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Rapid Quantification of Chinese Medicine Zuo Jin Pill via RRLC
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作者 LIANG Xu ZHANG Xi +4 位作者 XU Zhi-xiu LIU Hui HE Ya-jun LUE Yong-hai ZHANG Wei-dong 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2010年第6期905-909,共5页
A rapid method based on rapid resolution liquid chromatography(RRLC) coupled with a diode array detector(DAD) was developed for the simultaneous determination of six major constituents(magnoflorine,jatrorrhizine,... A rapid method based on rapid resolution liquid chromatography(RRLC) coupled with a diode array detector(DAD) was developed for the simultaneous determination of six major constituents(magnoflorine,jatrorrhizine,coptisine,palmatine,berberine and evodiamine) in traditional Chinese medicine(TCM) Zuo Jin Pill(ZJP).The satisfactory chromatographic separation was carried on an Eclipse Plus C18 column(1.8 μm i.d.,150 mm×4.6 mm) by linear gradient elution with a mobile phase of acetonitrile-acetate buffer.All the calibration curves show good linearity(r2 0.9998).The detection limits and quantification limits ranged in 1.4―12 ng and 4.8―30 ng,respectively.The intra-and inter-day precisions were less than 0.63% with accuracies 98.60%―100.78%,and the recoveries were from 99.45% to 100.46%.Furthermore,hierarchical cluster analysis(HCA) was used to evaluate the variation of the herbal prescription.The results demonstrate that this analytical method is simple,sensitive and reliable for rapidly analyzing six major bioactive compounds in ZJPs and is helpful to comprehensively evaluating the quality of this TCM. 展开更多
关键词 zuo jin pill Rapid resolution liquid chromatography Simultaneous analysis Quality control Hierarchical cluster analysis
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左金丸通过Wnt/β-catenin信号通路对幽门螺杆菌感染的人胃癌细胞转移的影响 被引量:18
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作者 张彦博 李琦 +4 位作者 刘艳娜 叶乃菁 季青 柴妮 刘宣 《上海中医药大学学报》 CAS 2015年第2期44-47,52,共5页
目的:探讨左金丸通过抑制Wnt/β-catenin信号通路抗幽门螺杆菌(Helicobacter pylori,H.pylori)感染的人胃癌MKN45细胞转移的机制。方法:以H.pylori感染人胃癌MKN45细胞,采用CCK-8检测不同浓度左金丸醇提物对H.pylori感染的MKN45细胞生... 目的:探讨左金丸通过抑制Wnt/β-catenin信号通路抗幽门螺杆菌(Helicobacter pylori,H.pylori)感染的人胃癌MKN45细胞转移的机制。方法:以H.pylori感染人胃癌MKN45细胞,采用CCK-8检测不同浓度左金丸醇提物对H.pylori感染的MKN45细胞生长抑制作用。Transwell检测低、中、高剂量(25、50、100μg/ml)左金丸对H.pylori感染的MKN45细胞转移能力的影响;Western blot检测其对H.pylori感染的MKN45细胞核中的β-catenin蛋白表达;ELISA检测其对H.pylori感染的MKN45细胞培养上清液中MMP-7表达。结果:左金丸醇提物对H.pylori感染的MKN45细胞生长具有抑制作用,呈剂量依赖性。Transwell结果显示,与正常对照组比较,H.pylori感染后MKN45细胞转移能力显著增强(P<0.01);与H.pylori感染组比较,低、中、高剂量左金丸均能够明显抑制H.pylori诱导的MKN45细胞转移能力(P<0.01),呈剂量依赖性,且左金丸能够明显下调H.pylori诱导的β-catenin在细胞核的累积及其下游靶基因MMP-7蛋白表达(P<0.01)。结论:左金丸防治H.pylori诱发胃癌细胞转移的机制可能与通过Wnt/β-catenin信号通路抑制MMP-7表达有关。 展开更多
关键词 左金丸 胃癌 幽门螺杆菌 WNT/Β-CATENIN 转移
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