Synthesis, Assessment of Biological Activity and Toxicity for N-(<i>β</i>-D-Glycopyranosyl)-Thiosemicarbazides

In the modern science, priority is given for the search of biological active compounds with specific properties. As a result of experimental data, it was found that in the reaction between N-(β-D-glycopyranosyl)-semicarbazide and the Lawesson reagent (2,4-bis(p-methoxyphenyl)-1,3-dithiadiphosphetane 2,4-disulfide) at the ratio 1:1 in pyridine when boiling under reflux in a water bath for 20 - 35 minutes, a new synthetic compound N-(β-D-glycopyranosyl)-thiosemicarbazide is formed. The individuality and structure of the target products were confirmed by 13C NMR spectroscopy, 1H NMR spectroscopy, IR spectroscopy, and elemental analysis. For the synthesized new compounds of N-(β-D-glycopyranosyl)-thiosemicarbazides, the probability of pharmacological and toxic effects were predicted by the computer method in silico. From the synthesized compounds N-(β-D-galactopyranosyl)-thiosemicarbazide, the probability of antibacterial (antibacterial) activity is predicted (Pa/Pi 0.544/0.013). The antibacterial activity of the compound (4) was confirmed in a test for salmonella infection of lambs, salmonellosis of calves, and colipathogenic E. coli serotypes. An experimental study by the in vitro method made it possible to conclude that the new synthetic compound N-(β-D-galactopyranosyl)-thiosemicarbazide in the studied concentrations has a pronounced bactericidal and bacteriostatic effect. The synthetic new compound N-(β-D-glyco- pyranosyl)-thiosemicarbazide is a promising compound for further study.

“Liverscore”is predictive of both liver fi brosis and activity in chronic hepatitis C

AIM:To formulate a noninvasive index predictive of se-verity of liver f ibrosis and activity in chronic hepatitis C.METHODS:This cross sectional study was conducted on polymerase chain reaction positive,treatment nave patients.Fibrosis was staged on a f ive point scale from F0-F4 and activity was graded on a four point scale from A0-A3,according to the METAVIR system.Patients were divided into two overall severity groups,minimal disease(< F2 and < A2)and signif icant disease(≥ F2 or ≥ A2).Eleven markers were measured in blood.Sta-tistically,the primary outcome variable was identif ica-tion of minimal and signif icant overall disease.Indices were formulated using β regression values of different combinations of nine statistically significant factors.Diagnostic performance of these indices was assessed through receiver-operating characteristic curve analysis.RESULTS:A total of 98 patients were included and of these 46 had an overall clinically significant disease.Our f inal six marker index,Liverscore for Hepatitis C,consisted of age,alanine transaminase,gamma-gluta-myl transpeptidase,apolipoprotein A-1,alpha-2 macro-globulin and hyaluronic acid.The area under the curve was found to be 0.813.On a 0-1 scale,negative predic-tive value at a cutoff level of ≤ 0.40 was 83%,while positive predictive value at ≥ 0.80 remained 89%.Al-together,61% of the patients had these discriminative scores.CONCLUSION:This index is discriminative of minimal and significant overall liver disease in a majority of chronic hepatitis C patients and can help in clinical de-cision making.

Stable Silver Nanoparticles Synthesis by Citrus Sinensis(Orange) and Assessing Activity Against Food Poisoning Microbes

Silver nanoparticles are considerecl as good antimicrobial agent. AgNPs were synthesized by mixing silver nitrate solution with citrus sinesb extract for 2 h at 37 and analyzed by UV-visible spectra, SEM, XRD, and FTIR. AgNPs were tested against B. subtilis, Shigello, S. oureus, ond E. coli. Minimum inhibitory concentration of AgNPs was 20 I^g/mL for B. subtilis and Shigello and 30 I^g/mL for S. oureus and E. coll. Antibiofilm activity （80% to 90%） was observed at 25 IJg/mL. AgNPs were stable for five months with sustained an＇timicrobial activity. Biosynthesized AgNPs can bE： used to inhibit food poisoning microbial growth.

A quantification of the treadmill 6-min walk test using the My Wellness Key^(TM) accelerometer

Background： Accelerometers have been suggested to provide additional information during the 6-min walk test which may be useful in evaluating changes in functional exercise capacity. The aim of the study was to identify whether the additional information measured by the My Wellness Key^TM（MWK） accelerometer during a treadmill 6-min walk test（t-6MWT） was related to currently used outcome measures.Methods： Fifteen participants（9 males, 6 females） performed a self-paced t-6MWT. Respiratory gas analysis and walking distance were measured whilst wearing the MWK.Results： A significant correlation was established between activity counts and 6-min walk distance（6MWD）（r=-0.847, p 〈 0.001） yet not 6-min walk work（6MWW）（r=-0.337, p=0.220）. Energy expenditure estimated by the MWK was strongly correlated to 6MWW（r=0.938, p 〈 0.001）but not 6MWD（r=0.477, p=0.072）. The MWK significantly underestimated energy expenditure（36.73, CI=33.9-39.7 kcal） compared to gas analysis（54.35, CI=46.2-61.4 kcal） demonstrating poor agreement between the two analyses（Bias=-17.61 kcal, Limits of agreement=-37.4,t2.2 kcal）. Measurement of time spent undertaking light, moderate, and vigorous physical activity was not significantly different（ p 〉 0.05）between the MWK and gas analysis.Conclusion： Estimated energy expenditure provided by the MWK was strongly correlated to 6MWW; however, MWK underestimated energy expenditure as measured by gas analysis. The MWK may provide outcome data that supplement those currently provided by the 6MWD for functional capacity assessment during the t-6MWT.

The Function and Meaning of Receptor Activator of NF-κB Ligand in Arterial Calcification

Osteoclast-like cells are known to inhibit arterial calcification. Receptor activator of NF-κB ligand（RANKL） is likely to act as an inducer of osteoclast-like cell differentiation. However,several studies have shown that RANKL promotes arterial calcification rather than inhibiting arterial calcification. The present study was conducted in order to investigate and elucidate this paradox. Firstly,RANKL was added into the media,and the monocyte precursor cells were cultured. Morphological observation and Tartrate resistant acid phosphatase（TRAP） staining were used to assess whether RANKL could induce the monocyte precursor cells to differentiate into osteoclast-like cells. During arterial calcification,in vivo and in vitro expression of RANKL and its inhibitor,osteoprotegerin（OPG）,was detected by real-time PCR. The extent of osteoclast-like cell differentiation was also assessed. It was found RANKL could induce osteoclast-like cell differentiation. There was no in vivo or in vitro expression of osteoclast-like cells in the early stage of calcification. At that time,the ratio of RANKL to OPG was very low. In the late stage of calcification,a small amount of osteoclast-like cell expression coincided with a relatively high ratio of RANKL to OPG. According to the results,the ratio of RANKL to OPG was very low during most of the arterial calcification period. This made it possible for OPG to completely inhibit RANKL-induced osteoclast-like cell differentiation. This likely explains why RANKL had the ability to induce osteoclast-like cell differentiation but acted as a promoter of calcification instead.

Progress and Perspectives of Ecosystem Services: A Review

According to the latest research of ecosystem service（ ES）,the background and connotation of ecosystem services were briefly summarized,the relationship among human activity,climate change,and biodiversity and ecosystem service function（ ESF） was synthetically analyzed,the research trends of ecosystem services function assessment（ ESFA） were discussed from multi-scale,and the perspectives was given based on the past studies. The ecological long-term location monitoring method based on the ESFA should be studied,evaluation index of ESFA should be further improved,and the ESF research on the arid region should be reinforced.

Assessment of VIIRS 375 m active fire using tropical peatland combustion algorithm applied to Landsat-8 over Indonesia’s peatlands

VIIRS 375 m active fire data(VNP14IMG),the highest spatial resolution available cost-free fire product,were assessed for representing fire in typical degraded tropical peatlands in Indonesia.The results of applying the Tropical Peatland Combustion Algorithm to Landsat-8(ToPeCAl-L8)daytime imagery were utilised as the fire references.To permit the comparison of non-simultaneous VNP14IMG and ToPeCAl-L8,peatland fire propagation speeds resulting from previous study using TET-1 data in Central Kalimantan’s peatlands were utilised.Most peatland fires were still within 750 m from their source over 15 h under uniform conditions,except for very large fires.The detection rates of nighttime VNP14IMG compared with ToPeCAl-L8 showed about 80%agreement for small fire areas(<14 ha).For fires larger than 14 ha,a dissolved 375 m buffer(cluster buffer)of VNP14IMG active fires with an integration of nighttime and daytime acquisitions,produced a probability of detection up to 90%.These results generated a recommendation for implementing cluster buffer analysis and integration of nighttime and daytime analysis of VNP14IMG data for better accuracy in fire detection for ground fire management.They also demonstrate the utility of the ToPeCAl-L8 algorithm with VIIRS 375 m active fire data.

Evaluation of bacterial pathogen diversity,abundance and health risks in urban recreational water by amplicon next-generation sequencing and quantitative PCR

The microbial quality of urban recreational water is of great concern to public health.The monitoring of indicator organisms and several pathogens alone is not sufficient to accurately and comprehensively identify microbial risks.To assess the levels of bacterial pathogens and health risks in urban recreational water,we analyzed pathogen diversity and quantified four pathogens in 46 water samples collected from waterbodies in Beijing Olympic Forest Park in one year.The pathogen diversity revealed by 16 S r RNA gene targeted next-generation sequencing（NGS） showed that 16 of 40 genera and 13 of 76 reference species were present.The most abundant species were Acinetobacter johnsonii,Mycobacterium avium and Aeromonas spp.Quantitative polymerase chain reaction（q PCR） of Escherichia coli（uid A）,Aeromonas（aer A）,M.avium（16S r RNA）,Pseudomonas aeruginosa（oaa） and Salmonella（inv A） showed that the aer A genes were the most abundant,occurring in all samples with concentrations of 10^（4–6） genome copies/100 m L,followed by oaa,inv A and M.avium.In total,34.8% of the samples harbored all genes,indicating the prevalence of these pathogens in this recreational waterbody.Based on the q PCR results,a quantitative microbial risk assessment（QMRA） showed that the annual infection risks of Salmonella,M.avium and P.aeruginosa in five activities were mostly greater than the U.S.EPA risk limit for recreational contacts,and children playing with water may be exposed to the greatest infection risk.Our findings provide a comprehensive understanding of bacterial pathogen diversity and pathogen abundance in urban recreational water by applying both NGS and q PCR.