Objective] This study aimed to discuss the key technology of in-vitro cul-ture for a new Vaccinium uliginosum cultivar Zishuijing. [Method] Through the screen-ing and optimization of sterilization method for explants,...Objective] This study aimed to discuss the key technology of in-vitro cul-ture for a new Vaccinium uliginosum cultivar Zishuijing. [Method] Through the screen-ing and optimization of sterilization method for explants, sampling time, multiplication, nursing and rooting culture, a matching clone propagation system was established for the new Vaccinium uliginosum cultivar Zishuijing. [Result] The explants were sterilized with 0.1% HgCl2 for 3 min; the differentiation and multiplication medium of Zishuijing was composed of WPM （modified）, 6-BA （1.0 mg/L） and ZT （1.0 MG/L）; the rootless tube seedlings were transplanted in organic matrix （sawdust∶bark∶peat=1∶1∶1） in September and cultured at air relative humidity of 80%-90% and temperature of 20-25 ℃, and after 50 d, the rooting rate reached 72.4%. [Conclusion] The key technol-ogy of in-vitro culture for the new Vaccinium uliginosum cultivar Zishuijing was estab-lished, thereby providing technical support for large-scale industrialized seedling pro-duction of Zishuijing.展开更多
基金Supported by Key Project of the National Twelfth-Five Year Research Program of China(2011BAD08B01-03)Funding Project of Department of Forestry of Heilongjiang Province(201004068-6)~~
文摘Objective] This study aimed to discuss the key technology of in-vitro cul-ture for a new Vaccinium uliginosum cultivar Zishuijing. [Method] Through the screen-ing and optimization of sterilization method for explants, sampling time, multiplication, nursing and rooting culture, a matching clone propagation system was established for the new Vaccinium uliginosum cultivar Zishuijing. [Result] The explants were sterilized with 0.1% HgCl2 for 3 min; the differentiation and multiplication medium of Zishuijing was composed of WPM （modified）, 6-BA （1.0 mg/L） and ZT （1.0 MG/L）; the rootless tube seedlings were transplanted in organic matrix （sawdust∶bark∶peat=1∶1∶1） in September and cultured at air relative humidity of 80%-90% and temperature of 20-25 ℃, and after 50 d, the rooting rate reached 72.4%. [Conclusion] The key technol-ogy of in-vitro culture for the new Vaccinium uliginosum cultivar Zishuijing was estab-lished, thereby providing technical support for large-scale industrialized seedling pro-duction of Zishuijing.
