The efficiency of 7 treatment methods for breaking dormancy in zoysiagrass ( Zoysia japonica Steud.) seeds was synchronously compared. Two methods in which seeds soaking in 30% NaOH solution for 20 or 80 minutes were ...The efficiency of 7 treatment methods for breaking dormancy in zoysiagrass ( Zoysia japonica Steud.) seeds was synchronously compared. Two methods in which seeds soaking in 30% NaOH solution for 20 or 80 minutes were the optimum for breaking dormancy of seeds due to their simple and easy operation in techniques, economic and rapid effectiveness in performance and high germination rates (77.6% and 88.0% respectively).展开更多
[Objective] The paper was to analyze the expression and response mechanism of NCED gene of Zoysia japonica in initial infection stage of Rhizoctonia solani. [Method] The NCED gene fragments of Z. japonica‘Zenith＇wer...[Objective] The paper was to analyze the expression and response mechanism of NCED gene of Zoysia japonica in initial infection stage of Rhizoctonia solani. [Method] The NCED gene fragments of Z. japonica‘Zenith＇were screened and cloned on the basis of transcriptome data. The expression patterns of NCED gene in initial infection stage of R. solani（ 0-48 h） were obtained via real-time quantitative PCR. [Result] The expression level of NCED gene in stress treatment was higher than that without infection,which first increased then decreased with the extension of time,and reached the maximum at 36 h. Further detection of ABA level in‘Zenith＇roots showed that the change trend of ABA level within 0-48 h was consistent with the expression pattern of NCED gene,and flattened out after 48 h. [Conclusion] NCED gene regulates the synthesis of phytohormone ABA and probably further involves in plant disease resistance.展开更多
文摘The efficiency of 7 treatment methods for breaking dormancy in zoysiagrass ( Zoysia japonica Steud.) seeds was synchronously compared. Two methods in which seeds soaking in 30% NaOH solution for 20 or 80 minutes were the optimum for breaking dormancy of seeds due to their simple and easy operation in techniques, economic and rapid effectiveness in performance and high germination rates (77.6% and 88.0% respectively).
基金Supported by National High Technology Research and Development Program("863"Program) of China(2013AA102607)
文摘[Objective] The paper was to analyze the expression and response mechanism of NCED gene of Zoysia japonica in initial infection stage of Rhizoctonia solani. [Method] The NCED gene fragments of Z. japonica‘Zenith＇were screened and cloned on the basis of transcriptome data. The expression patterns of NCED gene in initial infection stage of R. solani（ 0-48 h） were obtained via real-time quantitative PCR. [Result] The expression level of NCED gene in stress treatment was higher than that without infection,which first increased then decreased with the extension of time,and reached the maximum at 36 h. Further detection of ABA level in‘Zenith＇roots showed that the change trend of ABA level within 0-48 h was consistent with the expression pattern of NCED gene,and flattened out after 48 h. [Conclusion] NCED gene regulates the synthesis of phytohormone ABA and probably further involves in plant disease resistance.
