均有变化,在超过5 k Gy后,苍术内酯Ⅲ辐照前后含量变化显著(P<0.05)。结论:所建立的党参固本丸活性成分测定方法回收率高,重复性好,简单实用,可作为党参固本丸的质控方法。在辐照剂量不超过5 k Gy时,各成分变化不显著(P>0.05),可为党参固本丸辐照灭菌手段提供参考。展开更多
基金National Natural Science Foundation of China(No.82160835)。
文摘Objective:To investigate the effects of Sishen pill on the expression of type I interferon(IFN)and type III interferon and their receptors in colonic tissues of mice with acute ulcerative colitis(UC).Methods:Male C57BL/6Cnc mice were randomly divided into control group,model group,sishenwan group and salazosulfapyridine group.The model was made with 0.2 mL 4%dextran sodium sulfate(DSS)for 5 days,and the control group was given 0.2mL normal saline by gavage.On the second day of modeling,sishen pill group was given 0.2mL 1.5 g·kg^(-1) sishen pill,and SASP group was given 0.2mL 0.25 g·kg^(-1) sulfasalazine,twice a day,for 7 days.During the administration period,the disease activity index(DAI)of mice was calculated every day.After administration,the histopathological changes of colon tissues of mice in each group were observed by hematoxylin eosin(HE)staining,and the histological scores were calculated.The expression of IFN-α,IFN-β,IFN-λ2 and IFN-λ3 mRNAs in colon tissues of mice in each group were detected by qRT-PCR.The expression levels of IFN-α,IFN-β,IFN-λ2 and IFN-λ3 in colon tissues of mice in each group were detected by ELISA.Western blot was used to detect the expression of interferon receptors IFNAR1,IFNAR2 and IFNLR1 in colon tissues of mice in each group.Results:Compared with the control group,the DAI of mice increased significantly(P<0.001)in the model group.The inflammatory cells in colonic tissues infiltrated heavily,lymph nodes enlarged,colonic mucosal structure destroyed,crypt structure lost,inflammation involved a wide range,and the histological score increased significantly(P<0.001).The levels of IFN-α,IFN-βand IFNλ2 mRNA were significantly decreased(P<0.05,P<0.05,P<0.01).The expression levels of IFN-α,IFN-β,IFN-λ2 and IFN-λ3 were significantly decreased(P<0.01,P<0.01,P<0.001,P<0.001).The levels of IFNAR1,IFNAR2 and IFNLR1 were significantly decreased(P<0.01,P<0.05,P<0.01).Compared with the model group,the DAI decreased significantly(P<0.001)in Sishen pill group,the infiltration of inflammatory cells in colon tissue were significantly reduced,the structural regeneration of colon mucosa was significantly recovered,the crypt structure was significantly recovered,the lymph nodes were significantly reduced,the range of inflammation involvement was reduced,and the histological score was significantly reduced(P<0.001).The levels of IFN-α,IFN-βand IFN-λ2 mRNA were significantly increased(P<0.01,P<0.001,P<0.001).The levels of IFN-α,IFN-β,IFN-λ2 and IFN-λ3 were significantly increased(P<0.01,P<0.001,P<0.01,P<0.001).The levels of IFNAR1,IFNAR2 and IFNLR1 were significantly increased(P<0.05,P<0.001,P<0.05).Conclusion:Sishen pill may alleviate the symptoms and signs of mice with acute ulcerative colitis by regulating the expression of type I and type III interferon and their receptors in colon tissues.
文摘目的:建立同时测定党参固本丸中党参炔苷、苍术内酯Ⅲ、梓醇、毛蕊花糖苷、甲基麦冬烷酮A及甲基麦冬烷酮B 6种活性成分含量的测定方法,考察不同剂量60Co-γ射线辐照前后有效成分含量的变化。方法:采用Agilent Zorbax-C18色谱柱(250 mm×4.6 mm,5μm),以流动相为乙腈-0.4%磷酸溶液进行梯度洗脱,检测波长分别为210,330,220,296 nm,流速为1.0 ml·min-1,柱温为30℃,进样量为10μl。分别选择辐照剂量为2,5,5 k Gy对党参固本丸进行辐照,比较辐照前后党参固本丸中6种活性有效成分含量的变化,并经组间方差分析考察其显著情况。结果:党参炔苷、苍术内酯Ⅲ、梓醇、毛蕊花糖苷、甲基麦冬烷酮A及甲基麦冬烷酮B分别在0.462~18.464μg、1.006~40.250μg、1.794~71.754μg、0.981~39.236μg、0.477~19.072μg及0.450~18.016μg范围内有良好线性(r≥0.999 5)。经2,5,8 k Gy辐照后,党参固本丸所含的有效成分党参炔苷、苍术内酸Ⅲ、梓醇、毛蕊花糖苷、甲基麦冬烷酮A及甲基麦冬烷酮B均有变化,在超过5 k Gy后,苍术内酯Ⅲ辐照前后含量变化显著(P<0.05)。结论:所建立的党参固本丸活性成分测定方法回收率高,重复性好,简单实用,可作为党参固本丸的质控方法。在辐照剂量不超过5 k Gy时,各成分变化不显著(P>0.05),可为党参固本丸辐照灭菌手段提供参考。
