Achyranthes bidentata and Achyranthes aspera are saponin and steroid rich medicinal plants, used extensively for therapeutic treatments in Traditional Chinese Medicine(TCM) and Ayurveda. A. bidentata is reported to be...Achyranthes bidentata and Achyranthes aspera are saponin and steroid rich medicinal plants, used extensively for therapeutic treatments in Traditional Chinese Medicine(TCM) and Ayurveda. A. bidentata is reported to be one of the rare and extensively exploited medicinal plant species that face the issue of being endangered.Finding qualitative substitute with identical phyto-constituents contributing to similar composition and pharmacological benefits will help in reducing the burden of exploitation of the natural habitats of such plants.In the present study, a comparative metabolite analysis of the whole drug and specific tissues isolated by laser micro-dissection(LMD) was carried out for both the selected species, by use of ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UHPLC-QTOF MS). The results of the study indicate that the cortex and the medullary ray tissues are rich in their content of steroidal and saponin constituents such as(25 S)-inokosterone-20,22-acetonide, ginsenoside Ro, bidentatoside II and achyranthoside B.Metabolite profiling of the whole tissues of both the species indicates presence of identical constituents. Thus,it is inferred that A. bidentata and A. aspera can be used as qualitative substitutes for each other.展开更多
目的基于NF-κB信号通路探讨牛膝总皂苷治疗膝骨性关节炎滑膜炎症的机制。方法选择SPF级SD大鼠60只,随机分成对比组、模型组、牛膝总皂苷低剂量组、牛膝总皂苷中剂量组、牛膝总皂苷高剂量组,每组12只。除对比组外,其他各组大鼠在关节两...目的基于NF-κB信号通路探讨牛膝总皂苷治疗膝骨性关节炎滑膜炎症的机制。方法选择SPF级SD大鼠60只,随机分成对比组、模型组、牛膝总皂苷低剂量组、牛膝总皂苷中剂量组、牛膝总皂苷高剂量组,每组12只。除对比组外,其他各组大鼠在关节两侧进针,从第1天开始每间隔2 d分别注射0.3 mL 5%木瓜蛋白酶水溶液到大鼠膝关节腔内,比较5组大鼠关节炎症指标。结果与对比组比较,模型组的膝关节肿胀程度较严重,miR-19α的基因表达水平较低,而SOX9、NF-κB的基因水平表达较高,使用牛膝总皂苷的3组,根据剂量的增加肿胀水平依次降低,miR-19α基因表达水平依次增高,而SOX9以及NF-κB的基因表达水平和蛋白表达水平依次降低。结论牛膝总皂苷能减轻骨关节炎的炎症症状,并且能通过上调miR-19α的表达抑制SOX9、NF-κB的表达水平,保护软骨细胞的凋亡。展开更多
文摘Achyranthes bidentata and Achyranthes aspera are saponin and steroid rich medicinal plants, used extensively for therapeutic treatments in Traditional Chinese Medicine(TCM) and Ayurveda. A. bidentata is reported to be one of the rare and extensively exploited medicinal plant species that face the issue of being endangered.Finding qualitative substitute with identical phyto-constituents contributing to similar composition and pharmacological benefits will help in reducing the burden of exploitation of the natural habitats of such plants.In the present study, a comparative metabolite analysis of the whole drug and specific tissues isolated by laser micro-dissection(LMD) was carried out for both the selected species, by use of ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UHPLC-QTOF MS). The results of the study indicate that the cortex and the medullary ray tissues are rich in their content of steroidal and saponin constituents such as(25 S)-inokosterone-20,22-acetonide, ginsenoside Ro, bidentatoside II and achyranthoside B.Metabolite profiling of the whole tissues of both the species indicates presence of identical constituents. Thus,it is inferred that A. bidentata and A. aspera can be used as qualitative substitutes for each other.
文摘目的基于NF-κB信号通路探讨牛膝总皂苷治疗膝骨性关节炎滑膜炎症的机制。方法选择SPF级SD大鼠60只,随机分成对比组、模型组、牛膝总皂苷低剂量组、牛膝总皂苷中剂量组、牛膝总皂苷高剂量组,每组12只。除对比组外,其他各组大鼠在关节两侧进针,从第1天开始每间隔2 d分别注射0.3 mL 5%木瓜蛋白酶水溶液到大鼠膝关节腔内,比较5组大鼠关节炎症指标。结果与对比组比较,模型组的膝关节肿胀程度较严重,miR-19α的基因表达水平较低,而SOX9、NF-κB的基因水平表达较高,使用牛膝总皂苷的3组,根据剂量的增加肿胀水平依次降低,miR-19α基因表达水平依次增高,而SOX9以及NF-κB的基因表达水平和蛋白表达水平依次降低。结论牛膝总皂苷能减轻骨关节炎的炎症症状,并且能通过上调miR-19α的表达抑制SOX9、NF-κB的表达水平,保护软骨细胞的凋亡。
