Assays that measure steroid hormones in patient care, public health, and research need to be both accurate and precise, as these criteria help to ensure comparability across all clinical and research applications. Thi...Assays that measure steroid hormones in patient care, public health, and research need to be both accurate and precise, as these criteria help to ensure comparability across all clinical and research applications. This review addresses major issues relevant to assay variability and describes recent activities by the US Centers for Disease Control and Prevention （CDC） to improve assay performance. Currently, high degrees of accuracy and precision are not always met for testosterone and estradiol measurements; although technologies for steroid hormone measurement have advanced significantly, measurement variability within and across laboratories has not improved accordingly. Differences in calibration and specificity are discussed as sources of variability in measurement accuracy. Ultimately, a combination of factors appears to cause inaccuracy of steroid hormone measurements, with nonuniform assay calibration and lack of specificity being two major contributors to assay variability. Within-assay variability for current assays is generally high, especially at low analyte concentrations. The CDC Hormone Standardization （HoSt） Program is improving clinical assays, as evidenced by a 50% decline in mean absolute bias between mass spectrometry assays and the CDC reference method from 2007 to 2011. This program provides the measurement traceability to CDC reference methods and helps to minimize factors affecting measurement variability.展开更多
In the present paper, changes of plasma testosterone and estradiol contents after moxibustion were analyzed in the old subjects. Results show that plasma testosterone of the old male subjects and estradiol content of ...In the present paper, changes of plasma testosterone and estradiol contents after moxibustion were analyzed in the old subjects. Results show that plasma testosterone of the old male subjects and estradiol content of the old male and female subjects rise following moxibustion. It indicatesthat moxibustion can regulate the functional state of the hypothalam us-hypophysis- sexual glang axis,improve neuroendocrine disorder, prevent the sexual glang from atrophy and delay senility. Increase ofthe two hormones in the human body can lead to a series of regulations in other respects. Therefore,moxibustion is of an important role in delaying senility.展开更多
The imminent danger of the Covid-19 pandemic has accelerated research in pharmaceuticals that either target the viral Spike proteins fusion with ACE2 receptors,or the infectious RNA replication that often overwhelms i...The imminent danger of the Covid-19 pandemic has accelerated research in pharmaceuticals that either target the viral Spike proteins fusion with ACE2 receptors,or the infectious RNA replication that often overwhelms immune defences.The scope of this review was to elucidate the main human vulnerabilities to Covid-19,including the accumulation of ACE2 receptors in testes,adipose tissue,thyroid,heart and kidneys that escalate viral affinity in males,the aged,and certain medical conditions,including diabetes,CVD,and pulmonary diseases.Pre-existing inflammation inherent in obesity may exacerbate the“cytokine storm,”a rampaging immune reaction during the course of Covid-19 that is deleterious to the host.We examined the molecular dynamics illustrating the action of new therapeutics necessary for Covid-19 patients;the estradiol advantage hypothesis;alternative therapies including hormone replacement procedures and mesenchymal stem cells;plus preventive and protective interventions.The current perspective also explored the primary components of dysregulated health predisposing individuals to Covid-19,including hormonal imbalance,increased lipids and lipoproteins,thyroid dysfunction,degraded fitness,and age-related testosterone decline accompanied by cortisol increase that provokes stress eating behaviours and weight accumulation.Obesity increases the probability of Covid-19 infection due to its abundance of ACE2 receptors;while physical activity may decrease Covid-19 vulnerability,by reducing fat and increasing muscle mass that manifests a relatively inhibited ACE2 expression.Several weight management solutions feature lasers and radiofrequency which diminish subcutaneous adiposity but do not enhance fitness.A data metanalysis of seven recently published clinical studies on 95 obese individuals,73 males and 22 females with an average BMI of 30.9,demonstrated visceral fat reduction combined with increased skeletal muscle mass.It also revealed a statistically significant decrease in BMI,lipids,lipoproteins,inflammation and toxicity as measured by CRP,Creatinine and Bilirubin respectively,juxtaposed by optimally healthier levels of Cortisol,Testosterone,Free T3,IGF-1,Insulin,and the appetite controlling hormones Leptin and Ghrelin.展开更多
Transmasculine individuals,considering whether to undergo total hysterectomy with bilateral salpingectomy,have the option to have a concomitant oophorectomy.While studies have evaluated hormone changes following testo...Transmasculine individuals,considering whether to undergo total hysterectomy with bilateral salpingectomy,have the option to have a concomitant oophorectomy.While studies have evaluated hormone changes following testosterone therapy initiation,most of those patients have not undergone oophorectomy.Data are currently limited to support health outcomes regarding the decision to retain or remove the ovaries.We performed a retrospective chart review of transmasculine patients maintained on high-dose testosterone therapy at a single endocrine clinic in Vancouver,British Columbia,Canada.Twelve transmasculine individuals who underwent bilateral oophorectomy and had presurgical and postsurgical serum data were included.We identified 12 transmasculine subjects as controls,who were on testosterone therapy and did not undergo oophorectomy,but additionally matched to the first group by age,testosterone dosing regimen,and body mass index.There was a statistically significant decrease in the estradiol levels of case subjects postoophorectomy,when compared to presurgical estradiol levels(P=0.02).There was no significant difference between baseline estradiol levels between control and case subjects;however,the difference in estradiol levels at follow-up measurements was significant(P=0.03).Total testosterone levels did not differ between control and case subjects at baseline and follow-up(both P>0.05).Our results demonstrate that oophorectomy further attenuates estradiol levels below what is achieved by high-dose exogenous testosterone alone.Correlated clinical outcomes,such as impacts on bone health,were not available.The clinical implications of oophorectomy versus ovarian retention on endocrinological and overall health outcomes are currently limited.展开更多
Objective To evaluate the serum concentrations of estradiol and testosterone in hyperuricemia patients and possible correlations of the two factors and uric acid(UA)in hyperuricemia(HUA)patients.Methods This was a cas...Objective To evaluate the serum concentrations of estradiol and testosterone in hyperuricemia patients and possible correlations of the two factors and uric acid(UA)in hyperuricemia(HUA)patients.Methods This was a case control study,which involved 90 hyperuricemia patients,103 healthy controls.Estradiol,testosterone,UA。展开更多
Erectile dysfunctio n attributable to testosterone deficiency is less comm on in young males,and the effect of estradiol on erectile function in eug on adal young males is un clear.We an a lyzed data from 195 male par...Erectile dysfunctio n attributable to testosterone deficiency is less comm on in young males,and the effect of estradiol on erectile function in eug on adal young males is un clear.We an a lyzed data from 195 male participa nts,including 143 eug on adal patients with erectile dysfunction and 52 healthy men.To distinguish psychogenic and organic erectile dysfunction,penile rigidity was measured using the nocturnal penile tumescenee rigidity test.Serum levels of sexual hormones were quantified by electrochemiluminescence,and penile vascular status was assessed by penile color Doppler ultrasound.Both serum estradiol levels and the ratio of estradiol to testosterone were higher in patients with organic erectile dysfunction than in patients with psychogenic erectile dysfunction or healthy controls.Organic erectile dysfunction was negatively associated with estradiol levels and the ratio of estradiol to testosterone,and estradiol was the only sign ifica nt risk factor for orga nic erectile dysf un ction(odds ratio:1.094;95%con fide nee interval:1.042-1.149,P=0.000).Moreover,serum estradiol levels were negatively correlated with penile rigidity.Serum estradiol levels were higher and penile rigidity was lower in patients with venous erectile dysfunction than in patients with nonvascular erectile dysf unction.We con elude that elevated serum estradiol levels may impair erectile function and may be involved in the pathoge nesis of organic erectile dysfunction in eugonadal young men.展开更多
文摘Assays that measure steroid hormones in patient care, public health, and research need to be both accurate and precise, as these criteria help to ensure comparability across all clinical and research applications. This review addresses major issues relevant to assay variability and describes recent activities by the US Centers for Disease Control and Prevention （CDC） to improve assay performance. Currently, high degrees of accuracy and precision are not always met for testosterone and estradiol measurements; although technologies for steroid hormone measurement have advanced significantly, measurement variability within and across laboratories has not improved accordingly. Differences in calibration and specificity are discussed as sources of variability in measurement accuracy. Ultimately, a combination of factors appears to cause inaccuracy of steroid hormone measurements, with nonuniform assay calibration and lack of specificity being two major contributors to assay variability. Within-assay variability for current assays is generally high, especially at low analyte concentrations. The CDC Hormone Standardization （HoSt） Program is improving clinical assays, as evidenced by a 50% decline in mean absolute bias between mass spectrometry assays and the CDC reference method from 2007 to 2011. This program provides the measurement traceability to CDC reference methods and helps to minimize factors affecting measurement variability.
文摘In the present paper, changes of plasma testosterone and estradiol contents after moxibustion were analyzed in the old subjects. Results show that plasma testosterone of the old male subjects and estradiol content of the old male and female subjects rise following moxibustion. It indicatesthat moxibustion can regulate the functional state of the hypothalam us-hypophysis- sexual glang axis,improve neuroendocrine disorder, prevent the sexual glang from atrophy and delay senility. Increase ofthe two hormones in the human body can lead to a series of regulations in other respects. Therefore,moxibustion is of an important role in delaying senility.
文摘The imminent danger of the Covid-19 pandemic has accelerated research in pharmaceuticals that either target the viral Spike proteins fusion with ACE2 receptors,or the infectious RNA replication that often overwhelms immune defences.The scope of this review was to elucidate the main human vulnerabilities to Covid-19,including the accumulation of ACE2 receptors in testes,adipose tissue,thyroid,heart and kidneys that escalate viral affinity in males,the aged,and certain medical conditions,including diabetes,CVD,and pulmonary diseases.Pre-existing inflammation inherent in obesity may exacerbate the“cytokine storm,”a rampaging immune reaction during the course of Covid-19 that is deleterious to the host.We examined the molecular dynamics illustrating the action of new therapeutics necessary for Covid-19 patients;the estradiol advantage hypothesis;alternative therapies including hormone replacement procedures and mesenchymal stem cells;plus preventive and protective interventions.The current perspective also explored the primary components of dysregulated health predisposing individuals to Covid-19,including hormonal imbalance,increased lipids and lipoproteins,thyroid dysfunction,degraded fitness,and age-related testosterone decline accompanied by cortisol increase that provokes stress eating behaviours and weight accumulation.Obesity increases the probability of Covid-19 infection due to its abundance of ACE2 receptors;while physical activity may decrease Covid-19 vulnerability,by reducing fat and increasing muscle mass that manifests a relatively inhibited ACE2 expression.Several weight management solutions feature lasers and radiofrequency which diminish subcutaneous adiposity but do not enhance fitness.A data metanalysis of seven recently published clinical studies on 95 obese individuals,73 males and 22 females with an average BMI of 30.9,demonstrated visceral fat reduction combined with increased skeletal muscle mass.It also revealed a statistically significant decrease in BMI,lipids,lipoproteins,inflammation and toxicity as measured by CRP,Creatinine and Bilirubin respectively,juxtaposed by optimally healthier levels of Cortisol,Testosterone,Free T3,IGF-1,Insulin,and the appetite controlling hormones Leptin and Ghrelin.
文摘Transmasculine individuals,considering whether to undergo total hysterectomy with bilateral salpingectomy,have the option to have a concomitant oophorectomy.While studies have evaluated hormone changes following testosterone therapy initiation,most of those patients have not undergone oophorectomy.Data are currently limited to support health outcomes regarding the decision to retain or remove the ovaries.We performed a retrospective chart review of transmasculine patients maintained on high-dose testosterone therapy at a single endocrine clinic in Vancouver,British Columbia,Canada.Twelve transmasculine individuals who underwent bilateral oophorectomy and had presurgical and postsurgical serum data were included.We identified 12 transmasculine subjects as controls,who were on testosterone therapy and did not undergo oophorectomy,but additionally matched to the first group by age,testosterone dosing regimen,and body mass index.There was a statistically significant decrease in the estradiol levels of case subjects postoophorectomy,when compared to presurgical estradiol levels(P=0.02).There was no significant difference between baseline estradiol levels between control and case subjects;however,the difference in estradiol levels at follow-up measurements was significant(P=0.03).Total testosterone levels did not differ between control and case subjects at baseline and follow-up(both P>0.05).Our results demonstrate that oophorectomy further attenuates estradiol levels below what is achieved by high-dose exogenous testosterone alone.Correlated clinical outcomes,such as impacts on bone health,were not available.The clinical implications of oophorectomy versus ovarian retention on endocrinological and overall health outcomes are currently limited.
文摘Objective To evaluate the serum concentrations of estradiol and testosterone in hyperuricemia patients and possible correlations of the two factors and uric acid(UA)in hyperuricemia(HUA)patients.Methods This was a case control study,which involved 90 hyperuricemia patients,103 healthy controls.Estradiol,testosterone,UA。
基金This work was supported by grants from the National Key Research and Development Program(No.2017YFC1002003)the National Science Foundation for Young Scientists of China(No.81701428).
文摘Erectile dysfunctio n attributable to testosterone deficiency is less comm on in young males,and the effect of estradiol on erectile function in eug on adal young males is un clear.We an a lyzed data from 195 male participa nts,including 143 eug on adal patients with erectile dysfunction and 52 healthy men.To distinguish psychogenic and organic erectile dysfunction,penile rigidity was measured using the nocturnal penile tumescenee rigidity test.Serum levels of sexual hormones were quantified by electrochemiluminescence,and penile vascular status was assessed by penile color Doppler ultrasound.Both serum estradiol levels and the ratio of estradiol to testosterone were higher in patients with organic erectile dysfunction than in patients with psychogenic erectile dysfunction or healthy controls.Organic erectile dysfunction was negatively associated with estradiol levels and the ratio of estradiol to testosterone,and estradiol was the only sign ifica nt risk factor for orga nic erectile dysf un ction(odds ratio:1.094;95%con fide nee interval:1.042-1.149,P=0.000).Moreover,serum estradiol levels were negatively correlated with penile rigidity.Serum estradiol levels were higher and penile rigidity was lower in patients with venous erectile dysfunction than in patients with nonvascular erectile dysf unction.We con elude that elevated serum estradiol levels may impair erectile function and may be involved in the pathoge nesis of organic erectile dysfunction in eugonadal young men.
