Interferon regulatory factor 7 plays a crucial role in the innate immune response.However,whether interferon regulatory factor 7-mediated signaling contributes to Parkinson's disease remains unknown.Here we report...Interferon regulatory factor 7 plays a crucial role in the innate immune response.However,whether interferon regulatory factor 7-mediated signaling contributes to Parkinson's disease remains unknown.Here we report that interferon regulatory factor 7 is markedly up-regulated in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced mouse model of Parkinson's disease and co-localizes with microglial cells.Both the selective cyclic guanosine monophosphate adenosine monophosphate synthase inhibitor RU.521 and the stimulator of interferon genes inhibitor H151 effectively suppressed interferon regulatory factor 7 activation in BV2 microglia exposed to 1-methyl-4-phenylpyridinium and inhibited transformation of mouse BV2 microglia into the neurotoxic M1 phenotype.In addition,si RNA-mediated knockdown of interferon regulatory factor 7 expression in BV2 microglia reduced the expression of inducible nitric oxide synthase,tumor necrosis factorα,CD16,CD32,and CD86 and increased the expression of the anti-inflammatory markers ARG1 and YM1.Taken together,our findings indicate that the cyclic guanosine monophosphate adenosine monophosphate synthase-stimulator of interferon genes-interferon regulatory factor 7 pathway plays a crucial role in the pathogenesis of Parkinson's disease.展开更多
本文中应用全部PECVD工艺沉积a-SiOx:H、a-SiNx:H,和a-SiOx:H/a-SiNx:H叠层系统,比较了不同钝化膜对多晶硅太阳能电池发射极和背面钝化效果,应用FGA、RTP等热处理方法对钝化膜进行处理,重点讨论了FGA(Forming gas annealing)温度和时间...本文中应用全部PECVD工艺沉积a-SiOx:H、a-SiNx:H,和a-SiOx:H/a-SiNx:H叠层系统,比较了不同钝化膜对多晶硅太阳能电池发射极和背面钝化效果,应用FGA、RTP等热处理方法对钝化膜进行处理,重点讨论了FGA(Forming gas annealing)温度和时间的长短对钝化的影响。结果表明:低温FGA对只有单面钝化膜的硅片钝化效果不明显,而在800℃下FGA有明显作用,而且钝化效果随着时间的增加呈现出先增大后减小然后再增大的现象;退火后降温环境中是否有H和降温时间对钝化效果有很大的影响,但是对于双面膜无论FGA温度高低对钝化都有帮助,文中对上述现象做了合理的解释。最后利用双面叠层钝化膜经过FGA处理后得到的多晶硅片的少子寿命达到14.2μs,比镀膜之前的3.0μs提高了11.2μs,使多晶硅太阳能电池暗电压Voc达到630mV。展开更多
Ara h 2是花生主要过敏原之一,为开发食物中Ara h 2过敏原成分的快速检测方法,减少因误食导致花生过敏事件的发生,该研究采用鼠源单克隆抗体作为捕获抗体、兔源多克隆抗体作为检测抗体,通过棋盘法优化抗体工作浓度,建立了一种检测花生...Ara h 2是花生主要过敏原之一,为开发食物中Ara h 2过敏原成分的快速检测方法,减少因误食导致花生过敏事件的发生,该研究采用鼠源单克隆抗体作为捕获抗体、兔源多克隆抗体作为检测抗体,通过棋盘法优化抗体工作浓度,建立了一种检测花生过敏原Ara h 2的间接双抗夹心化学发光酶免疫分析法,并对该方法的灵敏度、准确度、精密度和特异性进行评价。该方法的检出限为1.085 ng/mL,线性范围为3.12~200 ng/mL,添加回收率为78.30%~94.39%,批内和批间变异系数均小于10%,且特异性良好,与其他常见食物过敏原无交叉反应。该方法与相同抗体所建立的间接双抗夹心酶联免疫吸附测定(enzyme-linked immunosorbent assay, ELISA)方法相比,在灵敏度上表现出一定优势。该研究开发的化学发光酶免疫分析法可对花生食品生产过程中和消费前的Ara h 2过敏原成分检测提供可靠的技术支持。展开更多
基金supported by the National Natural Science Foundation of China,Nos.82171429,81771384a grant from Wuxi Municipal Health Commission,No.1286010241190480(all to YS)。
文摘Interferon regulatory factor 7 plays a crucial role in the innate immune response.However,whether interferon regulatory factor 7-mediated signaling contributes to Parkinson's disease remains unknown.Here we report that interferon regulatory factor 7 is markedly up-regulated in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced mouse model of Parkinson's disease and co-localizes with microglial cells.Both the selective cyclic guanosine monophosphate adenosine monophosphate synthase inhibitor RU.521 and the stimulator of interferon genes inhibitor H151 effectively suppressed interferon regulatory factor 7 activation in BV2 microglia exposed to 1-methyl-4-phenylpyridinium and inhibited transformation of mouse BV2 microglia into the neurotoxic M1 phenotype.In addition,si RNA-mediated knockdown of interferon regulatory factor 7 expression in BV2 microglia reduced the expression of inducible nitric oxide synthase,tumor necrosis factorα,CD16,CD32,and CD86 and increased the expression of the anti-inflammatory markers ARG1 and YM1.Taken together,our findings indicate that the cyclic guanosine monophosphate adenosine monophosphate synthase-stimulator of interferon genes-interferon regulatory factor 7 pathway plays a crucial role in the pathogenesis of Parkinson's disease.
文摘本文中应用全部PECVD工艺沉积a-SiOx:H、a-SiNx:H,和a-SiOx:H/a-SiNx:H叠层系统,比较了不同钝化膜对多晶硅太阳能电池发射极和背面钝化效果,应用FGA、RTP等热处理方法对钝化膜进行处理,重点讨论了FGA(Forming gas annealing)温度和时间的长短对钝化的影响。结果表明:低温FGA对只有单面钝化膜的硅片钝化效果不明显,而在800℃下FGA有明显作用,而且钝化效果随着时间的增加呈现出先增大后减小然后再增大的现象;退火后降温环境中是否有H和降温时间对钝化效果有很大的影响,但是对于双面膜无论FGA温度高低对钝化都有帮助,文中对上述现象做了合理的解释。最后利用双面叠层钝化膜经过FGA处理后得到的多晶硅片的少子寿命达到14.2μs,比镀膜之前的3.0μs提高了11.2μs,使多晶硅太阳能电池暗电压Voc达到630mV。
文摘Ara h 2是花生主要过敏原之一,为开发食物中Ara h 2过敏原成分的快速检测方法,减少因误食导致花生过敏事件的发生,该研究采用鼠源单克隆抗体作为捕获抗体、兔源多克隆抗体作为检测抗体,通过棋盘法优化抗体工作浓度,建立了一种检测花生过敏原Ara h 2的间接双抗夹心化学发光酶免疫分析法,并对该方法的灵敏度、准确度、精密度和特异性进行评价。该方法的检出限为1.085 ng/mL,线性范围为3.12~200 ng/mL,添加回收率为78.30%~94.39%,批内和批间变异系数均小于10%,且特异性良好,与其他常见食物过敏原无交叉反应。该方法与相同抗体所建立的间接双抗夹心酶联免疫吸附测定(enzyme-linked immunosorbent assay, ELISA)方法相比,在灵敏度上表现出一定优势。该研究开发的化学发光酶免疫分析法可对花生食品生产过程中和消费前的Ara h 2过敏原成分检测提供可靠的技术支持。
