(Short communication) Degradation of corrosion resistance of Zircaloy-4 in LiOH aqueous solution

To investigate the degradation of corrosion resistance of Zircaloy-4 in LiOHaqueous solution, SIMS (secondary ion mass spectrometry) analysis was performed to examine theprofiles of Li^+, K^+, and OH^- in oxide layers formed in the same concentration (0.1 mol/L) LiOHand KOH solutions. Even though the oxide layers have an equal thickness, the pene-tration depth ofK^+ is shallower than that of Li^+, and the penetration depth of OH^- corroded in KOH solution isalso shal-ower than that corroded in LiOH solution. It shows that the diffusion of OH^- into oxidelayer is accompanied by the corresponding cation. The difference of degradation effect of LiOH andKOH solutions on the corrosion resistance of Zircaloy-4 was discussed.

Microstructure evolution of zircaloy-4 during Ne ion irradiation and annealing: An in situ TEM investigation

The microstructural evolution of zircaloy-4 was studied, including the amorphization and recrystallization of Zr（Fe, Cr）2 precipitates, and the density of dislocations under in situ Ne ion irradiation and post annealing. The results show that irradiation at a relatively high temperature and dose induces the formation of nanocrystals in pre-amorphized Zr（Fe, Cr）2 precipitates. The recrystallized nanocrystals also have the structure of hcp-Zr（Fe, Cr）2. The formation of the nanocrystals is thought to be the consequence of competition between atomistic disordering and the recrystallization of precipitates under ion irradiation. The free energy of the nanocrystal is lower than that of the amorphous state, which is another reason for the recrystallization of the precipitates. With increased annealing temperature, the density of the nanocrystals is increased. The dislocation density sharply decreases with the increase in the annealing temperature, and its size increases.

Fretting wear behavior of nitrogen implanted Zircaloy-4 alloy

Zircaloy 4 was implanted with nitrogen at 120 keV with various ion doses between 1×10 13 and 1×10 14 ions/m 2. Fretting wear tests were performed at various cycles and loads under water immersion condition by the fretting simulator, The implanted surfaces were analyzed by Auger electron spectroscopy (AES) and transmission electron microscope (TEM). Micro hardness tester measured surface hardness. It is shown that nitrogen implantation produced Zirconium nitride oxide and high density dislocations in implanted layer, surface hardness was enhanced from HK280 for unimplanted specimen to HK1 800 for a total ion dose of 1×10 14 ions/m 2. The nitrogen ion implantation treatment provided significant improvements in the resistance of fretting damage.

CYCLIC DEFORMATION BEHAVIOR OF ZIRCALOY-4 AT DIFFERENT TEMPERATURES

Fatigue lifetime curves of Zircaloy-4 at different temperatures were measured, and the fatigued dislocation configurations were investigated using transmission electron microscopy. Ii was found that prismatic slip is the primary deformation mode. for Zircaloy-4fatigued at RT prismatic plus Pyramidal slip at 400℃,and prismatic plus (0001) basic slip at 600℃. The corresponding dislocation configurations are parallel dislocation lines al RT, elongated cells at 400℃ and two sets of mutually Perpendicular dislocation bands at 600℃.respectively. The relationship among. fatigue deformation mode, dislocation configuration and lifetime is discussed.

Irradiation damage simulation of Zircaloy-4 using argon ions bombardment

To simulate irradiation damage, argon ion was implanted in the Zircaloy-4 with the fluence ranging from 1 × 10^16 to 1 × 10^17 cm^-2, using accelerating implanter at an extraction voltage of 190 kV and liquid nitrogen temperature. Then the influence of argon ion implantation on the aqueous corrosion behavior of Zircaloy-4 was studied. The valence states of elements in the surface layer of the samples were analyzed using X-ray photoelectron spectroscopy （XPS）. Transmission electron microscopy （TEM） was used to examine the microstructure of the argon-implanted samples. The potentiodynamic polarization technique was employed to evaluate the aqueous corrosion resistance of implanted Zircaloy-4 in 1 mol/L HzSO4 solution. It is found that there appear bubbles on the surface of the samples when the argon fluence is 1 × 10^16 cm^-2. The microstructure of argon-implanted samples changes from amorphous to partial amorphous, then to polycrystalline, and again to amorphous. The corrosion resistance of implanted samples linearly declines with the increase of fluence approximately, which is attributed to the linear increase of the irradiation damage.

Impact of high dose Kr^+ ion irradiation on the corrosion behavior and microstructure of Zircaloy-4

In order to investigate the ion irradiation effect on the corrosion behavior and microstructure of Zircaloy-4, the Zircaloy-4 film were prepared by electron beam deposition on the Zircaloy-4 specimen surface and irradiated by Kr ions using an accelerator at an energy of 300 keV with the dose from 1×1015 to 3×1016ions/cm2. The post-irradiation corrosion tests were conducted to rank the corrosion resistance of the resulting specimens by potentiodynamic polarization curve measurements in a 0.5 mol/L H2SO4 water so- lution at room temperature. Transmission electron microscopy (TEM) was employed to examine the microstructural change in the surface. The potentiodynamic tests show that with the irradiation dose increasing, the passive current density, closely related to the surface corrosion resistance, decreases firstly and increases subsequently. The mechanism of the corrosion behavior transformation is due to the amorphous phase formation firstly and the amorphous phase destruction and the polycrystalline structure formation in the irradiated surface subsequently.

Study of Oxidation Kinetics in Air of Zircaloy-4 by <i>in Situ</i>X-Ray Diffraction

The zircaloy-4 is an alloy of zirconium, which has a very weak thermal neutron absorption, satisfactory mechanical properties and good corrosion resistance at high temperature. For these reasons, zircaloy-4 is used as a material of cladding fuel rod of nuclear reactors. In this environment, it is submitted to different severe conditions of temperature and pressure. The objective of this work is to study the oxidation kinetics of zircaloy-4 in air by the X-ray diffraction technique. The experiments were realized in a “HTK1200” furnace installed as a sample holder in the diffractometer at different temperatures;25°C, 350°C, 500°C, 830°C and 1000°C. The results show that the monoclinic and the tetragonal phases are formed at 350°C temperature. The volume fraction of these phases increased with the temperature until 1000°C where the α phase disappears completely. For simulating the case of loss-of-coolant-accident (LOCA), we have done x-ray diffraction of Zry-4 samples water quenched at 1050°C with different ageing times at this temperature. At 10 seconds and more, there is an important evolution of monoclinic and tetragonal zirconias, which leads to the degradation of zircaloy-4 properties.

Hydrogen Pick up in Zircaloy-4: Effects in the Dimensional Stability of Structural Components under Nuclear Reactor Operating Conditions

In the present work, the expansion coefficient due to hydrogen incorporation was measured for the axial direction of a Zircaloy-4 cooling channel, similar to that installed in the Atucha I PHWR, Argentina, trying to simulate the nuclear power reactor operating conditions. As a first step, the solubility curve of hydrogen in Zircloy-4 was determined by two techniques: differential scanning calorimetry and differential dilatometry. The comparison with classical literature curves showed a good agreement with them, although the calorimetric technique proved to be more accurate for these determinations. Dilatometry was able to detect the end of hydride dissolution from concentrations around 60 wppm-H up to 650 wppm-H, where the eutectoid reaction: α + δ→α + β takes place (at 550oC). We assume that this ability is a good indicator of the aptitude of the technique to measure dimensional changes in the given hydrogen concentration range. Then, the expansion of Zircaloy-4 homogeneously hydrided samples was measured at 300oC, the typical operating temperature of a nuclear power reactor, obtaining a relative expansion of 2.21 * 10-4% per wppm-H. Considering the relative expansion observed for Zircaloy-4 at room temperature due to hydriding, starting from a hydrogen free sample, the total relative expansion rate is calculated to be 5.21 * 10-4% per wppm-H.

The Cracking Induced by Oxidation-Hydriding in Welding Joints of Zircaloy-4 Plates

The welding joints of Zircaloy 4 plates obtained by diffusion welding at 800°C under pressure in vacuum were cracked during autoclave tests at 400°C superheated steam after exposure longer than 150 days. The section of specimens was examined by optical microscopy and the composition at the tips of cracking was analyzed by electron microprobe. The result shows that the combination of oxidation and hydriding induced cracking is responsible for this failure of the welding joints.

血清PCT、CRP及IL-4水平预测小儿支原体肺炎病情严重程度的价值

目的:探讨血清降钙素原(PCT)、C反应蛋白(CRP)及白细胞介素-4(IL-4)水平预测支原体肺炎患儿病情严重程度的价值。方法:选取2019年1月—2023年1月淮安市第二人民医院儿科收治的102例支原体肺炎患儿作为研究对象,根据病情将患儿分为轻症组59例和重症组43例。比较两组临床资料及基质细胞衍生因子(CXCL12)、γ干扰素(IFN-γ)、硫化氢(H_(2)S)、超氧化物歧化酶(SOD)、基质金属蛋白酶-9(MMP-9)、PCT、CRP及IL-4水平,多因素分析采取非条件logistic逐步回归分析,采用ROC曲线分析PCT、CRP及IL-4水平对重症支原体肺炎的预测价值。结果:两组性别、年龄、病程及CXCL12、IFN-γ、H_(2)S、SOD、MMP-9水平比较,差异无统计学意义(P>0.05);重症组PCT、CRP、IL-4水平显著高于轻症组,差异有统计学意义(P<0.05)。logistic逐步回归分析结果显示,PCT、CRP及IL-4为重症支原体肺炎独立危险因素(P<0.05)。ROC分析显示,PCT、CRP及IL-4预测重症支原体肺炎的曲线下面积分别为0.896、0.851、0.787。结论:血清PCT、CRP及IL-4水平均参与支气管肺炎患儿的病情进展,且可作为重症支气管肺炎的诊断指标。

Ⅰ群4型禽腺病毒Y17215-1株体外增殖规律及免疫原性研究

为探讨禽腺病毒4型分离株Y17215-1的体外增殖特性和免疫原性,本研究以LMH细胞为模型,摸索Y17215-1株最佳接种剂量和收获时间;通过活毒滴鼻接种和灭活疫苗肌肉注射的方式免疫SPF鸡,测定其免疫原性。结果显示:Y17215-1株0.1 MOI接种LMH细胞,培养48~72 h病毒滴度达到峰值108.625 TCID_(50)/mL。该病毒经肌肉注射对42日龄SPF鸡具有较强致病性,LD_(50)为103.000 TCID_(50)/0.2 mL。以10^(7.676) TCID_(50)的Y17215-1株滴鼻免疫21日龄SPF鸡,免疫后7 d 100%试验鸡产生中和抗体,14 d达到高峰。以Y17215-1株制备灭活疫苗肌肉注射免疫21日龄SPF鸡(10^(7.676) TCID_(50)/羽),免疫后7 d 70%试验鸡产生中和抗体,21 d达到高峰。在免疫后21 d用1000LD_(50)的Y17215-1株进行攻毒,活毒和灭活疫苗的保护率均为100%。以上结果表明:Y17215-1株具有很好的体外增殖特性和免疫原性,可以作为候选疫苗毒株。

胶质瘤组织中TRIP4和DDIT4水平表达及其与临床病理特征和预后的关系

目的研究胶质瘤组织中甲状腺激素受体结合蛋白4(thyroid hormone receptor interacting protein 4,TRIP4)和DNA损伤诱导转录因子4(DNA damage inducing transcription factor 4,DDIT4)水平表达及其与临床病理特征和预后的关系。方法选取2018年2月~2019年2月河北医科大学第一医院收治的94例胶质瘤患者为研究对象。应用免疫组织化学法检测组织中TRIP4和DDIT4蛋白表达。比较不同临床病理特征脑胶质瘤组织中TRIP4和DDIT4蛋白表达。采用Kaplan-Meier生存曲线分析不同TRIP4和DDIT4蛋白表达胶质瘤患者生存预后的差异。单因素和多因素COX回归分析影响胶质瘤患者生存预后的因素。结果胶质瘤组织中TRIP4(68.09%)和DDIT4(65.96%)蛋白阳性率高于瘤旁组织(13.83%,10.64%),差异具有统计学意义(χ^(2)=57.212,60.866,均P<0.05)。胶质瘤组织中TRIP4与DDIT4蛋白表达呈显著正相关性(r=0.722,P<0.05)。WHO分级Ⅲ级、肿瘤直径≥3cm胶质瘤组织中TRIP4和DDIT4蛋白阳性率均高于WHO分级Ⅰ~Ⅱ级、肿瘤直径<3cm胶质瘤组织,差异具有统计学意义(χ^(2)=6.393~14.754,均P<0.05)。TRIP4阳性表达组和阴性表达组三年总体生存率分别为37.50%(24/64),66.67%(20/30),TRIP4阳性表达组三年累积生存率低于阴性表达组,差异具有统计学意义(Log-rankχ^(2)=5.949,P=0.015)。DDIT4阳性表达组和阴性表达组三年总体生存率分别为37.10%(23/62),70.00%(21/30),DDIT4阳性表达组三年累积生存率低于阴性表达组,差异具有统计学意义(Log-rankχ^(2)=7.642,P=0.006)。肿瘤直径≥3cm(HR=1.614,P=0.000),WHO分级Ⅲ级(HR=1.790,P=0.000),TRIP4阳性表达(HR=1.665,P=0.000),DDIT4阳性表达(HR=1.476,P=0.000)是影响胶质瘤患者生存预后的独立危险因素。结论胶质瘤组织中TRIP4和DDIT4蛋白表达升高,两者与肿瘤直径及WHO分级相关,是潜在的评估胶质瘤预后的肿瘤标志物。

香蕉枯萎病菌内源报告基因Foc4carS的鉴定及其应用

香蕉枯萎病是由尖孢镰刀菌古巴转化型(Fusarium oxysporum f. sp. cubense, Foc)引起的香蕉毁灭性土传病害,其中4号生理小种(Foc4)能感染几乎所有的香蕉品系,危害最严重。carS基因通过调控下游car结构基因参与调控镰刀菌类胡萝卜素的生物合成,本研究克隆鉴定了Foc4carS基因(FOIG_05085),Foc4carS蛋白具有典型的RING-finger蛋白结构域。利用分割标记法(Split-marker PCR)获得Foc4carS基因的融合片段,同时构建含有Foc4carS基因sgRNA591序列的pUC-fFuCas9-HTBNLS-hph-Foc4carS基因编辑载体,通过PEG介导的原生质体转化获得该基因的敲除突变体、回补突变体以及基因编辑敲除体,并对敲除和回补突变体的生物学特性和致病力进行分析。结果显示:ΔFoc4carS突变体的菌落直径、产孢量和致病力等生物学表型与野生菌株Foc4无显著差异,而ΔFoc4carS突变体菌落颜色呈深橙色,Foc4carS基因的缺失影响了次生代谢产物类胡萝卜素的生物合成;基因编辑的ΔFoc4carS(HDR)突变体不论是再生筛选板还是继代后的PDA平板,其菌落均出现典型的深橙色,表明Foc4carS可作为内源报告基因,在香蕉枯萎菌Foc4中进行基因质粒型CRISPR/Cas9编辑可行。

全雌型工业大麻杂交新品种云麻杂4号的选育与应用前景

云麻杂4号是云南省农业科学院经济作物研究所以雌雄异株品种云麻8号近交系为母本,雌雄同株2017M5近交系为父本,利用杂种优势组配而成的工业大麻全雌型晚熟杂交种。2020—2021年多点区域试验中花叶平均产量3012.45 kg/hm^(2),比对照云麻8号增产34.82%。该品种雌株比例大于95%,全生育期约167 d,THC平均含量为0.07%,CBD平均含量3.06%,籽粒含油量31.8%,蛋白质含量23.0%。适合云南省大部分地区种植,最适宜海拔范围为1500~2300 m。2023年通过云南省品种鉴定和登记。

瞬时NH_(4)^(+)-N冲击对SBR系统污染物去除的影响

探究了瞬时NH_(4)^(+)-N冲击对SBR系统污染物去除效果的影响,在反应运行的第19、33、45天,通过提高单周期瞬时NH_(4)^(+)-N质量浓度至41.05、60.02、80.15 mg/L,对SBR系统进行冲击。结果表明,进水NH4+-N质量浓度从41.05 mg/L增加到80.15 mg/L时,COD、TP、NH_(4)^(+)-N去除效果没有明显变化,TN出水质量浓度从10.25 mg/L增加到21.49 mg/L,TN的出水变高主要受碳源不足的影响。三次瞬时NH4+-N冲击负荷下,NH4+-N降解速率基本不变。三次瞬时NH_(4)^(+)-N冲击负荷下,SBR系统中EPS总量对比常负荷运行时,均有所提高,且PN的含量在增加,PS的含量先升高后降低。而PN和PS含量的变化,在一定程度上会对活性污泥的絮凝造成影响,导致活性污泥粒径逐渐减少。系统在受到瞬时NH_(4)^(+)-N冲击后,TN出水会有所提高,但是经过一个周期的运行便可以恢复。

玉米纹枯病菌中新减毒病毒RsHV4的特征研究

寻找立枯丝核菌(Rhizoctonia solani)中新的弱毒病毒,为玉米纹枯病提供新的生物防治资源。采用dsRNA技术从玉米纹枯病菌菌株ZYFG11中发现一种新的病毒RsHV4(Rhizoctonia solani hypovirus4),通过基因组克隆、系统发育分析、菌株生长特性测定及致病性测定等试验,对新病毒RsHV4的分类地位、减毒活性等生物学特征进行测定。结果表明,RsHV4的基因组全长为18702 nt,编码一个含5540个氨基酸的多聚蛋白(Polyprotein);系统发育分析显示,RsHV4与减毒病毒科(Hypoviridae)θ-减毒病毒属(Thetahypovirus)病毒聚为一支,其编码的蛋白质与该属的Sclerotium rolfsii hypovirus 1编码的蛋白质同源性最高,为59.46%;立枯丝核菌正常菌株JSJH6与含毒菌株ZYFG11的菌丝生长速度分别为0.50cm/d和0.41 cm/d,菌核大量形成的时间分别为培养5 d和7 d,培养10 d时50颗最大菌核的总质量分别为0.27 g和0.11 g,显示含毒菌株ZYFG11的菌丝生长速度减慢、菌核形成时间延迟、菌核体积和质量变小;接种到玉米植株2周后,接种含毒菌株ZYFG11的玉米植株第2叶鞘病斑面积占30%、第3叶鞘病斑面积占10%,接种无毒菌株JSJH6的植株第2叶鞘和第3叶鞘病斑面积达100%,说明含毒菌株ZYFG11的致病力显著降低。综上,在玉米纹枯病菌中发现了一种新的减毒病毒RsHV4,能够影响寄主真菌的生物学特征和致病力,为玉米纹枯病的防治提供了潜在的病毒资源。

TRPV4在眼病理生理功能中的研究进展

瞬时受体电位香草醛受体4(TRPV4)是一种非选择性阳离子通道,负责感知细胞肿胀、温度、机械牵张、剪切应力和渗透压的变化,通过调节跨膜钙信号进而影响基因表达、细胞形态和细胞骨架等构建。TRPV4在全身广泛表达。在眼内,TRPV4在角膜、晶状体、睫状体、小梁网和视网膜等组织均有功能性表达。本文就TRPV4在眼内各个组织中的表达及生理病理功能方面进行阐述。随着TRPV4在眼部病理生理功能中的深入研究,TRPV4在角膜损伤修复、青光眼及视网膜血管生成方面可能成为潜在的新兴药物靶点,但仍需进一步的深入研究。

基于阵列声波测井估算碳酸盐岩储层岩石力学和地应力参数——以顺北4号带为例

地质力学分析在油气藏勘探开发过程中发挥着重要作用,顺北油气田中—下奥陶统一间房组—鹰山组储层地应力研究相对滞后。对比分析了顺北4号带下古生界中—下奥陶统一间房组—鹰山组碳酸盐岩不同类型储层的岩石力学和地应力特征,同时探讨了微观颗粒结构对碳酸盐岩储层地应力和岩石力学参数的控制作用,为顺北地区超深层碳酸盐岩储层评价提供了基础地质依据。基于弹簧组合模型,通过岩石力学实验和阵列声波测井确定了岩石力学和地应力特征,通过铸体薄片和X射线CT扫描表征碳酸盐岩的微观孔隙结构。研究结果表明:顺北4号带一间房组—鹰山组杨氏模量分布在50~89 GPa,抗压强度在99~136 MPa,泊松比在0.25~0.32;地层最大水平主应力为200~225 MPa,最小水平主应力为125~160 MPa。一间房组—鹰山组碳酸盐岩不同类型储层的岩石力学参数和地应力存在明显差异,从Ⅰ类、Ⅱ类、Ⅲ类到非储层段碳酸盐岩的杨氏模量、抗压强度和最大水平主应力明显增大,而泊松比和最小水平主应力变化不大。相比于一间房组—鹰山组泥晶灰岩,砂屑-生屑灰岩方解石颗粒较大,颗粒黏结程度降低,导致岩石抗压强度和杨氏模量减小;砂屑-生屑灰岩部分方解石颗粒呈次圆状或圆状,颗粒三维空间球度较大,颗粒之间咬合作用减弱,使得岩石的抗压强度和杨氏模量减小;砂屑-生屑灰岩存在大量的孔隙和裂缝,灰岩易沿着潜在的微裂缝面或者颗粒接触面摩擦滑动甚至破裂,同样导致岩石抗压强度和杨氏模量减小。此外,一间房组—鹰山组泥晶灰岩受热液作用影响,部分白云石交代为石英,导致泥晶灰岩的抗压强度和杨氏模量增加。岩石的宏观力学特征和岩石力学参数受岩石微观颗粒结构的制约,一间房组—鹰山组杨氏模量、抗压强度和最大水平主应力低值区即为顺北4号带优势储层发育区带。

P21活化激酶4在胃肠道间质瘤中的表达及意义

目的通过研究不同危险度胃肠道间质瘤(gastrointestinal stromal tumor,GIST)中P21活化激酶4(P21-activated kinase 4,PAK4)的表达量,探讨PAK4与GIST危险度的关系。方法选取2020年10月至2021年4月秦皇岛市第一医院病理诊断为GIST患者24例,根据共识将患者分为高危组、中危组、低危组,通过病理切片来确定不同危险度组的PAK4的表达量,并根据Callow计算方法对不同危险度组进行评分。结果PAK4随着GIST危险度增加表达升高,并且与正常对照组织及瘤旁组织相比,GIST中的PAK4表达量升高。结论PAK4表达量与GIST危险度相关,并且随着危险度升高,表达量也升高。

Lnc-PCIR通过调控PABPC4的泛素化促进喉癌细胞的增殖和迁移

目的:探究LncRNA RP11-214F16.8(Lnc-PCIR)通过调控多腺苷酸结合蛋白质4[poly(A)-binding protein cytoplasmic 4,PABPC4]的泛素化对喉癌细胞增殖、迁移的影响。方法:利用Human Protein Atlas、GEPIA数据库分析Lnc-PCIR、PABPC4在头颈鳞状细胞癌组织中的水平及患者的总体生存期。选取我院130例喉癌患者的癌及癌旁组织标本,RT-qPCR、Western blot检测组织Lnc-PCIR、PABPC4 mRNA和蛋白水平,并分析Lnc-PCIR水平与患者临床病理参数的关系。将HEP-2、TU212细胞进行不同转染,分为si-Lnc-NC组、si-Lnc-PCIR组、Lnc-NC组、Lnc-PCIR组、si-Lnc-PCIR+NC组、si-Lnc-PCIR+PABPC4组。CCK-8实验、EdU染色和Transwell实验检测细胞增殖和迁移能力;RT-qPCR检测细胞Lnc-PCIR、PABPC4 mRNA水平;HEP-2、TU212细胞分别经放线菌酮(cycloheximide,CHX)、MG132、ML364处理后,Western blot检测细胞PABPC4蛋白水平。20只裸鼠经皮下注射已转染的HEP-2细胞悬液,分为si-Lnc-NC组、si-Lnc-PCIR组;1个月后,测定移植瘤体积、质量和Lnc-PCIR、PABPC4蛋白水平。结果:数据库显示头颈鳞状细胞癌组织中Lnc-PCIR、PABPC4水平高于正常组织,且Lnc-PCIR、PABPC4水平与总体生存期呈负相关(均P<0.05)。与正常组织相比,患者喉癌组织中Lnc-PCIR、PABPC4 mRNA和蛋白水平升高,且Lnc-PCIR水平与患者性别、TNM分期、肿瘤分化程度、淋巴结转移相关(均P<0.05)。敲低Lnc-PCIR后,细胞Lnc-PCIR、PABPC4蛋白水平降低;细胞培养1 d、2 d、3 d后的OD值、EdU阳性率降低,细胞迁移数减少。而过表达PABPC4能部分逆转敲低Lnc-PCIR对细胞增殖和迁移的影响(均P<0.05);过表达Lnc-PCIR能降低细胞PABPC4泛素化水平(P<0.05)。敲低Lnc-PCIR能抑制小鼠移植瘤生长(P<0.05)。结论:Lnc-PCIR通过调控PABPC4的泛素化促进喉癌细胞的增殖和迁移。