Zinc oxide nanorods have been extensively studied for the specific killing of breast cancer(BC)cells,and their killing mechanism and anticancer effects have been initially demonstrated.However,systematic studies at th...Zinc oxide nanorods have been extensively studied for the specific killing of breast cancer(BC)cells,and their killing mechanism and anticancer effects have been initially demonstrated.However,systematic studies at the singlecell level are still necessary to explore cellular functions in detail.In this work,a hydrothermal method was used to synthesize zinc oxide nanorod arrays(ZnO NRs).Their effect on BC cells was demonstrated at single-cell resolution for the first time through microfluidic chips and a single-cell analysis platform.The inhibitory effects of ZnO NRs were observed.First,ZnO NRs suppressed cell proliferation and migration abilities.Moreover,Interferon-γ,Tumor Necrosis Factor-α,and Granzyme B in BC cells turned out to be antitumor instead of tumorigenic under ZnO NRs stimulation.Furthermore,ZnO NRs inhibition altered cellular functions and thus weakened intercellular and intercluster correlations.More importantly,MDA-MB-231 cells(strongly metastatic)showed much greater resistance to ZnO NRs than MCF-7 cells(nonmetastatic).The experiments complemented the findings at the single-cell level and provided a more comprehensive consideration of the potential risks and applications of ZnO NRs in breast cancer therapy,which is of great importance for biomedical research on nanomaterials.展开更多
基金EPSRC grants,Grant/Award Numbers:EP/R00661X/1,EP/P025021/1,EP/P025498/1the Fundamental Research Funds of Shandong University,Grant/Award Number:2020QNQT001+3 种基金Shandong University Integrated Research and Cultivation Project,Grant/Award Number:2022JC001National Natural Science Foundation of China,Grant/Award Numbers:32001018,62174101State Key Laboratory of Microbial Technology Open Projects Fund,Grant/Award Numbers:M2021-01,M2021-07Major Scientific and Technological Innovation Project of Shandong Province,Grant/Award Number:2021CXGC010603。
文摘Zinc oxide nanorods have been extensively studied for the specific killing of breast cancer(BC)cells,and their killing mechanism and anticancer effects have been initially demonstrated.However,systematic studies at the singlecell level are still necessary to explore cellular functions in detail.In this work,a hydrothermal method was used to synthesize zinc oxide nanorod arrays(ZnO NRs).Their effect on BC cells was demonstrated at single-cell resolution for the first time through microfluidic chips and a single-cell analysis platform.The inhibitory effects of ZnO NRs were observed.First,ZnO NRs suppressed cell proliferation and migration abilities.Moreover,Interferon-γ,Tumor Necrosis Factor-α,and Granzyme B in BC cells turned out to be antitumor instead of tumorigenic under ZnO NRs stimulation.Furthermore,ZnO NRs inhibition altered cellular functions and thus weakened intercellular and intercluster correlations.More importantly,MDA-MB-231 cells(strongly metastatic)showed much greater resistance to ZnO NRs than MCF-7 cells(nonmetastatic).The experiments complemented the findings at the single-cell level and provided a more comprehensive consideration of the potential risks and applications of ZnO NRs in breast cancer therapy,which is of great importance for biomedical research on nanomaterials.
