Serum proteins differentially expressed in gestational diabetes mellitus assessed using isobaric tag for relative and absolute quantitation proteomics

BACKGROUND As a well-known fact to the public,gestational diabetes mellitus(GDM)could bring serious risks for both pregnant women and infants.During this important investigation into the linkage between GDM patients and their altered expression in the serum,proteomics techniques were deployed to detect the differentially expressed proteins(DEPs)of in the serum of GDM patients to further explore its pathogenesis,and find out possible biomarkers to forecast GDM occurrence.METHODS Subjects were divided into GDM and normal control groups according to the IADPSG diagnostic criteria.Serum samples were randomly selected from four cases in each group at 24-28 wk of gestation,and the blood samples were identified by applying iTRAQ technology combined with liquid chromatography-tandem mass spectrometry.Key proteins and signaling pathways associated with GDM were identified by bioinformatics analysis,and the expression of key proteins in serum from 12 wk to 16 wk of gestation was further verified using enzyme-linked immunosorbent assay (ELISA).RESULTS Forty-seven proteins were significantly differentially expressed by analyzing the serum samples between the GDMgravidas as well as the healthy ones. Among them, 31 proteins were found to be upregulated notably and the rest16 proteins were downregulated remarkably. Bioinformatic data report revealed abnormal expression of proteinsassociated with lipid metabolism, coagulation cascade activation, complement system and inflammatory responsein the GDM group. ELISA results showed that the contents of RBP4, as well as ANGPTL8, increased in the serumof GDM gravidas compared with the healthy ones, and this change was found to initiate from 12 wk to 16 wk ofgestation.CONCLUSION GDM symptoms may involve abnormalities in lipid metabolism, coagulation cascade activation, complementsystem and inflammatory response. RBP4 and ANGPTL8 are expected to be early predictors of GDM.

Effects of Electric Stimulations Applied during Absolute Refractory Period on Cardiac Function of Rabbits with Heart Failure

The effects of electric currents applied during absolute refractory period （ARP） on the cardiac function of rabbits with heart failure due to myocardial infarction （MI）, and the safety of this method were investigated. Thirty rabbits were randomly assigned equally to 3 groups： sham-operated group, LV-anterior wall cardiac contractility modulation （LV-CCM） group, and septum-CCM （S-CCM） group. A thoracotomy was performed on all the rabbits. Electric pulses were delivered during the ARP on the anterior wall of left ventricle in CCM group and in the septum in S-CCM group, respectively. The left ventricular systolic pressure （LVSP） and maximum positive left ventricular pressure change （＋dp/dtmax）, heart rates, ventricular tachycardia, ventricular fibrillation were observed. It was found that, as compared with the baseline, LVSP, and ＋dp/dtmax were significantly increased, on average, by 15.2% and 19.5% in LV-CCM group （P〈0,05）, and by 8.5% and 10.8% in S-CCM group （P〈0.05）. LVEDP was significantly decreased and -dp/dtmx increased both in LV-CCM group and S-CCM group （P〈0.05）. CCM had no effect on heart rate and induced no arrhythmia in short time. It is concluded that electric currents delivered during the ARP could significantly enhance the contractility of myocardium safely, suggesting that CCM stimulation is a novel potent method for contractility modulation.

Determination of optimal period of absolute encoders with single track cyclic gray code

Low cost and miniaturized rotary encoders are important in automatic and precise production. Presented here is a code called Single Track Cyclic Gray Code (STCGC) that is an image etched on a single circular track of a rotary encoder disk read by a group of even spread reading heads to provide a unique codeword for every angular position and features such that every two adjacent words differ in exactly one component, thus avoiding coarse error. The existing construction or combination methods are helpful but not sufficient in determining the period of the STCGC of large word length and the theoretical approach needs further development to extend the word length. Three principles, such as the seed combination, short code removal and ergodicity examination were put forward that suffice determination of the optimal period for such absolute rotary encoders using STCGC with even spread heads. The optimal periods of STCGC in 3 through 29 bit length were determined and listed.

Measurement and Analysis of Vibration Effect of Free-falling Corner Cube Driving Mechanism in Free Fall Absolute Gravimeter

The vibration interference of the reference corner cube runs through the free flight process of the free-falling corner cube,which is superimposed on the whole laser interference fringes.Thus,it is necessary to solve the interference fringes with the entire fringe to analyze the quantitative influence of vibration on gravity measurements.

Euler Product Expressions of Absolute Tensor Products of Dirichlet L-Functions

In this paper, we calculate the absolute tensor square of the Dirichlet L-functions and show that it is expressed as an Euler product over pairs of primes. The method is to construct an equation to link primes to a series which has the factors of the absolute tensor product of the Dirichlet L-functions. This study is a generalization of Akatsuka’s theorem on the Riemann zeta function, and gives a proof of Kurokawa’s prediction proposed in 1992.

Effects of Electric Stimulations Applied During Absolute Refractory Period on Ventricular Muscle from Rabbits

Objectives To investigate the influences of electric signals applied during absolute refractory period (ARP) on the contractility of isolated papillary muscle from rabbits. Methods Papillary muscle was exercised from the right ventricle and was paced at 1 Hz. Biphasic square wave current pulse was delivered during the absolute refractory period (called CCM) in isolated, superfused, isometrically contractility rabbit papillary muscle. The peak tension (PT) of papillary muscle, as well as maximum positive tension change ( + dT/dtmax), were observed. Results Compared with the baseline, both PT and + dT/dtmax significantly increased during CCM stimulation by 18.2% and 21.4% respectively (P < 0. 05) . In addition, PT increased significantly with one or two beats following CCM signal application and reached a. new steady state level after a few beats. Once the CCM signals were turned off, the PT returned to the approximately baseline level ( P < 0. 05). Moreover, the effect of CCM on PT was dose - response to voltage. The obvious effect was at higher voltage. No effect was observed at lower voltage. Conclusions Electric signals delivered during the absolute refractory period can rapidly enhance the contractility of myocardium, which suggests that CCM signal is a novel potent method for contractility modulation.

A Novel Inverse-Free Neurodynamic Approach for Solving Absolute Value Equations

We propose a novel inverse-free neurodynamic approach (NIFNA) for solving absolute value equations (AVE). The NIFNA guarantees global convergence and notably improves convergence speed by achieving fixed-time convergence. To validate the theoretical findings, numerical simulations are conducted, demonstrating the effectiveness and efficiency of the proposed NIFNA.

Periodic dividends and capital injections for a spectrally negative Lévy risk process under absolute ruin

The spectrally negative Lévy risk model with random observation times is considered in this paper,in which both dividends and capital injections are made at some independent Poisson observation times.Under the absolute ruin,the expected discounted dividends and the expected discounted capital injections are discussed.We also study the joint Laplace transforms including the absolute ruin time and the total dividends or the total capital injections.All the results are expressed in scale functions.

Absolute Wall Thickness Measurement of Conducting Plates Using Pulsed Eddy Currents

The method using pulsed eddy currents to determine the thickness of a conduction plate is extended to enable the simultaneous measurement of the plate thickness and material properties. For optimal performance, a probe must be designed depending on the thickness range that should be accessible. The need for a calibration of the material properties of a conducting plate to enable the measurement of its thickness has been removed. All that is needed is a probe with known dimensions and suitable hardware to create a current pulse and measure a transient magnetic induction.

Insulin-induced severe thyrotoxic periodic paralysis:A case report

BACKGROUND Thyrotoxic periodic paralysis(TPP)is an endocrine emergency caused by thyrotoxicosis,manifesting mainly as periodic myasthenia and hypokalemia,and posing a serious threat to the patient's health.Fatigue,strenuous exercise,alcohol abuse,high carbohydrate intake and insulin injections are common triggers of paralysis.This article reports a case of severe TPP induced by insulin injection,elucidates the characteristics and pathogenesis of the disease,analyses the risk factors for triggering TPP,and hopefully provides more clinical data for TPP patients.CASE SUMMARY A 38-year-old Asian man presented to the emergency department with a oneweek history of limb weakness and worsening half-day.His medical history included poorly controlled type 2 diabetes and he had been switched to Aspart50 a week earlier.He was alert and oriented with upper extremity strength grade 3 and lower extremity strength grade 1.Emergency department tests showed hypokalemia of 1.6 mmol/L.The paramedics administered 1.5 g of potassium intravenously,followed by 4.0 g orally.Weakness in the arms and legs improved.He was referred to endocrinology where he was diagnosed with Graves'disease,with suboptimal control and insulin injections possibly causing TPP.We stopped his insulin and he was discharged with a potassium level of 4.0 mmol/L.CONCLUSION Insulin is a trigger for TPP and should be avoided in patients with hyperthyroidism.Early recognition and treatment of TPP is crucial,especially in patients presenting with hypokalemic periodic paralysis.

Effects of Electrical Stimulation During Absolute Refractory Period on Contraction and Relaxation of Cardiomyocytes from Normal Guinea - pigs

Objectives To investi-gate the effect of electrical stimulation during absolute refractory period on contraction and relaxation of ventricular myocytes from normal guinea - pigs. Methods The guinea - pig ventricular myocytes were obtained by enzyme digesting, and the extent of its contraction and relaxation and Ca2+ transient were recorded through the motion edge detection system. Results (1) The contracting extent of guinea - pig ventricular myocytes increased 15.45±6.48% , and the peak velocity of shorting (contraction) and the peak veloci ty of relengthening (relaxation) increased 15.97 ± 8.37% and 21. 63±8. 06% respectively (n = 10); (2) The extent of ventricular myocytes fluorescence ra tio(360/380) (F360/F380) increased 22. 55±9. 08%, and the peak velocity of ventricular myocytes F360 /F380 increased 36. 75 ± 9. 77% and 23. 62±4. 47% during shorting and relengthening respectively (n = 6) . Conclusions Appropriate electrical stimulation may strengthen the contracting and relaxing function of normal guinea - pig ventricular myocytes.

Research of the wave measurement using GPS absolute velocity estimation technology

This paper analyzed the existing methods of wave measurement, and described the advantages of GPS applied in measuring the wave. The equations of absolute velocity estimation were discussed, focusing on two methods with Doppler shill. The error sources and their effect on velocity estimation were analyzed. Then, some tests were carried on to simulate dynamic velocity determination using static data Based on the high-frequency carrier-phase derived Doppler observations, the velocity has been estimated to the precision of 1 cm/s or so, even to the mm/s level. And with the receiver generated Doppler measurements, the precision can reach 3 - 15 cm/s.

New criterion for delay-dependent absolute stability of Lurie system with interval time-varying delay

The delay-dependent absolute stability for a class of Lurie systems with interval time-varying delay is studied. By employing an augmented Lyapunov functional and combining a free-weighting matrix approach and the reciprocal convex technique, an improved stability condition is derived in terms of linear matrix inequalities （LMIs）. By retaining some useful terms that are usually ignored in the derivative of the Lyapunov function, the proposed sufficient condition depends not only on the lower and upper bounds of both the delay and its derivative, but it also depends on their differences, which has wider application fields than those of present results. Moreover, a new type of equality expression is developed to handle the sector bounds of the nonlinear function, which achieves fewer LMIs in the derived condition, compared with those based on the convex representation. Therefore, the proposed method is less conservative than the existing ones. Simulation examples are given to demonstrate the validity of the approach.

MULTIVARIATE ABSOLUTE DEGREE OF GREY INCIDENCE BASED ON DISTRIBUTION CHARACTERISTICS OF POINTS

The analysis result of absolute degree of grey incidence for multivariate time series is often inconsistent with the qualitative analysis. To overcome this shortage, a multivariate absolute degree of grey incidence based on distribution characteristics of points is proposed. Based on the geometric description of multivariate time se- ries, the neighborhood extrema are extracted in the different regions, and a characteristic point set is constructed. Then according to the distribution of the characteristic point set, a characteristic point sequence reflecting the ge- ometric features of multivariate time series is obtained. The incidence analysis between multivariate time series is transformed into the relational analysis between characteristic point sequences, and a grey incidence model is established. The model possesses the properties of translational invariance, transpose and rank transform invari- ance, and satisfies the grey incidence analysis axioms. Finally, two cases are studied and the results prove the ef- fectiveness of the model.

Absolute Exponential Stability of Generalized Dynamical Neural Networks

This paper investigates the absolute exponential stability of generalized neural networks with a general class of partially Lipschitz continuous and monotone increasing activation functions. The main obtained result is that if the interconnection matrix T of the neural system satisfies that - T is an H matrix with nonnegative diagonal elements, then the neural system is absolutely exponentially stable(AEST). The Hopfield network, Cellular neural network and Bidirectional associative memory network are special cases of the network model considered in this paper. So this work gives some improvements to the previous ones.

极性强化词的语义走向——以absolutely为例

以absolutely为例,结合共时与历时主观化框架,对英语极性强化词的语义演化路径加以追踪,发现abso-lutely的意义体现了客观义<主观义<交互主观义的单向演进趋势。对照汉语对应成分"极",可以发现英汉对应范畴在语义演化路径上存在一定的共通性。

Empirical Bayes Absolute Error Loss Estimation for Parameter of One-Side Truncation Distribution Families

In this paper we propose an absolute error loss EB estimator for parameter of one-side truncation distribution families. Under some conditions we have proved that the convergence rates of its Bayes risk is o, where 0<λ,r≤1,Mn≤lnln n (for large n),Mn→∞ as n→∞.

Review of low timing jitter mode-locked fiber lasers and applications in dual-comb absolute distance measurement

Passively mode-locked fiber lasers emit femtosecond pulse trains with excellent short-term stability. The quantum-limited timing jitter of a free running femtosecond erbium-doped fiber laser working at room temperature is considerably below one femtosecond at high Fourier frequency. The ultrashort pulse train with ultralow timing jitter enables absolute time-of-flight measurements based on a dual-comb implementation, which is typically composed of a pair of optical frequency combs generated by femtosecond lasers. Dead-zone-free absolute distance measurement with sub-micrometer precision and kHz update rate has been routinely achieved with a dual-comb configuration, which is promising for a number of precision manufacturing applications, from large step-structure measurements prevalent in microelectronic profilometry to three coordinate measurements in large-scale aerospace manufacturing and shipbuilding. In this paper, we first review the sub-femtosecond precision timing jitter characterization methods and approaches for ultralow timing jitter mode-locked fiber laser design. Then, we provide an overview of the state-of-the-art dual-comb absolute ranging technology in terms of working principles, experimental implementations, and measurement precisions. Finally, we discuss the impact of quantum-limited timing jitter on the dual-comb ranging precision at a high update rate. The route to highprecision dual-comb range finder design based on ultralow jitter femtosecond fiber lasers is proposed.

Absolute monocyte and lymphocyte count prognostic score for patients with gastric cancer

AIM:To measure the prognostic significance of absolute monocyte count/absolute lymphocyte count prognostic score(AMLPS) in patients with gastric cancer.METHODS:We retrospectively examined the combination of absolute monocyte count(AMC) and absolute lymphocyte count(ALC) as prognostic variables in a cohort of 299 gastric cancer patients who underwent surgical resection between 2006 and 2013 and were followed at a single institution.Both AMC and ALC were dichotomized into two groups using cut-off points determined by receiving operator characteristic curve analysis.An AMLPS was generated,which stratified patients into three risk groups:low risk(both low AMC and high ALC),intermediate risk(either high AMC or low ALC),and high risk(both high AMC and low ALC).The primary objective of the study was to validate the impact of AMLPS on both disease-free survival(DFS) and overall survival(OS),and the second objective was to assess the AMLPS as an independent prognostic factor for survival in comparison with known prognostic factors.RESULTS:Using data from the entire cohort,the most discriminative cut-off values of AMC and ALC selected on the receiver operating characteristic curve were 672.4/μL and 1734/μL for DFS and OS.AMLPS risk groups included 158(52.8%) patients in the lowrisk,128(42.8%) in the intermediate-risk,and 13(4.3%) in the high-risk group.With a median followup of 37.2 mo(range:1.7-91.4 mo),five-year DFS rates in the low-,intermediate-,and high-risk groups were 83.4%,78.7%,and 19.8%,respectively.And fiveyear OS rates in the low-,intermediate-,and high-risk groups were 89.3%,81.1%,and 14.4%,respectively.On multivariate analysis performed with patient- and tumor-related factors,we identified AMLPS,age,and pathologic tumor-node-metastasis stage as the most valuable prognostic factors impacting DFS and OS.CONCLUSION:AMLPS identified patients with a poor DFS and OS,and it was independent of age,pathologic stage,and various inflammatory markers.

LMI approach for absolute stability of general neutral type Lurie indirect control systems

This paper deals with the problem of the absolute stability for general neutral type Lurie indirect control systems by Lyapunov method and linear matrix inequality （LMI） technique. Delay-dependent sufficient conditions for the absolute stability are derived and expressed as the feasibility problem of LMI, which can be easily solved by Matlab Toolbox. Finally, some examples are provide to demonstrate the effectiveness of proposed method.