This work;It was made to determine the positive and negative usage information, attitudes and behaviors of social networking networks for users. Today, it is well known how the wide social effects of social networking...This work;It was made to determine the positive and negative usage information, attitudes and behaviors of social networking networks for users. Today, it is well known how the wide social effects of social networking sites are widespread and effective. As far as it can be followed from written and visual media, it causes significant damages for attitudes and behaviors for individuals. Looking at the results of the study, it seems impossible to limit the time and necessity of these networks for people, but it is understood that people force their limits and capacity. It was determined that the general acceptance and rejection areas are mixed and the dominant distinctive effects were weakened. Unable to prevent social dissolution and deterioration was realized by these social networks of networks. However, we cannot only talk about negative effects because their positive effects should be mentioned. Data were obtained in this direction. For example, some segments encourage the users of these sites to have more freedom, to develop democracy and human rights awareness. However, the real problem is when and where to stand. This study;Social networking networks use information, attitudes and behaviors;It was carried out for the purpose of evaluating the use of nicknames and networks for the use of networks. The data of the study were obtained by the application of a questionnaire and the data obtained were analyzed with appropriate statistical analysis methods. In the first part of the study, field study/operational concept scanning is performed and theoretical information is included. In the second part, the purpose of the study, the problem status, the importance of the study, limitations, assumptions, problem question, research method, research universe and sample are specified and informed. In the third section, the data obtained using appropriate statistical methods are analyzed. In the last section, some important suggestions and comments are made in the light of the data obtained.展开更多
Soil pot experiments were conducted in a greenhouse to examine the effects of different nitrogen (N) supply (low, 0.15 g N/kg; middle, 0.3 g N/kg; and high, 0.6 g N/kg dry soil) on the growth, photosynthetic chara...Soil pot experiments were conducted in a greenhouse to examine the effects of different nitrogen (N) supply (low, 0.15 g N/kg; middle, 0.3 g N/kg; and high, 0.6 g N/kg dry soil) on the growth, photosynthetic characteristics and photosynthetic nitrogen use efficiency (PNUE) of tobacco seedlings (Nicotiana tabacum L. Yunyan 87). The results showed middle and high N significantly enhanced seedling growth including plant stem and leaf dry weight comparing with low N. High N supply could lead to a dramatic increase in the photosynthetic capacity of tobacco seedlings under low N conditions. There were significant differences in leaf N content between nitrogen treatments. About a 76% increase in leafN content in plants fed by high N resulted in about 43% increase in Rubisco content and 27% in net photosynthetic rate. The non-corresponding increases in photosynthetic rate in tobacco seedlings fed by high N relative to low N resulted from Rubisco activity and/or carboxylation efficiency (CE). These results indicated that tobacco seedlings under high N application can maintain high net photosynthetic rate (Pn) but lower PNUE, will finally result in a decline in N use efficiency.展开更多
[Objective] This study was conducted to screen a synergistic biological fungicide complex to control Fusarium wilt, reducing the use of chemical pesticides. [Method] The inhibitory effects of Bacil us subtilis DJ-6 an...[Objective] This study was conducted to screen a synergistic biological fungicide complex to control Fusarium wilt, reducing the use of chemical pesticides. [Method] The inhibitory effects of Bacil us subtilis DJ-6 and pyraclostrobin alone or in combination at five ratios against Fusarium oxysporum were detected by mea-suring mycelium growth rate in laboratory tests. The growth promotion and disease control effect of combined or single use of 20% pyraclostrobin and 2 ×1011 cfu/g B. subtilis DJ-6 WP at 1∶1 000, 1∶2 000 and 1∶3 000 dilutions were detected in field trials. [Result] The EC50 values of combined use of B. subtilis DJ-6 and pyra-clostrobin at ratios of 1∶1, 1∶2, 1∶3, 1∶4 and 1∶5 against F. oxysporum were 5.311 5, 4.008 6, 3.570 6, 3.350 9 and 3.218 9 μg/ml, with the synergistic ratios (SR) of 2.28, 1.77, 1.53, 1.64, 1.11, among which the synergetic effect at 1∶1 was the best. The fungicidal activity of pyraclostrobin was greater than that of B. subtilis DJ-6 in laboratory tests. Field trials revealed that al the 1∶1 000, 1∶2 000 and 1∶3 000 dilu-tions of 20% pyraclostrobin·2×1011 cfu/g B. subtilis DJ-6 WP in combination, 1∶1 000 dilution of 1 ×1012 cfu/g B. subtilis DJ-6 WP and 1∶2 000 dilution of 250 g/L pyra-clostrobin EC promoted the growth of strawberry by increasing plant height, leaf petiole, leaf blade area and stem diameter. Among them, the treatments with 1∶1 000 and 1∶2 000 of 20% pyraclostrobin · 2×1011 cfu/g B. subtilis DJ-6 WP in combina-tion had better effects than other treatments. The control effects of al the treat-ments were measured 30 and 80 d after fungicide application. The control effects of 1∶1 000 dilution of 20% pyraclostrobin and 2×1011 cfu/g B. subtilis DJ-6 in combina-tion were up to 100% and 93.11%, which were higher than those in al other treat-ments. The second highest control effects were found in the treatment with 1∶ 2 000 dilution of 20% pyraclostrobin and 2×1011 cfu/g B. subtilis DJ-6 in combination, they were 92.49% and 86.49%, higher than those in other treatments except the 1∶1 000 dilution of 20% pyraclostrobin and 2 ×1011 cfu/g B. subtilis DJ-6 in combination. The control effects of 1∶3 000 dilution of 20% pyraclostrobin and 2×1011 cfu/g B. subtilis DJ-6 in combination were 82.61% and 72.42%, higher than those in treatment with 1∶1 000 dilution of 1×1012 cfu/g B. subtilis DJ-6 WP, but lower than those in treat-ment with 1∶2 000 dilution of 25% pyraclostrobin EC. [Conclusion] Al the results re-vealed that the combination use of 20% pyraclostrobin and 2 ×1011 cfu/g B. subtilis DJ-6 WP at 1∶1 000 to 1∶2 000 dilution had better control effect against strawberry Fusarium wilt.展开更多
Urban recreationists consist of those who have used urban recreational facilities, no matter they are local residents or nonlocals. Traveling models will exert impacts not only on the use of urban recreational facilit...Urban recreationists consist of those who have used urban recreational facilities, no matter they are local residents or nonlocals. Traveling models will exert impacts not only on the use of urban recreational facilities but also on the development and management of recreational resources. Taking Shanghai as a sample of destinaton and origin of urban recreationists, the author did his study based on data from comprehensive travel measurement and users' survey, including field questionnaire survey, mailing questionnaire survey, home survey, and cordon monitonng. To analyze traveing models of urban recreationists in Shanghai, the author used techniques of Recreational Activity Space (RAS) and the Use Curve (UC) Analysis. Rogarding Shanghai as an origin, the author first discussed types and characteristics of the RASs which are presented by isotourist lines in the proper and outskirts of Shanghai, the Yangtze Delta and the China's Mainland respectively. These RASs reflect the trave directional bias of Shanghainese. Then the author regarded Shanghai as a deshnahon and analyzed the RASs of tounsts from Otier provinces, which demonstrated the attractiveness of and distance decay of Shanghai. He found that a U-shaped UC model existed in the distance decay under the influence of attractiveness and distance. Finally, the author suggested, that the availability, periodicity and attractiveness, besides distance, are the basic mechanisms that affect the urban recreationists' traveling models in a city such as Shanghai.展开更多
Waxy maize is a specialty maize that produces mainly amylopectin starch with special food or industrial values. The objective of this study was to overcome the limitations of wx mutant allele acquisition and breeding ...Waxy maize is a specialty maize that produces mainly amylopectin starch with special food or industrial values. The objective of this study was to overcome the limitations of wx mutant allele acquisition and breeding efficiency by conversion of parental lines from normal to waxy maize. The intended mutation activity was achieved by in vivo CRISPR/Cas9 machinery involving desired-target mutation of the Wx locus in the ZC01 background,abbreviated as ZC01-DTM^(wx). Triple selection was applied to segregants to obtain high genome background recovery with transgene-free wx mutations. The targeted mutation was identified, yielding six types of mutations among progeny crossed with ZC01-DTM^(wx).The amylopectin contents of the endosperm starch in mutant lines and hybrids averaged94.9%, while those of the wild-type controls were significantly(P < 0.01) lower, with an average of 76.9%. Double selection in transgene-free lines was applied using the Bar strip test and Cas9 PCR screening. The genome background recovery ratios of the lines were determined using genome-wide SNP data. That of lines used as male parents was as high as98.19% and that of lines used as female parents was as high as 86.78%. Conversion hybrids and both parental lines showed agronomic performance similar to that of their wild-type counterparts. This study provides a practical example of the efficient extension of CRISPR/Cas9 targeted mutation to industrial hybrids for transformation of a recalcitrant species.展开更多
The solution to the state response of active constrained layer and the expression of the sound field radiated from the vibrating structure are given, and the damping mechanisms of active constrained layer (ACL) are di...The solution to the state response of active constrained layer and the expression of the sound field radiated from the vibrating structure are given, and the damping mechanisms of active constrained layer (ACL) are discussed. Compaxisons are made with the passive constrained layer (PCL) and the traditional active control method in reducing the structural vibration. The numerical results indicate that: using the active constrained layer damping (ACLD) treatment to dissipate energyt not only the vibration amplitude of structtire is reduced, but also the sound radiated from vibrating structure is suppressed展开更多
Chagas disease is caused by the protozoan parasite Trypanosoma cruzi.This disease is also known as American trypanosomiasis and approximately 7-8 million people are currently infected(WHO,2014.Nifurtimox has been used...Chagas disease is caused by the protozoan parasite Trypanosoma cruzi.This disease is also known as American trypanosomiasis and approximately 7-8 million people are currently infected(WHO,2014.Nifurtimox has been used for over 40 years to treat Chagas disease,however,this drug is only effective during the acute phase of infection,and certain protozoan strains have developed resistance展开更多
The role of the rhizosphere microbiome remain crucial to human existence and can therefore not be over-looked,particularly with respect to plant growth,development and yield.The rhizosphere is the'home'for sev...The role of the rhizosphere microbiome remain crucial to human existence and can therefore not be over-looked,particularly with respect to plant growth,development and yield.The rhizosphere is the'home'for several genes,some of which are responsible for the various trait and features exhibited by plants.Numerous microbes have been identified to inhabit the rhizosphere,some of which are the proteobacteria,nitrogen fixing bacteria,plant growth promoting rhizobacteria,mycorrhizal and myco-parasitic fungi.Likewise,chemical compounds such as amino acids(crystathionine and homoserine),which inhibits nematodes,carbohydrates(fructose),which enhance the growth of microorganisms,and sterols(sitosterol),which regulate plant growth,reside in the plant rhizosphere.Several factors con-tributes to the existence and survival of these microbes,one being good agricultural practices,as some farming techniques negatively affects the soil microbial content.This study therefore focused on the agricultural and health importance of the soil organisms,as well as the various techniques that can be employed to maintain the rhizosphere microbiome,despite the rise and intensification of various harmful land use activities.This will enable a better understanding of the soil rhizosphere conservation,restoration and sustainable use,specifically of degraded land.This will avoid depleting or alternating the soil microbial content while aiming to ensure a healthy ecosystem,biodiversity,maximal crop produc-tivity,food security and all round sustainability,as targeted by the United Nation's Sustainable De-velopments Goals.展开更多
文摘This work;It was made to determine the positive and negative usage information, attitudes and behaviors of social networking networks for users. Today, it is well known how the wide social effects of social networking sites are widespread and effective. As far as it can be followed from written and visual media, it causes significant damages for attitudes and behaviors for individuals. Looking at the results of the study, it seems impossible to limit the time and necessity of these networks for people, but it is understood that people force their limits and capacity. It was determined that the general acceptance and rejection areas are mixed and the dominant distinctive effects were weakened. Unable to prevent social dissolution and deterioration was realized by these social networks of networks. However, we cannot only talk about negative effects because their positive effects should be mentioned. Data were obtained in this direction. For example, some segments encourage the users of these sites to have more freedom, to develop democracy and human rights awareness. However, the real problem is when and where to stand. This study;Social networking networks use information, attitudes and behaviors;It was carried out for the purpose of evaluating the use of nicknames and networks for the use of networks. The data of the study were obtained by the application of a questionnaire and the data obtained were analyzed with appropriate statistical analysis methods. In the first part of the study, field study/operational concept scanning is performed and theoretical information is included. In the second part, the purpose of the study, the problem status, the importance of the study, limitations, assumptions, problem question, research method, research universe and sample are specified and informed. In the third section, the data obtained using appropriate statistical methods are analyzed. In the last section, some important suggestions and comments are made in the light of the data obtained.
文摘Soil pot experiments were conducted in a greenhouse to examine the effects of different nitrogen (N) supply (low, 0.15 g N/kg; middle, 0.3 g N/kg; and high, 0.6 g N/kg dry soil) on the growth, photosynthetic characteristics and photosynthetic nitrogen use efficiency (PNUE) of tobacco seedlings (Nicotiana tabacum L. Yunyan 87). The results showed middle and high N significantly enhanced seedling growth including plant stem and leaf dry weight comparing with low N. High N supply could lead to a dramatic increase in the photosynthetic capacity of tobacco seedlings under low N conditions. There were significant differences in leaf N content between nitrogen treatments. About a 76% increase in leafN content in plants fed by high N resulted in about 43% increase in Rubisco content and 27% in net photosynthetic rate. The non-corresponding increases in photosynthetic rate in tobacco seedlings fed by high N relative to low N resulted from Rubisco activity and/or carboxylation efficiency (CE). These results indicated that tobacco seedlings under high N application can maintain high net photosynthetic rate (Pn) but lower PNUE, will finally result in a decline in N use efficiency.
基金Supported by Agricultural Science and Technology Research and Development Program of Jiangsu Province(BE2012378)Six Talent Peaks Project of Jiangsu Province(2013-NY-001)Agricultural Science and Technology Research and Development Program of Zhenjiang City(NY2014029)~~
文摘[Objective] This study was conducted to screen a synergistic biological fungicide complex to control Fusarium wilt, reducing the use of chemical pesticides. [Method] The inhibitory effects of Bacil us subtilis DJ-6 and pyraclostrobin alone or in combination at five ratios against Fusarium oxysporum were detected by mea-suring mycelium growth rate in laboratory tests. The growth promotion and disease control effect of combined or single use of 20% pyraclostrobin and 2 ×1011 cfu/g B. subtilis DJ-6 WP at 1∶1 000, 1∶2 000 and 1∶3 000 dilutions were detected in field trials. [Result] The EC50 values of combined use of B. subtilis DJ-6 and pyra-clostrobin at ratios of 1∶1, 1∶2, 1∶3, 1∶4 and 1∶5 against F. oxysporum were 5.311 5, 4.008 6, 3.570 6, 3.350 9 and 3.218 9 μg/ml, with the synergistic ratios (SR) of 2.28, 1.77, 1.53, 1.64, 1.11, among which the synergetic effect at 1∶1 was the best. The fungicidal activity of pyraclostrobin was greater than that of B. subtilis DJ-6 in laboratory tests. Field trials revealed that al the 1∶1 000, 1∶2 000 and 1∶3 000 dilu-tions of 20% pyraclostrobin·2×1011 cfu/g B. subtilis DJ-6 WP in combination, 1∶1 000 dilution of 1 ×1012 cfu/g B. subtilis DJ-6 WP and 1∶2 000 dilution of 250 g/L pyra-clostrobin EC promoted the growth of strawberry by increasing plant height, leaf petiole, leaf blade area and stem diameter. Among them, the treatments with 1∶1 000 and 1∶2 000 of 20% pyraclostrobin · 2×1011 cfu/g B. subtilis DJ-6 WP in combina-tion had better effects than other treatments. The control effects of al the treat-ments were measured 30 and 80 d after fungicide application. The control effects of 1∶1 000 dilution of 20% pyraclostrobin and 2×1011 cfu/g B. subtilis DJ-6 in combina-tion were up to 100% and 93.11%, which were higher than those in al other treat-ments. The second highest control effects were found in the treatment with 1∶ 2 000 dilution of 20% pyraclostrobin and 2×1011 cfu/g B. subtilis DJ-6 in combination, they were 92.49% and 86.49%, higher than those in other treatments except the 1∶1 000 dilution of 20% pyraclostrobin and 2 ×1011 cfu/g B. subtilis DJ-6 in combination. The control effects of 1∶3 000 dilution of 20% pyraclostrobin and 2×1011 cfu/g B. subtilis DJ-6 in combination were 82.61% and 72.42%, higher than those in treatment with 1∶1 000 dilution of 1×1012 cfu/g B. subtilis DJ-6 WP, but lower than those in treat-ment with 1∶2 000 dilution of 25% pyraclostrobin EC. [Conclusion] Al the results re-vealed that the combination use of 20% pyraclostrobin and 2 ×1011 cfu/g B. subtilis DJ-6 WP at 1∶1 000 to 1∶2 000 dilution had better control effect against strawberry Fusarium wilt.
文摘Urban recreationists consist of those who have used urban recreational facilities, no matter they are local residents or nonlocals. Traveling models will exert impacts not only on the use of urban recreational facilities but also on the development and management of recreational resources. Taking Shanghai as a sample of destinaton and origin of urban recreationists, the author did his study based on data from comprehensive travel measurement and users' survey, including field questionnaire survey, mailing questionnaire survey, home survey, and cordon monitonng. To analyze traveing models of urban recreationists in Shanghai, the author used techniques of Recreational Activity Space (RAS) and the Use Curve (UC) Analysis. Rogarding Shanghai as an origin, the author first discussed types and characteristics of the RASs which are presented by isotourist lines in the proper and outskirts of Shanghai, the Yangtze Delta and the China's Mainland respectively. These RASs reflect the trave directional bias of Shanghainese. Then the author regarded Shanghai as a deshnahon and analyzed the RASs of tounsts from Otier provinces, which demonstrated the attractiveness of and distance decay of Shanghai. He found that a U-shaped UC model existed in the distance decay under the influence of attractiveness and distance. Finally, the author suggested, that the availability, periodicity and attractiveness, besides distance, are the basic mechanisms that affect the urban recreationists' traveling models in a city such as Shanghai.
基金supported the National Transgenic Science and Technology Program(2019ZX08010-003)the National Natural Science Foundation of China(31771808)+3 种基金the National Key Research and Development Program of China(2016YFD0101803)the Key Area Research and Development Program of Guangdong Province(2018B020202008)Beijing Municipal Science and Technology Commission(D171100007717001)National Engineering Laboratory for Crop Molecular Breeding。
文摘Waxy maize is a specialty maize that produces mainly amylopectin starch with special food or industrial values. The objective of this study was to overcome the limitations of wx mutant allele acquisition and breeding efficiency by conversion of parental lines from normal to waxy maize. The intended mutation activity was achieved by in vivo CRISPR/Cas9 machinery involving desired-target mutation of the Wx locus in the ZC01 background,abbreviated as ZC01-DTM^(wx). Triple selection was applied to segregants to obtain high genome background recovery with transgene-free wx mutations. The targeted mutation was identified, yielding six types of mutations among progeny crossed with ZC01-DTM^(wx).The amylopectin contents of the endosperm starch in mutant lines and hybrids averaged94.9%, while those of the wild-type controls were significantly(P < 0.01) lower, with an average of 76.9%. Double selection in transgene-free lines was applied using the Bar strip test and Cas9 PCR screening. The genome background recovery ratios of the lines were determined using genome-wide SNP data. That of lines used as male parents was as high as98.19% and that of lines used as female parents was as high as 86.78%. Conversion hybrids and both parental lines showed agronomic performance similar to that of their wild-type counterparts. This study provides a practical example of the efficient extension of CRISPR/Cas9 targeted mutation to industrial hybrids for transformation of a recalcitrant species.
文摘The solution to the state response of active constrained layer and the expression of the sound field radiated from the vibrating structure are given, and the damping mechanisms of active constrained layer (ACL) are discussed. Compaxisons are made with the passive constrained layer (PCL) and the traditional active control method in reducing the structural vibration. The numerical results indicate that: using the active constrained layer damping (ACLD) treatment to dissipate energyt not only the vibration amplitude of structtire is reduced, but also the sound radiated from vibrating structure is suppressed
基金the Universidad Autonoma de Nuevo León(Mexico)for PAICYT grants CN-422-10 and CN-662-11
文摘Chagas disease is caused by the protozoan parasite Trypanosoma cruzi.This disease is also known as American trypanosomiasis and approximately 7-8 million people are currently infected(WHO,2014.Nifurtimox has been used for over 40 years to treat Chagas disease,however,this drug is only effective during the acute phase of infection,and certain protozoan strains have developed resistance
基金the National Research Foundation for grant(ref:UID123634)which supports several research activities in her laboratory.
文摘The role of the rhizosphere microbiome remain crucial to human existence and can therefore not be over-looked,particularly with respect to plant growth,development and yield.The rhizosphere is the'home'for several genes,some of which are responsible for the various trait and features exhibited by plants.Numerous microbes have been identified to inhabit the rhizosphere,some of which are the proteobacteria,nitrogen fixing bacteria,plant growth promoting rhizobacteria,mycorrhizal and myco-parasitic fungi.Likewise,chemical compounds such as amino acids(crystathionine and homoserine),which inhibits nematodes,carbohydrates(fructose),which enhance the growth of microorganisms,and sterols(sitosterol),which regulate plant growth,reside in the plant rhizosphere.Several factors con-tributes to the existence and survival of these microbes,one being good agricultural practices,as some farming techniques negatively affects the soil microbial content.This study therefore focused on the agricultural and health importance of the soil organisms,as well as the various techniques that can be employed to maintain the rhizosphere microbiome,despite the rise and intensification of various harmful land use activities.This will enable a better understanding of the soil rhizosphere conservation,restoration and sustainable use,specifically of degraded land.This will avoid depleting or alternating the soil microbial content while aiming to ensure a healthy ecosystem,biodiversity,maximal crop produc-tivity,food security and all round sustainability,as targeted by the United Nation's Sustainable De-velopments Goals.