Genetic transformation is widely used to improve target traits and to study gene function in wheat.However,transformation efficiency depends on the physiological status of the recipient genotype and that is affected b...Genetic transformation is widely used to improve target traits and to study gene function in wheat.However,transformation efficiency depends on the physiological status of the recipient genotype and that is affected by several factors including powdery mildew(PM)infection.The widely used recipient variety Fielder is very susceptible to PM.Therefore,it would be beneficial to develop PM resistant derivatives with high regeneration ability for use in genetic transformation.In the present study PM resistant lines CB037 and Pm97033 carrying genes Pm21 and PmV,respectively,were backcrossed to Fielder with selection for PM resistance.Five lines,NT89,NT90,NT154,and WT48 with Pm21 and line FL347 with PmV were developed,identified by molecular markers and genomic in situ hybridization(GISH)or fluorescent in situ hybridization(FISH),and further subjected to detailed assessment of agronomic traits and regeneration ability following genetic transformation capacity.Lines FL347,WT48,NT89 and NT154 assessed as being equal to,or superior,to Fielder in regeneration and transformation ability are recommended as suitable materials for the replacement of Fielder for wheat gene transfer and genome editing study.展开更多
Objective： To investigate the mechanism of carcinogenesis, invasion and metastasis. Methods： The expressions of adhesive molecule and adhesive structure in v-k-ras transformed normal rat kidney cells （KNRK） were d...Objective： To investigate the mechanism of carcinogenesis, invasion and metastasis. Methods： The expressions of adhesive molecule and adhesive structure in v-k-ras transformed normal rat kidney cells （KNRK） were detected with a variety of molecular biological techniques, including cell culture, immunofluorescence labeling, electron microscopy, polyacrylamide gel electrophoresis, and protein blotting, and compared with normal rat kidney （NRK） cells. Results： The significantly shortened doubling time, remarkably active proliferation ability in soft agar, and invasive growth in the abdomen of nude rat, demonstrated the malignant biological behaviors of KNRK cells. In KNRK cells, the adhesive molecules, P-cadherin, α and β catenin, actin, and adhesive structures, the adhesive junction and gap junction, were all abnormally expressed. And cell aggregation was significantly decreased. The aggregation ability disappeared at 20℃, and became active with a suitable amount of calcium solution. Conclusion： Following the transfection of virus K-ras gene, normal cells were transformed into malignant cells. In early stage of cancer, the variation of adhesive ability may be one of the vital factors underlying tumorigenesis, invasion and metastasis.展开更多
Based on the embeddedness theory,this paper analyzes the impact of network embedding features and types on the transformation performance of foundry enterprises.Through the investigation and analysis of more than 200 ...Based on the embeddedness theory,this paper analyzes the impact of network embedding features and types on the transformation performance of foundry enterprises.Through the investigation and analysis of more than 200 foundry enterprises in Zhejiang Province,it is found that network relationship embedding and structure embedding have positive impacts on transformation of foundry enterprises.Professional embedding and technical embedding have a positive effect in the transformation of foundry enterprises,and knowledge absorption ability has a positive adjustment role in the transformation performance of network embedding foundry enterprises.展开更多
For young teachers,the ability to transform teaching materials and the ability to control teaching is a basic teaching regulation and control ability. Through a case teaching of function limits in the mathematics cour...For young teachers,the ability to transform teaching materials and the ability to control teaching is a basic teaching regulation and control ability. Through a case teaching of function limits in the mathematics course,this article studied how to explore deep into the content of textbooks,use the textbooks in a creative and proper manner,help students better understand and master the content of teaching,improve students ’autonomous learning ability,and promote comprehensive improvement of students’ knowledge,ability and caliber.展开更多
基金supported by the National Natural Science Foundation of China(31971945).
文摘Genetic transformation is widely used to improve target traits and to study gene function in wheat.However,transformation efficiency depends on the physiological status of the recipient genotype and that is affected by several factors including powdery mildew(PM)infection.The widely used recipient variety Fielder is very susceptible to PM.Therefore,it would be beneficial to develop PM resistant derivatives with high regeneration ability for use in genetic transformation.In the present study PM resistant lines CB037 and Pm97033 carrying genes Pm21 and PmV,respectively,were backcrossed to Fielder with selection for PM resistance.Five lines,NT89,NT90,NT154,and WT48 with Pm21 and line FL347 with PmV were developed,identified by molecular markers and genomic in situ hybridization(GISH)or fluorescent in situ hybridization(FISH),and further subjected to detailed assessment of agronomic traits and regeneration ability following genetic transformation capacity.Lines FL347,WT48,NT89 and NT154 assessed as being equal to,or superior,to Fielder in regeneration and transformation ability are recommended as suitable materials for the replacement of Fielder for wheat gene transfer and genome editing study.
基金This work was supported by the National Natural Sciences Foundation of China (No. 30371624).
文摘Objective： To investigate the mechanism of carcinogenesis, invasion and metastasis. Methods： The expressions of adhesive molecule and adhesive structure in v-k-ras transformed normal rat kidney cells （KNRK） were detected with a variety of molecular biological techniques, including cell culture, immunofluorescence labeling, electron microscopy, polyacrylamide gel electrophoresis, and protein blotting, and compared with normal rat kidney （NRK） cells. Results： The significantly shortened doubling time, remarkably active proliferation ability in soft agar, and invasive growth in the abdomen of nude rat, demonstrated the malignant biological behaviors of KNRK cells. In KNRK cells, the adhesive molecules, P-cadherin, α and β catenin, actin, and adhesive structures, the adhesive junction and gap junction, were all abnormally expressed. And cell aggregation was significantly decreased. The aggregation ability disappeared at 20℃, and became active with a suitable amount of calcium solution. Conclusion： Following the transfection of virus K-ras gene, normal cells were transformed into malignant cells. In early stage of cancer, the variation of adhesive ability may be one of the vital factors underlying tumorigenesis, invasion and metastasis.
基金Humanities and Social Sciences Foundation of the Ministry of Education,China(No.17YJA630087)
文摘Based on the embeddedness theory,this paper analyzes the impact of network embedding features and types on the transformation performance of foundry enterprises.Through the investigation and analysis of more than 200 foundry enterprises in Zhejiang Province,it is found that network relationship embedding and structure embedding have positive impacts on transformation of foundry enterprises.Professional embedding and technical embedding have a positive effect in the transformation of foundry enterprises,and knowledge absorption ability has a positive adjustment role in the transformation performance of network embedding foundry enterprises.
基金Supported by Education Research and Reform Program of Tianjin Agricultural University in 2018"A Practical Study of the Teaching Ability Cultivation of Young Teachers in Colleges and Universities"(2018-B-35).
文摘For young teachers,the ability to transform teaching materials and the ability to control teaching is a basic teaching regulation and control ability. Through a case teaching of function limits in the mathematics course,this article studied how to explore deep into the content of textbooks,use the textbooks in a creative and proper manner,help students better understand and master the content of teaching,improve students ’autonomous learning ability,and promote comprehensive improvement of students’ knowledge,ability and caliber.
