Background: Juvenile idiopathic arthritis (JIA) is the most common rheumatic disease in children. Both the humoral and cell mediated immunities are involved in the pathogenesis of JIA. It is reported that overall immu...Background: Juvenile idiopathic arthritis (JIA) is the most common rheumatic disease in children. Both the humoral and cell mediated immunities are involved in the pathogenesis of JIA. It is reported that overall immunoglobulin levels in JIA patients are significantly higher than their control during the active state of disease. Methodology: This prospective observational study was conducted over a period of 18 months All the newly diagnosed oligo-articular and poly-articular JIA patients having active disease were included by purposive sampling. Data were collected by a semi-structured predesigned questionnaire. Result: Most of the study subjects (57.6%) belonged to age group > 3 - 9 years. Oligo JIA was diagnosed in 66.7% and poly JIA in 33.3% of JIA children. The difference in mean (±SD) ESR (33.52 ± 21.29 and 15.09 ± 7.71 mm in 1st hour) at active and inactive states was highly significant. Mean (±SD) difference of IgG, IgM and IgA in active and inactive states of disease were highly significant. Conclusion: Higher and abnormal levels of immunoglobulin (IgG, IgM, and IgA) were present among JIA patients in active disease state which became normal during inactive disease state after treatment.展开更多
Objective To produce the antiserum of chicken brain derived neuron growth inhibitor collapsin 1. To detect its ability to neutralize the collapsing activity of collapsin 1. Methods The c myc epitope tagged ent...Objective To produce the antiserum of chicken brain derived neuron growth inhibitor collapsin 1. To detect its ability to neutralize the collapsing activity of collapsin 1. Methods The c myc epitope tagged entire sequence of collapsin 1 was amplified by PCR and expression cloned. Rabbits were immunized with the c myc tagged collapsin 1 to produce antiserum of collapsin 1. Its ability to neutralize the collapsing activity of collapsin 1 was observed in dorsal root ganglia (DRG) growth cone. Results Collapsin 1 could induce the collapse of DRG growth cone. The collapsing activity of collapsin 1 could be neutralized by the antiserum of collapsin 1. Conclusion We produced the antibody of a neuron growth inhibitor collapsin 1 that could block its inhibiting function.展开更多
Drug innovation is characterized by painstaking molecular-level syntheses and modifications as the basic components of research and development.Similarly,natural products are chemically tailored and modified based upo...Drug innovation is characterized by painstaking molecular-level syntheses and modifications as the basic components of research and development.Similarly,natural products are chemically tailored and modified based upon their structural and biological properties.To some extent,the modification of natural products is quite different from de novo structure-based drug discovery.This review describes the general strategies and principles for the modification of natural products to drugs,as illustrated by several successful medicines that originated from natural products.展开更多
文摘Background: Juvenile idiopathic arthritis (JIA) is the most common rheumatic disease in children. Both the humoral and cell mediated immunities are involved in the pathogenesis of JIA. It is reported that overall immunoglobulin levels in JIA patients are significantly higher than their control during the active state of disease. Methodology: This prospective observational study was conducted over a period of 18 months All the newly diagnosed oligo-articular and poly-articular JIA patients having active disease were included by purposive sampling. Data were collected by a semi-structured predesigned questionnaire. Result: Most of the study subjects (57.6%) belonged to age group > 3 - 9 years. Oligo JIA was diagnosed in 66.7% and poly JIA in 33.3% of JIA children. The difference in mean (±SD) ESR (33.52 ± 21.29 and 15.09 ± 7.71 mm in 1st hour) at active and inactive states was highly significant. Mean (±SD) difference of IgG, IgM and IgA in active and inactive states of disease were highly significant. Conclusion: Higher and abnormal levels of immunoglobulin (IgG, IgM, and IgA) were present among JIA patients in active disease state which became normal during inactive disease state after treatment.
基金Thisprojectissupportedbythenaturalsciencefundofthenation (No 39840 0 11)andthatofJiangsuprovince (BK970 5 7)
文摘Objective To produce the antiserum of chicken brain derived neuron growth inhibitor collapsin 1. To detect its ability to neutralize the collapsing activity of collapsin 1. Methods The c myc epitope tagged entire sequence of collapsin 1 was amplified by PCR and expression cloned. Rabbits were immunized with the c myc tagged collapsin 1 to produce antiserum of collapsin 1. Its ability to neutralize the collapsing activity of collapsin 1 was observed in dorsal root ganglia (DRG) growth cone. Results Collapsin 1 could induce the collapse of DRG growth cone. The collapsing activity of collapsin 1 could be neutralized by the antiserum of collapsin 1. Conclusion We produced the antibody of a neuron growth inhibitor collapsin 1 that could block its inhibiting function.
文摘Drug innovation is characterized by painstaking molecular-level syntheses and modifications as the basic components of research and development.Similarly,natural products are chemically tailored and modified based upon their structural and biological properties.To some extent,the modification of natural products is quite different from de novo structure-based drug discovery.This review describes the general strategies and principles for the modification of natural products to drugs,as illustrated by several successful medicines that originated from natural products.
