本研究通过多光谱技术深入探讨盐浓度(离子强度)对酸性绿9(acid green 9,AG9)和酸性蓝9(acid blue 9,AB9)与牛血清白蛋白(bovine serum albumin,BSA)之间相互作用的影响。运用德拜休克尔极限理论定量分析,计算得到真实的吉布斯自由能变...本研究通过多光谱技术深入探讨盐浓度(离子强度)对酸性绿9(acid green 9,AG9)和酸性蓝9(acid blue 9,AB9)与牛血清白蛋白(bovine serum albumin,BSA)之间相互作用的影响。运用德拜休克尔极限理论定量分析,计算得到真实的吉布斯自由能变化(ΔG^0_I→0)和BSA的阴离子受体腔内有效电荷(Z_B)。配体AG9/AB9所带电荷Z_C的值为负,Z_B的值为正,意味着相反电荷之间离子对的形成,即AG9/AB9的负电荷与BSA的净正电荷结合腔(位点-Ⅰ)相结合,表明BSA的局部电荷主导BSA-AG9/AB9体系的相互作用,而不是它的整体或表面电荷。随着盐浓度的增加,这两个体系的熵变逐渐由正值变为负值,且焓变逐渐增大,使ΔG^0几乎不变而发生焓熵补偿。最终其热力学参数由ΔH^0<0、ΔS^0>0(静电力)在高盐浓度下转变为ΔH0<0、ΔS0<0(非静电力),表明AG9/AB9与BSA相互作用时,它们的移动或局部运动由于主导力的转变而受阻。展开更多
A novel semiconducting oligo（9-fluorenylideneacetic acid） （OFYA） with good redox activity and stability was successfully electrosynthesized by direct anodic oxidation of 9-fluorenylideneacetic acid （FYA） in CH2C...A novel semiconducting oligo（9-fluorenylideneacetic acid） （OFYA） with good redox activity and stability was successfully electrosynthesized by direct anodic oxidation of 9-fluorenylideneacetic acid （FYA） in CH2C12 containing boron trifluoride diethyl etherate （BFEE） as the supporting electrolyte. The as-formed OFYA film was readily soluble in dimethyl sulfoxide and tetrahydrofuran, and partly soluble in water, alcohol, acetonitrile and acetone. FT-IR and 1H NMR spectra, together with computational results proved that FYA was probably polymerized through the coupling at C（2） and C（7） positions. Further, OFYA was a typical green light-emitter with maximal emission at 555 nm and its fluorescence quantum yield was distinctly improved in comparison with that of the monomer. The oligomer was also studied by UV-vis spectroscopy, MALDL-TOF mass spectrometry, and thermal analysis, respectively.展开更多
文摘本研究通过多光谱技术深入探讨盐浓度(离子强度)对酸性绿9(acid green 9,AG9)和酸性蓝9(acid blue 9,AB9)与牛血清白蛋白(bovine serum albumin,BSA)之间相互作用的影响。运用德拜休克尔极限理论定量分析,计算得到真实的吉布斯自由能变化(ΔG^0_I→0)和BSA的阴离子受体腔内有效电荷(Z_B)。配体AG9/AB9所带电荷Z_C的值为负,Z_B的值为正,意味着相反电荷之间离子对的形成,即AG9/AB9的负电荷与BSA的净正电荷结合腔(位点-Ⅰ)相结合,表明BSA的局部电荷主导BSA-AG9/AB9体系的相互作用,而不是它的整体或表面电荷。随着盐浓度的增加,这两个体系的熵变逐渐由正值变为负值,且焓变逐渐增大,使ΔG^0几乎不变而发生焓熵补偿。最终其热力学参数由ΔH^0<0、ΔS^0>0(静电力)在高盐浓度下转变为ΔH0<0、ΔS0<0(非静电力),表明AG9/AB9与BSA相互作用时,它们的移动或局部运动由于主导力的转变而受阻。
文摘A novel semiconducting oligo（9-fluorenylideneacetic acid） （OFYA） with good redox activity and stability was successfully electrosynthesized by direct anodic oxidation of 9-fluorenylideneacetic acid （FYA） in CH2C12 containing boron trifluoride diethyl etherate （BFEE） as the supporting electrolyte. The as-formed OFYA film was readily soluble in dimethyl sulfoxide and tetrahydrofuran, and partly soluble in water, alcohol, acetonitrile and acetone. FT-IR and 1H NMR spectra, together with computational results proved that FYA was probably polymerized through the coupling at C（2） and C（7） positions. Further, OFYA was a typical green light-emitter with maximal emission at 555 nm and its fluorescence quantum yield was distinctly improved in comparison with that of the monomer. The oligomer was also studied by UV-vis spectroscopy, MALDL-TOF mass spectrometry, and thermal analysis, respectively.
