In-situ characterization of non-aqueous nano-dispersion systems（NANDS） by freeze-etching transmission electron microscope（FETEM） was reported.To improve just-for-once successive rate of specimen preparation and ge...In-situ characterization of non-aqueous nano-dispersion systems（NANDS） by freeze-etching transmission electron microscope（FETEM） was reported.To improve just-for-once successive rate of specimen preparation and get good characterization results,an improving specimen preparation method of freezing etching was developed.Size,distribution and morphology of NANDS were directly visualized.Some information of particle dispersion feature and particle density can also be obtained.Reproductivity of the FETEM characterization is excellent.Comparing with laser scattering method,which is liable to give positive error especially for small size particle anchoring disperser,FETEM characterization can give more accurate measurement of particle size.Moreover,FETEM can give dispersion feature of nanoparticle in non-aqueous medium.展开更多
Fine particles in seawater commonly form large porous aggregates. Aggregate density and settling velocity determine the behavior of this suspended particulate matter(SPM) within the water column.However, few studies o...Fine particles in seawater commonly form large porous aggregates. Aggregate density and settling velocity determine the behavior of this suspended particulate matter(SPM) within the water column.However, few studies of aggregate particles over a continental shelf have been undertaken. In our case study, properties of aggregate particles, including size and composition, over the continental shelf of the North Yellow Sea were investigated. During a scienti?c cruise in July 2016, in situ ef fective particle size distributions of SPM at 10 stations were measured, while temperature and turbidity measurements and samples of water were obtained from surface, middle, and bottom layers. Dispersed and inorganic particle size distributions were determined in the laboratory. The in situ SPM was divided into(1) small particles(<32 μm),(2) medium particles(32–256 μm) and(3) large particles(>256 μm). Large particles and medium particles dominated the total volume concentrations(VCs) of in situ SPM. After dispersion, the VCs of medium particles decreased to low values(<0.1 μL/L). The VCs of large particles in the surface and middle layers also decreased markedly, although they had higher peak values(0.1–1 μL/L). This suggests that almost all in situ medium particles and some large particles were aggregated, while other large particles were single particles. Correlation analysis showed that primary particles <32 μm in?uenced the formation of these aggregates. Microscopic examination revealed that these aggregates consisted of both organic and inorganic ?ne particles, while large particles were mucus-bound organic aggregates or individual plankton.The vertical distribution of coarser particles was clearly related to water strati?cation. Generally, medium aggregate particles were dominant in SPM of the bottom layer. A thermocline blocked resuspension of?ne material into upper layers, yielding low VCs of medium-sized aggregate particles in the surface layer.Abundant large biogenic particles were present in both surface and middle layers.展开更多
The principle of the technique of microscope laser light scattering spectroscopy, its development and applications to the studies on single intact cells, including our work on non-invasive in situ real time measuremen...The principle of the technique of microscope laser light scattering spectroscopy, its development and applications to the studies on single intact cells, including our work on non-invasive in situ real time measurements on single intact erythrocyte simultaneously at the levels of cellular, sub-cellular and intracellular molecules, are introduced.展开更多
根据2012年夏季和2016年冬季在南黄海中部利用原位激光粒度仪(laser in situ scattering and transmissometry,LISST)进行调查获得的实测数据,对两种平均粒径计算方法的结果进行比较研究,并对产生差别的原因进行分析。结果表明:无论在...根据2012年夏季和2016年冬季在南黄海中部利用原位激光粒度仪(laser in situ scattering and transmissometry,LISST)进行调查获得的实测数据,对两种平均粒径计算方法的结果进行比较研究,并对产生差别的原因进行分析。结果表明:无论在夏季和冬季,在温度跃层以上粒度分布以粗颗粒为主,两种计算方法差值较小,可以相互替代。在温盐跃层以下层位,细颗粒组分增加,导致二者计算差值变大。端元分析表明较高的平均粒径值倾向于造成平均粒径差值增大。矩值法强调了粗颗粒组分的贡献,而平均粒级法对细颗粒组分的贡献更加敏感,后者是更适用于将LISST观测数据应用于陆架海区现代沉积环境研究的粒径参数计算方法。本文为使用LISST在陆架海区进行现代沉积过程研究提供了参考。展开更多
基金Funded by National Natural Science Foundation of China(No.50572121) Key Pre-research Foundation of Weapon and Equipment(No. 9140A27010206JB35)
文摘In-situ characterization of non-aqueous nano-dispersion systems（NANDS） by freeze-etching transmission electron microscope（FETEM） was reported.To improve just-for-once successive rate of specimen preparation and get good characterization results,an improving specimen preparation method of freezing etching was developed.Size,distribution and morphology of NANDS were directly visualized.Some information of particle dispersion feature and particle density can also be obtained.Reproductivity of the FETEM characterization is excellent.Comparing with laser scattering method,which is liable to give positive error especially for small size particle anchoring disperser,FETEM characterization can give more accurate measurement of particle size.Moreover,FETEM can give dispersion feature of nanoparticle in non-aqueous medium.
基金Supported by the National Natural Science Foundation of China(No.41476045)the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA11030103)
文摘Fine particles in seawater commonly form large porous aggregates. Aggregate density and settling velocity determine the behavior of this suspended particulate matter(SPM) within the water column.However, few studies of aggregate particles over a continental shelf have been undertaken. In our case study, properties of aggregate particles, including size and composition, over the continental shelf of the North Yellow Sea were investigated. During a scienti?c cruise in July 2016, in situ ef fective particle size distributions of SPM at 10 stations were measured, while temperature and turbidity measurements and samples of water were obtained from surface, middle, and bottom layers. Dispersed and inorganic particle size distributions were determined in the laboratory. The in situ SPM was divided into(1) small particles(<32 μm),(2) medium particles(32–256 μm) and(3) large particles(>256 μm). Large particles and medium particles dominated the total volume concentrations(VCs) of in situ SPM. After dispersion, the VCs of medium particles decreased to low values(<0.1 μL/L). The VCs of large particles in the surface and middle layers also decreased markedly, although they had higher peak values(0.1–1 μL/L). This suggests that almost all in situ medium particles and some large particles were aggregated, while other large particles were single particles. Correlation analysis showed that primary particles <32 μm in?uenced the formation of these aggregates. Microscopic examination revealed that these aggregates consisted of both organic and inorganic ?ne particles, while large particles were mucus-bound organic aggregates or individual plankton.The vertical distribution of coarser particles was clearly related to water strati?cation. Generally, medium aggregate particles were dominant in SPM of the bottom layer. A thermocline blocked resuspension of?ne material into upper layers, yielding low VCs of medium-sized aggregate particles in the surface layer.Abundant large biogenic particles were present in both surface and middle layers.
文摘The principle of the technique of microscope laser light scattering spectroscopy, its development and applications to the studies on single intact cells, including our work on non-invasive in situ real time measurements on single intact erythrocyte simultaneously at the levels of cellular, sub-cellular and intracellular molecules, are introduced.
文摘根据2012年夏季和2016年冬季在南黄海中部利用原位激光粒度仪(laser in situ scattering and transmissometry,LISST)进行调查获得的实测数据,对两种平均粒径计算方法的结果进行比较研究,并对产生差别的原因进行分析。结果表明:无论在夏季和冬季,在温度跃层以上粒度分布以粗颗粒为主,两种计算方法差值较小,可以相互替代。在温盐跃层以下层位,细颗粒组分增加,导致二者计算差值变大。端元分析表明较高的平均粒径值倾向于造成平均粒径差值增大。矩值法强调了粗颗粒组分的贡献,而平均粒级法对细颗粒组分的贡献更加敏感,后者是更适用于将LISST观测数据应用于陆架海区现代沉积环境研究的粒径参数计算方法。本文为使用LISST在陆架海区进行现代沉积过程研究提供了参考。
