[Objectives] This study was conducted to investigate the microscopic identification characteristics of Laportea bulbifera(Sieb. et Zucc.) Wedd. [Methods] The characteristics of L. bulbifera leaf, stem and powder were ...[Objectives] This study was conducted to investigate the microscopic identification characteristics of Laportea bulbifera(Sieb. et Zucc.) Wedd. [Methods] The characteristics of L. bulbifera leaf, stem and powder were identified by microscopy. [Results] The main microscopic identification characteristics of the tissue structures of L. bulbifera leaf and stem at different positions and L. bulbifera powder were determined. [Conclusions] The microscopic identification results are characteristic, and could serve as the identification basis of L. bulbifera. These results could provide references for the quality control of L. bulbifera.展开更多
[Objectives] This study was conducted to establish the quality control standards for Laportea bulbifera(Sieb. et Zucc.) Wedd. [Methods] Microscopic identification and thin layer chromatography were used to identify an...[Objectives] This study was conducted to establish the quality control standards for Laportea bulbifera(Sieb. et Zucc.) Wedd. [Methods] Microscopic identification and thin layer chromatography were used to identify and determine the moisture, total ash and extract contents. [Results] According to the microscopic results, the stem transection is round and has five longitudinal edges at which the cell wall is obviously thickened. Bristles can be observed. A series of collenchyma cells are arranged closely inside the epidermis. There are several rows of parenchyma cells. The vascular bundles are of different sizes;and the ray part is wide, and at the junction of the ray and the cortex, there are often scattered secretory cells. The center is a broad medulla. The powder is brown. There are many starch grains. The bristles are unicelluar, and have warty protrusions and expanded base. The fibers are bundled, and the cells are large. The epidermal cells are in the shape of an elongated spindle, in a mosaic arrangement, and the cell wall is slightly thickened. From the thin layer identification chromatograms of tested L. bulbifera, it can be seen that spots were observed at the same positions as the control medicinal material, and the durability of the method was good. The extract content should not be less than 12.00% by hot extraction with water as solvent, and not be less than 3.00% by cold extraction with ethanol as solvent. The moisture content should not be more than 16.50%. The total ash content should not be more than 14.00%. The acid-insoluble ash content should not be more than 2.50%. [Conclusions] The microscopic identification results are reliable and can be used as the identification basis of L. bulbifera. The thin layer identification method and the test results can provide a basis for quality control of L. bulbifera.展开更多
Using ISSR technique to analyze the diversity and genetic relationship of germplasm resources in 39 Prunus mume Sibe. et Zucc., the result showed that 10 primers were screened with high resolution from 51 primers, 120...Using ISSR technique to analyze the diversity and genetic relationship of germplasm resources in 39 Prunus mume Sibe. et Zucc., the result showed that 10 primers were screened with high resolution from 51 primers, 120 fragments were amplified, of which 98 were polymorphic loci, accounting for 81.67% of total. Tested materials were divided into 3 classes, as was fundamentally accorded with the traditional classification base on horticulture. There was no obvious difference in geographic relationship among the clustering results.展开更多
[Objective] To study the ecophysiological effects of low temperature stress on Actinidia arguta （Seib.et.Zucc.）, and provide a theoretical basis for the introduc- tion and breeding of cold resistant varieties. [Meth...[Objective] To study the ecophysiological effects of low temperature stress on Actinidia arguta （Seib.et.Zucc.）, and provide a theoretical basis for the introduc- tion and breeding of cold resistant varieties. [Method] A. arguta as the test material was processed at room temperature of 4 ℃ to determine the contents of chloro- phyll, water soluble sugar, water soluble protein, free proline, MDA, and antioxidant enzymes （POD activity, SOD activity, CAT activity） with the treatment at 25℃ as the control. [Results] The results indicated that with the prolonging of stress time, the content of chlorophyll was first increased and then decreased, and the content of MDA was increased; soluble sugar content of seedling leaf decreased gradually, while the soluble protein and proline content of seedling leaves were first increased and then decreased; the activities of SOD and POD were displayed a decline trend, meanwhile the activities of CAT was first increased and then decreased. [Conclu- sion] The various indicators showed that under low temperature stress, the chloro- phyll content decreased accompanied with membrane lipid peroxidation and MDA accumulation, and soluble sugar, soluble protein, free proline and antioxidant en- zymes play a protective role in different degrees.展开更多
通过琼脂平板扩散试验和双倍稀释试验,对青木香(Aristolochia debillis Sieb. et Zucc.)的石油醚提取物、乙酸乙酯提取物和甲醇提取物进行了抗细菌和抗真菌作用的研究,在三种提取物中,只有甲醇提取物对试验的所有革兰氏阳性和革兰氏阴...通过琼脂平板扩散试验和双倍稀释试验,对青木香(Aristolochia debillis Sieb. et Zucc.)的石油醚提取物、乙酸乙酯提取物和甲醇提取物进行了抗细菌和抗真菌作用的研究,在三种提取物中,只有甲醇提取物对试验的所有革兰氏阳性和革兰氏阴性细菌都表现出显著的抗菌作用,乙酸乙酯提取物只对测试的部分细菌显示出抗菌作用,石油醚提取物不具有抗菌活性.甲醇提取物比乙酸乙酯提取物具有更强的抗菌作用和更广的抗菌谱.三种提取物对所有试验的真菌都没有显示出抗菌作用.展开更多
目的探讨虎杖对湿热证2型糖尿病患者的临床疗效。方法140例患者随机分为对照组和观察组,每组70例,对照组给予常规治疗,观察组在对照组基础上加用虎杖颗粒,疗程8周。检测体质量、BMI、血糖指标(FBG、2 h PG、HbA_(1C)、GA)、血脂指标(TC...目的探讨虎杖对湿热证2型糖尿病患者的临床疗效。方法140例患者随机分为对照组和观察组,每组70例,对照组给予常规治疗,观察组在对照组基础上加用虎杖颗粒,疗程8周。检测体质量、BMI、血糖指标(FBG、2 h PG、HbA_(1C)、GA)、血脂指标(TC、TG、HDL-C、LDL-C、ApoA-I、ApoB、ApoA、ApoE、sdLDL-C)、肝功能指标(ALT、AST)、脂肪肝程度、TyG、HSI、中医证候评分和疗效变化。结果观察组中医证候总有效率高于对照组(P<0.01),脂肪肝程度更轻(P<0.01)。治疗后,观察组体质量、BMI、FBG、GA、TG、ApoE、TyG、HSI、ALT、中医证候评分降低(P<0.05,P<0.01),并且2 h PG、TyG、HSI、ALT、中医证候评分低于对照组(P<0.05,P<0.01)。结论虎杖可改善湿热证2型糖尿病患者糖脂代谢紊乱,值得临床推广应用。展开更多
气候变化背景下夏季极端降水发生频率增加,夏季水涝通过影响根系,从而影响树木生长和存活,对森林生态造成严重破坏。树木根系埋于地下,水涝后根系变化不易观测。研究目的是利用电阻抗图谱法非破坏性测定根系电阻抗特性,通过分析电阻抗...气候变化背景下夏季极端降水发生频率增加,夏季水涝通过影响根系,从而影响树木生长和存活,对森林生态造成严重破坏。树木根系埋于地下,水涝后根系变化不易观测。研究目的是利用电阻抗图谱法非破坏性测定根系电阻抗特性,通过分析电阻抗参数与根系生长生理指标的相关性,为快速了解水涝胁迫后白皮松地下根系的受害程度提供新方法。本研究以我国北方造林常用的白皮松四年生苗木为试验材料,于夏季进行水涝处理:i)对照(CK),正常管理;ii)连续水涝3周(CWL);iii)间歇水涝3周(IWL,水涝1周+排水1周,循环3次,总计水涝时长3周)。水涝后及后续恢复生长期对白皮松根系进行了电阻抗图谱测定及图谱归类分析(CLAFIC,Class-Featuring Information Compression)。对根系形态指标、非结构性碳水化合物、丙二醛含量进行了测定并分析与电阻抗参数的相关性。CWL和IWL处理下白皮松的根系形态指标较CK降低,且IWL处理下根系形态指标在恢复期显著低于CK和CWL处理,表明连续和间歇水涝3周均抑制了白皮松的根系生长,间歇水涝对根系的伤害较连续水涝更严重,与根系丙二醛含量更高相印证。CWL和IWL处理下白皮松根系的电阻抗图谱表现出明显的变化,电阻值(Re(Z))低于CK,容抗值(Im(Z))高于CK,恢复生长期间以上参数变化相反。CLAFIC归类结果显示CWL和IWL在水涝处理期间图谱相似比例最高,在恢复一个月后分别与CK相似比例较高。低频下阻抗损耗系数(δ)与根体积呈线性正相关,与淀粉含量呈线性负相关,表明水涝胁迫后白皮松苗木根系的阻抗损耗系数具有反映根大小和生理状态的潜力。展开更多
基金Supported by Guangxi Colleges and Universities Scientific Research Funding Project(NO.YB2014192)Guangxi Zhuang Medicine Quality Evaluation and Standard Research Project(NO.MZY2013023)+2 种基金Guangxi Scientific Research and Technological Development Program(GKG14124002-11-1)High-level-innovation Team and Outstanding Scholar Project of Guangxi Higher Education Institutes-Zhuang Medicine Basic and Clinical Innovation Team(GJR [2014]07)College Students'Innovation and Enterpreneurship Training Program of Guangxi University of Chinese Medicine(NO.2017DXS35)
文摘[Objectives] This study was conducted to investigate the microscopic identification characteristics of Laportea bulbifera(Sieb. et Zucc.) Wedd. [Methods] The characteristics of L. bulbifera leaf, stem and powder were identified by microscopy. [Results] The main microscopic identification characteristics of the tissue structures of L. bulbifera leaf and stem at different positions and L. bulbifera powder were determined. [Conclusions] The microscopic identification results are characteristic, and could serve as the identification basis of L. bulbifera. These results could provide references for the quality control of L. bulbifera.
基金Supported by Study on Quality Evaluation and Standards of Guangxi Yao Medicine(MZY2012015)Guangxi Key Laboratory of Zhuang Yao Medicine(GKJZ[2014]32)+2 种基金Zhuang Yao Medicine Collaborative Innovation Center(GJKY[2013]20)High-level-innovation Team and Outstanding Scholar Project of Guangxi Higher Education Institutions:Zhuang Medicine Foundation and Clinical Innovation Team(GJR[2014]07)Student Research Training Program of Guangxi University of Chinese Medicine(NO.2017DXS35).Wei WEI(1981-),male,P.R.China,experimentalist,devoted to research about Tradition Chinese medicine and ethnic drug
文摘[Objectives] This study was conducted to establish the quality control standards for Laportea bulbifera(Sieb. et Zucc.) Wedd. [Methods] Microscopic identification and thin layer chromatography were used to identify and determine the moisture, total ash and extract contents. [Results] According to the microscopic results, the stem transection is round and has five longitudinal edges at which the cell wall is obviously thickened. Bristles can be observed. A series of collenchyma cells are arranged closely inside the epidermis. There are several rows of parenchyma cells. The vascular bundles are of different sizes;and the ray part is wide, and at the junction of the ray and the cortex, there are often scattered secretory cells. The center is a broad medulla. The powder is brown. There are many starch grains. The bristles are unicelluar, and have warty protrusions and expanded base. The fibers are bundled, and the cells are large. The epidermal cells are in the shape of an elongated spindle, in a mosaic arrangement, and the cell wall is slightly thickened. From the thin layer identification chromatograms of tested L. bulbifera, it can be seen that spots were observed at the same positions as the control medicinal material, and the durability of the method was good. The extract content should not be less than 12.00% by hot extraction with water as solvent, and not be less than 3.00% by cold extraction with ethanol as solvent. The moisture content should not be more than 16.50%. The total ash content should not be more than 14.00%. The acid-insoluble ash content should not be more than 2.50%. [Conclusions] The microscopic identification results are reliable and can be used as the identification basis of L. bulbifera. The thin layer identification method and the test results can provide a basis for quality control of L. bulbifera.
基金Supported by Science and Technology Planning Project of Guang-dong Province (2007A020300002-6)~~
文摘Using ISSR technique to analyze the diversity and genetic relationship of germplasm resources in 39 Prunus mume Sibe. et Zucc., the result showed that 10 primers were screened with high resolution from 51 primers, 120 fragments were amplified, of which 98 were polymorphic loci, accounting for 81.67% of total. Tested materials were divided into 3 classes, as was fundamentally accorded with the traditional classification base on horticulture. There was no obvious difference in geographic relationship among the clustering results.
基金Supported by the Special Fund for Agro-scientific Research in the Public Interest (201303093)the Key Project for Agricultural Improved Variety Breeding of Shandong Province (Lunongliang[2016]No.6~~
文摘[Objective] To study the ecophysiological effects of low temperature stress on Actinidia arguta （Seib.et.Zucc.）, and provide a theoretical basis for the introduc- tion and breeding of cold resistant varieties. [Method] A. arguta as the test material was processed at room temperature of 4 ℃ to determine the contents of chloro- phyll, water soluble sugar, water soluble protein, free proline, MDA, and antioxidant enzymes （POD activity, SOD activity, CAT activity） with the treatment at 25℃ as the control. [Results] The results indicated that with the prolonging of stress time, the content of chlorophyll was first increased and then decreased, and the content of MDA was increased; soluble sugar content of seedling leaf decreased gradually, while the soluble protein and proline content of seedling leaves were first increased and then decreased; the activities of SOD and POD were displayed a decline trend, meanwhile the activities of CAT was first increased and then decreased. [Conclu- sion] The various indicators showed that under low temperature stress, the chloro- phyll content decreased accompanied with membrane lipid peroxidation and MDA accumulation, and soluble sugar, soluble protein, free proline and antioxidant en- zymes play a protective role in different degrees.
文摘通过琼脂平板扩散试验和双倍稀释试验,对青木香(Aristolochia debillis Sieb. et Zucc.)的石油醚提取物、乙酸乙酯提取物和甲醇提取物进行了抗细菌和抗真菌作用的研究,在三种提取物中,只有甲醇提取物对试验的所有革兰氏阳性和革兰氏阴性细菌都表现出显著的抗菌作用,乙酸乙酯提取物只对测试的部分细菌显示出抗菌作用,石油醚提取物不具有抗菌活性.甲醇提取物比乙酸乙酯提取物具有更强的抗菌作用和更广的抗菌谱.三种提取物对所有试验的真菌都没有显示出抗菌作用.
文摘目的探讨虎杖对湿热证2型糖尿病患者的临床疗效。方法140例患者随机分为对照组和观察组,每组70例,对照组给予常规治疗,观察组在对照组基础上加用虎杖颗粒,疗程8周。检测体质量、BMI、血糖指标(FBG、2 h PG、HbA_(1C)、GA)、血脂指标(TC、TG、HDL-C、LDL-C、ApoA-I、ApoB、ApoA、ApoE、sdLDL-C)、肝功能指标(ALT、AST)、脂肪肝程度、TyG、HSI、中医证候评分和疗效变化。结果观察组中医证候总有效率高于对照组(P<0.01),脂肪肝程度更轻(P<0.01)。治疗后,观察组体质量、BMI、FBG、GA、TG、ApoE、TyG、HSI、ALT、中医证候评分降低(P<0.05,P<0.01),并且2 h PG、TyG、HSI、ALT、中医证候评分低于对照组(P<0.05,P<0.01)。结论虎杖可改善湿热证2型糖尿病患者糖脂代谢紊乱,值得临床推广应用。
文摘气候变化背景下夏季极端降水发生频率增加,夏季水涝通过影响根系,从而影响树木生长和存活,对森林生态造成严重破坏。树木根系埋于地下,水涝后根系变化不易观测。研究目的是利用电阻抗图谱法非破坏性测定根系电阻抗特性,通过分析电阻抗参数与根系生长生理指标的相关性,为快速了解水涝胁迫后白皮松地下根系的受害程度提供新方法。本研究以我国北方造林常用的白皮松四年生苗木为试验材料,于夏季进行水涝处理:i)对照(CK),正常管理;ii)连续水涝3周(CWL);iii)间歇水涝3周(IWL,水涝1周+排水1周,循环3次,总计水涝时长3周)。水涝后及后续恢复生长期对白皮松根系进行了电阻抗图谱测定及图谱归类分析(CLAFIC,Class-Featuring Information Compression)。对根系形态指标、非结构性碳水化合物、丙二醛含量进行了测定并分析与电阻抗参数的相关性。CWL和IWL处理下白皮松的根系形态指标较CK降低,且IWL处理下根系形态指标在恢复期显著低于CK和CWL处理,表明连续和间歇水涝3周均抑制了白皮松的根系生长,间歇水涝对根系的伤害较连续水涝更严重,与根系丙二醛含量更高相印证。CWL和IWL处理下白皮松根系的电阻抗图谱表现出明显的变化,电阻值(Re(Z))低于CK,容抗值(Im(Z))高于CK,恢复生长期间以上参数变化相反。CLAFIC归类结果显示CWL和IWL在水涝处理期间图谱相似比例最高,在恢复一个月后分别与CK相似比例较高。低频下阻抗损耗系数(δ)与根体积呈线性正相关,与淀粉含量呈线性负相关,表明水涝胁迫后白皮松苗木根系的阻抗损耗系数具有反映根大小和生理状态的潜力。
