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An optimized recording method to characterize biophysical and pharmacological properties of acid-sensing ion channel
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作者 李爱 司文 +2 位作者 胡新武 刘长金 曹晓华 《Neuroscience Bulletin》 SCIE CAS CSCD 2008年第3期160-165,共6页
Objective To re-confirm and characterize the biophysical and pharmacological properties of endogenously expressed human acid-sensing ion channel 1a (hASIC1a) current in HEK293 cells with a modified perfusion methods... Objective To re-confirm and characterize the biophysical and pharmacological properties of endogenously expressed human acid-sensing ion channel 1a (hASIC1a) current in HEK293 cells with a modified perfusion methods. Methods With cell floating method, which is separating the cultured cell from coverslip and putting the cell in front of perfusion tubing, whole cell patch clamp technique was used to record hASICla currents evoked by low pH external solution. Results Using cell floating method, the amplitude of hASICla currents activated by pH 5.0 in HEK293 cells is twice as large as that by the conventional method where the cells remain attached to coverslip. The time to reach peak at two different recording conditions is (21±5) ms and (270±25) ms, respectively. Inactivation time constants are (496±23) ms and (2284±120) ms, respectively. The cell floating method significantly increases the amiloride potency of block on hASIC 1 a [IC50 is (3.4± 1.1 ) μmol/L and (2.4± 0.9) μmol/L, respectively]. Both recording methods have similar pH activation ECs0 (6.6±0.6, 6.6±0.7, respectively). Conclusion ASICs channel activation requires fast exchange of extracellular solution with the different pH values. With cell floating method, the presence of hASIC la current was re-confirmed and the biophysical and pharmacological properties of hASIC la channel in HEK293 cells was precisely characterized. This method could be used to study all ASICs and other ligand-gated channels that require fast extracellular solution exchange. 展开更多
关键词 acid-sensing ion channel patch-clamp recording PH
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Identification and Function of Acid-sensing Ion Channels in RAW 264.7 Macrophage Cells 被引量:2
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作者 Lan NI Peng FANG +4 位作者 Zhuang-li HU Hai-yun ZHOU Jian-guo CHEN Fang WANG You JIN 《Current Medical Science》 SCIE CAS 2018年第3期436-442,共7页
Activation of acid-sensing ion channels (ASICs) plays an important role in neuroinflammation. Macrophage recruitment to the sites of inflammation is an essential step in host defense. ASIC1 and ASIC3 have been repor... Activation of acid-sensing ion channels (ASICs) plays an important role in neuroinflammation. Macrophage recruitment to the sites of inflammation is an essential step in host defense. ASIC1 and ASIC3 have been reported to mediate the endocytosis and maturation of bone marrow derived macrophages. However, the expression and inflammation-related functions of ASICs in RAW 264.7 cells, another common macrophage, are still elusive. In the present study, we first demonstrated the presence of ASIC 1, ASIC2a and ASIC3 in RAW 264.7 macrophage cell line by using reverse transcriptase polymerase chain reaction (RT-PCR), Western blotting and immunofluorescence experiments. The non-specific ASICs inhibitor amiloride and specific homomeric ASICla blocker PcTxl reduced the production of iNOS and COX-2 by LPS-induced activating RAW 264.7 cells. Furthermore, not only amiloride but also PcTxl inhibited the migration and LPS-induced apoptosis of RAW 264.7 cells. Taken together, our findings suggest that ASICs promote the inflammatory response and apoptosis of RAW 264.7 cells, and ASICs may serve as a potential novel target for immunological disease therapy. 展开更多
关键词 acid-sensing ion channels (asics) RAW 264.7 cells INFLAMMATion apoptosis MIGRATion
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Involvement of Acid-sensing Ion Channel 1a in Functions of Cultured Human Retinal Pigment Epithelial Cells 被引量:1
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作者 谭健 许益聘 +1 位作者 刘广鹏 叶信海 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2013年第1期137-141,共5页
In the retina, pH fluctuations may play an important role in adapting retinal responses to different light intensities and are involved in the fine tuning of visual perception. Acidosis occurs in the subretinal space ... In the retina, pH fluctuations may play an important role in adapting retinal responses to different light intensities and are involved in the fine tuning of visual perception. Acidosis occurs in the subretinal space (SRS) under pathological conditions such as age-related macular degeneration (AMD). Although it is well known that many transporters in the retinal pigment epithelium (RPE) cells can maintain pH homeostasis efficiently, other receptors in RPE may also be involved in sensing acidosis, such as acid-sensing ion channels (ASICs). In this study, we investigated whether ASICla was ex- pressed in the RPE cells and whether it was involved in the function of these cells. Real-time RT-PCR and Western blotting were used to analyze the ASICla expression in ARPE-19 cells during oxidative stress induced by hydrogen peroxide (H202). Furthermore, inhibition or over-expression of ASICla in RPE cells was obtained using inhibitors (amiloride and PCTxl) or by the transfection of cDNA encod- ing hASICla. Cell viability was determined by using the MTT assay. The real-time RT-PCR and West- ern blotting results showed that both the mRNA and protein of ASICla were expressed in RPE cells. In- hibition of ASICs by amiloride in normal RPE cells resulted in cell death, indicating that ASICs play an important physiological role in RPE cells. Furthermore, over-expression of ASICla in RPE cells pro- longed cell survival under oxidative stress induced by H2O2. In conclusion, ASICla is functionally expressed in RPE cells and may play an important role in the physiological function of RPE cells by pro-tecting them from oxidative stress. 展开更多
关键词 acid-sensing ion channel la retinal pigment epithelium AMILORIDE PCTxl hydrogen peroxide
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Ischemic postconditioning protects against ischemic brain injury by up-regulation of acid-sensing ion channel 2a 被引量:5
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作者 Wang-sheng Duanmu Liu Cao +3 位作者 Jing-yu Chen Hong-fei Ge Rong Hu Hua Feng 《Neural Regeneration Research》 SCIE CAS CSCD 2016年第4期641-645,共5页
Ischemic postconditioning renders brain tissue tolerant to brain ischemia,thereby alleviating ischemic brain injury.However,the exact mechanism of action is still unclear.In this study,a rat model of global brain isch... Ischemic postconditioning renders brain tissue tolerant to brain ischemia,thereby alleviating ischemic brain injury.However,the exact mechanism of action is still unclear.In this study,a rat model of global brain ischemia was subjected to ischemic postconditioning treatment using the vessel occlusion method.After 2 hours of ischemia,the bilateral common carotid arteries were blocked immediately for 10 seconds and then perfused for 10 seconds.This procedure was repeated six times.Ischemic postconditioning was found to mitigate hippocampal CA1 neuronal damage in rats with brain ischemia,and up-regulate acid-sensing ion channel 2a expression at the m RNA and protein level.These findings suggest that ischemic postconditioning up-regulates acid-sensing ion channel 2a expression in the rat hippocampus after global brain ischemia,which promotes neuronal tolerance to ischemic brain injury. 展开更多
关键词 neural regeneration brain injury ischemic brain injury acid-sensing ion channels neuroprotection ischemic postconditioning neuroprotection protein expression neuronal density ischemic tolerance molecular mechanism gene expression nerve regeneration
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Does closure of acid-sensing ion channels reduce ischemia/reperfusion injury in the rat brain?
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作者 Jie Wang Yinghui Xu +5 位作者 Zhigang Lian Jian Zhang Tingzhun Zhu Mengkao Li Yi Wei Bin Dong 《Neural Regeneration Research》 SCIE CAS CSCD 2013年第13期1169-1179,共11页
Acidosis is a common characteristic of brain damage. Because studies have shown that permeable Ca2+-acid-sensing ion channels can mediate the toxic effects of calcium ions, they have become new targets against pain a... Acidosis is a common characteristic of brain damage. Because studies have shown that permeable Ca2+-acid-sensing ion channels can mediate the toxic effects of calcium ions, they have become new targets against pain and various intracranial diseases. However, the mechanism associated with expression of these channels remains unclear. This study sought to observe the expression characteristics of permeable Ca2+-acid-sensing ion channels during different reperfusion inflows in rats after cerebral ischemia. The rat models were randomly divided into three groups: adaptive ischemia/reperfusion group, one-time ischemia/reperfusion group, and severe cerebral ischemic injury group. Western blot assays and immunofluorescence staining results exhibited that when compared with the one-time ischemia/reperfusion group, acid-sensing ion channel 3 and Bcl-x/I expression decreased in the adaptive ischemia/reperfusion group. Calmodulin expression was lowest in the adaptive ischemia/reperfusion group. Following adaptive reperfusion, common carotid artery flow was close to normal, and the pH value improved. Results verified that adaptive reperfusion following cerebral ischemia can suppress acid-sensing ion channel 3 expression, significantly reduce Ca2+ influx, inhibit calcium overload, and diminish Ca2+ toxicity. The effects of adaptive ischemia/reperfusion on suppressing cell apoptosis and relieving brain damage were better than that of one-time ischemia/reperfusion. 展开更多
关键词 neural regeneration brain injury acid-sensing ion channel 3 cerebral ischemia REPERFUSion apoptosis CALMODULIN calcium overload nerve cells grants-supported paper NEUROREGENERATion
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Effect of Activation of the Ca2+-Permeable Acid-Sensing Ion Channel 1a on Acid-Induced Vascular Endothelial Cell Injury of Henoch-Schönlein Purpura Children
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作者 Qidi Peng Liping Yuan +2 位作者 Yan Bo Xiaoyan Guo Hu Bo 《Open Journal of Pediatrics》 2016年第4期324-332,共9页
Acidosis in local environment plays a critical role in cell injury. One key mediator of acidosis-induced cell injury is the acid-sensing ion channels (ASICs), particularly ASIC1a. Herein, we investigated the role of A... Acidosis in local environment plays a critical role in cell injury. One key mediator of acidosis-induced cell injury is the acid-sensing ion channels (ASICs), particularly ASIC1a. Herein, we investigated the role of ASIC1a in acid-induced vascular endothelial cell injury of Henoch-Schonlein purpura (HSP) children. Acid-induced ASIC1a, Calpain and Calcineurin expression in vascular endothelial cells pretreated with IgA1 isolated from HSP were detected by real time quantitative polymerase chain reaction and western blot methods, respectively. Cell cytotoxicity was measured by interleukin-8 and nitric oxide production with ELISA. The results showed acid-induced ASIC1a, Calpain and Calcineurin expression in cells increased, especially at PH6.5. The cytotoxicity of vascular endothelial cells was increased by extracellular acidosis. Moreover non-specific or specific blockers of ASIC1a, Amiloride and PcTX-1 could remarkably decrease these parameters. These findings show that increased [Ca<sup>2+</sup>]i, mediated via ASIC1a, might contribute to acid-induced vascular endothelial cell injury of HSP. 展开更多
关键词 acid-sensing ion channels (asics) Vascular Endothelial Cell Henoch-Schönlein Purpura (HSP)
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siRNA沉默ASIC1a基因对佐剂性关节炎大鼠关节软骨细胞凋亡的影响研究 被引量:4
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作者 江晟 陈飞虎 +5 位作者 陈寸知 荣超 胡伟 吴繁荣 葛金芳 唐杰 《中国临床药理学与治疗学》 CAS CSCD 2012年第3期256-262,共7页
目的:探讨小分子干扰RNA(siRNA)技术诱导佐剂性关节炎(adjuvant-induced arthritis,AA)大鼠关节软骨细胞中ASIC1a表达沉默对细胞凋亡的影响。方法:通过化学合成法合成特异性荧光短链ASIC1asiRNA-FAM,使用Lipo-fectamine 2000转染试剂盒... 目的:探讨小分子干扰RNA(siRNA)技术诱导佐剂性关节炎(adjuvant-induced arthritis,AA)大鼠关节软骨细胞中ASIC1a表达沉默对细胞凋亡的影响。方法:通过化学合成法合成特异性荧光短链ASIC1asiRNA-FAM,使用Lipo-fectamine 2000转染试剂盒将ASIC1asiRNA转染入关节软骨细胞,采用荧光显微镜、流式细胞术、实时荧光定量PCR(q-RT-PCR)及WesternBlot法检测siRNA转染效率及其对ASIC1amRNA和蛋白表达的抑制作用。同时采用An-nexin-V/PI流式细胞术检测各组细胞凋亡情况。结果:ASIC1asiRNA能成功转入软骨细胞,转染后AA大鼠关节软骨细胞中ASIC1amRNA表达显著低于对照组(P<0.01),最大抑制率为85.4%;Western Blot结果显示,转染特异性siRNA后ASIC1a蛋白表达明显低于对照组(P<0.01)。Annexin-V/PI流式细胞术结果表明,与模型组相比,siRNA-3转染引起ASIC1a表达沉默后AA大鼠软骨细胞凋亡明显减少。结论:siRNA介导的AA大鼠关节软骨细胞ASIC1a表达沉默模型是研究酸敏感离子通道对软骨细胞代谢影响的可靠模型,siRNA-3转染对胞外酸化刺激条件下AA大鼠关节软骨细胞凋亡的保护作用可能与其调节的表达有关。 展开更多
关键词 关节软骨细胞 酸敏感离子通道(asics) 阳离子脂质体 表达沉默 细胞凋亡
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大鼠背根神经节酸感受离子通道(ASICs)的药理学特性研究 被引量:5
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作者 艾平 刘振伟 +2 位作者 戴薇薇 张树卓 郑建全 《中国药理学通报》 CAS CSCD 北大核心 2007年第1期33-37,共5页
目的研究大鼠背根神经节(DRG)细胞酸感受离子通道(ASICs)的电生理学和药理学特性。方法应用全细胞膜片钳技术,在急性分散的成年大鼠DRG细胞上记录并分析由不同浓度H+(降低pH值)诱发的ASICs电流。结果在266个大鼠DRG神经元上记录到由H+... 目的研究大鼠背根神经节(DRG)细胞酸感受离子通道(ASICs)的电生理学和药理学特性。方法应用全细胞膜片钳技术,在急性分散的成年大鼠DRG细胞上记录并分析由不同浓度H+(降低pH值)诱发的ASICs电流。结果在266个大鼠DRG神经元上记录到由H+诱发的3种不同类型的ASICs电流,分别为ASIC1样电流(n=66,24·8%)、ASIC2样电流(n=81,30·4%)和ASIC3样电流(n=119,44·7%)。ASIC1样电流具有快速失活成份,衰减快;ASIC2样电流具有稳态失活成份,衰减十分缓慢;而ASIC3样电流具有快速失活与稳态失活双相成份。三者均不具有整流现象。此3种电流对细胞外H+表现出不同的敏感性,H+诱发电流的pH50分别是:ASIC1-like,pH5·82;ASIC2-like,pH5·18;ASIC3-like,pH6·24。氨氯吡咪以浓度依赖性方式可逆性阻断大鼠DRG神经元的ASICs,对3种ASICs电流的抑制效应差异有显著性。其IC50分别为:ASIC1-like,19·86μmol·L-1;ASIC2-like,42·73μmol·L-1;ASIC3-like,27·91μmol·L-1。结论成年大鼠DRG神经元细胞上表达了3种不同类型的ASICs,且其在表达率、H+敏感性、失敏以及氨氯吡咪敏感性等方面差异均有显著性。 展开更多
关键词 酸感受离子通道 背根神经节 全细胞膜片钳 氨氯吡咪
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酸环境对过敏性紫癜患儿血清IgA1诱导血管内皮细胞ASIC1a表达的影响及机制 被引量:6
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作者 闫波 袁丽萍 +2 位作者 彭启迪 房功思 戴寒晶 《基础医学与临床》 CSCD 2017年第12期1674-1680,共7页
目的初步探讨酸性环境对过敏性紫癜(HSP)患儿血清Ig A1诱导血管内皮细胞酸敏感离子通道1a(ASIC1a)表达的影响及ASIC1a的调控作用。方法体外培养的人皮肤微血管内皮细胞经过处理后,荧光定量PCR检测血管内皮细胞ASIC1a、destrin及α-SM m... 目的初步探讨酸性环境对过敏性紫癜(HSP)患儿血清Ig A1诱导血管内皮细胞酸敏感离子通道1a(ASIC1a)表达的影响及ASIC1a的调控作用。方法体外培养的人皮肤微血管内皮细胞经过处理后,荧光定量PCR检测血管内皮细胞ASIC1a、destrin及α-SM mRNA表达;ELISA测定血管内皮细胞细胞因子;同时采用荧光定量PCR和Western blot检测细胞骨架destrin及α-SM蛋白表达。结果酸化环境中HSP患儿血清Ig A1作用血管内皮细胞后,ASIC1a mRNA表达明显上调(P<0.01);细胞因子IL-8、NO和TM分泌明显增加(P<0.01);ASICs阻滞剂可以显著降低酸化诱导的细胞因子的分泌(P<0.01)。细胞外酸化下,HSP患儿血清Ig A1导致细胞骨架蛋白destrin及α-SM mRNA和蛋白表达明显下调(P<0.01);而ASICs阻断剂组destrin及α-SM mRNA和蛋白表达明显上调(P<0.01)。结论ASIC1a在HSP患儿血管炎的发生中起着重要作用,阻断细胞外ASIC1a能显著改善HSP患儿血管内皮细胞损伤。 展开更多
关键词 过敏性紫癜 酸敏感离子通道 血管内皮细胞
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幽门螺杆菌感染胃炎患儿胃黏膜ASICs表达在胃肠道动力中的作用 被引量:5
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作者 王金坤 袁丽萍 +1 位作者 李欢 肖红云 《实用医学杂志》 CAS 北大核心 2021年第6期755-758,共4页
目的探讨幽门螺杆菌(Helicobacter pylori,Hp)感染患儿胃黏膜中酸敏感离子通道(acid-sensing ion channels,ASICs)的表达及其在Hp感染胃炎胃肠功能中的作用。方法将22例经内镜诊断的胃炎患者分为幽门螺杆菌阳性组[Hp(+),n=11]和阴性组[H... 目的探讨幽门螺杆菌(Helicobacter pylori,Hp)感染患儿胃黏膜中酸敏感离子通道(acid-sensing ion channels,ASICs)的表达及其在Hp感染胃炎胃肠功能中的作用。方法将22例经内镜诊断的胃炎患者分为幽门螺杆菌阳性组[Hp(+),n=11]和阴性组[Hp(-),n=11]。免疫化学法检测胃炎患儿胃黏膜中ASICs的表达和定位。ELISA法测定血清CCK、SP、GAS和MTL水平。采用Pearson相关性分析ASIC3表达与胃肠道激素的相关性。结果 Hp感染患者胃黏膜ASIC3表达明显升高,血清CCK、SP、MTL水平明显升高。血清CCK、SP、MTL水平与ASIC3表达密切相关。结论 Hp可诱导胃黏膜ASIC3的表达,其影响Hp感染患者的胃肠激素分泌和胃肠功能。 展开更多
关键词 酸敏感离子通道 幽门螺杆菌感染 胃炎
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Locus Coeruleus Acid-Sensing Ion Channels Modulate Sleep–Wakefulness and State Transition from NREM to REM Sleep in the Rat 被引量:1
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作者 Fayaz A.Mir Sushil K.Jha 《Neuroscience Bulletin》 SCIE CAS CSCD 2021年第5期684-700,共17页
The locus coeruleus(LC) is one of the essential chemoregulatory and sleep–wake(S–W) modulating centers in the brain. LC neurons remain highly active during wakefulness, and some implicitly become silent during rapid... The locus coeruleus(LC) is one of the essential chemoregulatory and sleep–wake(S–W) modulating centers in the brain. LC neurons remain highly active during wakefulness, and some implicitly become silent during rapid eye movement(REM) sleep. LC neurons are also involved in CO_2-dependent modulation of the respiratory drive. Acid-sensing ion channels(ASICs) are highly expressed in some brainstem chemosensory breathing regulatory areas, but their localization and functions in the LC remain unknown. Mild hypercapnia increases the amount of non-REM(NREM) sleep and the number of REM sleep episodes, but whether ASICs in the LC modulate S–W is unclear. Here, we investigated the presence of ASICs in the LC and their role in S–W modulation and the state transition from NREM to REM sleep. Male Wistar rats were surgically prepared for chronic polysomnographic recordings and drug microinjections into the LC. The presence of ASIC-2 and ASIC-3 in the LC was immunohistochemically characterized.Microinjections of amiloride(an ASIC blocker) and APETx2(a blocker of ASIC-2 and-3) into the LC significantly decreased wakefulness and REM sleep, but significantly increased NREM sleep. Mild hypercapnia increased the amount of NREM and the number of REM episodes. However, APETx2 microinjection inhibited this increase in REM frequency. These results suggest that the ASICs of LC neurons modulate S–W, indicating that ASICs could play an important role in vigilance-state transition. A mild increase in CO_2 level during NREM sleep sensed by ASICs could be one of the determinants of state transition from NREM to REM sleep. 展开更多
关键词 acid-sensing ion channels Carbon dioxide HYPERCAPNIA NREM sleep REM sleep
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酸敏感离子通道的功能及其相关调控 被引量:8
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作者 伍龙军 徐天乐 《生物化学与生物物理进展》 SCIE CAS CSCD 北大核心 2003年第3期339-343,共5页
酸敏感离子通道 (ASICs)是一类由胞外酸化所激活的阳离子通道 .目前 ,已发现了 6个ASICs亚基 ,它们在外周和中枢神经系统中广泛表达 .利用基因敲除等技术 ,已证明它们在触觉、痛觉、酸味觉以及学习记忆中具有重要作用 .同时 ,它们也参... 酸敏感离子通道 (ASICs)是一类由胞外酸化所激活的阳离子通道 .目前 ,已发现了 6个ASICs亚基 ,它们在外周和中枢神经系统中广泛表达 .利用基因敲除等技术 ,已证明它们在触觉、痛觉、酸味觉以及学习记忆中具有重要作用 .同时 ,它们也参与某些病理反应 .ASICs可以被神经肽、温度、金属离子和缺血相关物质等调控 。 展开更多
关键词 酸敏感离子通道 功能 相关调控 asics亚基 细胞 信号
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酸敏感离子通道阻断剂阿米洛利对佐剂性关节炎大鼠关节软骨细胞凋亡抑制作用的初步研究 被引量:5
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作者 张腾跃 陈飞虎 +3 位作者 王念 袁凤来 胡伟 吴繁荣 《中国临床药理学与治疗学》 CAS CSCD 2010年第1期41-46,共6页
目的:研究酸敏感离子通道阻断剂阿米洛利(Amiloride)对佐剂性关节炎(AIA)大鼠关节软骨组织的保护作用及机制。方法:将大鼠随机分为正常组、AIA模型组、阿米洛利(2.5、5.0、10.0mg·kg-1.d-1)组和阿司匹林(50mg/kg)对照组。弗氏完全... 目的:研究酸敏感离子通道阻断剂阿米洛利(Amiloride)对佐剂性关节炎(AIA)大鼠关节软骨组织的保护作用及机制。方法:将大鼠随机分为正常组、AIA模型组、阿米洛利(2.5、5.0、10.0mg·kg-1.d-1)组和阿司匹林(50mg/kg)对照组。弗氏完全佐剂(CFA)致AIA后第10天,大鼠出现继发性炎症,此时阿米洛利、阿司匹林灌胃给药,连续8d;正常组与模型组给予等容量的无菌注射用水。检测大鼠继发侧关节肿胀度,光学显微镜观察关节病理变化,透射电镜、Tunel法观察阿米洛利对AIA大鼠关节软骨细胞凋亡的影响,免疫组织化学技术测定阿米洛利对AIA大鼠关节软骨中的Ⅱ型胶原(COII)蛋白合成的影响,Alcian染色测定阿米洛利对AIA大鼠关节软骨中的PG合成的影响。结果:阿米洛利各剂量组对AIA大鼠的足肿胀未见明显影响;病理学观察关节软骨表面光滑,软骨细胞层次尚清晰,成熟软骨细胞数量增加;透射电镜观察软骨细胞核内异染色质轻度边集、细胞内粗面内质网轻度扩张、线粒体轻度肿胀,与模型组软骨细胞核膜皱缩、核染色质高度边集、核膜破裂、出现凋亡小体相比无明显细胞凋亡的特征;Tunel法原位检测显示凋亡阳性细胞数显著减少(P<0.01);免疫组化及Alcian染色检测显示关节软骨基质成分COII蛋白和PG的表达量增加。结论:ASICs阻断剂阿米洛利可通过抑制AIA大鼠关节软骨细胞凋亡发挥对关节软骨的保护作用。 展开更多
关键词 阿米洛利(Amiloride) 佐剂性关节炎(AIA) 酸敏感离子通道(asics) 细胞凋亡
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酸敏感离子通道研究进展 被引量:8
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作者 伍龙军 徐天乐 《生物化学与生物物理进展》 SCIE CAS CSCD 北大核心 2002年第2期197-201,共5页
组织酸化是生理和病理下常见的现象 .神经元可以通过酸敏感的离子通道 (ASICs)来感受细胞周围的pH值的降低 .ASICs属于NaC DEG家族的一个成员 .目前 ,已发现了 6个ASICs亚基 ,它们在外周和中枢神经系统中广泛表达 ,其同聚体和异聚体通... 组织酸化是生理和病理下常见的现象 .神经元可以通过酸敏感的离子通道 (ASICs)来感受细胞周围的pH值的降低 .ASICs属于NaC DEG家族的一个成员 .目前 ,已发现了 6个ASICs亚基 ,它们在外周和中枢神经系统中广泛表达 ,其同聚体和异聚体通道有着各种不同的电生理学特性 .ASICs在机体感觉尤其是痛觉中起着至关重要的作用 . 展开更多
关键词 组织酸化 酸敏感离子通道 痛觉 研究进展
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酸感受性离子通道生物学特性及其调控 被引量:1
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作者 翁谢川 郑建全 +1 位作者 彭双清 李锦 《生物化学与生物物理进展》 SCIE CAS CSCD 北大核心 2007年第1期13-17,共5页
酸感受离子通道(ASICs)为H+-门控的阳离子通道,是一类新的配体门控性离子通道,属于钠通道超家族的新成员.作为近来研究的热点,ASICs具有许多重要的生物学功能,并很有可能成为抗癫痫、镇痛、提高学习记忆能力和保护神经元缺血损伤作用药... 酸感受离子通道(ASICs)为H+-门控的阳离子通道,是一类新的配体门控性离子通道,属于钠通道超家族的新成员.作为近来研究的热点,ASICs具有许多重要的生物学功能,并很有可能成为抗癫痫、镇痛、提高学习记忆能力和保护神经元缺血损伤作用药理学新靶点.近来,ASICs各个亚基已被克隆,它们在生物体内分布、表达、功能和相关调节因素的研究正受到广泛重视. 展开更多
关键词 酸感受离子通道(asics) 钠通道 生物学特性 功能 调控
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酸敏感离子通道在DRG的表达与调控 被引量:1
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作者 张晶 司军强 《农垦医学》 2008年第3期224-226,共3页
目的:酸敏感离子通道(acid sensingion channels,ASICs)为H+-门控的阳离子通道,是一类新的配体门控性离子通道,属于钠通道超家族的新成员。ASICs具有许多重要的生物学功能,在痛觉和触觉调制中具有重要作用。近来,ASICs各个亚基已被克隆... 目的:酸敏感离子通道(acid sensingion channels,ASICs)为H+-门控的阳离子通道,是一类新的配体门控性离子通道,属于钠通道超家族的新成员。ASICs具有许多重要的生物学功能,在痛觉和触觉调制中具有重要作用。近来,ASICs各个亚基已被克隆,并且所有亚基在背根神经节(dorsal root ganglion,DRG)神经元均有表达。本文主要介绍ASICs在DRG的表达与调控。 展开更多
关键词 酸敏感离子通道(asics) 背根神经节(DRG) 调控
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酸敏感离子通道与过敏性紫癜患儿血管内皮损伤关系的探讨
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作者 闫波 房功思 +2 位作者 戴寒晶 孟德娣 袁丽萍 《齐齐哈尔医学院学报》 2017年第12期1365-1368,共4页
目的探讨酸敏感离子通道(ASICs)在过敏性紫癜血管内皮细胞中的表达及在其血管内皮细胞损伤中的作用。方法免疫组化法检测HSP患儿皮肤组织中ASICs各亚型的表达和分布;以人皮肤微血管内皮细胞(HDMECs)为对象,观察不同pH条件下血管内皮细胞... 目的探讨酸敏感离子通道(ASICs)在过敏性紫癜血管内皮细胞中的表达及在其血管内皮细胞损伤中的作用。方法免疫组化法检测HSP患儿皮肤组织中ASICs各亚型的表达和分布;以人皮肤微血管内皮细胞(HDMECs)为对象,观察不同pH条件下血管内皮细胞中ASIC1a、ASIC2a及ASIC3 mRNA的表达,同时观察ASICs阻滞剂对血管内皮细胞IL-8、NO和TM分泌及α-SMA mRNA表达的影响。结果 HSP患儿皮肤血管内皮细胞中ASIC1a及ASIC3的表达显著高于对照组,差异有统计学意义(P<0.05)。随着细胞外酸化,ASIC1a、ASIC2a及ASIC3 mRNA表达明显增加;阻断ASICs,可以显著降低血管内皮细胞中IL-8、NO和TM分泌并促进α-SMA mRNA表达。结论 ASICs可能参与了HSP患儿血管内皮细胞损伤的过程,其机制可能与诱导其进一步发生炎性反应有关。 展开更多
关键词 内皮细胞 紫癜 过敏性 酸敏感离子通道
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酸敏感离子通道的研究进展 被引量:4
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作者 张政 蔡炳祥 关永源 《中国药理学通报》 CAS CSCD 北大核心 2005年第12期1414-1417,共4页
酸敏感通道广泛表达于中枢与外周神经系统,在疼痛、伤害感觉中发挥了重要的作用。其分子结构与分布特点、调控机制以及在某些病理生理过程中的作用已经得到了部分阐明。未来对其研究将是离子通道研究领域的热门课题。
关键词 酸敏感通道 痛觉 伤害感觉 缺血
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酸敏感离子通道在肿瘤中的研究进展 被引量:1
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作者 高森 顾元龙 金成 《临床肿瘤学杂志》 CAS 北大核心 2018年第4期373-378,共6页
近年来研究发现肿瘤细胞微环境中低pH值促进肿瘤生长和转移。酸敏感离子通道(ASICs)作为可被酸性微环境所激活的H^+门控阳离子通道,属于上皮钠离子通道/退化蛋白超家族,被认为参与该过程。事实上,目前已有研究报道,ASICs在神经胶质瘤、... 近年来研究发现肿瘤细胞微环境中低pH值促进肿瘤生长和转移。酸敏感离子通道(ASICs)作为可被酸性微环境所激活的H^+门控阳离子通道,属于上皮钠离子通道/退化蛋白超家族,被认为参与该过程。事实上,目前已有研究报道,ASICs在神经胶质瘤、肝癌、乳腺癌、前列腺癌、胰腺癌、肺癌及肾透明细胞癌等肿瘤的发生、发展中均发挥重要作用。虽然,其具体机制尚未明确,但这些研究结果表明ASICs可能作为一种潜在的新型肿瘤标志物和治疗靶点,为开发肿瘤的替代疗法提供新的思路。本文结合国内外最新研究报道,对ASICs在肿瘤中的研究进展作一综述。 展开更多
关键词 肿瘤 酸敏感离子通道(asics) 酸中毒
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触脑脊液神经元的研究进展 被引量:2
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作者 李瑞 刘海鹰 +4 位作者 王家瑶 郭保霖 高方 武胜昔 王文挺 《神经解剖学杂志》 CAS CSCD 北大核心 2018年第5期633-637,共5页
触脑脊液神经元(cerebrospinal fluid-contacting neurons,CSF-cNs)是一种分布于脑室、中央管、脑室周器及脑实质等处与脑脊液接触的特殊神经元。根据分布位置不同可将CSF-cNs分为室管膜上、室管膜下和远位CSF-cNs三类,不同部位的CSF-... 触脑脊液神经元(cerebrospinal fluid-contacting neurons,CSF-cNs)是一种分布于脑室、中央管、脑室周器及脑实质等处与脑脊液接触的特殊神经元。根据分布位置不同可将CSF-cNs分为室管膜上、室管膜下和远位CSF-cNs三类,不同部位的CSF-cNs分泌不同的神经递质。以往研究CSF-cNs多采用脑室注射辣根过氧化物酶标记的霍乱毒素B亚单位(cholera toxin subunit B labeled with horseradish peroxidase,CB-HRP)进行逆行追踪. 展开更多
关键词 触脑脊液神经元 POLYCYSTIN KIDNEY disease 2-like 1(PKD2L1) acid-sensing ion channels(asic)
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