In Vitro Anti-Bacterial and Anti-Fungal Activities of Extracts from Different Parts of 7 Zingiberaceae Plants

This study aimed to explore the anti-bacterial and anti-fungal activities of extracts from different parts of plants in the Zingiberaceae family.The inhibitory rate,minimum inhibitory concentration(MIC),and minimum bactericidal concentration(MBC)of leaf and stem,and root and rhizome extracts from Alpinia katsumadai Hayata,Alpinia oxyphylla Miq×Alpinia henryi K.Schumann,Alpinia oblongifolia Hayata,Alpinia nigra(Gaertn.)Burtt,Amomum villosum Lour,Alpinia zerumbet(Pers.)Burtt.et Smith and Alpinia oxyphylla Miq were determined using the fungus cake method and double dilution method.The seven Zingiberaceae plants exhibited characteristic antibacterial activities against pathogenic bacteria and fungi.At a 1.5 mg mL^(−1),A.zerumbet root and rhizome extracts exhibited strong inhibitory activity against S.aureus and E.coli,with 83.23%and 79.62%,respectively.In addition,A.zerumbet leaf and stem extracts had an inhibitory rate of 90.85%against P.aeruginosa.At the same concentration,the leaf and stem,root and rhizome extracts of A.katsumadai had the best anti-bacterial effect against F.oxysporum,with inhibition rates of 84.46%and 84.73%,respectively.Moreover,A.katsumadai and A.zerumbet leaf and stem extracts had the most significant antibacterial effect against S.aureus,with a MIC of 0.063 mg mL^(−1).Thus,both A.katsumadai and A.zerumbet extracts had significant antibacterial activity.In addition,by comparing the inhibitory effect of extracts from different parts,it was found that the inhibitory rate and average inhibitory rate of extracts from leaf and stem were higher than those from root and rhizome.The chemical constituents of A.katsumadai and A.zerumbet,determined by the high-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS),revealed that citric acid(CA),alpinetin,and pinocembrin(PNCB)were the functional constituents yielding the antibacterial activity.Overall,A.katsumadai and A.zerumbet have the potential to be developed as new plant fungicides and bactericides.

Main Pests and Diseases of Zingiberaceae and Their Control

The primary diseases affecting Zingiberaceae plants include ginger plague, spot blotch, anthracnose, leaf spot, leaf blight, and soft rot. Insect pests that pose a threat to these plants encompass root-knot nematode disease, drilling bugs, beet nightshade moths, mesquite, thrips, and aphids. This article aims to summarize the defining features of the principal pests and diseases as well as their control methods. The intention is to offer theoretical support for the preservation of ginger plants.

Pollen morphology of the genus Cornukaempferia (Zingiberaceae) in Thailand

Cornukaempferia is a recently described genus of Zingiberaceae which only occurs in Thailand as a rare genus with limited geographical distribution. Only three species have been described so far, including a recently described new species, C. larsenii. These three species are morphologically very similar and additional data on other biological aspects are useful for the elucidation of their relationship. Pollen morphology of all three species of genus Cornukaempferia has been studied by light microscopy and scanning electron microscopy. The pollen grains are monad, spherical, inaperturate. The exine sculpture is echinate with psilate between the spines for C. aurantiflora and C. longipetiolata, or echinate with regulate between the spines for C. larsenii. This observation helps support the taxonomic status of C. larsenii.

Chemical constituents and biological activities of essential oils of Amomum genus(Zingiberaceae)

Amomum Roxb.includes the aromatic and medicinal plants native to tropical and subtropical Asia belonging to the family Zingiberaceae.Members of Amomum genus have been used for a long time in traditional medicine for the treatment of throat trouble,congestion of lungs,inflammation of eyelids,and digestive disorders,etc.Amomum essential oils have been studied for their chemical profiles in which limonene,allo-aromadendrene,1,8-cineole,camphor,farnesyl acetate,α-pinene,β-pinene,caryophyllene,camphene,D-camphor,santolina triene,methyl chavicol,bornyl acetate,β-elemene,δ-3-carene,etc.were the major compounds.Furthermore,the oils extracted from Amomum plants have been reported to possess antimicrobial,antioxidant,insecticidal,larvicidal,cytotoxic,anti-scabies,and anti-inflammatory activities.This review focuses on the chemical constituents and biological activities of the essential oils isolated from the different plant parts of Amomum plants.The objective of the present review is to highlight therapeutic potentials and provide evidence for future medicinal applications of these species of genus Amomum.

Reproductive Ecology of Rhynchanthus beesianus W. W. Smith （Zingiberaceae）in South Yunnan, China： A Ginger with Bird Pollination Syndrome

Rhynchanthus beesianus W. W. Smith （Zlnglberaceae） Is an eplphytlc tropical ginger with a very conspicuous floral display, but almost no fruit set under field conditions. The reproductive ecology encompassing phenology, floral biology, and pollination and breeding systems was Investigated In an evergreen broadleaved forest In Yunnan Province, Southwest China. The flowers possess a typical bird pollination syndrome, but no effective pollinators were observed during 138 h of observation. Female Black-breasted Sunbird （Aethopyga saturata） and bumblebees visited R. beesianus regularly, but they all played roles as nectar robbers. No fruit was found In the bagging treatment, and fruit set following manual self-pollination （（57.55 ± 4.08）%） was comparable with cross-pollination （（64.32 ± 4.42）%）, suggesting that R. beeslanus is self-compatible but spontaneous self-pollination In this species does not occur. Seed set of open-pollination （（26.42 ± 3.11）%） was significantly lower than manual self-pollination （（73.41± 4.16）%） and cross-pollination （（75.56 ± 4.52）%）, confirming that R. beeslanus was dependent on animals for fertilization and suffered a serious pollinator-limitation.

Prospects for discriminating Zingiberaceae species in India using DNA barcodes

We evaluated nine plastid （matK, rbcL, rpoCl, rpoB, rp136-rpsS, ndhJ, trnL-F, tmrnH-psbA, accD） and two nuclear （ITS and ITS2） barcode loci in family Zingiberaceae by analyzing 60 accessions of 20 species belonging to seven genera from India. Bidirectional sequences were recovered for every plastid locus by direct sequencing of polymerase chain reaction （PCR） amplicons in all the accessions tested. However, only 35 （58%） and 4o accessions （66~） yielded ITS and ITS2 sequences, respectively, by direct sequencing. In different bioinformatics analyses, matK and rbcL consistently resolved 15 species （75%） into monophyletic groups and five species into two para- phyletic groups. The 173 ITS sequences, including 138 cloned sequences from 23 accessions, discriminated only 12 species （6o%）, and the remaining species were entered into three paraphyletic groups. Phylogenetic and genealogic analyses of plastid and ITS sequences imply the possible occurrence ofnatural hybridizations in the evolutionary past in giving rise to species paraphyly and intragenomic ITS heterogeneity in the species tested. The results support using matK and rbcL loci for barcoding Zingiberaceae members and highlight the poor utility of iTS and the highly regarded ITS2 in barcoding this family, and also caution against proposing ITS loci for barcoding taxa based on limited sampling.

Complete chloroplast genome sequence of Amomum villosum and comparative analysis with other Zingiberaceae plants

Objective:Amomum villosum(AV)is an herb whose dried fruit has been extensively used in modern medicine to treat digestive system diseases such as dysentery,vomiting and abdominal pain.This paper aims to supplement chloroplast(cp)genomic resources and to be used in phylogenetic studies and identification of AV related plants.Methods:High-throughput sequencing technology was used to determine the complete sequence of the AV cp genome,and the sequence was then compared with three related species.Results:The genome size of AV we obtained was 163,968 bp with an obvious tetrad structure.The AV cp genome was observed to contain 125 unique genes and 81 simple sequence repeat(SSRs)had been determined and the majority of which were adenine–thymine(AT)-rich.Comparative analysis of genome sequence of four ginger plants showed that the atpF,clpP and rpl32 genes are potential markers for identifying Amomum species.Phylogenetic analysis suggested that AV was closely related to A.kravanh and A.compactum.Conclusion:These results have brought useful genetic resources for further identification researches,DNA barcoding,resolving taxonomy and understanding the evolutionary mode of Zingiberaceae cp genome.

Spirematospermum wetzleri (Heer) Chandler (Zingiberaceae) from the Miocene of Weichang, Hebei Province, North China and the phytogeographic history of the genus

Spirematospermum is a well-known extinct zingiberalean taxon, characterized by trilocular capsules containing many distinctive, spirally striate and arillate seeds. It is frequently found and studied in European Neogene carpological floras, but is scarcely represented in East Asia floras. In this work we recognize a new fossil record of Spirematospermum wetzleri（Heer） Chandler based on the capsules and seeds from the Miocene of Weichang, Hebei Province, North China. These fossils represent the first record of the species in the Miocene of China. Fossil data indicate that Spirematospermum probably originated in the Late Cretaceous of North America or Central Europe.The genus still existed in the Paleocene of North America, but became extinct after that time. However, the genus successively survived in Europe from the Eocene to Pliocene, and flourished luxuriantly during the Oligocene to Miocene. As there was Turgai Strait between Europe and Asia during the Eocene, the genus did not spread to Central Asia and West Siberia until the strait closed in the late Eocene/early Oligocene, and further expanded eastwardly to eastern Siberia, Russia, northern China and central Japan during the Miocene, but became extinct in Asia after the Miocene. The genus contracted its distribution to Europe in the Pliocene, and afterwards it became extinct in the world.

温郁金地上部分的化学成分(英文)

目的:研究中药莪术来源植物之一温郁金地上部分的化学成分。方法:利用反复硅胶色谱柱进行分离纯化,通过理化性质和波谱数据分析鉴定化合物结构。结果:分离并鉴定出6种化合物:codonolactone(1),vole-neol(2),octacosanoic ac id(3),β-谷甾醇(4),m angdesisterol(5)和胡萝卜苷(6)。结论:两个倍半萜化合物1,2和一个甾醇5为首次从该植物中分得。

广东姜黄属一新变种——南岭莪术

描述了广东姜科（Zingiberaceae）姜黄属（Curcuma）的一个新变种——南岭莪术（C.kwangsiensis var. nanlingensis N. Liu et X.Y.Ma）.该新变种与原变种的区别在于植株矮小,株高仅0.4-0.6 m,主根茎直径仅2-3 cm、长约3-4 cm,孕苞片白绿色.另检测表明该变种的体细胞染色体为2n=84.

Oxyphyllones A and B,novel sesquiterpenes with an unusual 4,5-secoeudesmane skeleton from Alpinia oxyphylla

Two novel 4,5-secoeudesmane sesquiterpenoids, oxyphyllones A （1） and B （2） were isolated from the fruits of Alpinia oxyphylla. Their structures were established by spectroscopic methods including 1D and 21） NMR spectra. These two compounds are the first example of naturally occurring sesquiterpenoids with a 4,5-secoeudesmane skeleton in the family of Zingiberaceae and oxyphyllone A （1） is the first 4,5-secoeudesmane type of 13-norsesquiterpenoid. Compounds 1 and 2 exhibited no cytotoxicities against three cancer cell lines at 10 μg/mL. ？ 2009 Ning Hua Tan. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.

Evaluation of rapid molecular diagnostics for differentiating medicinal Kaempferia species from its adulterants

Accurate detection of unique herbs is crucial for herbal medicine preparation. Zingiberaceae species, which are important in Ayurvedic medicine of India, are often misidentified in Northeast (NE) Indian herbal markets. Kaempferia galanga (Zingiberaceae) is one of the major components of popular Ayurvedic drugs used for rheumatic diseases (i.e.,“Gandha Thailam” and “Rasnairandadi Kashayam”), contusions, fractures, and sprains. In NE India, herbal healers often misidentify plants from the Marantaceae family (e.g., Calathea bachemiana and Maranta leuconeura) as Kaempferia, which leads to adulteration of the medicinal herb. This misidentification of herbs occurs in NE India because Zingiberaceae plant barcoding information is inadequate. As a consequence, herbal medicine is not only therapeutically less effective but may also cause adverse reactions that range from mild to life-threatening. In this study, we used eight barcoding loci to develop “fingerprints” for four Kaempferia species and two species frequently mistaken for Kaempferia. The PCR and sequencing success of the loci matK, rbcL and trnH-psbA were found to be 100%;the combination of matK, rbcL, and trnH-psbA proved to be the ideal locus for discriminating the Kaempferia species from their adulterants because the combined loci showed greater variability than individual loci. This reliable tool was therefore developed in the current study for accurate identification of Kaempferia plants which can effectively resolve identification issues for herbal healers.

New Sesquiterpenoids from Hedychium yunnanense gagnep

Two new sesquiterpenoid trialcohol isomers named 1 beta, 4 alpha 11 alpha -trihydroxyeudesmane (1) and Yunnanensehedychetriol (2), were isolated from the fresh rhizomes of Hedychium Yunnanense gagnep. Their structures were elucidated by spectroscopic methods.

Antioxidant and antigenotoxic properties of Alpinia galanga,Curcuma amada,and Curcuma caesia

Objective:To compare the antioxidant and anti-genotoxic properties of Alpinia(A.)galanga,Curcuma(C.)amada,and C.caesia.Methods:Cytotoxicity of ethanolic extracts of A.galanga,C.amada,and C.caesia at selected doses was evaluated by trypan blue,MTT,and flow cytometry-based assays.Genotoxicity and anti-genotoxicity(against methyl methanesulfonate,35μM and H2O2,250μM)of these plants were studied by comet assay in human lymphocytes in vitro.Furthermore,DPPH,ABTS,FRAP,lipid peroxidation,and hydroxyl radical scavenging assays were performed to study the antioxidant potentials of the plants.Finally,anti-genotoxic potential of C.amada was validated in Swiss albino mice using comet assay.Phytochemical composition of C.amada was determined by GC/MS and HPLC.Results:The selected doses(2.5,5,and 10μg/mL)of A.galanga,C.amada,and C.caesia were non-toxic by cytotoxicity tests.All three ethanolic extracts of plant rhizomes demonstrated antioxidant and anti-genotoxic properties against methyl methanesulfonate-and H2O2-induced oxidative stress in human peripheral blood lymphocytes in vitro.Multivariate analysis revealed that various antioxidant properties of these extracts in DPPH,ABTS,and FRAP assays were strongly correlated with their total phenolic constituents.C.amada extract conferred protection against cyclophosphamide-induced DNA damage in the bone marrow cells of mice and DNA damage was significantly inhibited by 2.5 mg/kg C.amada extract.Conclusions:C.amada is rich in potentially bioactive molecules and exhibits potent antioxidant activities.Its anti-genotoxicity against cyclophosphamide-induced oxidative stress is also confirmed in this study.

ISSR Fingerprinting to Ascertain the Genetic Relationship of Curcuma sp. of Tripura

Molecular fingerprints of four different species of Curcuma, viz., C. amada, C. caesia, C. longa and C. zedoaria, found in Tripura were developed using Inter Simple Sequence Repeats. Twenty ISSR primers generated 116 loci amplified in the range of 200 - 5000 bp with an average of 5.8 alleles and 1.6 effective alleles per locus. The percentage of polymorphic band was found to be 86.29 with an average of 5.15 per primer. Based on UPGMA algorithm these four species are placed in two different clusters that validate the classification based on external and internal morphological characters. The polymorphic ISSR markers generated from this study will be useful for understanding the genetic relationship of different species of the genus Curcuma.

Quantification of Thiamethoxam in Rhizomes and Leaves of the Hedychium coronarium and Water and Soil by High-Pressure Liquid Chromatography

The linearity, stability, accuracy and inter-day precisions of the assay method were evaluated in water, soil and rhizomes and leaves of Hedychium coronarium of the Zingiberaceae family. Mato Grosso do Sul is a predominantly agricultural state in Brazil and has many rivers and permanent and seasonal lakes, in which the Hedychium coronarium, an aquatic plant, is found the swampy environments. The prepared samples were analyzed quantitatively by High Performance Liquid Chromatography with PDA and UV detection for the presence of thiamethoxam. The thiamethoxam was recovered from these samples at rates ranging from 81.16% - 99.93%. The coefficient of variation in the quantitative analysis of the thiamethoxam was under 5%. The linearity of the method was determined by linear regression. The analysis of the samples spiked with known amounts of analyte demonstrated that the response was proportional to the concentrations of the samples with determination coefficients of r2 = 0.9992 (water and soil) and r2 = 0.9990 (leaves and rhizomes) for the linear range of the analytical calibration curves of the samples. The detection limit was 0.36 μg.L-1 and quantification limit was 1.2 μg.L-1 for thiamethoxam. The method was considered sensitive for quantification of the thiamethoxam in water, soil and rhizomes and leaves of Hedychium coronarium.

Phytochemistry and pharmacology of ornamental gingers,Hedychium coronarium and Alpinia purpurata:a review

In this review, the phytochemistry and pharmacology of two ornamental gingers, Hedychium coronarium （butterfly ginger） and Alpinia purpurata （red ginger）, are updated, and their botany and uses are described. Flowers of H. coronarium are large, showy, white, yellow or white with a yellow centre and highly fragrant. Inflorescences of A. purpurata are erect spikes with attractive red or pink bracts. Phytochemical investigations on the rhizomes of H. coronarium generated research interest globally. This resulted in the isolation of 53 labdane-type diterpenes, with little work done on the leaves and flowers. Pharmacological properties of H. coronarium included antioxidant, antibacterial, antifungal, cytotoxic, chemopreventive, anti-allergic, larvicidal, anthelminthic, analgesic, anti-inflammatory, anti-urolithiatic, anti-angiogenic, neuro- pharmacological, fibrinogenolytic, coagulant and hepatoprotective activities. On the contrary, little is known on the phytochemistry of A. purpurata with pharmacological properties of antioxidant, antibacterial, larvicidal, cytotoxic and vasodilator activities reported in the leaves and rhizomes. There is much disparity in terms of research effort within and between these two ornamental gingers.

Bisabocurcumin,a new skeleton curcuminoid from the rhizomes of Curcuma longa L.

A new skeleton bisabolane-type sesquiterpene curcuminoid,bisabocurcumin（1）,along with 5 known compounds,curcumin（2）, demethoxycurcumin（3）,bidemethoxycurcumin（4）,（1E,4E）-1,5-bis（4-hydroxy-3-methoxyphenyl）-penta-1,4-dien-3-one（5）,and （1E,4E）-1-（4-hydroxy-3-methoxyphenyl）-5-（4-hydroxy phenyl-）-penta-1,4-dien-3-one（6）were isolated from the rhizomes of Curcuma longa L.Their structures were determined on the basis of spectroscopic analysis.Bisabocurcumin（1） is firstly obtained from nature with a new skeleton combined by a bisabolane-type sesquiterpene and a 1,7-diphenylheptanoid through a C-C bond.

Analgesic and Anti-inflammatory Effects of Ginger Oil

Objective Ginger(Zingiber officinale) is widely used as a spice in cooking and as a medicinal herb in traditional herbal medicine.The present study was to investigate the analgesic and anti-inflammatory activities of ginger oil in experimental animal models.Methods The analgesic effect of the oils was evaluated by the 'acetic acid' and 'hot-plate' test models of pain in mice.The anti-inflammatory effect of the oil was investigated in rats,using rat paw edema induced by carrageenan,adjuvant arthritis,and vascular permeability induced by bradykinin,arachidonic acid,and histamine.Indomethacin(1 mg/kg),Aspirin(0.5 g/kg) and Dexamethasone(2.5 mg/kg) were used respectively as reference drugs for comparison.Results The ginger oil(0.25-1.0 g/kg) produced significant analgesic effect against chemically-and thermally-induced nociceptive pain stimuli in mice(P < 0.05,0.01).And the ginger oil(0.25-1.0 g/kg) also significantly inhibited carrageenan-induced paw edema,adjuvant arthritis,and inflammatory mediators-induced vascular permeability in rats(P < 0.05,0.001).Conclusion These findings confirm that the ginger oil can be used to treat pain and chronic inflammation such as rheumatic arthritis.

The genomic and bulked segregant analysis of Curcuma alismatifolia revealed its diverse bract pigmentation

Compared with most flowers where the showy part comprises specialized leaves(petals)directly subtending the reproductive structures,most Zingiberaceae species produce showy“flowers”through modifications of leaves(bracts)subtending the true flowers throughout an inflorescence.Curcuma alismatifolia,belonging to the Zingiberaceae family,a plant species originating from Southeast Asia,has become increasingly popular in the flower market worldwide because of its varied and esthetically pleasing bracts produced in different cultivars.Here,we present the chromosome-scale genome assembly of C.alismatifolia“Chiang Mai Pink”and explore the underlying mechanisms of bract pigmentation.Comparative genomic analysis revealed C.alismatifolia contains a residual signal of whole-genome duplication.Duplicated genes,including pigment-related genes,exhibit functional and structural differentiation resulting in diverse bract colors among C.alismatifolia cultivars.In addition,we identified the key genes that produce different colored bracts in C.alismatifolia,such as F3′5'H,DFR,ANS and several transcription factors for anthocyanin synthesis,as well as chlH and CAO in the chlorophyll synthesis pathway by conducting transcriptomic analysis,bulked segregant analysis using both DNA and RNA data,and population genomic analysis.This work provides data for understanding the mechanism of bract pigmentation and will accelerate breeding in developing novel cultivars with richly colored bracts in C.alismatifolia and related species.It is also important to understand the variation in the evolution of the Zingiberaceae family.